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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Arthritis Rheum. Author manuscript; available in PMC Aug 1, 2011.
Published in final edited form as:
PMCID: PMC2922001
NIHMSID: NIHMS207537
Generalized Bone Loss as a Predictor of 3-Year Radiographic Damage in African American Patients with Recent-Onset Rheumatoid Arthritis
Jie Zhang, MPH,1 David T. Redden, PhD,1 Gerald McGwin, Jr, PhD, MS,1 Leigh F. Callahan, PhD,2 Edwin A. Smith, MD,3 Graciela S. Alarcón, MD, MPH,1 Larry W. Moreland, MD,1,4 Désirée M. van der Heijde, MD, PhD,5 Elizabeth E. Brown, PhD MPH, MSPH,1 Donna K. Arnett, PhD,1 Ted R. Mikuls, MD, MsPH,6 and S. Louis Bridges, Jr, MD, PhD1, for the CLEAR Investigators7
1University of Alabama at Birmingham, Birmingham, AL, USA
2University of North Carolina, Chapel Hill, NC, USA
3Medical University of South Carolina, Charleston, SC, USA
5Leiden University Medical Center, Leiden, The Netherlands
6University of Nebraska Medical Center, Omaha, Nebraska, USA
Address correspondence and reprint request to Jie Zhang, MPH (Corresponding Author), Department of Epidemiology, School of Public Health, University of Alabama at Birmingham 1665 University Blvd, Birmingham, AL 35294. jszhang/at/uab.edu, Tel: 205-222-6275, Fax: 205-975-2541
4Current address: University of Pittsburgh, Pittsburgh, PA, USA
7The CLEAR Investigators are: George Howard, DrPH (University of Alabama at Birmingham, Birmingham, AL); Doyt L. Conn, MD (Emory University, Atlanta, GA); Beth L. Jonas, MD and Richard D. Brasington, Jr., MD (Washington University, St. Louis, MO).
Objective
To examine the association between baseline bone mineral density (BMD) and radiographic damage at 3-year disease duration in a longitudinal cohort of African Americans (AAs) with recent-onset RA.
Methods
Participants (n=141) included AAs with < 2 years of disease duration. All patients underwent baseline BMD measurement (femoral neck and/or lumbar spine) using DXA. T-scores were calculated using AAs normative data. Patients were categorized as having osteopenia/osteoporosis (T score ≤ −1) or healthy. Hand/wrist radiographs, obtained at baseline and at 3-year disease duration, were scored using modified Sharp/van der Heijde method. The association between baseline BMD and total radiographic score at 3-year disease duration was examined using multivariable negative binomial regression.
Results
At baseline, the mean age and disease duration were 52.4 years and 14.8 months respectively (85.1% women). Average total radiographic scores at baseline and 3-year disease duration were 2.4 and 5.7. In the final reduced multivariable model adjusting for age, gender, anti-cyclic citrullinated peptide antibody positivity, and the presence of radiographic damage at baseline, the total radiographic score at 3-years of disease duration in patients with osteopenia/osteoporosis at the femoral neck was twice that in patients with healthy bone density and the difference was statistically significant (p=0.0084). No association between lumbar spine osteopenia/osteoporosis and radiographic score was found.
Conclusion
These findings suggest that reduced generalized BMD may be a predictor of future radiographic damage and support the hypothesis that radiographic damage and reduced generalized BMD in RA patients may share a common pathogenic mechanism.
The progression of radiographic damage in rheumatoid arthritis (RA) is not uniform (1) and despite the advent of biologic agents, RA continues to cause joint destruction in a significant proportion of patients (26). Initial radiographic damage, antibodies to cyclic citrullinated peptide (anti-CCP), C-reactive protein (CRP) levels, and other inflammatory markers are currently used to identify patients at greatest risk for developing progressive joint damage in an effort to selectively implement early, aggressive treatment strategies (711).
Recent findings among RA patients of European ancestry have suggested that lower bone mineral density (BMD) at various anatomic sites can be used as a predictor of progressive radiographic damage. Significant associations between hand BMD and subsequent radiographic damage in patients with early RA have been reported independently in three longitudinal studies (1214). Forslind et al showed that generalized bone loss was a significant predictor of radiographic progression at 2-year follow-up (15). In addition to these longitudinal studies, generalized BMD has been shown to be significantly associated with radiographic damage or joint erosions in a number of cross-sectional studies (1619).
