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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Expert Opin Ther Targets. Author manuscript; available in PMC 2010 August 16.
Published in final edited form as:
PMCID: PMC2921843
NIHMSID: NIHMS221962

NKT Cell Immune Responses to Viral Infection

Abstract

Background

Natural killer T (NKT) cells are a heterogeneous population of innate T cells that have attracted recent interest because of their potential to regulate immune responses to a variety of pathogens. The most widely studied NKT cell subset is the invariant (i)NKT cells that recognize glycolipids in the context of the CD1d molecule. The multifaceted methods of activation iNKT cells possess and their ability to produce regulatory cytokines has made them a primary target for therapeutic studies.

Objective/Methods

This review gives insight into the roles of iNKT cells during infectious diseases, particularly viral infections. We also highlight the different mechanisms leading to iNKT cell activation in response to pathogens.

Conclusions

The iNKT cell versatility allows them to detect and respond to several viral infections. However, therapeutic approaches to specifically target iNKT cells will require additional research. Notably, examination of the roles of non-invariant NKT cells in response to pathogens warrant further investigations.

Keywords: CD1d, Infectious disease, NKT cells, Viruses

1. INTRODUCTION

An optimal immune response results in the containment, elimination and generation of memory against invading pathogens. The adaptive cells of the immune system provide the specificity necessary to recognize unique antigenic determinants while producing long-lived cells possessing the ability to produce a potent secondary response upon subsequent exposure. In contrast, the cells of the innate immune system respond with a specificity that is limited and fixed. However, their “limitations” provide these cells with the critical ability to respond to an extensive variety of different pathogens. In fact their wide distribution in the host and the diverse array of expressed receptors contribute to the recognition of foreign invaders while bystander cells have the ability to participate through their reaction to cytokine cascades. These critical early reactions influence and shape the down-stream adaptive phase. In this review we discuss a unique subset of T cells, the iNKT cells that express a specific antigen receptor but also resemble innate immune cells through their rapid activation in response to multiple antigens as well as cytokines.

2. iNKT CELLS

Natural killer (NK) T cells were first identified by their expression of an αβ T cell receptor (TCR) and the NK cell activating C-type lectin NK1.1 (CD161, NKR-P1). The invariant NKT cells are the best characterized subset of NKT cells and constitute a unique group of cells that have both innate and adaptive cell surface markers. iNKT cells express a restricted TCR, the Vα24Jα18 chain in humans pairs with Vβ11, and in mice the Vα14Jα18 chain associated with Vβ8.2, 7 and 2 chains. iNKT cells recognize (glyco)lipids presented by the non-classical MHC molecule CD1d [1]. iNKT cells have been described as innate immune effector cells because they are capable of rapidly responding to antigen (Ag), releasing cytokines, proliferating and producing cytolytic mediators, analogous to NK cells [24].

iNKT cells develop in the thymus from common lymphoid progenitors in a developmental pathway similar to that of mainstream T cells. The two lineages eventually diverge and the iNKT cells emerge as CD4+ or double negative (DN) T cell populations with an activated effector memory phenotype CD69+CD62LlowCD44+. iNKT cells exit from the thymus following a CD1d-dependent proliferation event and gain the full expression of NK1.1 in the periphery (for reviews see [25]). iNKT cells make up a relatively small population (~1%) of T cells in mice, primarily located in the liver. Interestingly, examination of a panel of inbred mouse strains recently revealed that the number of liver iNKT cells is quite variable, spanning over a 100-fold range [6].

Although variable percentages of CD8+ NKT cells exist in humans, they have not been detected in mice [711]. In response to pathogens, iNKT cells share common effector functions with conventional T cells and NK cells, including Fas-FasL mediated cytotoxicity, perforin release and cytokine production [12, 13]. The ability to produce both Th1 and Th2 cytokines, IFN-γ and IL-4, along with several other cytokines such as IL-10, IL-2, IL-13, TNF-α and IL-17, allow iNKT cells to act immediately and regulate the down-stream immune response in reaction to pathogens. iNKT cells have been implicated in a variety of immune conditions including autoimmunity, anti-tumor activity and defense against pathogens. This review concentrates on the role of iNKT cells during infectious diseases, highlighting the diverse mechanisms of iNKT cell activation, and ending with a special focus on iNKT cell participation during viral infection.

3. iNKT CELL ANTIGENS

Conventional T cells are dependent on MHC proteins for Ag recognition. In contrast, iNKT cells interact with the non-polymorphic MHC class I like molecule, CD1d. Instead of recognizing proteins presented in the groove of MHC molecules, iNKT cells distinguish particular lipid Ags loaded in the hydrophobic domain of CD1d. CD1d is expressed on all lymphocytes, predominantly on APCs such as dendritic cells (DCs), macrophages (Mϕ) and B cells as well as on liver cell populations including Kupffer cells, hepatic sinusoidal endothelial cells and hepatocytes.

CD1d is a highly conserved member of the immunoglobulin superfamily, present in all mammals and the only CD1 member found in rats and mice. Disruption of CD1d trafficking or lipid loading dramatically impacts the development of iNKT cells. Until recently, the only known ligand shown to activate iNKT cells in the context of CD1d was α-galactosylceramide (α-GalCer). Now, several self and foreign lipids able to stimulate iNKT cells have been identified.

3.1 α-Galactosylceramide

α-GalCer was discovered by the Kirin Brewery Corporation pharmaceutical research division in 1993 as an exogenous glycosphingolipid capable of activating iNKT cells [14]. It was originally isolated from extracts of Agelas mauritinius, a marine sponge, and was found to be effective at preventing the growth of transplanted tumors in mice. A synthetic analogue, KRN700 was developed for use in research and clinical trials [15]. Upon exposure to this lipid Ag, iNKT cells react immediately by dramatically producing both Th1 (IFN-γ) and Th2 (IL-4) cytokines, internalizing some of their cell surface molecules, notably NK1.1 and the TCR whereas some iNKT cells undergo activation induced cell death (AICD). The explosive response to α-GalCer is limited to the iNKT cell population, but the downstream result is the indirect activation of NK cells, DC, Mϕ and B cells [1622].

3.2 Isoglobotrihexosylceramide (iGb3)

iGb3 is a lysosomal glycosphigolipid that was first identified following the observation that mice deficient in the beta subunit of β-hexosaminidase, the enzyme that converts iGb4 to iGb3, displayed a specific loss of iNKT cells. A chemically synthesized iGb3 molecule was shown to specifically activate both human and mouse iNKT cells in vitro and was identified in the human thymus [23, 24]. These data led the author to suggest that iGb3 was the main endogenous ligand responsible for NKT cell development. However, this claim has recently been challenged [2527].

3.3 Microbial glycolipids

Microbial glycosylceramides and diacylglycerol Ag, which strongly activate iNKT cells, have also been characterized [2830]. These lipids are structurally similar to α-GalCer and are found in the cell wall of Gram-negative, LPS deficient bacteria such as Sphingomonas capsulata, Ehrlichia muris, and Borrelia burgdoferi (reviewed in [4, 31] and see below).

