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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Cell Cycle. Author manuscript; available in PMC 2010 August 24.
Published in final edited form as:
Published online 2007 August 13.
PMCID: PMC2927486
NIHMSID: NIHMS227964

Stimulus-Specific Transcriptional Regulation Within the p53 Network

Abstract

The p53 transcriptional network is composed of hundreds of effector genes involved in varied stress-response pathways, including cell cycle arrest and apoptosis. It is not clear how distinct p53 target genes are differentially activated to trigger stress-specific biological responses. We analyzed the p53 transcriptional program upon activation by two DNA-damaging agents, UVC and doxorubicin, versus the non-genotoxic molecule Nutlin-3. In colorectal cancer cells, UVC triggers apoptosis, doxorubicin induces transient cell cycle arrest followed by apoptosis, and Nutlin-3 leads to cell cycle arrest with no significant apoptosis. Quantitative gene expression analysis allowed us to group p53 target genes into three main classes according to their activation profiles in each scenario. The CDK-inhibitor p21 was classified as a Class I gene, being significantly activated under cell cycle arrest conditions (i.e., doxorubicin and Nutlin-3) but not during UVC-induced apoptosis. Chromatin immunoprecipitation analysis of the p21 locus indicates that the level of p53-dependent transcription is determined by the effects of stimulus-specific transcriptional coregulators acting downstream of p53 binding and histone acetylation. In particular, our analysis indicates that the subunits of the CDK-module of the human Mediator complex function as stimulus-specific positive coregulators of p21 transcription.

Keywords: p53, CDK8, Mediator, general transcription factors, DNA damage, Nutlin, RNA polymerase, transcription, elongation, phosphorylation

INTRODUCTION

The p53 transcription factor functions as a signaling hub of pivotal importance for cellular stability and transformation. Multiple upstream signaling pathways lead to p53 activation, including those induced by potentially oncogenic events, such as DNA damage, oncogene hyperactivation, telomere erosion, nutrient deprivation and hypoxia. Activated p53 participates in the orchestration of varied cellular responses to these stressful stimuli, such as cell cycle arrest, apoptosis or senescence, which in turn generates a strong selective pressure to inactivate this protein during cancerous growth. Accordingly, p53 is mutated in about half of all human cancers and is considered to be the most commonly mutated tumor suppressor gene.13

The p53 protein functions primarily as a transcriptional regulator. Recent analysis of p53 binding to chromatin on a genome-wide scale revealed that the total number of p53 target genes may reside in the several hundreds, including protein-coding genes as well as non-coding RNAs.4,5 Seminal microarray experiments clearly defined the existence of stimulus-specific p53-dependent transcriptional programs. Zhao et al. demonstrated that, depending on the way p53 was activated (e.g., DNA damage caused by ionizing versus non-ionizing radiation), different subsets of p53 target genes were activated.6 In these experiments, the set of p53 target genes activated in all signaling scenarios tested represented a very small fraction of the total p53 transcriptional program. These observations reiterate one of the most challenging questions in the p53 field: What are the mechanisms driving differential regulation of p53 target genes? In the past few years, several models have been put forward to explain this phenomenon. One model proposes that post-translational modifications of p53 play a critical role in this process. For example, phosphorylation and/or acetylation of specific residues in the p53 protein have been shown to enhance its ability to activate pro-apoptotic genes in certain experimental conditions.7,8 However, it is not known how specific p53 post-translational modifications may affect its transactivation potential in a gene-specific manner. Another model proposes that the ability of p53 to recognize response elements on specific genes is modulated by p53 `cofactors' such as the p53-binding protein ASPP1 or the p53-related proteins, p63 and p73.9,10 Other views would suggest that the quality of the p53-dependent transcriptional program is affected by other transcriptional regulators acting in parallel pathways and modulating subsets of p53 target genes. For example, the oncogenic transcription factor c-myc promotes the apoptotic response by specifically repressing transcription of the p21 gene, a key mediator of p53-dependent cell cycle arrest.11 A modest review of the literature suggests that not one single model can reconcile all the observations generated so far (for an excellent review of these issues see ref. 12). It is likely that multiple mechanisms, both known and undiscovered, contribute to define the ultimate p53-dependent transcriptional profile provoked by a given p53-activating stimulus. In this report, we summarize recent findings from our group as well as present new data that generate further insight into the mechanisms of stimulus-specific regulation of p53-dependent transcription.1214 We found that the ultimate activation status of p53 target genes can be defined at steps downstream of p53 binding to chromatin and p53-mediated recruitment of histone-modifiying activities. Our studies reveal the existence of stimulus- and gene-specific transcriptional coregulators acting within the p53 network, including subunits of the Mediator complex, as well as specific general transcription factors (GTFs) and elongation factors.

