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Swarna makshika [SM], a mineral having various therapeutic uses, has been used since long in Ayurveda. The present study was conducted to generate a fingerprint for raw and processed SM using techniques which can be used by pharmacies. Powdered SM was heated in an iron pan by adding lemon juice for 3 days, till liberation of sulfur fumes stopped. Bhasma of this shuddha SM was obtained by triturating it withit with shuddha gandhaka and lemon juice. It was then subjected to heat in 09* putas, and for firing in each puta, 4 kg cow dung cakes were used. To assure the quality of bhasma, rasa shastra quality control tests like nischandratva, varitara, amla pariksha, etc., were used. After the bhasma complied with these tests, the bhasma was analyzed using X-ray Diffraction (XRD) analysis of raw SM and SM bhasma revealed that raw SM contains CuFeS2, and SM bhasma contains Fe2O3, FeS2, CuS and SiO2. Scanning Electron Microscope (SEM) studies showed that the grains in SM bhasma were uniformly arranged in agglomerates of size 1-2 microns as compared to the raw SM which showed a scattered arrangement of grains of size 6-8 microns. It may be concluded that raw SM is a complex compound which gets converted into a mixture of simple compounds having very small particle size after the particular process of marana. This is the first report of fingerprinting of SM bhasma prepared using this particular method.
Swarna makshika [SM] bhasma has been used for pandu (anemia), anidra (insomnia), apasmara (convulsions), mandagni (poor digestion), kustha (skin diseases), etc., as well as a potent rasayana drug. Generally, SM bhasma is prepared in two steps: shodhana, by different techniques like fomentation, heating and quenching and roasting, etc.; and marana, by puta system of heating in different types of putas, like varahaputa, kukkutaputa, gajaputa, etc.; and kupipakwa procedures, etc. During marana, bhavana with lemon juice, kulottha decoction, eranda taila, snuhi ksheera, etc., are given with addition of shuddha gandhaka and shuddha hingula, etc., as associated materials.
SM bhasma is used as a single constituent formulation or in multi-ingredient formulation. However, there is variation in collection of raw materials and the pharmaceutical procedure followed, which generates the same bhasma with different characters. As a result, reproducibility is often not achieved. In many cases, wrong manufacturing and marketing practice leads to the production of inferior quality products, which reduces efficacy or produces safety concerns. In order to minimize variability and to check adulteration, standardization of a bhasma is a must.
Ayurvedic texts have described methods for quality control of finished products through different parameters like nischasndratva, varitara, nirutha, apunarbhava, etc., to achieve a specific acceptable standard bhasma. This study was performed to characterize the bhasma using sensitive tools and techniques. These fingerprints generated for the raw material and bhasma could be used as standards to for ensuring quality and reproducibility of standards of the medicines.
This was done using standard procedures and included two steps, namely Shodhana and marana of Swarna makshika. The Swarna makshika was procured from the pharmacy (I.M.S., BHU) and lemon juice (sufficient quantity) was obtained from the market
Iron mortar and pestle, charcoal furnace, iron pan, iron ladle, pyrometer, etc.
At first the Swarna makshika was powdered in an iron mortar with an iron pestle. A clean and dry iron pan was then heated on a charcoal furnace onto which was poured the powdered Swarna makshika and subjected to intense heat with frequent addition of lemon juice till the liberation of sulfur fume stopped and it turned red. The process was completed in 3 days and the final product called Shodhita Swarna makshika obtained.
For the Marana of Swarna makshikashuddha gandhaka and lemon juice (q.s.) were procured. Equal amounts of shuddha Swarna makshika and shuddha gandhaka were triturated with lemon juice till a homogenous paste was formed. After triturating, small pellets of uniform size and thickness were prepared and dried in sunlight. Pellets were kept inside a sarava (shallow earthen disc) and another sarava was inverted over it. The joint between the two discs was sealed with a rag and mud/kapad mitti: a ribbon of fine cloth uniformly smeared with fuller's earth seven times and dried in sunlight.
The properly sealed and dried samputa was subjected to puta system of heating with 4 kg cow dung cake. The process was repeated using shuddha gandhaka in equal proportion to Swarna makshika for the first cycle and then in half the proportion for subsequent 8 cycles. Bhasma of the desired quality was obtained in 9 putas. The bhasma obtained from the above process was taken for analysis.
The final bhasma was analyzed for quality control as described in Ayurvedic texts as follows and found suitable:
The bhasma as well as the starting material (raw swarna makshik) was also analysed using the following techniques:
X-ray diffraction studies were performed in the Regional Research Laboratory (CSIR), Bhubaneswar.
The powdered sample was spread onto a double-side tape with a spatula, which was then placed on an aluminum sample holder. All the peaks were recorded on the chart, and the corresponding 2 theta values were calculated. Results are summarized in Figures Figures11 and and22 as well as Tables Tables11 and and22.
The strongest peak identified in the raw material was CuFeS2 while that in the final product was Ferrous oxide of Iron (Fe2O3). In the raw material, only one phase of Copper iron Sulphide (CuFeS2) was identified while in the final product, different phases were identified including Fe2O3, FeS2, Copper sulphide (CuS) and SiO2.
Scanning Electron Microscopy (SEM) was performed in the Dept. of Physics, BHU.