4. iNKT CELLS AND NON-VIRAL PATHOGENS

In order to explore the function of iNKT cells, many studies have implemented the use of pathogens including bacteria, parasites and viruses. The use of two mouse strains with a deficiency in iNKT cells is often employed to examine the role of this subset, the Jα18−/− mice, specifically lacking the gene segment required to form the invariant α-chain, (iNKT cell deficient) and CD1d−/− mice, which as mentioned previously, fail to select iNKT and other CD1d restricted cells in the thymus. Additionally, WT mice have been treated with anti-CD1d antibodies (Abs) in order to block CD1d functions thereby preventing Ag presentation to the invariant TCR or α-GalCer administered to activate the iNKT compartment. In this review we will describe the diverse microbial pathogens that involve iNKT cell responses with particular attention given to viral infections.

4.1 BACTERIA

Indicative of its ability to recognize lipid Ags in the context of CD1d, the Vα14 TCR may have evolved to recognize microbial lipids expressed on bacterial cell walls. There is current evidence that iNKT cells play a role in controlling gram-negative α-proteobacteria infections, such as Sphingomonas species, capable of severe infections in immunocompromised hosts, and the tickborn diseases Ehrlichia muris and Rickettsia [32, 33]. The absence of iNKT cells results in delayed clearance of these bacteria and increased mortality. Conversely, a high dose challenge with Sphingomonas in an immunocompetent host results in lethal septic shock, while iNKT cell deficient mice are protected from this consequence [32, 33]. iNKT cells recognize certain glycosphingolipids from Sphingomonas through CD1d presentation to the invariant TCR of both mouse and human [30, 32]. The Borrelia burgdorferi glycolipid α-linked mono-galactosyl diacylglycerol, which has broad structural similarity to α-GalCer, can also activate iNKT cells directly through the TCR to stimulate proliferation and cytokine production for both mouse and human iNKT cells [31]. Borrelia burgdorferi is the causative agent of Lyme disease, manifesting variable symptoms including arthritis. Interestingly, CD1d−/− mice infected with B. burgdorferi have increased incidence of joint inflammation, spirochete DNA in the urinary bladder and secrete the IgG2a isotope commonly associate with susceptibility [34]. Resistance is dependent on appropriate B cell contribution and passive immunization can protect susceptible mouse strains.

Research involving Salmonella has presented an alternative method for iNKT cell activation. Salmonella infection causes a spectrum of diseases by infecting the host through the oral route whereby bacteria can disseminate to other organs. Salmonella activate DCs to produce IL-12 through Toll-like receptor 4 (TLR4) stimulation by LPS. Activated DCs present endogenous Ag via CD1d to iNKT cells during infection [33, 35]. The co-requirement for IL-12 and CD1d revealed a unique and indirect mechanism for activating iNKT cells, where self-ligand(s) such as iGb3 are presented rather than a microbial Ag. However, LPS-induced iNKT cell-derived IFN-γ in vitro does not require CD1d-mediated Ag presentation, instead exposure to IL-12 and IL-18 is sufficient to activate these cells [36].

iNKT cells have also been implicated in Pseudomonas aeruginosa infection in vivo but these findings have been recently challenged [37]. Similarly, although iNKT cells produce IFN-γ during Listeria monocytogenes infection, the role for iNKT cells and/or CD1d is not clear [3840].

4.2 PARASITES

Studies on visceral Leishmania donovani suggest that lipophosphoglycan, or glycoinositol phospholipids on the surface of L. donovani bind to CD1d molecules and can be recognized by iNKT cells. Furthermore, infected CD1d−/− mice develop a defective granuloma response and have a higher parasite burden in the liver and spleen during the innate response compared to WT controls [41]. In this study, iNKT cells appear to make IFN-γ immediately after parasite inoculation followed by the reduction of detectable iNKT cells. Additionally, iNKT cells appear to regulate sustained hepatic CXCL10 mRNA expression during the early stages of infection [42]. However, a recent study shows that iNKT cells on the C57BL/6 background only play a minor role in the overall protection against L. donovani, where no significant differences between iNKT deficient mice vs WT controls were found in the chronic stages of infection [43].

Cutaneous L. major infection also provides evidence for a protective role of iNKT cells where parasite numbers increase in NKT cell deficient mice during the early stages of infection [44, 45]. The most dramatic differences were seen after intravenous introduction of parasites vs subcutaneous infection where there was a 10–50 fold parasite increase seen in the spleens of NKT cell deficient mice as well as decreased NK cell IFN-γ production. It is of interest that many of the discrepancies between publication results may be due to the strain of mouse used, the route of infection and the strain of the parasite.

Using a mouse model of Trypanosoma cruzi infection, WT and CD1d−/− mice both develop mild phenotypic symptoms, but the majority of the mice survive [46, 47]. However, the same inoculum given to Jα18−/− mice, results in a dramatic increase in mortality and morbidity [46]. Additionally, the production of inflammatory cytokines is significantly enhanced in Jα18−/− animals. Furthermore, GPI mucins and GIPLs from the surface of T. cruzi bind to CD1d molecules and inhibit α-GalCer activation of NKT cell hybridomas, but these ligands alone do not appear to activate iNKT cells. These results suggest a striking contrast in function between iNKT cells and other subsets of NKT cells that are present in the Jα18−/− mice, but not in CD1d−/− mice [47].

The role of iNKT cells during helminth infection has recently been appreciated [48]. New findings suggest that, although dispensable for host resistance, iNKT cells play a part in the development of the acquired immune response and in the control of pathology during murine schistosomiasis. Here too, major differences were observed between Jα18−/− mice and CD1d−/− mice [49]. Of note, schistosome eggs appear to be the only parasitic stage capable of activating iNKT cells in a TCR dependent manner, although the mechanisms still remain unresolved [50].

5. iNKT CELLS AND VIRUS

While the speculation and search for pathogenic ligands for the Vα14i TCR continue in respect of different bacterial, parasitic and fungal pathogens, viral genomes do not generate lipid molecules. Therefore the mechanism of iNKT cell activation during viral infection must use either host lipids in the context of CD1d or an entirely TCR-independent mechanism.

5.1 HIV

Human immunodeficiency virus (HIV) targets iNKT cells, which are subsequently depleted from the host [7, 51]. Viral entry in iNKT cells requires the expression of the chemokine receptors CCR5 or CXCR4 in combination with CD4 [52], although the loss of CD4 iNKT cells also occurs. The R5-tropic HIV-1 strain replicates in CD4+ iNKT cells more vigorously than in mainstream T cells, which might account for their rapid selective loss. In fact, high viremia is correlated with lower numbers of circulating NKT cells. Alternatively, it is possible that iNKT cell reduction occurs from the direct or indirect activation by APCs leading to AICD. The loss of iNKT cells during HIV infection has a number of potentially harmful consequences. Decreased NKT cell surveillance could be linked to the increased incidence of certain AIDS related tumors such as Kaposi’s Sarcoma.

Recently it was shown that CD1d expression is decreased, particularly on CD14+ monocytes, in HIV-infected individuals compared to highly active antiretroviral therapy (HAART) patients or healthy donors. Akin to loss of iNKT cells, CD1d expression is inversely correlated to viral load. The reduction of CD1d is caused by the HIV-1 protein Nef, which physically associates with the cytoplasmic tail of CD1d interfering with its surface expression [53, 54]. As predicted, down-regulation of CD1d was shown for both the human and mouse homologues indicating that Nef targets a highly conserved molecular domain [53, 54]. Diminished CD1d expression may negatively influence the ability of iNKT cells to recognize infected cells that have up-regulated endogenous ligands.