MATERIALS AND METHODS

Cell culture, FACS Analysis of Cell Cycle Profile, Q-RT-PCR, Western Blots and ChIP assays were performed as described in refs. 13 and 14. Primer sequences for HDM2 Q-RT-PCR are described in ref. 14. Primer sequences for Q-RT-PCR analysis of APAF1 and GADD45 are: APAF1 sense: 5'-CCTAGGCGCAAAGGCTTG-3'; APAF1 antisense: 5'-GATCTTTCTCTCTCTGAGCTGTCAAC-3'; GADD45 sense: 5'- ATTCTCGGCTGGAGAGCAGA-3'; GADD45 Antisense: 5'-GCATCCCCCACCTTATCCAT-3'.

RESULTS

Stimulus-specific regulation of p53 target genes

In order to study the mechanisms driving stimulus-specific regulation of p53 target genes, we set out to investigate the p53 transcriptional response in a single cell type subjected to distinct p53-activating stimuli. We chose to employ the well-characterized human colorectal carcinoma cell line HCT116 and three well-known p53-activating agents: Ultra Violet Light C (UVC), the chemotherapeutic agent doxorubicin, and Nutlin-3, the small molecule inhibitor of the p53 repressor HDM2.15 When treated with 25 J/m2 of UVC, 0.5 μM doxorubicin or 10 μM Nutlin-3, HCT116 cells display identical accumulation of p53 protein levels, both in terms of overall fold induction and kinetics of activation.12,14 However, only UVC and doxorubicin induce DNA-damage-mediated post-translational modifications of p53, such as serine phosphorylation, which are not observed in Nutlin 3-treated cells.12,14,16 Interestingly, these three p53-activating agents induce different cellular responses in this cell line (Fig. 1A). UVC induces apoptosis, as evidenced by a significant increase in the subG1 population at 48 h post-irradiation. UVC-induced apoptosis is partially p53-dependent, as apoptosis is decreased, but not absent, in the HCT116 p53−/− isogenic cell line.14 Doxorubicin induces a well-characterized cell cycle arrest response, with most cells stalling at the G2/M boundary.13,17 At later time points (48 h and beyond), doxorubicin-treated cultures show clear signs of apoptosis (Fig. 1A).17 In this scenario, p53 plays an anti-apoptotic role, as the lack of p53 results in less cell cycle arrest and increased apoptosis.17 Finally, Nutlin-3 induces a cell cycle arrest response with cells accumulating in both G1 and G2/M. This response is fully dependent on p53, as it is not observed in HCT116 p53−/− cells, and is also completely reversible, as cells resume proliferation once Nutlin-3 is removed from the cell culture media.14,16,18 Importantly, there is no significant apoptosis in HCT116 cells upon Nutlin-3 treatment (Fig. 1A). In summary, UVC, doxorubicin and Nutlin-3 induce different cellular responses involving p53 activation.

Figure 1
Stimulus-specific regulation of p53 target genes. (A) Distinct p53-activating stimuli induce different cellular responses. Subconfluent cultures of HCT116 p53+/+ cells were left untreated (None) or treated with 25 J/m2 of UVC, 0.5 mM doxorubicin or 10 ...