However, it is unknown whether these results can be extrapolated to African American RA patients. Healthy African Americans have higher BMD (2022) and a significantly lower risk of osteoporosis-related fractures than Caucasians, Asians, and Hispanics (23). African Americans not only achieve a higher peak bone mass but differ from European Americans with respect to skeletal resistance to parathyroid hormone, estradiol and testosterone levels, calcium metabolism, and the rate of bone turnover (2429).
The objective of this study is to examine whether baseline generalized BMD at the femoral neck and lumbar spine correlates with radiographic joint damage accrued over time in a longitudinal cohort of African American patients with recent-onset RA.
The analyses were performed in participants enrolled in the Consortium for the Longitudinal Evaluation of African Americans with Early RA (CLEAR) Registry from 2001 to 2005. The CLEAR Registry has four sites participating in this longitudinal study, including the University of Alabama at Birmingham (Coordinating Site), Emory University, the University of North Carolina, and the Medical University of South Carolina(30). The study was approved by the Institutional Review Board (IRB) at each participating site and all participants provided informed written consent.
Briefly, subjects were eligible if their self-reported race was black or African American, if they had a diagnosis of RA as defined by the American College of Rheumatology (ACR) 1987 classification criteria (31), and if the disease duration was less than 2 years from symptom onset. At the baseline visit, questionnaires were administered to collect information on medical history (ACR criteria, extra-articular manifestations, disease duration, current and past use of RA and non-RA medications, co-morbidities) and demographics. All patients underwent a physical examination during which information on height and weight was obtained. The number of tender and/or swollen joints was assessed by trained evaluators; the Joint Alignment and Motion scale was used to assess joint deformity (32); and the Health Assessment Questionnaire (HAQ) Disability Index was used to evaluate functional ability (33). Serum rheumatoid factor (RF-IgM, IU/ml, INOVA Diagnostics, USA) and anti-CCP antibodies (U/ml, Diastat, Axis-Shield Diagnostics Ltd., UK) were measured, as previously reported (34). Plasma high-sensitivity C-reactive protein (hs-CRP, mg/L) concentrations were determined using an immunoturbidimetric assay on the Hitachi 917 analyzer (Roche Diagnostics - Indianapolis, IN), using reagents and calibrators from DiaSorin (Stillwater, MN).
Participants were re-evaluated at 3-years disease duration (time elapsed since symptom onset). Because the CLEAR participants were enrolled at any point less than 2 years after disease onset, the disease duration at baseline visit varied from 0–2 years. As a result, the amount of follow-up time also varied among study subjects. Between the baseline and 3-year visits, patients were surveyed via telephone at 6-month intervals to obtain information about medication use since the last study encounter.
The outcome variable of our study was radiographic damage at the 3-year follow-up visit. Radiographs of the hands and wrists were obtained at baseline and at the 3-year visits and radiographic damage was evaluated using the van der Heijde modified Sharp (SvdH) method (35). Baseline BMD at the femoral neck and lumbar spine were measured at each participating center using dual energy x-ray absorptiometry (DXA). BMD measures were standardized to Hologic BMD using published conversion equation (36) and T-scores were calculated using referent data from the general African American population: manufacturer’s reference database (lumbar spine) and National Health and Nutrition Examination survey-III data (femoral neck). Details with regard to the collection, standardization, and calculation of T-scores have been previously published (37). Based on baseline T scores, patients were categorized into two groups for each anatomical site: “Osteopenia/Osteoporosis” (T-score less than or equal to −1) or “Healthy” (T-score greater than −1).
Statistical Analyses
Patients in the CLEAR Registry with SvdH score at the 3-year visit and baseline BMD at either femoral neck or lumbar spine were included in the analysis (n=141). The dependent variable was the total SvdH score at the 3-year visit, which was a non-negative integer assuming an over-dispersed Poisson distribution (variance greater than mean). Hence, linear models were not appropriate and negative binomial regressions were used in both univariate and multivariable analyses. The exponentiation of parameter estimate (EPE) of the negative binomial regression model can be interpreted in a multiplicative manner such as in the following example, e.g., an EPE of 3 for female gender indicates that the total SvdH score for a female is 3 times that for a male if the two subjects were the same with regard to all other covariates. An EPE greater than 1 indicates that the presence of the characteristic (or a higher value in the case of a continuous variable, e.g., older age) is associated with greater total 3-year SvdH score and an EPE less than 1 indicate the opposite.
