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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Structure. Author manuscript; available in PMC 2011 August 11.
Published in final edited form as:
PMCID: PMC2925294
NIHMSID: NIHMS224795

Zernike Phase Contrast Cryo-Electron Microscopy and Tomography for Structure Determination at Nanometer and Sub-Nanometer Resolutions

SUMMARY

Zernike phase contrast cryo-electron microscopy (ZPC-cryoEM) is an emerging technique which is capable of producing higher image contrast than conventional cryoEM. By combining this technique with advanced image processing methods, we achieved subnanometer resolution for two biological specimens: 2-D bacteriorhodopsin crystal and epsilon15 bacteriophage. For an asymmetric reconstruction of epsilon15 bacteriophage, ZPC-cryoEM can reduce the required amount of data by a factor of ~3 compared to conventional cryoEM. The reconstruction was carried out to 13 Å resolution without the need to correct the contrast transfer function. New structural features at the portal vertex of the epsilon15 bacteriophage are revealed in this reconstruction. Using ZPC cryo-electron tomography (ZPC-cryoET), a similar level of data reduction and higher resolution structures of epsilon15 bacteriophage can be obtained relative to conventional cryoET. These results show quantitatively the benefits of ZPC-cryoEM and -cryoET for structural determinations of macromolecular machines at nanometer and subnanometer resolutions.

INTRODUCTION

In cryo-electron microscopy (cryoEM), biological specimens, such as purified proteins, virus particles, organelles and cells, are embedded in amorphous ice by rapid freezing (Dubochet et al., 1988; Baker et al., 2009), and directly observed in the microscope, maintaining them at low temperature. The frozen, hydrated specimens can be considered as phase objects in transmission electron microscopy, and the 3-D density function of their contents can be retrieved from the images (Toyoshima and Unwin, 1988).

In a conventional cryoEM, the image contrast of such phase objects is mainly generated by a combination of lens aberration and defocus (Scherzer, 1949). In frequency space, the data are modulated by the sinusoidal contrast transfer function (CTF) and damped by exponential envelope functions, causing weak contrast at low frequency, loss of information at the nodes of the CTF, and a fall-off in signal at high frequency (Hanszen, 1971; Thon, 1971). Because of the electron optics and the low dosage used to record images of frozen, hydrated biological specimens, the image contrast is generally low. The enhancement of signal to noise ratio (SNR) is achieved through extensive data processing by aligning and averaging multiple copies of identical objects. The lower the image contrast, the more difficult it is to align the images accurately. Therefore, low image contrast, especially at low resolution, is a major hurdle in structural determination of small biological objects.

Using the original idea proposed by Zernike to enhance the image contrast in a light microscope (Zernike, 1942), two different approaches have been followed for transmission electron microscopy (Boersch, 1947). One method is to use an electrostatic field generated by an electrode inserted into the back-focal plane of the objective lens, which alters the phase of the unscattered beam that then interferes with the scattered beam to form the image. Recent micro-fabrication technology allows manufacturing of a micron-size device (Cambie et al., 2007; Majorovits et al., 2007; Schultheiss et al., 2006). However, the presence of the electrode produces a distorted image. The other method is to use a thin film made of carbon, with a central hole of sub-micron diameter as a phase plate. This thin film phase plate is also placed in the back-focal plane, with the unscattered beam going through the hole unchanged, and the phase of the diffracted beam shifted by π/2. The π/2 phase shift in the scattered beam changes the CTF from a sine to a cosine function (Nagayama, 2005) and thus substantially enhances the low frequency contrast. Furthermore, the image can be taken at close to zero defocus so that there is no data loss due to the CTF nodes in the data. This approach has been attempted numerous times in the past without success (Badde and Reimer, 1970; Balossier and Bonnet, 1981; Faget et al., 1962; Johnson and Parsons, 1973; Kanaya et al., 1958; Krakow and Siegel, 1975; Parsons and Johnson, 1972; Unwin, 1970; Willasch, 1975). Only recently has the phase plate contamination inside the microscope been partially overcome to make this approach practical (Danev et al., 2009).