Next, we aimed to identify differences in the p53-dependent transcriptional program evoked by UVC, doxorubicin and Nutlin-3. Toward this end, we employed Q-RT-PCR assays to measure induction of known p53 target genes in each scenario. Analysis of more than twenty p53 target genes allowed us to identify three different groups of genes based on their distinct expression profiles (Fig. 1B). One group, represented here by HDM2 and referred hereto as Class I, was maximally activated in response to Nutlin-3, significantly activated by doxorubicin and very modestly activated by UVC. The p21 gene, a key contributor to p53-dependent cell cycle arrest, also belongs to this class and shows an expression profile identical to that of HDM2 (data not shown, see refs. 13 and 14). Class II genes, represented here by APAF1, were maximally induced by doxorubicin, modestly induced by Nutlin-3 and not induced at all by UVC. Finally, Class III genes were activated to a similar extent by all three agents, as illustrated here by GADD45. These results confirmed the flexibility of the p53 transcriptional program and allowed us to begin more mechanistic studies. We decided to focus on the Class I gene p21. Importantly, p21 protein levels correlated tightly with the cell fate choice adopted in our paradigm, displaying significant accumulation in arrested cells (i.e., upon treatment with doxorubicin or Nutlin-3) and no expression in cells undergoing UVC-induced apoptosis (Fig. 1C).

Stimulus-specific transcriptional complexes acting on the p21 locus

We hypothesized that differences in p21 mRNA accumulation were due to stimulus-specific transcriptional events at the p21 locus. To test this, we performed high-resolution, quantitative Chromatin Immunoprecipitation assays (ChIP) using antibodies against p53, histone modifications, RNAP II, GTFs and Mediator. Table 1 shows a summary of our ChIP analysis. Some of the primary data for this Table can be found in refs. 1214.

Table 1
ChIP analysis reveals the assembly of stimulus-specific transcriptional complexes on the p21 locus

ChIP assays with p53 antibodies showed that p53 binding to the response elements found in the p21 locus increases significantly and to similar extent upon all three stimuli, mirroring total p53 accumulation by Western blot. Furthermore, p53-mediated acetylation of histone H3 lysine 9 and histone H4 lysines 5, 8, 12 and 16 are also equivalent in each scenario. In contrast, RNAP II activation is clearly distinct in UVC-versus doxorubicin- and Nutlin-3-treated cells. Significant levels of paused RNAP II are found at the p21 proximal promoter before stimulation, indicating that this gene is regulated at post-recruitment steps.12,13 After UVC irradiation, pre-loaded RNAP II is lost after a transient wave of transcription.12 In contrast, activation of p53 by doxorubicin or Nutlin-3 leads to a sustained increase of RNAP II occupancy in the p21 intragenic region, which is indicative of active elongation and productive transcription. When using antibodies recognizing the Ser5-phosphorylated form of the RNAP II C-terminal domain repeats (S5P-CTD), a significant increase in the low basal signals is observed only after doxorubicin and Nutlin-3. Ser5 phosphorylation peaks at the 5' end of the gene and is thought to mediate promoter escape by RNAP II.19 Differential activation of RNAP II at the p21 locus is also evident in ChIP assays using antibodies against the Ser2-phosphorylated form of the RNAP II CTD (S2P-CTD). S2P-CTD phosphorylation accumulates toward the 3' end of genes and is a marker of active elongation.19 Significant increases in S2P-CTD signals at the p21 locus are observed after treatment with doxorubicin or Nutlin-3, but not upon UVC irradiation. Overall, these results indicate that the ultimate activation status of the p21 locus is not determined by p53 binding to chromatin or recruitment of histone acetyl-transferases, and that it may instead be defined at other regulatory steps. Additional results from our group indicate that this conclusion is not specific to the p21 locus and it applies to many p53 target genes analyzed so far. To generate further insight into this issue, we analyzed the association of transcriptional coregulators acting at the p21 proximal promoter, including several GTFs and Mediator. These studies revealed the assembly of stimulus-specific transcriptional complexes at the p21 locus. The results from this analysis and their interpretation can be itemized as follows:

