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Cancer Res. Author manuscript; available in PMC Aug 16, 2010.
Published in final edited form as:
PMCID: PMC2921769
NIHMSID: NIHMS225836
Malignant Progression and Blockade of Angiogenesis in a Murine Transgenic Model of Neuroblastoma
Louis Chesler,1,5 David D. Goldenberg,2 Isha T. Seales,2 Ronit Satchi-Fainaro,7 Matt Grimmer,1 Rodney Collins,6 Chris Struett,2 Kim N. Nguyen,2 Grace Kim,6 Tarik Tihan,6 Yun Bao,2 Rolf A. Brekken,8 Gabriele Bergers,3,4,5 Judah Folkman,9 and William A. Weiss1,2,3,4,5
1 Department of Pediatrics, University of California-San Francisco Medical School, San Francisco, California
2 Department of Neurology, University of California-San Francisco Medical School, San Francisco, California
3 Department of Neurological Surgery, University of California-San Francisco Medical School, San Francisco, California
4 Brain Tumor Research Center, University of California-San Francisco Medical School, San Francisco, California
5 Comprehensive Cancer Center, University of California-San Francisco Medical School, San Francisco, California
6 Department of Pathology, University of California-San Francisco Medical School, San Francisco, California
7 Department of Physiology and Pharmacology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
8 Departments of Surgery and Pharmacology, Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas, Texas
9 Departments of Surgery and Cellular Biology, Harvard Medical School and Children’s Hospital, Boston, Massachusetts
Requests for reprints: William A. Weiss, Department of Neurology, University of California-San Francisco, 533 Parnassus Avenue, Room U441O, San Francisco, CA 94143. Phone: 415-502-1694; Fax: 415-476-0133; weiss/at/cgl.ucsf.edu
L. Chesler and D.D. Goldenberg contributed equally to this work.
Targeted expression of MYCN to the neural crest [under control of the rat tyrosine hydroxylase (TH) promoter] causes neuroblastoma in transgenic mice (TH-MYCN) and is a well-established model for this disease. Because high levels of MYCN are associated with enhanced tumor angiogenesis and poor clinical outcome in neuroblastoma, we serially characterized malignant progression, angiogenesis, and sensitivity to angiogenic blockade in tumors from these animals. Tumor cells were proliferative, secreted high levels of the angiogenic ligand vascular endothelial growth factor (VEGF), and recruited a complex vasculature expressing the angiogenic markers VEGF-R2, α-SMA, and matrix metalloproteinases MMP-2 and MMP-9, all of which are also expressed in human disease. Treatment of established murine tumors with the angiogenesis inhibitor TNP-470 caused near-complete ablation, with reduced proliferation, enhanced apoptosis, and vasculature disruption. Because TNP-470 has been associated with neurotoxicity, we tested the recently described water-soluble HPMA copolymer–TNP-470 conjugate (caplostatin), which showed comparable efficacy and was well tolerated without weight loss or neurotoxicity as measured by rotarod testing. This study highlights the importance of angiogenesis inhibition in a spontaneous murine tumor with native tumor–microenvironment interactions, validates the use of mice transgenic for TH-MYCN as a model for therapy in this common pediatric tumor, and supports further clinical development of caplostatin as an antiangiogenic therapy in childhood neuroblastoma.
Neuroblastoma is the most common extracranial solid tumor of childhood (1, 2). Approximately one third of tumors show amplification of MYCN, a genetic abnormality closely associated with poor outcome (1). High-risk tumors amplified for MYCN typically show a paucity of stromal elements, compared with tumors diploid for MYCN, which are stroma rich and less aggressive (1, 3). Although these observations suggest that cells in the microenvironment influence the behavior of transformed neuroblasts, the factors contributing to this process in neuroblastoma remain poorly understood.
The acquisition of tumor-derived vasculature is a critical example of tumor-microenvironment interactions in cancer (4). Potential mechanisms through which neuroblast-specific expression of MYCN induces angiogenesis in the surrounding microenvironment include activation of vascular endothelial growth factor (VEGF) and the proangiogenic matrix metalloproteinase-2 (MMP-2) and repression of the angiogenesis inhibitors thrombospondin, activin-A, and angiopoietin, all of which act on endothelial cells (512). These observations suggest that neuroblasts in this embryonal tumor signal to the microenvironment to promote angiogenesis, that Mycn protein itself is critical to angiogenesis in the subset of high-risk tumors that show amplification of MYCN, and that relevant models of tumor-microenvironment interactions are needed to test targeted therapy in general and antiangiogenic therapies in particular in this disease.