Zernike phase contrast cryoEM (ZPC-cryoEM) has been used to image ice-embedded biological specimens including proteins, viruses, and cells, at low and moderate resolutions (Yamaguchi et al., 2008). A single particle reconstruction of a 14-fold symmetric GroEL has been obtained at 12 Å resolution (Danev and Nagayama, 2008). This study showed that the number of images was reduced by ~30% to get the same resolution, compared to a reconstruction from conventional images. Such data reduction is smaller than the theoretical expectation (Chang et al., 2010). In our current study, we combined a state-of-the-art electron cryomicroscope equipped with a C-film phase plate at the focal plane of the objective lens for data collection and data processing method for image reconstruction. We applied it to two well-known structures (2-D bacteriorhodopsin crystals and epsilon15 bacteriophages) and were able to reconstruct the epsilon15 bacteriophage at better resolution with much less data than by using conventional images. New structural features were revealed from the asymmetric reconstruction of epsilon15 by this approach.

RESULTS

2-D crystal analysis of bacteriorhodopsin

Three ZPC-cryoEM images of trehalose-embedded bacteriorodopsin 2-D crystals were collected at close-to-focus conditions on a 4k × 4k CCD camera using a low dose system. Each image contains ~19,000 unit cells. The image shows a characteristic crystal edge but not an obvious crystalline lattice (Figure S1A). However, the computed Fourier transform of the image showed diffraction spots corresponding to the crystal lattice (Figure S1B). The CTF rings are difficult to discern until one computes a 1-D power spectrum using the azimthual average (Figure S1C), wherein we can only detect one CTF minimum in this example. We used the IQ plot (Henderson et al., 1986) to evaluate SNR of the computed intensities of the diffraction spots from the crystal image. The SNR of a diffraction spot is graded by an IQ value ranging from 1 to 8 (1 for the best). Figure 1A shows an example of the IQ plot from a single image, in which the crystal lattice has been unbent to correct for the crystal distortion (Henderson et al., 1986). About 90 % of the reflections out to 8 Å resolution have a ranking better than grade 4.

Figure 1
ZPC 2-D crystal analysis of bacteriorhodopsin

Ideally, the defocus value of an optimal ZPC image should be set close to zero defocus without CTF oscillation till reaching the Scherzer limit. However, our ZPC image still has a CTF oscillation, because it is hard to manually set the specimen focus precisely in the presence of the phase plate. The first CTF zeros (Figure S1C) of the 3 images were found to be between 11 and 9 Å by the ctffind3 program (Mindell and Grigorieff, 2003). The projection map (Figure 1B) was reconstructed from the 3 merged images with P3 symmetry imposed after correcting crystal distortion and CTF in each image. The projection densities of the 7 trans-membrane α-helices can be clearly seen in the resulting map.

After flipping the phases to correct for the CTFs of the 3 images, we evaluated the Fourier amplitudes and phases of the reconstructed projection map. The ratio of the image-derived amplitudes to the electron diffraction amplitudes showed a linear decay to 8 Å (Figure 1C). The slope of the fitting line corresponds to a crystallographic B factor of about 860 Å2. Furthermore, we compared the averaged phases of the diffraction spots from the 3 merged images with the previous study (Henderson et al., 1986). The average phase difference for all diffraction spots out to 8 Å was about 9.75 ± 8.11° and the average phase difference in the range 8 Å to 10 Å was less than 11.26° (Table S1). This result showed that structural information of bacteriorhodopsin was retrievable to 8 Å, which is sufficient to resolve secondary structure elements like transmembrane α helices.

Reconstruction of epsilon15 bacteriophage with icosahedral symmetry imposed

ZPC-cryoEM images of ice-embedded epsilon15 bacteriophages shows high contrast (Figure 2A), and the tail hub and surrounding tail spikes can be clearly seen. The halo surrounding a particle is due in part to the effect of the cut-on frequency of the phase plate hole (Danev et al., 2009). Using the Multi-Path Simulated Annealing (MPSA) refinement procedure (Liu et al., 2007), we reconstructed a 10 Å resolution icosahedral map without a CTF correction from 4,500 good particle images, which were selected from 6,188 raw particle images based on the consistency criterion of MPSA. In contrast to 7–8 iterations generally required by MPSA to refine a structure from scratch with conventional cryoEM images, the entire refinement process for ZPC-cryoEM images from a random model took only four iterations. The central slices of the maps in each of the four iterations (Figures S2A-S2E) show progressively improved structural features.