While HAART is known to reduce viral load in HIV infected patients, it also results in the recovery of predominantly CD4 NKT cells along with conventional CD4+ T cells [55]. The rapid restoration of circulating iNKT cells is speculated to be the result of their redistribution from tissue sequestration. The recovered iNKT cells retain their functional capacity and cytokine profiles. Concurrent administration of α-GalCer with low-dose DNA vaccine enhances both cellular and humoral responses to HIV infection [56]. While HIV therapy has improved the outcome for many infected-individuals, results can be variable depending on differences in disease stages, genetic make up and factors that are outside the bounds of experimental control.

5.2 EMCV

Encephalomyocarditic virus (EMCV) is a picornavirus that causes acute diabetes, paralysis and myocarditis. Resistance to EMCV is dependent on the early production of adequate amounts of IL-12 induced production of IFN-γ from NK cells. Studies comparing WT mice with CD1d−/− mice revealed additional details of the innate-adaptive pathway involved in viral clearance. Sensitive BALB/c mice, are protected from EMCV through treatment with α-GalCer [57] whereas CD1d−/− mice on 129 and B6 backgrounds show increased susceptibility to EMCV infection [58]. CD1d−/− mice produce less IFN-α and IL-12, causing decreased IFN-γ in response to ECMV than their WT counterparts. This cytokine axis results in the inverse correlation between viremia and CD8+ T cell activation [59]. Administration of IL-12 in the CD1d−/− mice improves immune resistance, bypassing the contributions of iNKT cells and acting directly to increase IFN-γ production by NK cells. Furthermore, in vitro addition of anti-CD1d mAb increased EMCV replication in WT splenocyte cultures. However, Jα18−/− mice do not show enhanced susceptibility suggesting that iNKT cells are not required for EMCV resistance, but another subset of CD1d-dependent cells has a protective role.

5.3 LCMV

Lymphocytic Choriomeningitis Virus (LCMV) is a natural mouse pathogen that has been useful in the study of T cell responses to viral infection. The Armstrong strain of LCMV has been shown to induce the loss of hepatic, splenic and peritoneal NKT cells [60]. This loss is recovered within the liver by two weeks post-infection (p.i.), but persists in the spleen for over three months. This depletion is independent of Fas-FasL interactions as well as IFN-γ and IL-12, cytokines typically produced during LCMV infection. While it was shown that iNKT cells are actively infected by LCMV, the means of compartmental loss occurs through the immediate production of IFN-α/β, which subsequently causes iNKT cell apoptosis.

Interestingly, the employment of CD1d−/− mice demonstrated the regulatory role iNKT or/and non-invariant CD1d restricted cells have during LCMV. CD1d−/− mice show elevated levels of IL-2, IL-4 and IFN-γ in response to LCMV and the cytokine production is maintained even after viral clearance has occurred [61]. The cytokine rich environment enhances the proliferation of T cell subsets, further augmenting cytokine potential and the ability to clear virus more quickly. Both DCs and Mϕ exhibit down-regulation of CD1d at day 10 p.i. following a substantial increase seen at day 6, also independent of IFN-γ or IL-12 [62, 63]. However, both WT and CD1d−/− mice are capable of eliminating virus.

5.4 INFLUENZA

Influenza A virus (IAV) infection rapidly spreads around the world in seasonal epidemics and imposes a considerable economic burden. Furthermore, pandemics with highly virulent strains or transference of humans with H5N1 (bird flu) viruses represent current health threats. Of particular interest is the recent demonstration that α-GalCer can serve as a potent mucosal adjuvant to trigger protection against IAV infection [64]. Intranasal (i.n.) co-administration of α-GalCer with either hemagglutinin [65] or inactivated IAV [64], induces long-lasting protective mucosal and systemic immune responses against lethal infection with IAV in the mouse system. More recently, Kamijuku et al. provided evidence that i.n. vaccination with α-GalCer plus hemagglutinin induces an effective cross-protection against different strains of influenza virus, including H5N1 [66]. This vaccine protocol resulted in the CXCL16/CXCR6 dependent increase of nasal mucosa iNKT cells, IL-4 dependent increase of IgA secretion and improved survival from influenza induced lethal pneumonia. Finally, exogenous activation of iNKT cells by means of α-GalCer administration during IAV infection was shown to enhance the early innate immune response in the lungs and contribute to antiviral immunity [67]. As a whole, these studies offer new methods for combating the high mortality rate of IAV infection by targeting iNKT cells and encouraging the development of α-GalCer, or other iNKT-activating glycolipid adjuvants. Importantly, De Santo and colleagues recently demonstrated that iNKT cells play a critical role in controlling IAV PR8 virus infection and that this effect is CD1d dependent [68].

5.5 RSV

Respiratory syncytial virus (RSV), an RNA virus in the family Paramyxoviridae, causes respiratory disease in humans. It is the most common cause of lower respiratory tract infection in infants and children worldwide. The immune response to primary RSV infection in humans and mice is generally characterized by a mixed Th1/Th2 cytokine response [6971]. Johnson et al. examined the role of CD1d expression and Ag presentation in RSV pathogenesis using CD1d-deficient mice and α-GalCer. They found that iNKT cells contribute to the efficient induction of CD8+ T cell responses and amplification of antiviral immune responses to respiratory syncytial virus. They proposed that the absence of CD1d restricted T cell (invariant or/and non-invariant) activation leads to a reduction in early IFN-γ production, resulting in diminished RSV specific CD8+ T cell expansion and delayed viral clearance. [72].

5.6 TMEV

Theiler's murine encephalomyelitis virus (TMEV) causes demyelination with inflammation of the central nervous system in mice and is used as an animal model for multiple sclerosis (MS). On one hand NKT cells have been shown to play a protective role against demyelination, while other investigators demonstrated no function or a detrimental role for NKT cells [7377]. In a recent study, CD1d restricted NKT cells were shown to play a protective role in TMEV induced neurological disease by alteration of the cytokine profile and virus-specific immune responses. The authors demonstrated that the CD1d−/− mice developed demyelinating disease with more neurological deficits and higher IL-4 production compared to the WT mice [78].

5.7 HSV

Investigation of herpesvirus family members has resulted in divergent results depending on the strain used or the amount of virus inoculated. An early study using the virulent herpes simplex virus (HSV)-1 SC16 strain demonstrated that CD1d−/− and Jα18−/− mice were impaired in their ability to clear the virus, showing increased skin lesions, greater morbidity, viral persistence and increased spread of HSV to the nervous system [79]. Recently, Grubor-Bauk et al. showed the importance of iNKT cells in resistance to HSV and their contribution to the level of latency in mice [80]. In contrast, the HSV-1 less virulent Kos strain exemplified no dissimilarity between NKT cell deficient mice vs WT controls [81].