(1) Overall, GTFs were easily detectable at the p21 proximal promoter before p53 stimulation, consistent with the observed pre-loading of RNAP II discussed above.1214 In contrast, binding of Mediator subunits was less evident before p53 activation. The fact that significant amounts of RNAP II were present at the p21 promoter before association of the Mediator complex suggests that the latter may regulate post-initiation steps at this locus.

(2) TFIIA seems to sense activation signals arising from p53 activation in every scenario, as it displays enhanced recruitment to the proximal promoter of p21 after all three stimuli. The lack of correlation between recruitment of TFIIA and transcriptional activity suggests it is not a key determinant of the ultimate activation status of p21.

(3) The TATA-binding protein (TBP), a subunit of the TFIID complex, is present at the p21 proximal promoter before p53 activation, maintains its association following UVC and doxorubicin treatment and increases only upon Nutlin-3 treatment. Increased TBP binding may allow for the maximum activation observed during Nutlin-3 treatment.

(4) Enhanced recruitment of the TBP-associated factor 1 (TAF1) is observed only after UVC irradiation. The distinct behaviors of TBP and TAF1 reveal a degree of plasticity in the assembly of the TFIID complex. The fact that TAF1 is the only protein identified so far that is recruited to the p21 promoter preferentially upon UVC irradiation leads us to hypothesize that it may function as a transcriptional repressor in this scenario. Current efforts in our lab aim to test this notion.

(5) TFIIB binding increases significantly with doxorubicin, more modestly with Nutlin-3 and actually decreases with UVC. TFIIB behavior suggests that it is a key positive regulator of p21 transcription. Its decreased binding following UVC may explain the lack of sustained transcription in this scenario. However, the fact that TFIIB binding is maximal upon treatment with doxorubicin rather than Nutlin-3 indicates that it is not the ultimate indicator of p21 transcriptional activity.

(6) TFIIF recruitment increases with doxorubicin and Nutlin-3, but not with UVC. This behavior makes TFIIF a better predictor of the transcriptional status of the p21 locus than TFIIA or TFIIB. Of note is the observation that while the association of other GTFs is restricted to the proximal promoter, TFIIF is also present several kilobases into the 5' end of the intragenic region, perhaps indicative of its role in elongation control.13,20

(7) MED1, a `core' Mediator subunit known to interact directly with p53, is recruited upon treatment with all three stimuli.21 The recruitment profile of MED1 at the p21 locus is interesting in that the increased association of MED1 occurs at both the p53 enhancer elements and at the proximal promoter, and to a lesser extent the 5' end of the intragenic region.14 This occupancy profile supports the idea that Mediator acts as a molecular bridge to connect sequence-specific DNA binding proteins, often acting from distal elements, with the basal transcription machinery acting at core promoters. However, it is important to take note that the recruitment of the core Mediator complex does not determine the ultimate activation status of the p21 locus.

(8) Unlike MED1, subunits of the CDK-Module of Mediator (CDK8, Cyclin C and MED12) are recruited to the p21 locus only during conditions of activation.14 The CDK-module is preferentially recruited to the proximal promoter of p21. In fact, of all of the transcriptional coregulators analyzed, the level of recruitment of CDK8 most closely correlates with the level of p21 transcriptional activation. This suggested that CDK8, a protein often associated with transcriptional repression at other human loci, functions instead as a positive coregulator in this scenario.22,23 We tested this hypothesis using anti-CDK8 siRNAs and reporter assays and confirmed that CDK8 acts as a positive coregulator of p21 and HDM2 expression.14