Figure 2
ZPC single particle reconstruction of epsilon15 bacteriophage with icosahedral symmetry imposed

To extend the resolution beyond 10 Å, we expected that we would have to correct the CTF or eliminate some high-defocus particle images from the data set. However, the CTF rings were not apparent, compared to the case of the 2D crystal images (Figure S1B). We implemented a new method, cross-common-line correlation analysis (see Experimental Procedures), to determine the first CTF zero of each individual particle image. The correlation curve (Figure S2F) was calculated from the common lines between a raw particle image and the 2D references projected from the 10 Å density map, which was reconstructed from the 4,500 particle images without making any CTF correction. When the correlation curve becomes negative, it presumably suggests that the CTF has passed the first CTF zero cross-over (Figure S2F) and thus no longer agrees with the 10 Å model map. About 1,600 particle images whose first CTF zeros fall below 1/10 Å−1 were removed from the reconstruction because of the uncertainty in determining the CTF zeros beyond the first one with confidence. Including only the remaining 2,900 particle images without any CTF flip, we improved the map’s resolution to 9 Å (Figures 2B and 2C). In this subnanometer resolution map, long alpha helices and large beta sheets of the capsid subunits (gp7) can be visualized and match well with the established backbone model (PDB: 3C5B) (Jiang et al., 2008) (Figure 2D).

Reconstruction of epsilon15 bacteriophage with no symmetry imposed

We boxed out the same particles with a larger image size than that used for the icosahedral reconstruction in order to include the phage tail. We applied the orientations of the 5,600 good particle images (from the third icosahedral iteration described above) to the corresponding large-box particle images. Using a newly developed asymmetric reconstruction procedure in MPSA (Liu et al., 2010b), we were able to reconstruct a 13 Å density map without imposing symmetry and without any CTF correction (Figure 3 and Figure S3). This asymmetric reconstruction was done without using a known epsilon15 tail model, to eliminate model bias (Liu et al., 2010b). The capsomere features of the major protein (gp7) and the dimeric feature of the capsid stapling protein (gp10) were clearly visible in this 13 Å asymmetric density map as expected (Jiang et al., 2008).

Figure 3
ZPC single particle reconstruction of epsilon15 bacteriophage with no symmetry imposed

The portal vertex complex, located at one of the 12 five-fold vertices, contains several proteins: tail hub, tail spikes, portal and core proteins (Figures 3E-3H). This map reveals several new structural features, which were not resolved in the previous 20 Å asymmetric reconstruction (Jiang et al., 2006). Figure 3A clearly shows that the tail hub has 6 subunits surrounded by the 6 tail spikes. The tail spike consists of two domains shaped like a bent arm (Figure 3E); the proximal stem domain connected to the tail hub, and the distal flower-like domain, connected by an elbow. Figure 3F shows that the low-pass filtered tail spike has trimeric features viewed from the top. In fact, the entire flower-like domain is clearly split into 3 subunits, a feature which was not observed previously (Jiang et al., 2006).

As shown previously that the portal protein has a 12 fold symmetry (Jiang et al., 2006), we applied a 12-fold symmetry to the portal density (Figure 3G). Its subunit structure is topologically similar to the 9 Å map of the cyanophage P-SSP7 (Liu et al., 2010a). The long “leg” density seen in the portal subunit (arrow in Figure 3H) appears to be analogous to the Q-rich motif helix near the C-terminal end of the P-SSP7 portal protein, which may play a role in the DNA release during phage infection.

Estimation of the “crystallographic” B factor from sub-datasets in the asymmetric reconstruction

Based on the theoretical formula (Rosenthal and Henderson, 2003), Nasym unit = [left angle bracketSright angle bracket2 /left angle bracketNright angle bracket 2•30π / Neσ ed] •eB /2d2 , which shows the relationship between resolution (d), number of asymmetric units (Nasym unit), criterion for the signal-to-noise ratio on amplitudes (<S>2/<N>2), electron dose (Ne), electron scattering cross section (σe) and crystallographic B factor of the data set, the B factor can be estimated from the slope of the curve of the number of asymmetric units versus resolution in different independent reconstructions (i.e. B= 2•Δ(ln(Nd))/Δ(1/d2)), here Δ stands for the difference of the variable.

We generated 7 subsets of data containing 3,000, 2,000, 1,000, 500, 300, 200 and 100 particles, randomly selected from the entire raw single particle image dataset. Following the same asymmetric reconstruction procedures as above, we reconstructed asymmetric maps for each sub-dataset. Surprisingly, after 4 iterations of refinement, all 7 sub-datasets had converged to their final icosahedral reconstructions from a random initial model. For conventional image data, we generally required over 7 iterations before a converged map can be reached. For the asymmetric reconstruction, except for the 100-particle dataset, each of the datasets took only 4 more iterations to converge from an initial model generated from the icosahedral reconstruction (Liu et al., 2010a). Very unexpectedly, we were able to reconstruct a 26 Å map using only 100 particle images, without imposing any symmetry. Since a random model was used as the initial model for each reconstruction, no model bias is introduced (Liu et al., 2007). Typical reconstructions are shown in Figures 3B–3D. We plotted the relationship (Figure 3I) between the number of asymmetric units and the resolution of the map from all 8 independent asymmetric reconstructions. From the slope of the curve in Figure 3I, we estimated the “crystallographic” B factor (Rosenthal and Henderson, 2003) of the particle images to be 1200 Å2. In such an estimate, it is important to use an unbiased initial template for each refinement, as was done here.