We previously generated a model for high-risk, MYCN-amplified neuroblastoma by directing expression of MYCN to the peripheral neural crest of transgenic mice under control of the rat tyrosine hydroxylase (TH) promoter (13). Mice transgenic for TH-MYCN in the 129/SvJ background develop tumors in peripheral neural crest-derived structures and show the typical histopathologic features of human neuroblastoma. Genome-wide screens of tumor tissue revealed localized gains of mouse chromosome 11 (orthologous to gains on human chromosome 17q commonly observed in human tumors), clustered loss of chromosomes 5, 9, and 16 (syntenic to an analogous clustered loss of chromosomes 3p, 4p, and 11q in human tumors), and amplification of the TH-MYCN transgene (1416). Significantly, these mice replicate the microenvironmental architecture characteristic of human MYCN-amplified tumors. Because the effect of MYCN on neuroblasts is highly context specific, interactions between tumor and host-derived stromal components (e.g., infiltrating Schwann cells and host-secreted metalloproteinases) have a major effect on tumor biology and responses to therapy (17). These critical interactions between the tumor and its microenvironment reinforce the importance of species-matched interactions between host and stromal elements.
To develop these mice as a platform for targeted therapy and to exploit species-matched interactions between tumor and microenvironment in this native model, we characterized malignant progression and neovascularization in murine tumors hemizygous for the MYCN transgene. Animals with tumors were divided into three groups: early (group I), intermediate (group II), and advanced (group III). Within this framework, we assessed indices of proliferation, apoptosis, and vascularity and correlated these with the expression of markers known in human tumors to be associated with malignant progression in tumor cells and with angiogenesis in the microenvironment. We next evaluated sensitivity to antiangiogenic therapy. Remarkably, and in contrast with published data using human tumors xenografted into immunocompromised mice (1821), the antiangiogenic fumagillin analogue TNP-470 led to near-complete regression of advanced tumors. The HPMA copolymer–conjugated form of TNP-470 (caplostatin) was equally effective and is an attractive agent for clinical use due to its favorable pharmacokinetics, nontoxic profile, and water solubility. These studies establish the importance of interactions between tumor and microenvironment in a species-matched immunocompetent mouse model for this common peripheral nervous system tumor and suggest that caplostatin should be further tested clinically in the treatment of childhood neuroblastoma.
Tissue preparation
Mice were anesthetized and either lectin perfused or sacrificed without perfusion. Tumor tissues were embedded and flash frozen immediately after excision. Lectin-perfused animals received a retroorbital injection of 1 μL/g of rhodamine-conjugated RCAI lectin (Vector Laboratories). Animals were then perfused with 4% paraformaldehyde (Fisher) at 15 mL/min, followed by further perfusion with PBS at the same rate. Both flash frozen and lectin-perfused tissues were sectioned at 10-μm thickness, mounted and stored at −80°C. All experiments were approved by the University of California-San Francisco Institutional Animal Care and Use Committee.
Immunocytochemical staining
Frozen sections were fixed in 4% paraformaldehyde. Tissues were blocked for 1 h in 0.5% Tween (Fisher), 2% bovine serum albumin (Sigma Chemical Co.), and 5% normal horse or goat serum (Jackson ImmunoResearch, Inc.) and then incubated with primary antisera and dilutions: monoclonal rat anti-mouse CD31 antibody (BD PharMingen, Inc.), 1:500; polyclonal rabbit antisera to all isoforms of VEGF (Neomarker, Inc.), 1:300; polyclonal rabbit antisera to VEGF-R2 (22), 10 μg/mL; polyclonal rabbit anti-human MMP-2 (Chemicon, Inc.), 1:50; monoclonal mouse anti–α-SMA clone 1A4 (Sigma), 1:1,000; polyclonal rabbit antisera to Ki67 (Novocastra, Inc.), 1:500. Secondary antibodies included Cy-2–conjugated donkey anti-rat, Cy-3–conjugated donkey anti-rabbit, or Cy-3 donkey anti-mouse (all from Jackson ImmunoResearch; 1:200).
Quantification of proliferation and apoptosis
Immunofluorescent images were examined using MetaMorph 6.1 (Molecular Devices Corp.). In representative slides from tumors of each group, immunofluorescence for TUNEL, bis-benzimide, or Ki67 was separately quantified in five fields. Results were averaged for tumors of each group. The Student’s t test was used to evaluate statistical significance. Apoptotic cells were identified using the DEADEND Fluorometric TUNEL System (Promega, Inc.). Tissues were sectioned at 4 μm and stained with Gill’s hematoxylin III (American Master Tech Scientific, Inc.) and 1% alcoholic eosin (Sigma). H&E-stained tissues were viewed with an Axioskop 2 FS+, and images were captured with an AxioCam HR (Carl Zeiss MicroImaging, Inc.). Immunofluorescence was viewed and photographed with a Confocal Laser Scanning Microscope 510 NLO (Zeiss).