Two recent reports indirectly support a potential role for iNKT cells in the clearance of the HSV-1 infection. Yuan et al. demonstrated that infection with HSV-1 reduces CD1d cell surface expression on APCs. In this case, HSV-1 prevents the reappearance of endocytosed CD1d on the cell surface by redistributing endocytosed CD1d to the lysosome limiting membrane [82]. Raftery et al showed that HSV-1 strain F also affects CD1d expression but it is dependent on the dose of administered viruses. Low MOI increases CD1d expression on DCs and causes iNKT proliferation in vitro, while high MOI decreases CD1d expression [83]. Recently, Kaposi sarcoma-associated herpesvirus (KSHV) was reported to decrease human CD1d as well [84]. The viral protein modulator of immune recognition is an ubiquitin ligase that associates with the cytoplasmic tail of CD1d, causing cell-surface down-regulation. Interestingly, as mentioned with HIV, the protein Nef influences CD1d expression, which may in part, be influential in aiding this opportunistic virus. Altogether, these results suggest that inhibition of CD1d surface expression may be an important HSV-1 immune evasion strategy.

5.8 MCMV & HCMV

The cytomegaloviruses (CMVs) are ubiquitous species-specific β-herpesviruses. CMV is a double stranded DNA virus containing more than 240kb, and encoding over 200 potential proteins, making it the largest member of the herpesvirus family [85]. Typically, infection is asymptomatic in immunocompetent hosts, but can result in morbidity and mortality in high-risk individuals. The replication cycle of CMV is divided into the immediate, early, and late phases according to the time of gene transcription. Murine CMV (MCMV) is widely used as an experimental model for human CMV and has been successfully used to elucidate the mechanisms involved in virulence, immune evasion and immune detection. Host resistance is dependent on both innate NK cell containment, followed by the specific adaptive CD8+ T cell elimination of the virus. The critical involvement of NK cells has been demonstrated for both HCMV and MCMV. Individuals lacking a functional NK cell compartment are subject to severe infection and increased mortality to HCMV. Studies in different strains of mice have revealed that not only is the presence of functional NK cells necessary to control MCMV, but the presence of specific NK cell surface receptors is key for orchestrating the successful elimination of the virus. NK cell effector functions are initiated by the production of type I IFN and IL-12, which stimulate NK cell production of IFN-γ, proliferation and cytotoxic activity. The initial activation signal appears to be non-specific, acting on the bulk of the NK cell compartment, however, by day 2–3 of the acute phase, a selective proliferation occurs within the Ly49H+ NK cell subset [86, 87]. Mice lacking the activating receptor Ly49H, BALB/c and 129 strains, are susceptible to MCMV, while C57BL/6 are resistant due to the presence of the gene Cmv1r, encoding this protein [8890]. Ly49H interacts directly with the virus encoded gene product m157 that becomes expressed on the surface of MCMV infected cells [91, 92]. The activation signaling cascade instigated by this association involves the adaptor protein DAP12 and results in the production of traditional NK cell effector functions in the Ly49H+ subset [93]. In other strains of mice, (K haplotype), protection in vivo requires the activating receptor Ly49P recognition of Dk carrying virus peptide fragments on infected cells [94]. Additional analysis uncovered that the selective NK cell response is also dependent on IL-18, IL-12 and interactions with CD8α+ DCs [95].

Initially, an attempt to determine a role for activated iNKT cells as a potential target for MCMV immunotherapy was considered. α-GalCer was administered at different times of inoculation using the virulent K181-Perth strain of MCMV [96]. The investigators determined that α-GalCer therapy resulted in reduced viral titers in the spleen and liver of both susceptible BALB/c and resistant B6 mice. The therapeutic effects of α-GalCer activated iNKT cells was dependent on their influence over NK cells, as mice depleted of NK cells by α-asialo GM1 treatment, did not limit viral replication.

Recently, iNKT cell participation during MCMV infection was examined [97, 98]. Two studies found that iNKT cells dynamically participate in the initial immune response to MCMV infection [97]. Indeed, shortly after inoculation, iNKT cells display signs of activation, up-regulation of the high affinity receptor CD25, decrease in cell numbers in the spleen and liver and robust production of IFN-γ [97, 98]. Interestingly, iNKT cells react to MCMV as innate immune cells rather than having an adaptive role as an interaction between the TCR and CD1d is dispensable for the activation phenotype and cytokine release [97, 98]. Both blocking the invariant TCR recognition with anti-CD1d Abs and the use of adoptively transferred iNKT cells into CD1d−/− and Jα18−/− hosts results in both iNKT and NK cell production of IFN-γ. However, while the TCR was not vital to the outcome, IFN-γ production is at least partially dependent on IL-12 and IFNα/β secretion [97]. Tyznik et al. subsequently demonstrated that the mechanism of the iNKT response was through indirect activation [98]. MCMV elicits the activation of DCs in a TLR9 dependent fashion, stimulating the production of IL-12, which subsequently activates the iNKT cells to produce IFN-γ.

However, Jα18−/− mice do not succumb to high dose MCMV infection more frequently than their WT counterparts [96, 97], and hence iNKT cells appear to be dispensable for control of MCMV infection at least in the B6 background. Interestingly, approximately 50% of CD1d−/− mice show increased susceptibility to MCMV than control littermates, revealing a potential role for other CD1d dependent cells having a protective role during the later stage of infection [97].

CONCLUDING REMARKS

iNKT cells have been implicated, for better or worse, in a number of different microbial infections. This review attempts to represent just a few in order to depict the range of function and multiple mechanisms of activation that iNKT cells possess. We have highlighted the diverse ways in which iNKT cells are activated by microbes. First, CD1d presentation of endogenous ligands or exogenous pathogen derived ligands by APCs. Additionally, iNKT cell activation can be driven by different cytokines directly or indirectly, IL-12, IL-18, type I and II IFNs, or any combination of stimuli. Microorganisms have evolved different mechanisms in order to evade immune detection and interfere with both innate and adaptive effector abilities. Depending on the pathogen, iNKT cells have the ability to respond rapidly and regulate different compartments of the immune system both innate and adaptive. These studies demonstrate that therapies aimed at modulating the iNKT response may have beneficial or adverse outcomes, but are valuable targets for therapeutics.

EXPERT OPINION

iNKT cells play a multifaceted role in a variety of immune responses in part by bridging innate and adaptive immunity. iNKT cells have been extensively studied in the context of a wide range of infectious agents. Although the mechanisms by which iNKT cells engage in viral immunity need to be fully elucidated, the current studies emphasize the importance of accurately analyzing the functionality of iNKT cells in models of viral infection before they can be exploited in therapeutic settings. It is clear that iNKT cells have the competency to promote a spectrum of immunoregulatory responses and hold great promise for development of vaccine adjuvant and immunotherapies. For instance, Huang et al. illustrated that α-GalCer enhances the immunogenecity of DNA vaccines, which may aid in designing more effective adjuvant and vaccines against HIV-1 [56]. Similarly, new methods for fighting the flu using iNKT cell targets could provide effective cross-protective mucosal immunity to multiple influenza strains [6668].

In spite of this, iNKT cells are often considered a “double edged sword”, particularly in the pathogenesis of certain infections causing liver disease. For instance, iNKT cells exert both anti- and proinflammatory responses to hepatitis. Despite the presence of abundant hepatic iNKT cells, treatment with α-GalCer is necessary to promote an effective antiviral response to hepatitis B virus (HBV) in transgenic mice bearing a HBV genome [99]. Additionally, Ito et al showed that activation of iNKT cells promotes the breakage of CTL tolerance in the setting of HBV induced hepatitis [100]. In contrast, iNKT cells are negatively implicated in the regeneration process of the liver in a HPV partial hepatectomy model. This was speculated to be, in part, a negative effect of IFN-γ on hepatocytes [101].

