Transient transfection of the α2(I) collagen promoter in hepatic stellate cells exposed to 200 μM acetaldehyde, MDA, or both. Two to ten μg of the wild-type (pGL3-1009) promoter was transfected by the calcium phosphate precipitation method  or by Lipofectamine. Exposure time to all culture additives was 48 h. Experiments shown were performed in triplicate on three separate occasions and transfection efficiency was assayed by cotransfection with 0.5 μg β-galactosidase. The data shown represent the average of these studies ± SE. Data analysis was by Student’s t-test. Results shown were standardized to protein assayed (RLU/μg protein). Protein sample analysis by the method of Lowry . No significant difference exists between treatment groups, but a significant difference (p < .05) did exist between each treatment group and untreated, or control lysates.