A wide range of baseline characteristics were examined in univariate analyses, including age, gender, body mass index (BMI), disease duration at baseline, baseline plasma hs-CRP level (mg/L), anti-CCP antibody positivity, RF-IgM positivity, presence of radiographic damage (SvdH score > 0), HLA-DRB1 shared epitope (SE) positivity (the HLA-DRB1 alleles encoding the SE were: HLA-DRB1*0101, *0102, *0401, *0404, *0405, *0408, *0413, *1001, and *1402), cumulative oral glucocorticoid use (a composite of dose, frequency, and duration) prior to the 3-year visit was categorized approximately into 3 tertiles: never (n=47), low (n=43), and high (n=44), HAQ score, tender and swollen joint counts, number of ACR criteria, number of extra-articular manifestations, and smoking status (never, past, or current smoker).
Age, gender, BMI, cumulative glucocorticoid use, smoking status, and variables that had p-values less than 0.10 in univariate analyses were included in an initial multivariable model. To produce a final parsimonious model, the initial model was then manually reduced by removing variables that had the greatest p-value at each step if they were not statistically significant at 0.05 significance level and if the removal of the variables did not produce a 10% or greater change in the parameter estimate for femoral neck or lumbar spine osteopenia/osteoporosis (38). Because BMD at femoral neck and lumbar spine were highly correlated, two separate multivariable models were examined for BMD at the two anatomical sites.
All statistical analyses were performed using SAS statistical package (version 9.1, SAS Institute Inc., Cary, North Carolina).
Baseline patient characteristics are shown in Table 1. At the baseline visit, the mean (SD) age and disease duration were 52.4 (13.0) years and 14.8 (7.3) months, respectively. Women accounted for 85.1% of the total study population. Mean (SD) SvdH scores at baseline and 3-year visits were 2.4 (5.4) and 5.7 (11.3), respectively, with an average annual progression rate of 1.5 units.
Table 1
Table 1
Baseline characteristics of African Americans with recent-onset rheumatoid arthritis (n=141)
At baseline, the average BMD (g/cm2) at lumbar spine and femoral neck were 1.08 and 0.85. At lumbar spine 38.6% of the subjects had either osteopenia or osteoporosis and at femoral neck 44.9% (T-score ≤ −1). Patients with osteopenia/osteoporosis at either anatomical site had higher mean total SvdH scores at baseline and more progression during the follow-up period. At femoral neck, the mean total SvdH scores at baseline and 3-year visits in patients with osteopenia/osteoporosis were 3.31 and 8.16 respectively and in patients who had healthy bone density the means were 1.76 and 3.91. At lumbar spine, the mean total SvdH scores at baseline and 3-year visits were 3.10 and 7.63 in patients with osteopenia/osteoporosis and were 2.00 and 4.58 in patients who had healthy bone density.
Table 2 presents the results of the univariate analyses and the final reduced multivariable model. Femoral neck osteopenia/osteoporosis at baseline was associated with higher total SvdH score at the 3-year visit (EPE=2.2; p=0.0249). After controlling for other baseline characteristics, femoral neck osteopenia/osteoporosis remained significantly associated with higher total SvdH score in the multivariable analyses (EPE=2.1; p=0.0084). The removal of age and glucocorticoid use from the multivariable model resulted in a 17% increase and a 21% reduction respectively in the parameter estimate for femoral neck osteopenia/osteoporosis, which exceeded the 10% cut-off point used in the “change in estimate” strategy to evaluate confounding (38). While they were not statistically significant at 0.05 alpha level, they were kept in the final multivariable model (Table 2). To test the robustness of our final model, we built an alternate reduced model retaining only those variables that are significant at 0.05 alpha level and the results were similar. The 3-year total SvdH scores in subjects with reduced bone density at the femoral neck were twice those of subjects with healthy bone density (EPE=2.1; p=0.0035).
Table 2
Table 2
Univariate and multivariable models between baseline characteristics and 3-year van der Heijde modified Sharp score among African Americans with recent-onset rheumatoid arthritis (n=141)
Baseline osteopenia/osteoporosis at the lumbar spine was marginally associated with the SvdH score (EPE=1.8; p=0.0897) in the univariate analysis. There was no final reduced multivariable model for lumbar spine BMD. Lumbar spine osteopenia/osteoporosis was not significantly associated with 3-year total SvdH in the full model and was eliminated during variable elimination.