The mounted sample was placed inside the microscope's vacuum column through an airtight door, and then the air was pumped out. After the air was pumped out of the column, a beam of electrons was emitted by an electron gun from the top. This beam travels downward through a series of magnetic lenses designed to focus the electrons to a very fine spot. Near the bottom, a set of scanning coils made the focused beam to move back and forth across the mounted sample, row by row.
As the electron beam hits each spot on the sample, secondary electrons are backscattered from its surface. A detector counts these electrons and sends the signals to an amplifier. The final image was built up from the number of electrons emitted from each spot on the sample [Figures [Figures33 and and44].
The particles in the raw material and in the sample of intermediary process were not uniformly arranged while in the final product the particles uniformly arranged. In the final product, more agglomerates of grain were observed, whereas in the raw material the grains were found scattered. The size of the particles was less in the final product.
The particle size of the raw material was between 6 and 8 µ while that for the bhasma particles was 1-2 µ.
It is noteworthy that there are very specific pharmaceutical procedures and techniques described in the rasa shastra literature which convert the toxic metals/ minerals into a suitable dosage form. The bhasmas prepared are well tolerated both for short-term and long-term use; moreover, it is claimed that their prolonged administration is required to achieve the rejuvenation effect. According to the need of time, characterization of bhasma using scientific techniques is necessary to determine the effect of the process and to judge its safety and efficacy. SM bhasma was prepared and studied with this objective. X-ray diffraction study of the raw material showed a sharp peak, indicating its crystalline nature; whereas the final product did not give sharp peaks, indicating the loss of crystalline nature. This is suggested by the test described in Ayurveda namely, as loss of luster (nischandrika) in the final product. Thus the sharp crystalline structure of the raw material reflects light rays whereas loss of crystalline nature in the final product prevents it from doing so.Hence the "loss of luster" (nischandrika)described in Ayurveda as a quality to be looked for in the final product.
The study also revealed peaks of CuFeS2 in the raw material and Fe2O3, Iron sulphide (FeS2), CuS and silicon oxide (SiO2) in the final product. The formation of some different compounds in the final product may be due to oxidation and reduction reaction of Cu, Fe with sulfur in the presence of oxygen. It is likely that the lack of change of colour in an acidic medium (amla pariksha) in the final product is due to the absence of free metallic groups as free copper reacts with lemon juice to give a blue color. Sulfides and oxides of iron and copper present in makshika bhasma do not show any unwanted effect in experimental study, and the bhasma has been used over a long period of time in clinical practice and no toxic effect has been recorded so far. Presence of SiO2 may be due to the use of earthen casseroles, which may have a reaction with oxygen. Scanning electron microscopy study showed that the particle size reduced from 6-8 µ to 1-2 µ after bhasma process. It is this significant reduction of size and that allows the phenomenon of rekhapurna and varitara to develop. Reduction in particle size facilitates absorption and assimilation of the bhasma in the system. Again the clusters of particles are regular and uniform in the final bhasma in comparison to the raw material. The particle size recorded can be characterized as the desired specification of the final bhasma.
Thus, Swarna makshika which contains iron (Fe), Copper (Cu) and sulfur The manufacturing process plays a specific role to convert the CuFeS2 in the raw material mixture of Fe2O3, FeS2, CuS and SiO2, in the final product. These could be important chemical markers for SM bhasma prepared using this particular method. As a result of different stages of processing techniques like shodhana (which involves roasting, with addition of herbal juices and continuous stirring) and marana [which involves bhavana (wet trituration) and puta system of heating], the particle size reduces significantly, which may facilitate absorption and assimilation of the drug into the body system. The particle size in the final bhasma was 1-2 µ, which could be specified as the criterion for the final product conforming to all the traditional parameters under bhasma pariksha (examination of properly prepared bhasma). This can be one of the important factors for standardization of bhasmas.Thus, modern techniques can assist in proper characterization of Ayurvedic dosage forms and standardization of Ayurvedic medicines.
Varitara- The bhasma that floats on water is termed as varitara.
Apunarbhava- Bhasma when mixed with mitrapanchaka and heated at high temperature should not undergo any change in its physical properties. The bhasma should not regain its original state.
Niruttha- Bhasma is heated at high temperature in a koshthi along with measured quantity of silver. At the end of the process, the quantity of silver should not increase.
Niswadu- Bhasma should be tasteless. If bhasma has any taste, it is considered as semi-finished and should be subjected to puta again.
Nischandra- The sparkling particles (chandrika) in a bhasma indicate a semi-finished product.
Avami- The bhasma should not produce nausea on administration.
Puta- In continuation with the etymological meaning, puta is the measure of the amount of heat required to convert or transform any metal or mineral. This amount is substance specific and measured in terms of number or weight of fuel.
Sharava- Earthen Petri dish having specific measurements.
Bhavana- Trituration of the drug with liquid medium, e.g., hingula with juice of fresh zinzibar officinalis.
CCRAS, New Delhi for providing financial assistance, Prof. O. N. Srivastav (former Professor, Department of Physics, BHU for providing facilities for SEM), Dr. Devanada, Reader, Department of Physics, BHU, providing Co operation for SEM, Dr. P. S. Mukharji, Scientist, Regional Research laboratory, Bhubanesware for co operation in X-RD studies
Source of Support: CCRAS, New Delhi
Conflict of Interest: None declared.