Cryo-electron tomography of epsilon15 bacteriophages

An alternative method to obtain an asymmetric structure is to use cryo-electron tomography. The tilt series of the ice-embedded epsilon15 bacteriophage recorded in the ZPC electron microscope showed high contrast images with a varying ripple around the particles (and all the other features of the images) derived from the cut-on frequency of the phase plate hole. The tomographic reconstruction directly delineated structural details of the individual bacteriophage (Figure 4A; Movie S1). We extracted 132 epsilon15 subtomograms from a single tomogram. We made sub-datasets with various numbers of subtomograms randomly selected from the total 132 subtomograms. For each sub-dataset, we used the tomohunter program (Schmid and Booth, 2008) to align the subtomograms in 3D space, and then averaged them with and without icosahedral symmetry. To avoid model bias while aligning the subtomograms, we did not use any known model as a reference. The resolutions of all the sub-datasets using the 0.5 FSC criterion are illustrated in Figure 4B and 4C. It is noted that resolution of the averaged maps in either case (asymmetric and icosahedral) using the ZPC-cryoET data is not much improved when more than 50 subtomograms were used. The averaged map without any enforced symmetry clearly exhibited the features of the tail and the capsid shell (Figure 4D). The dimeric nature of the minor capsid protein, gp10, became visible in the icosahedral symmetry imposed map after the subtomogram averaging (arrow in Figure 4E).

Figure 4
ZPC cryo-ET and subtomogram averaging of epsilon15 bacteriophages

Reconstruction resolution vs. particle image numbers in conventional cryoEM

In order to assess the effects of ZPC-cryoEM on the numbers of particles needed to reconstruct the structure at a given resolution, we used the same instrument to collect conventional images of ice embedded epsilon15 bacteriophage particles and used the same software and procedures to perform both single particle and tomographic reconstructions. In either case, it is consistently found that the numbers of particles required for the same resolution reconstructions are reduced by a factor of ~3 for ZPC relative to the conventional images (Figures 3I, 4B and 4C).

DISCUSSION

The theoretical advantages of ZPC-cryoEM are manifold, as previously enumerated (Nagayama, 2005). The study using GroEL as a test specimen showed the fidelity of the reconstruction to 12 Å from ZPC electron images (Danev and Nagayama, 2008). Our studies demonstrate practically attainable structures with images collected from the state-of-the-art Zernike phase contrast electron cryomicroscope, and also reveal previously unseen structural features in a large virus, using fewer particle images than those from the conventional method.

CTF/Defocus issues in subnanometer reconstructions using ZPC-cryoEM

For ZPC imaging, the optimal defocus is always intended to be close to zero. However, it is hard to achieve this in practice. In our experiment, the defocus was manually adjusted using the live FFT function of the CCD camera control software. The CTF rings are barely visible at low defocus. As an alternative method, we tried to estimate the in-focus from a high defocus setting, where the CTF rings are more readily visible. However, this did not work because the hysteresis of the objective lens makes it difficult to return to the intended defocus. Therefore, it is very difficult to routinely and reliably obtain an image of a specimen in-focus with the current ZPC-cryoEM setting. Presently, to reconstruct a high-resolution map using ZPC, it is necessary to either correct the CTF for each image as done with bacteriorhodopsin, or discard the “bad” defocus images computationally as done with epsilon15 bacteriophage.

The bacteriorhodopsin 2-D crystal offers an ideal specimen to assess the reliability of the reconstruction obtained from the ZPC images because its amplitudes and phases are known (Henderson et al., 1986). Among the three images of bacteriorhodopsin analyzed, there is no need to flip the CTF phases up to 1/11 Å−1. Beyond that, we had to determine the CTF parameters of the image, and the first zero of the CTF ring is barely visible (Figure S1C). However, the accuracy of the phases determined in our study gives a direct verification of its accuracy for 2-D reconstruction up to 8 Å resolution (Table S1).