We excluded two subjects whose 3-year SvdH scores were five standard deviations (SDs) above the mean and were osteoporotic at baseline in order to evaluate the sensitivity of the final multivariable model. Reduced bone density at femoral neck remained a significant predictor of total SvdH score (EPE=2.1; p-value = 0.0093).
Given that generalized BMD may be more strongly related to bony erosions than to joint space narrowing or total SvdH scores, we performed additional analyses examining the relationship between femoral neck and hip osteopenia/osteoporosis with erosion scores at 3-years disease duration, using the same method described above. Femoral neck osteopenia/osteoporosis was significantly associated with the 3-year disease duration erosion score in univariate (EPE=2.2; p=0.0279) and multivariable analyses (full model EPE=2.7; p=0.0061). A marginally significant association was observed for lumbar spine osteopenia/osteoporosis in univariate (EPE=2.0; p=0.0535) and multivariable analyses (full model EPE=1.8; p=0.0594). These results are summarized in table 3.
Table 3
Table 3
Univariate and multivariable models between baseline characteristics and 3-year erosion score among African Americans with recent-onset rheumatoid arthritis (n=141)
RA patients are at increased risk of developing osteoporosis compared with those without RA (39). Increasing evidence suggests a common mechanism for generalized bone loss and localized radiographic joint damage. A number of cross sectional studies have reported a significant correlation between the two types of bone manifestations(1619) and agents targeting tumor necrosis factor (anti-TNF treatment) has been found to halt generalized bone loss in RA patients in addition to its effect on localized joint damage (40). The mechanism has been postulated to involve inflammatory cytokines in the regulation of osteoclast differentiation and activation. Research interest has focused on the RANK/RANKL (receptor activator of NF-κβ/RANK ligand) pathway and TNF-α (41, 42). Our findings of significant associations between reduced BMD (osteopenia/osteoporosis) at femoral neck with 3-year total SvdH score and the erosion score in African American patients with recent-onset RA lends further support to this hypothesis.
Our results raises the question of whether bone density is already reduced in early RA patients compared with general population. We had obtained bone mineral density data on approximately frequency age- and gender-matched African American controls (n=161). Using multivariable linear regression to further control for age and gender, there was a marginally significant difference for femoral neck BMD (p=0.0767). Given the same age and gender, the bone density in RA patient was 0.03 point higher than that in a control. This further suggests that the balance in bone remodeling is already tilted by the disease.
Our findings also have potentially important clinical implications in the treatment of patients with recent-onset RA by suggesting that generalized BMD may be used as a predictor of subsequent radiographic damage. The identification of early RA patients with more a aggressive disease course is of great importance, particularly since prompt initiation of disease modifying treatments have been shown to protect against joint damage and RA-related functional and work disability (43, 44). Denosumab, an investigational human monoclonal antibody against RANKL, has been shown to reduce localized joint erosions in RA patients and to increase bone density in postmenopausal women (45, 46). Perhaps, given the ability of the new agent to treat both types of bone manifestations, reduced BMD can be used as more than a biomarker to predict future joint damage, but also as a biomarker to identify patients who are likely to benefit most from such new therapy and as an indication to initiate the treatment.
We did not find a significant association between baseline lumbar spine osteopenia/osteoporosis with 3-year SvdH score. The discrepancy that an association was observed between radiographic joint damage with BMD at the hip but not at the lumbar spine has been reported previously in patients of European ancestry (1618). One likely explanation for the discrepancy is osteoarthritis and/or other vertebral deformities occurring commonly in elderly population. Osteophyte formation, bone sclerosis, and disk space narrowing have been found to correlate positively with lumbar spine BMD whereas such a correlation was either not observed or observed to a lesser degree with hip BMD (47). The issue that lumbar spine BMD is often falsely inflated is reflected in the clinical guidelines of the National Osteoporosis Foundation (NOF), which recommends hip BMD as the preferred site for the diagnosis of osteoporosis(48).
In our study, peak referent BMD from African Americans was used to derive the T-scores. Because the objective of the study was not to assess fracture risk but to evaluate the association between radiographic damage and reduced bone mass, the use of race-specific referent data allowed us to determine the severity of bone loss and to measure the amount of reduction from the expected peak BMD. We replicated the same models using BMD as a continuous variable. Without adjusting for other variables, femoral neck BMD was marginally associated with the 3-year total SvdH score (EPE=0.21, p=0.0765) and lumbar spine BMD was not (EPE=0.45, p=0.3239). Adjusting for the same covariates as in the final reduced model, femoral neck BMD was again borderline significant at p-value 0.0542 (EPE=0.14). While the association did not reach the 0.05 threshold, the results were consistent with those when the dichotomized measures were used.
