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F1000 Biol Rep. 2009; 1: 31.
Published online 2009 April 29. doi:  10.3410/B1-31
PMCID: PMC2924701

Long-distance transport of macromolecules through the phloem

Abstract

Long-distance phloem transport of small metabolites has long been the subject of many different studies concentrating on resource allocation and signalling between plant organs. Also, phloem movement of viruses has long been examined as the route for systemic infection of the plant. Only recently, the transport of macromolecules, such as proteins and nucleic acids, has received increasing attention because they are regarded as being a new class of potential information-transmitter. A set of recent publications allows the first insights into the important roles that phloem-mobile macromolecules might play in the regulation of development and the responses to stress. Furthermore, they start to shed light on the mechanisms involved in systemic macromolecule transport.

Introduction and context

Cell-to-cell and interorgan exchange of nutrients and information is essential for growth, development, successful reproduction, and environmental adaptation of multicellular organisms. Higher land plants have evolved a plant-specific symplastic network connected via plasmodesmata (PD), cytoplasmic connections between plant cells, which allow a locally restricted exchange of information. In addition, a rapid transfer of information over longer distances is enabled by the phloem. PD and the phloem thus provide a continuous symplastic connection between even the most distant plant organs.

The phloem of higher land plants is a channel system mainly responsible for the allocation of organic nutrients that are produced during photosynthesis (photoassimilates) from ‘source’ organs (those with a net production of photoassimilates, such as green leaves) to ‘sink’ organs (those with a net consumption, such as roots or fruits). Together with the xylem (dead tubes that transport water from root to shoot), the phloem is located in vascular bundles. The transport tubes of the phloem, the so-called sieve elements (SEs), are living but partially degenerated cells that lose important cellular components such as vacuoles, the nucleus, or ribosomes during maturation. As a consequence, SEs have presumably lost the capacity for transcription and translation, resulting in their inability to synthesise RNAs or proteins. The specific maturation and their interconnection with other adjacent SEs make these tubes suited for efficient and quick substance transport, but hardly viable. For survival, SEs are therefore always intimately associated with their neighbouring companion cells (CCs), and both emerge from the division of a single mother cell. CCs remain fully equipped and develop high metabolic activity in order to provide SEs with all the vitally important compounds. To allow a steady transfer of molecules of different molecular classes and sizes, CCs and SEs are connected by special PD, namely the pore plasmodesmal units, which are formed by multiple channels on the CC side that lead into a single pore on the SE side. As a result, the size of molecules allowed to pass these PD, the so-called size exclusion limit (SEL), is quite high (>67 kDa) as compared with that of other PD (1 kDa) [1]. In addition, the capacity of many phloem polypeptide compounds to further increase the SEL [2] should, in principle, also enable macromolecules to pass through. It is well known that many plant viruses (macromolecular structures consisting of nucleic acids and proteins) exploit this symplastic pathway to spread from cell to cell and systemically through the entire plant. It was only recently discovered that a high number of plant endogenous macromolecules, such as proteins and RNAs, are also present within the phloem, which suggested that they might act as information-transmitting molecules in a diverse range of physiological processes. However, only recent advances have confirmed that some of these endogenous macromolecules can indeed be transported in living plants to influence developmental processes, as well as stress and defence responses. In addition, advances mainly from plant virus research allow new insights into the mechanisms that underlie the systemic translocation of macromolecules.

Major recent advances

Phloem transport of viruses

Long-distance transport of macromolecules was first seen by observing the spread of viruses that misuse the phloem for their passive translocation through the plant, leading to the spread of the infection, especially into growing areas. After infection, viruses normally spread locally from cell to cell through PD until they reach the phloem. Viruses contain special proteins, namely movement proteins (MPs), that can bind and unfold single-stranded RNAs and facilitate their intercellular translocation by building protein-RNA transport complexes [ribonucleoprotein (RNP) complexes] [3]. It is currently unknown whether MPs are also needed for phloem-dependent movement [4]. However, several studies indicate that cell-to-cell and systemic virus transport follow different mechanisms [5-7], and there seem to be different viral and endogenous plant components involved [7-10]. In addition to MP, an important viral factor for phloem movement is the coat protein that seems to be strictly required for long-distance translocation of several groups of viruses [11,12].

With regard to endogenous plant factors, a cell wall glycine-rich protein and a pectin methylesterase have been shown to influence viral spread, probably by modulating the permeability of PD [5,7,13]. An interesting recent finding is that the interaction of the groundnut rosette virus ORF3 protein with subnuclear Cajal bodies and the nucleolus is required to capture the plant protein fibrillarin to form an RNP complex capable of long-distance phloem movement [14,15]. Also, endogenous phloem proteins, namely different phloem lectins and the phloem protein CmPP16 (a protein with properties resembling those of virus MPs), have been found to interact with viral RNAs and endogenous mRNAs and thus have been suggested to be involved in virus import and/or translocation as RNP complexes [16,17], but a conclusive demonstration of their importance for viral spread is, as yet, missing.

Phloem transport of endogenous macromolecules

The occurrence of unspecified RNA species was described nearly 40 years ago, but it was long regarded as an artefact caused by sampling. The first evidence that endogenous plant mRNAs are present in SEs came from the in situ localisation of the mRNA of a sucrose transporter in SEs and the PD connecting SEs to CCs [18]. Meanwhile, a range of different mRNAs from phloem samples of various species, obtained using different sampling techniques, have been detected (reviewed in [19]) and they are nowadays regarded as being authentic components of the phloem stream. At the same time, heterografting studies performed by different research groups could show that specific transcripts can indeed move and, moreover, induce phenotypic alterations in their target tissues [20-22]. For example, in potato, overexpression of the transcription factor BEL1 mRNA induced a marked increase of tubers per plant, and this phenomenon could cross graft junctions, thus indicating phloem mobility of this mRNA [23].