Importantly, administration of α-GalCer can cause iNKT cells to become unresponsive, raising the issue of anergy induction in designing treatment regimens that use specific activators of iNKT cells [102, 103]. Similarly, it has been demonstrated that iNKT cells activated in response to multiple bacterial microorganisms acquire a hyporesponsive phenotype, which can significantly impact subsequent iNKT cell–mediated immune responses and the efficacy of iNKT cell–based immunotherapy [104]. Therefore, therapeutic approaches that specifically stimulate iNKT cells might need to be combined with systems that target inhibitory receptors such as programmed cell death 1 or the neutralization of IL-10 [105].

Finally, non-invariant CD1d restricted T cells contribute significantly to the innate immune response to several pathogens, illustrated by the different phenotypes observed when the immune response from Jα18−/− and CD1d−/− mice is compared. For instance, CD1d deficient mice are more sensitive to MCMV and ECMV infections than Jα18−/− mice [57, 97]. Therefore the characterization of non-classical NKT cells as well as identification of their specific ligands warrant further investigations.

REFERENCES

1. Brigl M, Brenner MB. CD1: Antigen Presentation and T Cell Function. Annu Rev Immunol. 2004;22:817–890. [PubMed]
2. Kronenberg M. Toward an Understanding of NKT Cell Biology: Progress and Paradoxes. Annu Rev Immunol. 2004 Sep 27;23:877–900. [PubMed]
3. Godfrey DI, MacDonald HR, Kronenberg M, Smyth MJ, Van Kaer L. NKT cells: what's in a name? Nat Rev Immunol. 2004 Mar;4(3):231–237. [PubMed]
4. Bendelac A, Savage PB, Teyton L. The Biology of NKT Cells. Annu Rev Immunol. 2006 Dec 6;25:297–336. [PubMed]
5. Matsuda JL, Gapin L. Developmental program of mouse Va14i NKT cells. Curr Opin Immunol. 2005 Apr;17(2):122–130. [PubMed]
6. Rymarchyk SL, Lowenstein H, Mayette J, Foster SR, Damby DE, Howe IW, et al. Widespread natural variation in murine natural killer T-cell number and function. Immunology. 2008 Apr 26; [PubMed]
7. Motsinger A, Haas DW, Stanic AK, Van Kaer L, Joyce S, Unutmaz D. CD1d-restricted human natural killer T cells are highly susceptible to human immunodeficiency virus 1 infection. J Exp Med. 2002 Apr 1;195(7):869–879. [PMC free article] [PubMed]
8. Lee PT, Benlagha K, Teyton L, Bendelac A. Distinct functional lineages of human V(alpha)24 natural killer T cells. J Exp Med. 2002 Mar 4;195(5):637–641. [PMC free article] [PubMed]
9. Couedel C, Peyrat MA, Brossay L, Koezuka Y, Porcelli SA, Davodeau F, et al. Diverse CD1d-restricted reactivity patterns of human T cells bearing "invariant" AV24BV11 TCR. Eur J Immunol. 1998 Dec;28(12):4391–4397. [PubMed]
10. Gumperz JE, Miyake S, Yamamura T, Brenner MB. Functionally distinct subsets of CD1d-restricted natural killer T cells revealed by CD1d tetramer staining. J Exp Med. 2002 Mar 4;195(5):625–636. [PMC free article] [PubMed]
11. Takahashi T, Nieda M, Koezuka Y, Nicol A, Porcelli SA, Ishikawa Y, et al. Analysis of human V alpha 24+ CD4+ NKT cells activated by alpha-glycosylceramide-pulsed monocyte-derived dendritic cells. J Immunol. 2000 May 1;164(9):4458–4464. [PubMed]
12. Metelitsa LS, Naidenko OV, Kant A, Wu HW, Loza MJ, Perussia B, et al. Human NKT cells mediate antitumor cytotoxicity directly by recognizing target cell CD1d with bound ligand or indirectly by producing IL-2 to activate NK cells. J Immunol. 2001 Sep 15;167(6):3114–3122. [PubMed]
13. Kawano T, Nakayama T, Kamada N, Kaneko Y, Harada M, Ogura N, et al. Antitumor cytotoxicity mediated by ligand-activated human V alpha24 NKT cells. Cancer Res. 1999 Oct 15;59(20):5102–5105. [PubMed]
14. Kawano T, Cui J, Koezuka Y, Toura I, Kaneko Y, Motoki K, et al. CD1d-restricted and TCR-mediated activation of Vα14 NKT cells by glycosylceramides. Science. 1997;278(5343):1626–1629. [PubMed]
15. Kobayashi E, Motoki K, Uchida T, Fukushima H, Koezuka Y. KRN7000, a novel immunomodulator, and its antitumor activities. Oncol Res. 1995;7(10–11):529–534. [PubMed]
16. Carnaud C, Lee D, Donnars O, Park SH, Beavis A, Koezuka Y, et al. Cutting edge: Cross-talk between cells of the innate immune system: NKT cells rapidly activate NK cells. J Immunol. 1999 Nov 1;163(9):4647–4650. [PubMed]
17. Eberl G, MacDonald HR. Selective induction of NK cell proliferation and cytotoxicity by activated NKT cells. Eur J Immunol. 2000;30(4):985–992. [PubMed]
18. Burdin N, Brossay L, Kronenberg M. Immunization with α-galactosylceramide polarizes CD1-reactive NK T cells towards Th2 cytokine synthesis. Eur J Immunol. 1999;29(6):2014–2025. [PubMed]
19. Hermans IF, Silk JD, Gileadi U, Salio M, Mathew B, Ritter G, et al. NKT cells enhance CD4+ and CD8+ T cell responses to soluble antigen in vivo through direct interaction with dendritic cells. J Immunol. 2003 Nov 15;171(10):5140–5147. [PubMed]
20. Fujii S, Shimizu K, Smith C, Bonifaz L, Steinman RM. Activation of natural killer T cells by α-galactosylceramide rapidly induces the full maturation of dendritic cells in vivo and thereby acts as an adjuvant for combined CD4 and CD8 T cell immunity to a coadministered protein. J Exp Med. 2003 Jul 21;198(2):267–279. [PMC free article] [PubMed]
21. Galli G, Nuti S, Tavarini S, Galli-Stampino L, De Lalla C, Casorati G. CD1d-restricted Help To B Cells By Human Invariant Natural Killer T Lymphocytes. J Exp Med. 2003 April 21;197(8):1051–1057. 2003. [PMC free article] [PubMed]
22. Wesley JD, Robbins SH, Sidobre S, Kronenberg M, Terrizzi S, Brossay L. Cutting edge: IFN-gamma signaling to macrophages is required for optimal Valpha14i NK T/NK cell cross-talk. J Immunol. 2005 Apr 1;174(7):3864–3868. [PubMed]
23. Zhou D, Mattner J, Cantu C, 3rd, Schrantz N, Yin N, Gao Y, et al. Lysosomal glycosphingolipid recognition by NKT cells. Science. 2004 Dec 3;306(5702):1786–1789. [PubMed]
24. Li Y, Teneberg S, Thapa P, Bendelac A, Levery SB, Zhou D. Sensitive detection of isoglobo and globo series tetraglycosylceramides in human thymus by ion trap mass spectrometry. Glycobiology. 2008 Feb;18(2):158–165. [PubMed]