There are a number of limitations in our study. First and foremost, the relatively small sample size (n=141) is likely to have resulted in the exclusion of a number of baseline characteristics from entering the multivariable analyses. To address this issue, the change-in-estimate approach has been used to evaluate possible confounders and to fit the final multivariable model. Perhaps more importantly, because under-powered early studies tend to report over-inflated effect size (49), the magnitude of the association found in our study will need to be evaluated and interpreted with caution.
Another limitation of our study concerns the accuracy of the patients’ self-reported cumulative oral glucocorticoid use. Patients were asked to provide detailed information on the dose, duration, and type of glucocorticoid used and were then categorized into tertiles of cumulative glucocorticoid use. While previous research has shown robust agreement between quartiles of cumulative glucocorticoid dose from self-report and medical records review (50), it is possible that the self-reported information was inaccurate and patients were misclassified into the wrong group regarding this important exposure. Misclassification may result in uncontrolled confounding and inflation of the magnitude of the association between generalized BMD and radiographic damage we found in our study.
The association between generalized BMD and radiographic damage may be confounded by multiple factors. In addition to glucocorticoid use, physical inactivity due to impaired function, body mass index (BMI), disease activity, and medications may all influence the relationship. A study by Solomon et al. (16) found that the relationship between focal bone erosions and generalized BMD was stronger among patients with shorter disease duration and lower cumulative oral glucocorticoid use, suggesting that in patients with longer disease duration, the other factors exerted greater influence on the relationship between bony erosions and generalized BMD.
Despite the limitations discussed above, our study is the first to examine the relationship between generalized BMD and radiographic damage in African American patients with recent-onset RA. African Americans are under-represented in RA research and the known racial/ethnic differences in the skeletal system render it unclear whether the previously reported results apply in this population.
In summary, the findings from our study suggest that generalized bone density may be a predictor of radiographic damage and provide further support for the hypothesis that the two types of bone manifestations share a common mechanism. The elucidation of such mechanism responsible for the imbalance in bone remodeling in future research will not only lead to a better understanding of the pathogenesis of RA but also one more step toward individualized medicine and development of novel treatment therapies that have the potential of addressing both types of bone involvement.
Acknowledgments
We gratefully acknowledge staff and coordinators at the following sites: University of Alabama at Birmingham: Stephanie Ledbetter, MS; Selena Luckett, RN, CRNC; Laticia Woodruff, RN, MSN; Candice Miller; Emory University: Joyce Carlone, RN, RNP; Karla Caylor, BSN, RN; Sharon Henderson, RN; University of North Carolina: Diane Bresch, RN; Medical University of South Carolina: Trisha Sturgill. Washington University: Teresa Arb, RN.
Supported by NIH contract N01 AR-02247 and GCRC Grant M01 RR 00032 (University of Alabama at Birmingham); Emory University/Grady Hospital: M01 RR 00039, University of North Carolina: M01 RR 00046, Medical University of South Carolina: M01 RR 01070; NIH N01-AR-6-2278 (Continuation of the Consortium for the Longitudinal Evaluation of African-Americans with Early Rheumatoid Arthritis (CLEAR) Registry); and the Participant & Clinical 2 Interactions Resources component of the UAB Center for Clinical and Translational Science of NIH grant UL1 RR025777
Dr. van der Heijde has received research grants and/or consulting fees from Abbott, Amgen, Centocor, Novartis, Roche, Schering-Plough, Wyeth, and UCB.