In the case of single particle ZPC-cryoEM images, the CTF rings of the power spectrum of the summed particle images are not obvious. There exists no algorithm that can determine the CTF parameters of each individual particle in the absence of visible CTF rings when the particle structure is not known. Our method of using a cross-common-line correlation curve (Figure S2F; Experimental Procedures) is used to detect the position of the first CTF zero. Instead of correcting the CTF of the images, we used this information to remove those particle images whose first CTF zero falls below that spatial frequency. To find the correlation curve of a raw image, we used the 10 Å map as a reference, which is the best map that can be reconstructed without considering the CTF; thus we were able to eliminate images whose first CTF zero is at 1/10 Å−1 or lower frequency. The resolution of the map generated from the selected particle images was then improved to ~9 Å. The fact that a subnanometer resolution single particle structure can be obtained from the ZPC-cryoEM is fairly encouraging because the structure at this resolution can reveal long alpha helices and large beta sheet (Figures 1C and and2D).2D). Such structural features assure the reliability of the reconstruction and allow the fitting of the density to the known structures of the molecular components with a higher level of confidence. The ability to achieve subnanometer resolution, combined with the enhancement of image contrast of ZPC-cryoEM, leads to the hope that subnanometer resolution is also possible for smaller proteins (<100 kDa) or small protein components in a large macromolecular assembly whose contrast is too low to detect in conventional cryoEM (Danev et al., 2009; Henderson, 1995).

Limiting factors in subnanometer resolution reconstructions for ZPC-cryoEM

In order to address the question of why the reconstructions of bacteriorhodopsin and epsilon15 were limited to ~8–9 Å, we evaluated the crystallographic B factor in both cases. For bacteriorhodopsin, we could determine this factor by comparing the known electron diffraction amplitude to our computed diffraction amplitude from the images (Henderson et al., 1986). In the case of epsilon15 bacteriophage, we did multiple reconstructions with different numbers of particles and determined their resolutions. The slope of the curve between number of particle images and resolution produced the crystallographic B factor using a theoretical formula (Rosenthal and Henderson, 2003). Using these methods, the crystallographic B factors were estimated to be ~860 and ~1200 Å2 for bacteriorhodopsin 2-D crystal and the single particle epsilon15 bacteriophage images respectively. Such B-factor values would limit the structural determination beyond the current resolution in the reconstruction (Henderson et al., 1986; Liu et al., 2007).

The determined crystallographic B factors in both cases are larger than that one would normally observe in conventional cryoEM reconstructions. In this study, we used a Tietz 4k × 4k CCD camera as a recording medium because emulsion film was thought to generate contaminants in the microscope that would shorten the lifetime of a phase plate. The ZPC images of 2D crystals and single particles were recorded at 75,000× detector magnification (1.95 Å per pixel). The retrievable resolutions for typical reconstructions are expected to be between 2/5 and 2/3 Nyquist resolution depending on the type of CCD camera (Booth et al., 2004; Chen et al., 2008). Therefore, the modulation transfer function of the CCD camera could have been a source of resolution limitation in this case. However, the following independent experimental results indicate that the CCD camera is not a limiting factor in the current 8–9 Å resolution limit. The first experiment was the failure to improve the resolution of the 2-D reconstruction of bacteriorhodopsin even with images taken at a higher magnification. The second experiment was based on visibility of the CTF ripples beyond 8 Å resolution from the conventional CCD images of epsilon15 bacteriophages taken at the same magnification (Figure S4).

Furthermore, from two independent sets of experiments, we found that the crystallographic B factors of ZPC-cryoEM images are larger than those of conventional images recorded by the same CCD camera in the same microscope. We first estimated the B-factors from the fall-off of the CTF rings, calculated from images of an amorphous carbon film (Saad et al., 2001). The B-factor of the phase plate data taken near zero defocus is ~30% higher than that of the conventional images taken at 1–2 μm defocus (data not shown). We alternatively obtained the B-factors from multiple independent reconstructions of epsilon15 bacteriophage with different number of particles (Henderson et al., 1986; Liu et al., 2007). As shown in Figure 3I, the crystallographic B factor of images using the phase plate is ~20% higher than those without a phase plate. This shows the current limitation of phase plate technology and highlights the need for improvement. Possible causes for the relatively high B-factor in ZPC images include charging (Danev et al., 2009) and variability in film thickness of the phase plate.