25. Speak AO, Salio M, Neville DC, Fontaine J, Priestman DA, Platt N, et al. From the Cover: Implications for invariant natural killer T cell ligands due to the restricted presence of isoglobotrihexosylceramide in mammals. Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):5971–5976. [PubMed]
26. Porubsky S, Speak AO, Luckow B, Cerundolo V, Platt FM, Grone HJ. From the Cover: Normal development and function of invariant natural killer T cells in mice with isoglobotrihexosylceramide (iGb3) deficiency. Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):5977–5982. [PubMed]
27. Christiansen D, Milland J, Mouhtouris E, Vaughan H, Pellicci DG, McConville MJ, et al. Humans lack iGb3 due to the absence of functional iGb3-synthase: implications for NKT cell development and transplantation. PLoS Biol. 2008 Jul 15;6(7):e172. [PMC free article] [PubMed]
28. Wu D, Xing GW, Poles MA, Horowitz A, Kinjo Y, Sullivan B, et al. Bacterial glycolipids and analogs as antigens for CD1d-restricted NKT cells. Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1351–1356. [PubMed]
29. Zajonc DM, Cantu C, 3rd, Mattner J, Zhou D, Savage PB, Bendelac A, et al. Structure and function of a potent agonist for the semi-invariant natural killer T cell receptor. Nat Immunol. 2005 Aug;6(8):810–818. [PMC free article] [PubMed]
30. Sriram V, Du W, Gervay-Hague J, Brutkiewicz RR. Cell wall glycosphingolipids of Sphingomonas paucimobilis are CD1d-specific ligands for NKT cells. Eur J Immunol. 2005 Jun;35(6):1692–1701. [PubMed]
31. Kinjo Y, Tupin E, Wu D, Fujio M, Garcia-Navarro R, Benhnia MR, et al. Natural killer T cells recognize diacylglycerol antigens from pathogenic bacteria. Nat Immunol. 2006 Sep;7(9):978–986. [PubMed]
32. Kinjo Y, Wu D, Kim G, Xing GW, Poles MA, Ho DD, et al. Recognition of bacterial glycosphingolipids by natural killer T cells. Nature. 2005 Mar 24;434(7032):520–525. [PubMed]
33. Mattner J, Debord KL, Ismail N, Goff RD, Cantu C, 3rd, Zhou D, et al. Exogenous and endogenous glycolipid antigens activate NKT cells during microbial infections. Nature. 2005 Mar 24;434(7032):525–529. [PubMed]
34. Kumar H, Belperron A, Barthold SW, Bockenstedt LK. Cutting edge: CD1d deficiency impairs murine host defense against the spirochete, Borrelia burgdorferi. J Immunol. 2000 Nov 1;165(9):4797–4801. [PubMed]
35. Brigl M, Bry L, Kent SC, Gumperz JE, Brenner MB. Mechanism of CD1d-restricted natural killer T cell activation during microbial infection. Nat Immunol. 2003 Dec;4(12):1230–1237. [PubMed]
36. Nagarajan NA, Kronenberg M. Invariant NKT cells amplify the innate immune response to lipopolysaccharide. J Immunol. 2007 Mar 1;178(5):2706–2713. [PubMed]
37. Nieuwenhuis EE, Matsumoto T, Exley M, Schleipman RA, Glickman J, Bailey DT, et al. CD1d-dependent macrophage-mediated clearance of Pseudomonas aeruginosa from lung. Nat Med. 2002 Jun;8(6):588–593. [PubMed]
38. Szalay G, Ladel CH, Blum C, Brossay L, Kronenberg M, Kaufmann SH. Cutting edge: anti-CD1 monoclonal antibody treatment reverses the production patterns of TGF-beta 2 and Th1 cytokines and ameliorates listeriosis in mice. J Immunol. 1999 Jun 15;162(12):6955–6958. [PubMed]
39. Arrunategui-Correa V, Lenz L, Kim HS. CD1d-independent regulation of NKT cell migration and cytokine production upon Listeria monocytogenes infection. Cell Immunol. 2004 Nov–Dec;232(1–2):38–48. [PubMed]
40. Ranson T, Bregenholt S, Lehuen A, Gaillot O, Leite-de-Moraes MC, Herbelin A, et al. Invariant V alpha 14+ NKT cells participate in the early response to enteric Listeria monocytogenes infection. J Immunol. 2005 Jul 15;175(2):1137–1144. [PubMed]
41. Amprey JL, Im JS, Turco SJ, Murray HW, Illarionov PA, Besra GS, et al. A subset of liver NK T cells is activated during Leishmania donovani infection by CD1d-bound lipophosphoglycan. J Exp Med. 2004 Oct 4;200(7):895–904. [PMC free article] [PubMed]
42. Svensson M, Zubairi S, Maroof A, Kazi F, Taniguchi M, Kaye PM. Invariant NKT cells are essential for the regulation of hepatic CXCL10 gene expression during Leishmania donovani infection. Infect Immun. 2005 Nov;73(11):7541–7547. [PMC free article] [PubMed]
43. Stanley AC, Zhou Y, Amante FH, Randall LM, Haque A, Pellicci DG, et al. Activation of invariant NKT cells exacerbates experimental visceral leishmaniasis. PLoS Pathog. 2008 Feb 15;4(2):e1000028. [PMC free article] [PubMed]
44. Mattner J, Donhauser N, Werner-Felmayer G, Bogdan C. NKT cells mediate organ-specific resistance against Leishmania major infection. Microbes Infect. 2006 Feb;8(2):354–362. [PubMed]
45. Ishikawa H, Hisaeda H, Taniguchi M, Nakayama T, Sakai T, Maekawa Y, et al. CD4(+) v(alpha)14 NKT cells play a crucial role in an early stage of protective immunity against infection with Leishmania major. Int Immunol. 2000 Sep;12(9):1267–1274. [PubMed]
46. Duthie MS, Kahn M, White M, Kapur RP, Kahn SJ. Critical proinflammatory and anti-inflammatory functions of different subsets of CD1d-restricted natural killer T cells during Trypanosoma cruzi infection. Infect Immun. 2005 Jan;73(1):181–192. [PMC free article] [PubMed]
47. Procopio DO, Almeida IC, Torrecilhas AC, Cardoso JE, Teyton L, Travassos LR, et al. Glycosylphosphatidylinositol-anchored mucin-like glycoproteins from Trypanosoma cruzi bind to CD1d but do not elicit dominant innate or adaptive immune responses via the CD1d/NKT cell pathway. J Immunol. 2002 Oct 1;169(7):3926–3933. [PubMed]
48. Faveeuw C, Mallevaey T, Trottein F. Role of natural killer T lymphocytes during helminthic infection. Parasite. 2008 Sep;15(3):384–388. [PubMed]
49. Mallevaey T, Fontaine J, Breuilh L, Paget C, Castro-Keller A, Vendeville C, et al. Invariant and noninvariant natural killer T cells exert opposite regulatory functions on the immune response during murine schistosomiasis. Infect Immun. 2007 May;75(5):2171–2180. [PMC free article] [PubMed]
50. Mallevaey T, Zanetta JP, Faveeuw C, Fontaine J, Maes E, Platt F, et al. Activation of invariant NKT cells by the helminth parasite schistosoma mansoni. J Immunol. 2006 Feb 15;176(4):2476–2485. [PubMed]