1. Graudal NA, Jurik AG, de Carvalho A, Graudal HK. Radiographic progression in rheumatoid arthritis: a long-term prospective study of 109 patients. Arthritis Rheum. 1998;41(8):1470–1480. [PubMed]
2. Goekoop-Ruiterman YP, de Vries-Bouwstra JK, Allaart CF, van Zeben D, Kerstens PJ, Hazes JM, et al. Clinical and radiographic outcomes of four different treatment strategies in patients with early rheumatoid arthritis (the BeSt study): A randomized, controlled trial. Arthritis Rheum. 2008;58(2 Suppl):S126–S135. [PubMed]
3. van der Heijde D, Klareskog L, Landewe R, Bruyn GA, Cantagrel A, Durez P, et al. Disease remission and sustained halting of radiographic progression with combination etanercept and methotrexate in patients with rheumatoid arthritis. Arthritis Rheum. 2007;56(12):3928–3939. [PubMed]
4. Genovese MC, Bathon JM, Fleischmann RM, Moreland LW, Martin RW, Whitmore JB, et al. Longterm safety, efficacy, and radiographic outcome with etanercept treatment in patients with early rheumatoid arthritis. J Rheumatol. 2005;32(7):1232–1242. [PubMed]
5. Breedveld FC, Emery P, Keystone E, Patel K, Furst DE, Kalden JR, et al. Infliximab in active early rheumatoid arthritis. Ann Rheum Dis. 2004;63(2):149–155. [PMC free article] [PubMed]
6. Genta MS, Kardes H, Gabay C. Clinical evaluation of a cohort of patients with rheumatoid arthritis treated with anti-TNF-alpha in the community. Joint Bone Spine. 2006;73(1):51–56. [PubMed]
7. Jansen LM, van Schaardenburg D, van der Horst-Bruinsma I, van der Stadt RJ, de Koning MH, Dijkmans BA. The predictive value of anti-cyclic citrullinated peptide antibodies in early arthritis. J Rheumatol. 2003;30(8):1691–1695. [PubMed]
8. Drossaers-Bakker KW, Zwinderman AH, Vliet Vlieland TP, Van Zeben D, Vos K, Breedveld FC, et al. Long-term outcome in rheumatoid arthritis: a simple algorithm of baseline parameters can predict radiographic damage, disability, and disease course at 12-year followup. Arthritis Rheum. 2002;47(4):383–390. [PubMed]
9. Bukhari M, Lunt M, Harrison BJ, Scott DG, Symmons DP, Silman AJ. Rheumatoid factor is the major predictor of increasing severity of radiographic erosions in rheumatoid arthritis: results from the Norfolk Arthritis Register Study, a large inception cohort. Arthritis Rheum. 2002;46(4):906–912. [PubMed]
10. Combe B, Dougados M, Goupille P, Cantagrel A, Eliaou JF, Sibilia J, et al. Prognostic factors for radiographic damage in early rheumatoid arthritis: a multiparameter prospective study. Arthritis Rheum. 2001;44(8):1736–1743. [PubMed]
11. Plant MJ, Williams AL, O'Sullivan MM, Lewis PA, Coles EC, Jessop JD. Relationship between time-integrated C-reactive protein levels and radiologic progression in patients with rheumatoid arthritis. Arthritis Rheum. 2000;43(7):1473–1477. [PubMed]
12. Hoff M, Haugeberg G, Odegard S, Syversen S, Landewe R, van der Heijde D, et al. Cortical hand bone loss after 1 year in early rheumatoid arthritis predicts radiographic hand joint damage at 5-year and 10-year follow-up. Ann Rheum Dis. 2009;68(3):324–329. [PubMed]
13. Haugeberg G, Green MJ, Conaghan PG, Quinn M, Wakefield R, Proudman SM, et al. Hand bone densitometry: a more sensitive standard for the assessment of early bone damage in rheumatoid arthritis. Ann Rheum Dis. 2007;66(11):1513–1517. [PMC free article] [PubMed]
14. Stewart A, Mackenzie LM, Black AJ, Reid DM. Predicting erosive disease in rheumatoid arthritis. A longitudinal study of changes in bone density using digital X-ray radiogrammetry: a pilot study. Rheumatology (Oxford) 2004;43(12):1561–1564. [PubMed]
15. Forslind K, Keller C, Svensson B, Hafstrom I. Reduced bone mineral density in early rheumatoid arthritis is associated with radiological joint damage at baseline and after 2 years in women. J Rheumatol. 2003;30(12):2590–2596. [PubMed]
