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Nucleic Acids Res. 2010 August; 38(15): iii.
Published online 2010 August 19. doi:  10.1093/nar/gkq772
PMCID: PMC2926630

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Cover: Quantitative analysis of processive RNA degradation by the archaeal RNA exosome: RNA exosomes are large self-compartmentalized nucleases implicated in processive, controlled degradation of a large variety of RNAs. A combination of structural biology, biochemical assays and Markov chain Monte Carlo based parameter estimation allows robust quantitative analysis of RNA degradation kinetics of exoxome variants and mutants. The illustration shows the crystal structure of the Archaeoglobus fulgidus exosome (RNase PH like core: green; Csl4-cap: orange) with electron density for the RNA bound to the active site (blue). Protein crystals, a close-up of the RNA bound to the active site of the exosome, a sequencing gel of RNA degradation products and the quantitative analysis of degradation are depicted in the background.
For further information, please see paper by S.Hartung et al. Nucleic Acids Res., 2010, 38, 5166–5176.

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press