51. van der Vliet HJ, von Blomberg BM, Hazenberg MD, Nishi N, Otto SA, van Benthem BH, et al. Selective decrease in circulating V alpha 24+V beta 11+ NKT cells during HIV type 1 infection. J Immunol. 2002 Feb 1;168(3):1490–1495. [PubMed]
52. Moore Coreceptors: implications for HIV pathogenesis and therapy. Science. 1997 [PubMed]
53. Cho S, Knox KS, Kohli LM, He JJ, Exley MA, Wilson SB, et al. Impaired cell surface expression of human CD1d by the formation of an HIV-1 Nef/CD1d complex. Virology. 2005 Jul 5;337(2):242–252. [PubMed]
54. Chen N, McCarthy C, Drakesmith H, Li D, Cerundolo V, McMichael AJ, et al. HIV-1 down-regulates the expression of CD1d via Nef. Eur J Immunol. 2006 Feb;36(2):278–286. [PubMed]
55. van der Vliet HJ, van Vonderen MG, Molling JW, Bontkes HJ, Reijm M, Reiss P, et al. Cutting edge: Rapid recovery of NKT cells upon institution of highly active antiretroviral therapy for HIV-1 infection. J Immunol. 2006 Nov 1;177(9):5775–5778. [PubMed]
56. Huang Y, Chen A, Li X, Chen Z, Zhang W, Song Y, et al. Enhancement of HIV DNA vaccine immunogenicity by the NKT cell ligand, alpha-galactosylceramide. Vaccine. 2008 Mar 28;26(15):1807–1816. [PubMed]
57. Exley MA, Bigley NJ, Cheng O, Tahir SM, Smiley ST, Carter QL, et al. CD1d-reactive T-cell activation leads to amelioration of disease caused by diabetogenic encephalomyocarditis virus. J Leukoc Biol. 2001 May;69(5):713–718. [PubMed]
58. Exley MA, Bigley NJ, Cheng O, Shaulov A, Tahir SM, Carter QL, et al. Innate immune response to encephalomyocarditis virus infection mediated by CD1d. Immunology. 2003 Dec;110(4):519–526. [PubMed]
59. Ilyinskii PO, Wang R, Balk SP, Exley MA. CD1d mediates T-cell-dependent resistance to secondary infection with encephalomyocarditis virus (EMCV) in vitro and immune response to EMCV infection in vivo. J Virol. 2006 Jul;80(14):7146–7158. [PMC free article] [PubMed]
60. Hobbs JA, Cho S, Roberts TJ, Sriram V, Zhang J, Xu M, et al. Selective loss of natural killer T cells by apoptosis following infection with lymphocytic choriomeningitis virus. J Virol. 2001 Nov;75(22):10746–10754. [PMC free article] [PubMed]
61. Roberts TJ, Lin Y, Spence PM, Van Kaer L, Brutkiewicz RR. CD1d1-dependent control of the magnitude of an acute antiviral immune response. J Immunol. 2004 Mar 15;172(6):3454–3461. [PubMed]
62. Shimamura M, Huang YY, Suda Y, Kusumoto S, Sato K, Grusby MJ, et al. Positive selection of NKT cells by CD1(+), CD11c(+) non-lymphoid cells residing in the extrathymic organs. Eur J Immunol. 1999 Dec;29(12):3962–3970. [PubMed]
63. Lin Y, Roberts TJ, Wang CR, Cho S, Brutkiewicz RR. Long-term loss of canonical NKT cells following an acute virus infection. Eur J Immunol. 2005 Mar;35(3):879–889. [PubMed]
64. Youn HJ, Ko SY, Lee KA, Ko HJ, Lee YS, Fujihashi K, et al. A single intranasal immunization with inactivated influenza virus and alpha-galactosylceramide induces long-term protective immunity without redirecting antigen to the central nervous system. Vaccine. 2007 Jul 9;25(28):5189–5198. [PubMed]
65. Ko SY, Ko HJ, Chang WS, Park SH, Kweon MN, Kang CY. alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor. J Immunol. 2005 Sep 1;175(5):3309–3317. [PubMed]
66. Kamijuku Mechanism of NKT cell activation by intranasal coadministration of alpha-galactosylceramide, which can induce cross-protection against influenza viruses. Mucosal Immunology. 2008 [PubMed]
67. Ho LP, Denney L, Luhn K, Teoh D, Clelland C, McMichael AJ. Activation of invariant NKT cells enhances the innate immune response and improves the disease course in influenza A virus infection. Eur J Immunol. 2008 Jul;38(7):1913–1922. [PubMed]
68. De Santo C, Salio M, Masri SH, Lee LY, Dong T, Speak AO. Invariant NKT cells reduce the immunosuppressive activity of influenza A virus-induced myeloid-derived suppressor cells in mice and humans. J Clin Invest. 2008 Nov 13; [PMC free article] [PubMed]
69. Durbin JE, Durbin RK. Respiratory syncytial virus-induced immunoprotection and immunopathology. Viral Immunol. 2004;17(3):370–380. [PubMed]
70. Krishnan S, Halonen M, Welliver RC. Innate immune responses in respiratory syncytial virus infections. Viral Immunol. 2004;17(2):220–233. [PubMed]
71. Tripp RA, Moore D, Barskey At, Jones L, Moscatiello C, Keyserling H, et al. Peripheral blood mononuclear cells from infants hospitalized because of respiratory syncytial virus infection express T helper-1 and T helper-2 cytokines and CC chemokine messenger RNA. J Infect Dis. 2002 May 15;185(10):1388–1394. [PubMed]
72. Johnson TR, Hong S, Van Kaer L, Koezuka Y, Graham BS. NK T cells contribute to expansion of CD8(+) T cells and amplification of antiviral immune responses to respiratory syncytial virus. J Virol. 2002 May;76(9):4294–4303. [PMC free article] [PubMed]
73. Furlan R, Bergami A, Cantarella D, Brambilla E, Taniguchi M, Dellabona P, et al. Activation of invariant NKT cells by alphaGalCer administration protects mice from MOG35–55-induced EAE: critical roles for administration route and IFN-gamma. Eur J Immunol. 2003 Jul;33(7):1830–1838. [PubMed]
74. Jahng AW, Maricic I, Pedersen B, Burdin N, Naidenko O, Kronenberg M, et al. Activation of natural killer T cells potentiates or prevents experimental autoimmune encephalomyelitis. J Exp Med. 2001 Dec 17;194(12):1789–1799. [PMC free article] [PubMed]
75. Colucci F, Samson SI, DeKoter RP, Lantz O, Singh H, Di Santo JP. Differential requirement for the transcription factor PU.1 in the generation of natural killer cells versus B and T cells. Blood. 2001 May 1;97(9):2625–2632. [PubMed]
76. Teige A, Teige I, Lavasani S, Bockermann R, Mondoc E, Holmdahl R, et al. CD1-dependent regulation of chronic central nervous system inflammation in experimental autoimmune encephalomyelitis. J Immunol. 2004 Jan 1;172(1):186–194. [PubMed]
77. Mars LT, Laloux V, Goude K, Desbois S, Saoudi A, Van Kaer L, et al. Cutting edge: V alpha 14-J alpha 281 NKT cells naturally regulate experimental autoimmune encephalomyelitis in nonobese diabetic mice. J Immunol. 2002 Jun 15;168(12):6007–6011. [PubMed]