16. Solomon DH, Finkelstein JS, Shadick N, LeBoff MS, Winalski CS, Stedman M, et al. The relationship between focal erosions and generalized osteoporosis in postmenopausal women with rheumatoid arthritis. Arthritis Rheum. 2009;60(6):1624–1631. [PMC free article] [PubMed]
17. Lodder MC, Haugeberg G, Lems WF, Uhlig T, Orstavik RE, Kostense PJ, et al. Radiographic damage associated with low bone mineral density and vertebral deformities in rheumatoid arthritis: the Oslo-Truro-Amsterdam (OSTRA) collaborative study. Arthritis Rheum. 2003;49(2):209–215. [PubMed]
18. Forsblad D’Elia H, Larsen A, Waltbrand E, Kvist G, Mellstrom D, Saxne T, et al. Radiographic joint destruction in postmenopausal rheumatoid arthritis is strongly associated with generalised osteoporosis. Ann Rheum Dis. 2003;62(7):617–623. [PMC free article] [PubMed]
19. Haugeberg G, Lodder MC, Lems WF, Uhlig T, Orstavik RE, Dijkmans BA, et al. Hand cortical bone mass and its associations with radiographic joint damage and fractures in 50–70 year old female patients with rheumatoid arthritis: cross sectional Oslo-Truro-Amsterdam (OSTRA) collaborative study. Ann Rheum Dis. 2004;63(10):1331–1334. [PMC free article] [PubMed]
20. Bachrach LK, Hastie T, Wang MC, Narasimhan B, Marcus R. Bone mineral acquisition in healthy Asian, Hispanic, black, and Caucasian youth: a longitudinal study. J Clin Endocrinol Metab. 1999;84(12):4702–4712. [PubMed]
21. Wang MC, Aguirre M, Bhudhikanok GS, Kendall CG, Kirsch S, Marcus R, et al. Bone mass and hip axis length in healthy Asian, black, Hispanic, and white American youths. J Bone Miner Res. 1997;12(11):1922–1935. [PubMed]
22. George A, Tracy JK, Meyer WA, Flores RH, Wilson PD, Hochberg MC. Racial differences in bone mineral density in older men. J Bone Miner Res. 2003;18(12):2238–2244. [PubMed]
23. Griffin MR, Ray WA, Fought RL, Melton LJ., 3rd Black-white differences in fracture rates. Am J Epidemiol. 1992;136(11):1378–1385. [PubMed]
24. Braun M, Palacios C, Wigertz K, Jackman LA, Bryant RJ, McCabe LD, et al. Racial differences in skeletal calcium retention in adolescent girls with varied controlled calcium intakes. Am J Clin Nutr. 2007;85(6):1657–1663. [PubMed]
25. Bryant RJ, Wastney ME, Martin BR, Wood O, McCabe GP, Morshidi M, et al. Racial differences in bone turnover and calcium metabolism in adolescent females. J Clin Endocrinol Metab. 2003;88(3):1043–1047. [PubMed]
26. Tracy JK, Meyer WA, Flores RH, Wilson PD, Hochberg MC. Racial differences in rate of decline in bone mass in older men: the Baltimore men's osteoporosis study. J Bone Miner Res. 2005;20(7):1228–1234. [PubMed]
27. Cosman F, Morgan DC, Nieves JW, Shen V, Luckey MM, Dempster DW, et al. Resistance to bone resorbing effects of PTH in black women. J Bone Miner Res. 1997;12(6):958–966. [PubMed]
28. Perry HM, 3rd, Morley JE, Horowitz M, Kaiser FE, Miller DK, Wittert G. Body composition and age in African-American and Caucasian women: relationship to plasma leptin levels. Metabolism. 1997;46(12):1399–1405. [PubMed]
29. Weinstein RS, Bell NH. Diminished rates of bone formation in normal black adults. N Engl J Med. 1988;319(26):1698–1701. [PubMed]
30. Bridges SL, Jr, Hughes LB, Mikuls TR, Howard G, Tiwari HK, Alarcon GS, et al. Early rheumatoid arthritis in African-Americans: the CLEAR Registry. Clin Exp Rheumatol. 2003;21(5 Suppl 31):S138–S145. [PubMed]
31. Arnett FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, Cooper NS, et al. The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis. Arthritis Rheum. 1988;31(3):315–324. [PubMed]
32. Spiegel TM, Spiegel JS, Paulus HE. The joint alignment and motion scale: a simple measure of joint deformity in patients with rheumatoid arthritis. J Rheumatol. 1987;14(5):887–892. [PubMed]
33. Fries JF, Spitz P, Kraines RG, Holman HR. Measurement of patient outcome in arthritis. Arthritis Rheum. 1980;23(2):137–145. [PubMed]