DISCUSSION

Our systematic analysis of transcriptional complexes acting on the p21 locus under different signaling scenarios has revealed an unexpected role for transcriptional coregulators previously considered to act in a `generic' fashion. Instead, many of them clearly act in a stimulus-specific manner. Our current efforts are aimed to: (1) identify other stimulus-specific coregulators acting on the p21 locus, (2) elucidate which stimulus-specific factors are determinants of RNAP II activity at the p21 locus versus those whose behavior is epiphenomenal to RNAP II activity and (3) analyze other Class I genes, as well as Class II and Class III genes, and identify common and/or differential regulatory patterns. Our analysis of p53 target genes beyond p21 has begun to generate some interesting observations. For example, the pattern of recruitment of transcriptional coregulators to the promoter of HDM2, another Class I gene, is very similar to that observed for p21. Specifically, stimulus-specific recruitment of TFIIB, TAF1 and CDK8 is identical at both loci. Particularly interesting are Class II genes (e.g., APAF1), which are not maximally induced by Nutlin-3 treatment, suggesting that their full activation may require additional events beyond p53 stabilization, such as p53 phosphorylation or the action of other stress-induced transactivators. On the other hand, only Class III genes are effectively induced upon UVC irradiation as compared to Nutlin-3 and doxorubicin treatment. We hypothesize that UVC-mediated DNA damage generates repressive signals that block transactivation of Class I and Class II genes, but not of Class III genes. Of note, doxorubicin and UVC produce different types of DNA damage thus leading to activation of distinct downstream signaling pathways. Doxorubicin is a drug thought to act mostly trough inhibition of topoisomerase II activity, thus producing accumulation of double-stranded breaks and activation of the ATM signaling pathway.2426 In contrast, UVC irradiation produces pyrimidine dimers, which activate the ATR signaling pathway.26 It is unclear how ATM- and/or ATR-dependent signaling pathways may affect the activity of specific transcriptional coregulators acting within the p53 network. It would be of great interest to determine if DNA damage-induced signaling modulates the activity of Mediator or GTF subunits.

The fact that p53 target genes show distinct stimulus-specific patterns of activation suggests the existence of gene-specific regulatory events within the network. Comparative analysis of the transcriptional complexes acting on different p53 target genes may lead to the identification of gene-specific coregulators. In this regard, we recently discovered that CDK9, a positive elongation factor once considered to be essential for all transcription, is required for transcriptional activation of some, but not all, p53 target genes. Current efforts in our lab aim to elucidate how the combinatorial use of the so-called `transcriptional CDKs' (i.e., CDK7, CDK8, CDK9, CDC2L6 and CDK11) may provide flexibility and specificity to the p53 transcriptional program. In summary, we propose that combinatorial use of both stimulus-specific and gene-specific coregulators within the p53 network generates the diversity required to fine tune the p53 transcriptional response to the changing needs of the cell. Deciphering this complex regulatory web will allow the scientific community to envision novel therapeutic strategies aimed to evoke a desired p53-dependent biological response.

Acknowledgements

We thank all members of the Espinosa lab for support and advice, Glen Bjerke for technical assistance, Dr. Lubo Vassilev for Nutlin-3, Dr. Vogelstein for cells and Dr. Layton Kor for guidance. This work was supported by grants from NIH (CA117907), March of Dimes (5-FY05-1217), DOD-CDMRP (CM05054) and SPORE in Lung Cancer. Jennifer Michelle Hoover was supported by the Undergraduate Research Opportunity Program (UROP, CU-Boulder).

ABBREVIATIONS

APAF1
apoptotic peptidase activating factor 1
CDK8
cyclin dependent kinase 8
GADD45
growth arrest and DNA-damage-inducible
GTF
general transcription factor
HDM2
human homolog of mouse double minute 2
Q-RT-PCR
quantitative-reverse transcription-polymerase chain reaction
TBP
TATA-binding protein
TAF1
TBP associated factor 1
TFIIA
RNA polymerase II transcription factor II A
TFIIB
RNA polymerase II transcription factor II B
UVC
ultraviolet light C

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