Recently, small RNAs of fewer than 30 nucleotides [short interfering RNAs (siRNAs) and microRNAs (miRNAs)] were found to occur in the phloem stream [24-26]. siRNAs are involved in a process called post-transcriptional gene silencing, an innate plant defence mechanism against transposable elements and viruses [27] that can spread systemically throughout the plant [28]. While siRNAs are generally assumed to be mobile between cells and also systemic [29], most miRNAs, involved mainly in controlling developmental processes, seem to act cell-autonomously under normal growth conditions in adult tissues, as demonstrated, for example, by miR171 [30,31]. However, it was recently shown that specific nutrient starvation-responsive miRNAs (such as miR395 that responds to sulphur, miR398 to copper, and miR399 to phosphate stress) can accumulate in the phloem at high levels when plants are grown under the respective nutrient-deprived condition [26]. Moreover, different results suggested that during phosphate deprivation systemic signalling is required [32,33], and recently miR399 was shown to be indeed translocated across graft junctions in the model species Arabidopsis, indicating that phloem movement of this specific miRNA could be involved in regulating the response toward phosphate starvation systemically [34,35]. This observation, however, does not answer the question of whether miRNAs are generally mobile or immobile from cell to cell and over long distances. There might exist a small subset of specific mobile miRNAs, or miRNA mobility might be restricted to particular developmental stages and/or specific environmental conditions.

Also, the occurrence of proteins in phloem sap was noticed a long time ago and since then different studies that comprehensively identified phloem proteins from various species have been published [36-38]. However, the capability of the polypeptides to be transported in vivo has been demonstrated for only a few of them (for example, the pumpkin phloem protein CmPP16 that, as mentioned above, displays similarities to viral MPs) [39]. Moreover, the physiological functions of most of the phloem polypeptides remain unknown. For many of them, roles in signal transduction and stress responses have been suggested but not conclusively demonstrated. For several years, the Solanaceae-specific peptide systemin served as the paradigm for a phloem-mobile protein involved in long-distance signalling [40], but according to more recent results, it seems rather likely that it is jasmonic acid and not systemin that constitutes the phloem-translocated signal during wound responses [41]. To date, the only convincing example of a phloem protein involved in signalling is the flower-promoting protein flowering locus T (FT), which was recently shown to be present in the phloem at detectable concentrations when plants were sampled at the onset of flowering [38]. Subsequently, several independent studies have provided compelling evidence that FT long-distance transport can indeed contribute to flower induction in different plant species [42-46], and the FT protein could thus be a component of the long-sought ‘florigen’ [47]. Further studies are required to find out whether any of the other phloem sap proteins can act in the information transfer during other developmental processes or stress responses.

Although the transport mechanisms of endogenous macromolecules are not well understood, it is assumed that phloem import and transport of both proteins and RNAs are mediated by specific binding proteins acting as molecular chaperones. It was proposed that phloem heat shock proteins might participate in delivering polypeptides to the phloem [48,49], but other components with functions related to protein folding (for example, the abundant phloem cyclophilins [38]) could also be involved. Several phloem-sap proteins have also been suggested to transport RNAs in the form of RNP complexes since they are RNA-binding, are translocatable across graft unions, and can increase the SEL of PD [17]. In particular, the pumpkin phloem sap protein CmPP16 possesses properties similar to those of viral MPs and mediates the transport of its own and foreign mRNAs from cell to cell and over long distances through the phloem, as shown by microinjection studies and grafting experiments [39]. Small RNA transport in pumpkin seems to follow a similar route mediated by the small RNA-binding phloem protein CmPSRP1 that, however, seems to be restricted to this one plant species [24]. In other plants, one or more of the various RNA-binding phloem proteins that have been described to occur in phloem sap [16,36,38] could possibly do the job. Also, the obvious similarities between the translocation of viruses and endogenous macromolecules suggest that the underlying mechanisms are the same or at least highly similar, but both will need to be demonstrated in future experiments.

Future directions

The results from recent publications enhance our understanding of why, when, and how viruses and endogenous macromolecules can travel long distances through the phloem. On one hand, they have begun to shed light on the complex mechanisms of the long-observed systemic spread of viruses within plants. On the other hand, they have established that specific endogenous phloem proteins and RNAs can be transported long distances in vivo following certain triggers. While the translocation of viruses clearly serves to spread infection, the transport of endogenous macromolecules might contribute to communicate essential information during developmental processes or stress responses as, for example, for the flower-promoting FT protein or the phosphate-deprivation-induced miR399. Whether this holds true for only a small subset of endogenous macromolecules, or whether there are much more important physiological decisions influenced by the wide range of molecules found in the phloem, requires further investigation. To this end, it will be interesting to comprehensively identify the (sub)set of endogenous macromolecules that can really move long distances in vivo and to analyse the direction of movement and the triggers (developmental and environmental) that initiate transport. It will also be important to identify additional factors and interaction partners required for the movement of different viruses and endogenous macromolecules in order to establish the prerequisites for phloem mobility and import and transport selectivity. This knowledge will be the basis for creating plants with, for example, increased virus or stress resistance, or for influencing important developmental processes by effecting which macromolecules can ride on the ‘phloem information highway’.

Abbreviations

CC
companion cell
FT
flowering locus T
miRNA
microRNA
MP
movement protein
PD
plasmodesmata
RNP
ribonucleoprotein
SE
sieve element
SEL
size exclusion limit
siRNA
short interfering RNA

Notes

The electronic version of this article is the complete one and can be found at: http://F1000.com/Reports/Biology/content/1/31

Notes

Competing interests

The author declares that she has no competing interests.

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