ZPC-cryoEM for reconstruction without imposed symmetry

There are two factors that affect the resolution of an asymmetric reconstruction, the accuracy of the determined asymmetric orientations and the number of raw particle images. ZPC-cryoEM could help with both these factors. Because there is no loss of structural information due to the CTF oscillation in the ZPC-cryoEM images, it is anticipated that ZPC-cryoEM will require fewer images compared to conventional images to assure full data coverage in Fourier space for image reconstruction. In addition, the high image contrast allows easier and more accurate particle orientation determination. In order to evaluate fairly the reduction in the amount of data required for single particle 3D reconstruction, we collected epsilon15 data using the same electron microscope and CCD camera with and without a phase plate, and processed the images with the same refinement programs. However, in contrast to the in-focus imaging of ZPC-cryoEM, we had to collect the conventional (without phase plate) images at a defocus ranging from 1–3 μm, which is the normal defocus range for conventional cryoEM. Figure 3I clearly shows that ZPC-cryoEM requires ~3-fold less data for the same resolution of the single particle reconstruction over a broad range of resolutions, compared with the conventional cryoEM. In addition to requiring less data, Figures 3E–3H demonstrate convincingly that several of the structural features of the portal vertex complex of epsilon15 are better resolved than the ones in previous cryoEM asymmetric reconstruction with conventional images (Jiang et al., 2006).

Even though it is still technically tedious to obtain images with the current phase plate technology, a moderate resolution (9–30Å) can provide very valuable structural information for understanding a complex macromolecular machine, which can be achieved without correcting CTF. Eliminating the need for correcting CTF will remove a huge technical barrier in using cryoEM as a routine structural tool.

Benefits of ZPC-cryoET

CryoET is a method that generates a low resolution map from a single specimen. Therefore, the highly visible contrast of ZPC-cryo-ET would help to retrieve features from such data. In this study, we used epsilon15 bacteriophage as a test specimen because of its known structure. To find out by how much the SNR of ZPC-cryoET reconstructions can be enhanced, we averaged 132 subtomograms extracted from a single tomogram reconstruction. We also processed different sub-datasets with different numbers of subtomograms randomly chosen from the 132 subtomograms. Figures 4D and 4E show that using only 50 subtomograms can yield an average density map with features including major capsid shell, capsid stapling proteins and all the molecular components of the portal vertex complex. The high structural similarity to that from the single particle reconstruction validates the power of this approach. When we compared the resolution vs. number of subtomograms averaged (Figures 4B and 4C), the resolution improvement slows down with 50 or more subtomograms. This phenomenon may be explained as the CTF effects starting to become significant in this set of data. However, the CTF starts to take effect at lower resolution in the conventional cryoET because of the large defocusing required for increasing the contrast. Therefore, the ZPC-cryoET offers obvious advantages for obtaining low resolution structures without requiring CTF corrections. Nevertheless, it is likely that we will need to make such corrections for reconstruction beyond 20 Å because of the defocus gradient in the tilted specimens. Without correcting the CTF, 10 subtomograms of ZPC-cryoET generated an averaged map at 38 Å resolution, which can only be obtained by using more than 50 subtomograms from conventional cryoET. Therefore, a potential application of ZPC-cryoET is for cells and organelles where there may be no possibility of averaging identical objects to boost the SNR as shown here with the bacteriophage. The enhanced contrast in ZPC-cryoET may reveal some subcellular components without post-tomographic averaging which cannot be seen using conventional cryoET (Danev et al., 2010). Our study has demonstrated that ZPC-cryoEM and cryoET have the potential to carry this imaging modality into the next era of research, by bridging cellular and molecular structure at an unprecedented level of resolution.

EXPERIMENTAL PROCEDURES

Sample preparation

2-D bacteriorhodopsin crystals were prepared from Halobacterium salinarum S9, and fused with octylglucoside and dodecyl trimethylammonium to make larger crystals (Baldwin and Henderson, 1984). The 2-D crystals were embedded in 3% trehalose on a thin carbon film supported by a 300-mesh copper grid (Glaeser and Downing, 1990; Hirai et al., 1999). The sample grid was quickly frozen in liquid nitrogen before it completely dried, and stored in a cryo-storage tank.

Epsilon15 bacteriophage particles were purified from infected Salmonella anatum bacterial culture using sucrose gradient centrifugation as described previously (Jiang et al., 2006). R 1.2/1.3 Quantifoil grids (Quantifoil Micro Tools GmbH, Germany) chemically cleaned with acetone beforehand, were glow discharged for 10 s, and covered with a thin layer of carbon support film. 3 μl of epsilon15 bacteriophage suspension was applied onto the Quantifoil grid, blotted with Whatman 1 filter paper at 95% humidity and room temperature, and quickly frozen in liquid ethane cooled by liquid nitrogen using a Vitrobot (FEI Company, USA). For cryo-ET, 150 Å gold fiducial markers (Electron Microscopy Sciences, USA) were added in the sample aliquot at 10% of the total volume before freezing. The frozen grids were stored in a liquid nitrogen cryo-storage tank.