Reverse transcription-PCR
Tumor tissues were flash frozen and stored at −80°C. Total RNA was extracted using Trizol reagent (Invitrogen, Inc.), and 6 μg of total RNA were reverse transcribed using Superscript II RNaseH H (Invitrogen). Expression of MMP-9 and L-19 (loading control) was assessed after 30 cycles using the primers indicated (Supplementary Fig. S1).
Treatment of mice with TNP-470 and caplostatin
Animals hemizygous for the MYCN transgene with palpable, intermediate group tumors (~60 days of life) were treated three times per week with i.p. injections of TNP-470 (100 mg/kg in 100 μL DMSO) or DMSO alone for 2 weeks (eight animals per group). At sacrifice, tumors were excised, measured, weighed, snapped-frozen for immunoblotting, fixed in 10% buffered formalin for paraffin embedding or fresh-frozen for immunohistochemistry of frozen sections. Mice were weighed daily to monitor for drug toxicity. Weight loss was limited to 15% by institutional protocol. Treatment was terminated at 2 weeks when three animals in the treated group had weight loss above the cutoff (Supplementary Fig. S2). Caplostatin experiments were done similarly at an i.p. TNP-470 equivalent dose of 50 mg/kg dissolved in saline. This dose (50% of the dose used for unconjugated TNP-470 experiments) is similar to dosing of caplostatin used in other animal models.
Evaluation of neurotoxicity
Neurotoxicity was monitored using a rotarod assay, which uses a rotating treadmill (Medical Associates, Inc.), to assess coordination and balance. Before and after 1 and 2 weeks of therapy with caplostatin, mice were first placed on the rotarod for 30 s to permit acclimation. Mice were then replaced on the rotarod at a standard acceleration rate of 3 rotations/min/s. Time on the treadmill (hang time) was recorded for each animal.
Statistical methods
In vitro data are expressed as means ± SDs, and in vivo data as means ± SE. Statistical significance testing used the Student’s t test. For detected differences, the Wilcoxon rank-sum test was used to assess pairwise differences between treatment groups, with P < 0.05 considered to be statistically significant.
Malignant progression in mice transgenic for TH-MYCN
We dissected a cohort of 60 animals hemizygous for the MYCN transgene in strain 129X1/SvJ at ages ranging from 44 to 80 days (Fig. 1A). Four or more animals were analyzed by full necropsy at each time point. In this strain, tumor onset was reproducible and confined to a narrow age window (Fig. 1A). Penetrance for tumors was 65% by 95 days (Fig. 1B). Tumors arising in the mice were classified according to size and degree of regional spread analogous in these two characteristics to the staging of primary human neuroblastoma (23).
Figure 1
Figure 1
Malignant progression of neuroblastoma in hemizygous mice transgenic for TH-MYCN. A, bar graph of animals dissected at specific time points illustrating the incidence of localized, intermediate, and advanced tumors. B, Kaplan Meier analysis shows that (more ...)
Localized tumors (group I) arose between 45 and 55 days of life (Fig. 1C and D), were small and spherical (approximately the size of a kidney), showed no regional organ invasion, and were not easily palpated (analogous to stage I tumors in humans). Intermediate tumors (group II) arose between 55 and 65 days of life and were 1.5 to 2 kidney diameters, adherent to major blood vessels and organs, and easily palpable (analogous to stage II tumors in humans; Fig. 1E and F). Organ margins were negative for tumor by histologic analysis (data not shown). Advanced tumors (group III) arose after 65 days, were grossly visible in the absence of palpation, and, on necropsy, crossed the midline, reminiscent of stage III tumors in humans in size and degree of regional involvement (Fig. 1G and H). Tumor histology was similar in all groups (Fig. 1D, F, and H), revealing a predominance of small, round, blue tumor cells (neuroblasts) in a stroma-poor background, with an abundance of tumor-associated blood vessels of varying caliber. Tumor-associated vessels in group III lesions, in general, were more dilated, with a larger cross-sectional vessel diameter. There was no difference in vascular leakage between group I and group III lesions visible on unperfused H&E-stained tumor sections, except in areas of localized tumor necrosis, which were more readily apparent in group III lesions (Fig. 1H, inset) and were rare in other groups. Local spread to lymph nodes was observed in all groups (Fig. 1I). Whereas gross metastases were rare, microscopic metastases to the liver and kidney were seen in four of four animals with group III tumors (Fig. 1J).
Proliferation, detected by nuclear localization of Ki67 antigen, was high in all groups (Fig. 2A–C). TUNEL staining of apoptotic nuclei (Fig. 2D–F) increased significantly in group III compared with group I (P < 0.001), consistent with the structural findings of areas of increased cell death and focal apoptosis on H&E staining (Fig. 1D, F, and H). There was no statistical difference in TUNEL positivity between group I and II. The increased apoptosis in advanced tumors likely reflects an inability of these lesions to maintain adequate tissue perfusion and oxygen tension, a characteristic common to large solid tumors (24). Levels of MYCN mRNA were assessed by reverse transcription-PCR (RT-PCR) and did not differ among groups (data not shown).