78. Tsunoda I, Tanaka T, Fujinami RS. Regulatory role of CD1d in neurotropic virus infection. J Virol. 2008 Oct;82(20):10279–10289. [PMC free article] [PubMed]
79. Grubor-Bauk B, Simmons A, Mayrhofer G, Speck PG. Impaired clearance of herpes simplex virus type 1 from mice lacking CD1d or NKT cells expressing the semivariant V alpha 14-J alpha 281 TCR. J Immunol. 2003 Feb 1;170(3):1430–1434. [PubMed]
80. Grubor-Bauk B, Arthur JL, Mayrhofer G. Importance of NKT cells in resistance to herpes simplex virus, fate of virus infected neurons and level of latency in mice. J Virol. 2008 Jul 9; [PMC free article] [PubMed]
81. Cornish AL, Keating R, Kyparissoudis K, Smyth MJ, Carbone FR, Godfrey DI. NKT cells are not critical for HSV-1 disease resolution. Immunol Cell Biol. 2006 Feb;84(1):13–19. [PubMed]
82. Yuan W, Dasgupta A, Cresswell P. Herpes simplex virus evades natural killer T cell recognition by suppressing CD1d recycling. Nat Immunol. 2006 Aug;7(8):835–842. [PubMed]
83. Raftery MJ, Winau F, Kaufmann SHE, Schaible UE, Schonrich G. CD1 Antigen Presentation by Human Dendritic Cells as a Target for Herpes Simplex Virus Immune Evasion. J Immunol. 2006 November 1;177(9):6207–6214. 2006. [PubMed]
84. Sanchez DJ, Gumperz JE, Ganem D. Regulation of CD1d expression and function by a herpesvirus infection. J Clin Invest. 2005 May;115(5):1369–1378. [PMC free article] [PubMed]
85. Lussier G. Murine cytomegalovirus (MCMV) Adv Vet Sci Comp Med. 1975;19:223–247. [PubMed]
86. Dokun AO, Kim S, Smith HR, Kang HS, Chu DT, Yokoyama WM. Specific and nonspecific NK cell activation during virus infection. Nat Immunol. 2001 Oct;2(10):951–956. [PubMed]
87. French AR, Sjolin H, Kim S, Koka R, Yang L, Young DA, et al. DAP12 signaling directly augments proproliferative cytokine stimulation of NK cells during viral infections. J Immunol. 2006 Oct 15;177(8):4981–4990. [PubMed]
88. Daniels KA, Devora G, Lai WC, O'Donnell CL, Bennett M, Welsh RM. Murine cytomegalovirus is regulated by a discrete subset of natural killer cells reactive with monoclonal antibody to Ly49H. J Exp Med. 2001 Jul 2;194(1):29–44. [PMC free article] [PubMed]
89. Lee SH, Girard S, Macina D, Busa M, Zafer A, Belouchi A, et al. Susceptibility to mouse cytomegalovirus is associated with deletion of an activating natural killer cell receptor of the C-type lectin superfamily. Nat Genet. 2001;28(1):42–45. [PubMed]
90. Brown MG, Dokun AO, Heusel JW, Smith HR, Beckman DL, Blattenberger EA, et al. Vital involvement of a natural killer cell activation receptor in resistance to viral infection. Science. 2001 May 4;292(5518):934–937. [PubMed]
91. Arase H, Mocarski ES, Campbell AE, Hill AB, Lanier LL. Direct recognition of cytomegalovirus by activating and inhibitory NK cell receptors. Science. 2002 May 17;296(5571):1323–1326. [PubMed]
92. Voigt V, Forbes CA, Tonkin JN, Degli-Esposti MA, Smith HR, Yokoyama WM, et al. Murine cytomegalovirus m157 mutation and variation leads to immune evasion of natural killer cells. Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13483–13488. [PubMed]
93. Sjolin H, Tomasello E, Mousavi-Jazi M, Bartolazzi A, Karre K, Vivier E, et al. Pivotal role of KARAP/DAP12 adaptor molecule in the natural killer cell-mediated resistance to murine cytomegalovirus infection. J Exp Med. 2002 Apr 1;195(7):825–834. [PMC free article] [PubMed]
94. Desrosiers MP, Kielczewska A, Loredo-Osti JC, Adam SG, Makrigiannis AP, Lemieux S, et al. Epistasis between mouse Klra and major histocompatibility complex class I loci is associated with a new mechanism of natural killer cell-mediated innate resistance to cytomegalovirus infection. Nat Genet. 2005 Jun;37(6):593–599. [PMC free article] [PubMed]
95. Andrews DM, Scalzo AA, Yokoyama WM, Smyth MJ, Degli-Esposti MA. Functional interactions between dendritic cells and NK cells during viral infection. Nat Immunol. 2003 Feb;4(2):175–181. [PubMed]
96. van Dommelen SL, Tabarias HA, Smyth MJ, Degli-Esposti MA. Activation of natural killer (NK) T cells during murine cytomegalovirus infection enhances the antiviral response mediated by NK cells. J Virol. 2003 Feb;77(3):1877–1884. [PMC free article] [PubMed]
97. Wesley JD, Tessmer MS, Chaukos D, Brossay L. NK cell-like behavior of Valpha14i NK T cells during MCMV infection. PLoS Pathog. 2008 Jul;4(7):e1000106. [PMC free article] [PubMed]
98. Tyznik Cutting edge: the mechanism of invariant NKT cell responses to viral danger signals. J Immunol. 2008 [PMC free article] [PubMed]
99. Kakimi K, Guidotti LG, Koezuka Y, Chisari FV. Natural killer T cell activation inhibits hepatitis B virus replication in vivo. J Exp Med. 2000 Oct 2;192(7):921–930. [PMC free article] [PubMed]
100. Ito H, Ando K, Ishikawa T, Nakayama T, Taniguchi M, Saito K, et al. Role of V{alpha}14+ NKT cells in the development of Hepatitis B virus-specific CTL: activation of V{alpha}14+ NKT cells promotes the breakage of CTL tolerance. Int Immunol. 2008 July 1;20(7):869–879. 2008. [PubMed]
101. Dong Z, Zhang J, Sun R, Wei H, Tian Z. Impairment of liver regeneration correlates with activated hepatic NKT cells in HBV transgenic mice. Hepatology. 2007 Jun;45(6):1400–1412. [PubMed]
102. Parekh VV, Wilson MT, Olivares-Villagomez D, Singh AK, Wu L, Wang CR, et al. Glycolipid antigen induces long-term natural killer T cell anergy in mice. J Clin Invest. 2005 Sep;115(9):2572–2583. [PMC free article] [PubMed]
103. Sullivan BA, Kronenberg M. Activation or anergy: NKT cells are stunned by alpha-galactosylceramide. J Clin Invest. 2005 Sep;115(9):2328–2329. [PMC free article] [PubMed]
104. Kim S, Lalani S, Parekh VV, Vincent TL, Wu L, Van Kaer L. Impact of bacteria on the phenotype, functions, and therapeutic activities of invariant NKT cells in mice. J Clin Invest. 2008 Jun;118(6):2301–2315. [PMC free article] [PubMed]
105. Martinic MM, von Herrath MG. Novel strategies to eliminate persistent viral infections. Trends Immunol. 2008 Mar;29(3):116–124. [PubMed]