Image acquisition of bacteriorhodopsin crystals and epsilon15 bacteriophages

The frozen, hydrated grids were loaded onto a Gatan cryoholder (model 914, Gatan Inc., USA), and imaged with a JEM2200FS electron microscope (JEOL Inc., Tokyo) at liquid nitrogen specimen temperature. The instrument used in the present studies was a 200 kV electron microscope with a field emission gun, an in-column energy filter, and a turbo molecular pump. A Zernike phase plate which consists of ~240 Å thick amorphous carbon film with a central 0.5 μm pin-hole, was mounted on each hole of the 5 × 5 array aperture disc (Danev et al., 2009). Four aperture discs are simultaneously mounted on the special aperture holder implemented with a ceramic heater, and sustained in the back-focal plane of the objective lens. The discs were kept at 200 ºC at all times to avoid contamination. The ZPC-cryoEM images were recorded on a Tietz 4k × 4× SlowScan CCD camera (15 μm pixel size) cooled to −25 °C (TVIPS, Germany).

The ZPC images of the 2-D bacteriorhodopsin crystal were collected with a dose rate of 20 electrons per Å2 with a one second exposure time at nominal detector magnifications of 75,000× and 120,000× on the CCD, respectively. Focus was adjusted in a carbon film area adjacent to the recording position so that the CTF ring’s first node extends to the highest frequency, using the live computed Fourier transform (live FFT).

The ZPC-cryoEM images of epsilon15 bacteriophage were collected with a detector magnification of 75,000× with the same instrument as for bacteriorhodopsin crystal. A total of 6,188 particles were picked manually from 473 CCD frames using the EMAN boxer program (Ludtke et al., 1999). The same 6,188 particles were used for both icosahedral and asymmetric reconstructions with image sizes of 450×450 and 576×576, respectively.

Image processing of bacteriorhodopsin crystals

The 2D crystal images were processed by 2dx software (Gipson et al., 2007a; Gipson et al., 2007b). First we unbent the 2-D crystal images three times, using different peak mask sizes and reference areas. Based on the unbent images, we determined and corrected CTF for each image using the ctffind3 program implemented in the software. Three out of 15 images showing the lowest phase residuals were selected for generating a projection map. The Fourier components of the 3 images were merged by refining the phase origins. In the final projection map, P3 symmetry was imposed. The merged amplitudes from the images were compared with the electron diffraction amplitudes (Henderson et al., 1986) and plotted against the squared spatial frequency. The crystallographic B factor was estimated from the slope of the fitting line (Figure 1C).

Single particle reconstruction of epsilon15 bacteriophage with and without symmetry imposition

For icosahedral reconstruction, the alignment parameters of each particle image were determined by a Multi-Path Simulated Annealing optimization algorithm (MPSA) (Liu et al., 2007) based on cross common lines, and 3-D synthesis was performed using the EMAN make3d program (Ludtke et al., 1999). Using the MPSA procedure, a small number of particles were assigned random orientations, from which an initial model was built and then iteratively refined. Finally, without any CTF assessment, 4,558 good particles were selected using the consistency criterion of MPSA, resulting in a 10 Å resolution icosahedral map. For the asymmetric reconstruction, the asymmetric orientation was determined using cross common line between the tail image masked from a raw particle image and tail 2-D references projected from a 3-D tail model as described previously (Liu et al., 2010b).

To detect and remove high defocus particle images, which have their first CTF zero below 1/10 Å−1, we used a cross common line correlation curve between each raw image and 12 references projected from the 10 Å map. Each point of the resulting correlation curve is the 10 pixel running average of the cosine of the phase residual for that resolution range using all the cross common lines. In the correlation curve, high defocus images showing a negative correlation within 10 Å were discarded. The remaining 2,900 particle images were used to reconstruct the final 9 Å map. Resolution of the reconstruction was assessed according to 0.5 FSC between the two independently split data sets.