34. Mikuls TR, Holers VM, Parrish L, Kuhn KA, Conn DL, Gilkeson G, et al. Anti-cyclic citrullinated peptide antibody and rheumatoid factor isotypes in African Americans with early rheumatoid arthritis. Arthritis Rheum. 2006;54(9):3057–3059. [PubMed]
35. van der Heijde D. How to read radiographs according to the Sharp/van der Heijde method. J Rheumatol. 1999;26(3):743–745. [PubMed]
36. Genant HK, Grampp S, Gluer CC, Faulkner KG, Jergas M, Engelke K, et al. Universal standardization for dual x-ray absorptiometry: patient and phantom cross-calibration results. J Bone Miner Res. 1994;9(10):1503–1514. [PubMed]
37. Mikuls TR, Saag KG, Curtis J, Bridges SL, Jr, Alarcon GS, Westfall AO, et al. Prevalence of osteoporosis and osteopenia among African Americans with early rheumatoid arthritis: the impact of ethnic-specific normative data. J Natl Med Assoc. 2005;97(8):1155–1160. [PMC free article] [PubMed]
38. Maldonado G, Greenland S. Simulation study of confounder-selection strategies. Am J Epidemiol. 1993;138(11):923–936. [PubMed]
39. Haugeberg G, Uhlig T, Falch JA, Halse JI, Kvien TK. Bone mineral density and frequency of osteoporosis in female patients with rheumatoid arthritis: results from 394 patients in the Oslo County Rheumatoid Arthritis register. Arthritis Rheum. 2000;43(3):522–530. [PubMed]
40. Vis M, Havaardsholm EA, Haugeberg G, Uhlig T, Voskuyl AE, van de Stadt RJ, et al. Evaluation of bone mineral density, bone metabolism, osteoprotegerin and receptor activator of the NFkappaB ligand serum levels during treatment with infliximab in patients with rheumatoid arthritis. Ann Rheum Dis. 2006;65(11):1495–1499. [PMC free article] [PubMed]
41. Walsh NC, Gravallese EM. Bone loss in inflammatory arthritis: mechanisms and treatment strategies. Curr Opin Rheumatol. 2004;16(4):419–427. [PubMed]
42. Sambrook P. Tumour necrosis factor blockade and the risk of osteoporosis: back to the future. Arthritis Res Ther. 2007;9(4):107. [PMC free article] [PubMed]
43. Finckh A, Liang MH, van Herckenrode CM, de Pablo P. Long-term impact of early treatment on radiographic progression in rheumatoid arthritis: A meta-analysis. Arthritis Rheum. 2006;55(6):864–872. [PubMed]
44. Allaire S, Wolfe F, Niu J, Zhang Y, Zhang B, LaValley M. Evaluation of the effect of anti-tumor necrosis factor agent use on rheumatoid arthritis work disability: the jury is still out. Arthritis Rheum. 2008;59(8):1082–1089. [PMC free article] [PubMed]
45. Cohen SB, Dore RK, Lane NE, Ory PA, Peterfy CG, Sharp JT, et al. Denosumab treatment effects on structural damage, bone mineral density, and bone turnover in rheumatoid arthritis: a twelve-month, multicenter, randomized, double-blind, placebo-controlled, phase II clinical trial. Arthritis Rheum. 2008;58(5):1299–1309. [PubMed]
46. McClung MR, Lewiecki EM, Cohen SB, Bolognese MA, Woodson GC, Moffett AH, et al. Denosumab in postmenopausal women with low bone mineral density. N Engl J Med. 2006;354(8):821–831. [PubMed]
47. Jones G, Nguyen T, Sambrook PN, Kelly PJ, Eisman JA. A longitudinal study of the effect of spinal degenerative disease on bone density in the elderly. J Rheumatol. 1995;22(5):932–936. [PubMed]
48. National Osteoporosis Foundation. Clinician’s Guide to Prevention and Treatment of Osteoporosis. Washington, DC: National Osteoporosis Foundation; 2008. [Accessed July 10, 2008]. Available at: http://www.nof.org/professionals/Clinicians_Guide.htm.
49. Ioannidis JP. Why most discovered true associations are inflated. Epidemiology. 2008;19(5):640–648. [PubMed]
50. Solomon DH, Stedman M, Licari A, Weinblatt ME, Maher N, Shadick N. Agreement between patient report and medical record review for medications used for rheumatoid arthritis: the accuracy of self-reported medication information in patient registries. Arthritis Rheum. 2007;57(2):234–239. [PubMed]