The structure factors of the final maps computed with or without symmetry imposition were rescaled to a synthetic 1-D structural factor that was generated through a procedure described in EMAN wiki (http://blake.bcm.tmc.edu/emanwiki/EMAN1/FAQ/StructureFactor) (Liu et al., 2010a). This structure factor rescaling has two functions: 1) boost up high frequency signal to make the high resolution features more visible; 2) boost up the very low frequency (lower than the cut-on frequency) signals that were suppressed due to the presence of the phase plate hole. The rescaled map was then low-pass filtered at a frequency slightly higher than the nominal resolution of the reconstruction.

Cryo-ET data collection and processing of epsilon15 bacteriophage

For ZPC-cryoET, tilt series of ice-embedded epsilon15 bacteriophage were collected with a Gatan 914 cryo-specimen holder at an angular increment of 2° with ±70° specimen tilt and at a detector magnification of ~30,000× on CCD. The total accumulated dose was ~80 electrons per Å2 using semi-automated low-dose tilt-series acquisition software (Danev et al., 2010).

Tomographic reconstruction was performed using IMOD software (Kremer et al., 1996). Fiducial gold markers (150 Å) included with the ice-embedded specimen were used for image alignment. Subtomograms of viral particles were extracted from the reconstructed tomogram. The alignment was done in two steps. First, the 20 Å low-pass filtered subtomograms, in which the particle tails were excluded by a tight spherical mask, were iteratively aligned to the total average in each step using tomohunter program (Schmid and Booth, 2008) after initially being aligned to a computer generated icosahedral model. Second, the tail density clearly visible at one of twelve 5-fold vertices was manually oriented to the Z-axis. The tail of epsilon 15 bacteriophage has pseudo 6-fold symmetry and is located at one of the 5-fold vertices. To find the unique azimuthal orientation of the tail from the five orientation choices, we masked the tail, applied 6-fold symmetry, and compared this symmetrized map with the single particle asymmetric map (Figure 3A) in 72º (5-fold) steps. The aligned subtomograms were averaged in reciprocal space by weighting the missing wedge to reduce orientation bias (Schmid and Booth, 2008). Resolutions of the asymmetric and icosahedrally averaged maps were assessed by comparing them with the higher-resolution single particle maps as described above, respectively.

Map visualization

The visualization was done by UCSF Chimera (Pettersen et al., 2004). Amira was used for segmentation (Visage Imaging, Inc., USA). The backbone model of gp7 (PDB: 3C5B) was used to evaluate the segmented densities in the icosahedral map reconstructed by single particle analysis.

Supplementary Material

01

Acknowledgments

We thank Drs. John & Elena Spudich at UTHSC for the bacteriorhodopsin, Dr. Greg Pintilie at MIT for segmenting the portal structure of epsilon15, Mr. Hiroshi Okawara at NIPS for preparing the phase plates. We also thank Dr. Kazutoshi Tani at Kyoto University for his technical assistance in the 2-D crystal image processing, Mr. Htet Khant at BCM for preparing the tomography sample, and Mr. Ryan Rochat at BCM for his critical reading of the manuscript. This work was supported by NIH grants (P41RR002250, R01AI0175208 and PN1EY016525), Robert Welch Foundation (Q1242) and a Core Research for Evolutional Science and Technology (CREST) of Japan Science and Technology Corporation to K.N.

Footnotes

AUTHOR CONTRIBUTIONS

J.K. provided epsilon15 sample. K.M. took all images with helps from J.J., R.D., K.N. and W.C.. X.L. developed cross-common-line correlation algorithm to determine the first CTF zero of a ZPC-cryoEM particle image and did the single particle reconstructions for the epsilon15 bacteriophage. K.M. processed both 2D crystal and tomographic data with M.F.S. assistance. X.L., K.M. and W.C. interpreted the maps. K.M., X.L., M.F.S. and W.C. wrote the paper with contributions from all co-authors.

ACCESSION NUMBERS

The density maps of epsilon15 bacteriophage obtained by single particle or tomographic reconstrucitons have been deposited to the EMDB under accession numbers EMD-5206 (9Å icosahedral reconstruction using 2900 ZPC-cryoEM particles), EMD-5207 (13Å asymmetric reconstruction using 5,600 ZPC-cryoEM particles), EMD-5208 (26Å asymmetric reconstruction using 100 ZPC-cryoEM particles), EMD-5209 (13Å asymmetric reconstruction using 17,800 conventional cryoEM particles), EMD-5203(32Å ZPC tomographic reconstruction averaged from 50 subtomograms without imposing symmetry) and EMD-5204 (38Å conventional tomographic reconstruction averaged from 50 subtomograms without imposing symmetry).

SUPPLEMENTAL INFORMATION

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