Figure 2
Figure 2
Apoptosis and proliferation as a function of tumor progression. A–F, Ki67-positive tumor cells (A–C; pink) and TUNEL-positive tumor cells (D–F; green). Blue, bisbenzimide nuclear counterstain. Percentages represent positive staining (more ...)
Murine neuroblastoma is highly vascularized
To evaluate vascularity, mice with palpable tumors were analyzed by perfusion fixation with rhodamine-conjugated lectin, followed by immunofluorescent analysis using antisera to platelet-endothelial cell adhesion molecule (CD31). Tumors displayed a rich vasculature (Fig. 2G–J). Rare small vessels stained for CD31 in the absence of rhodamine immunofluorescence (Fig. 2H–J, arrows) indicating nonfunctioning vessels. Although vasculature was plentiful in all tumors (Fig. 3A–C), vessels seemed dilated and more irregular within advanced lesions (Fig. 3A–C). Periendothelial cells were visualized using antisera to smooth muscle actin (α-SMA) and colocalized to the CD31-stained vasculature in all groups (Fig. 3D–F). There were no quantifiable differences in vascular pericyte coverage between group I and group III tumors. Tumor cells in all groups showed strong cytoplasmic immunoreactivity for VEGF ligand (Fig. 3G–I). To assess VEGF-R2 expression in endothelial cells, tumor tissues were stained with antisera against CD31 and VEGF-R2. Colocalization of CD31 and VEGF-R2 immunoreactivity was illustrated in all groups (Fig. 3J–L). Antisera to MMP-2 showed cytoplasmic staining that associated closely with CD31 positive endothelial cells lining the blood vessels (Fig. 3M–O). The immunoreactivity of MMP-9 could not be adequately assessed using commercial antisera. By RT-PCR, expression of MMP-9 mRNA increased in group III tumors, compared with tumors in groups I and II (Supplementary Fig. S1).
Figure 3
Figure 3
Angiogenic factors contributing to murine neuroblastomas. A–C, CD31-positive endothelial cells (green) and bis-benzimide nuclear counterstain (blue). D–F, association of pericytes with endothelial cells is similar in all tumor groups. (more ...)
Efficacy of angiogenesis inhibitors in murine neuroblastoma
The robust vascularity common to both murine and high-risk MYCN-amplified human neuroblastoma led us to test whether angiogenesis inhibitors could show efficacy. We treated established murine tumors using TNP-470, a methionine aminopeptidase-2 inhibitor with defined activity against xenografted human neuroblastoma (1921, 2528). Groups of eight animals with intermediate tumors were treated with TNP-470 (100 mg/kg i.p. thrice weekly) or placebo for 2 weeks, when euthanasia was required due to significant weight loss in treated animals (Supplementary Fig. S2).
Tumors showed a striking response to therapy compared with placebo-treated controls (Fig. 4A and compare B and C). Marked changes were observed in the histologic appearance and immunohistochemical features of tumors treated with TNP-470 (Fig. 4D–K). The characteristic lobular appearance of tumor neuroblasts with surrounding intact blood vessels was replaced by grossly visible hemorrhagic areas with increased apoptosis and necrosis (Fig. 4, compare D and H). On immunohistochemical analysis of paraffin-embedded sections, treated tumors showed reduced staining for the proliferative marker Ki67, except in well-perfused areas surrounding large blood vessels (compare Fig. 4E and I), supporting a role for vascular integrity in maintaining proliferation of these tumors. The degree of apoptosis, assessed by staining of cleaved caspase-3, was greatly increased by TNP-470 treatment (compare Fig. 4F and J), again with sparing of neuroblasts in perivascular locations (arrows). Disruption of the perilobular distribution of small blood vessels was readily apparent (compared by CD31 staining in Fig. 4G and K), consistent with angiogenic blockade effecting small vessels to a greater degree than large vessels. Taken together, these findings imply that TNP-470 targets vascular integrity in established neuroblastoma tumors with a secondary effect on parenchymal neuroblasts, resulting in eradication of tumor parenchyma through apoptosis and necrosis.
Figure 4
Figure 4
Treatment of established murine neuroblastoma tumors with TNP-470. Animals with intermediate tumors were treated with either DMSO or TNP-470 (100 mg/kg i.p. thrice weekly) for 2 wks (eight per group). A, tumor weight was significantly reduced in the TNP-470–treated (more ...)
Significant weight loss requiring euthanasia (>15%) was observed in treated animals (average weight, 21 ± 2 g in treated animals versus 28 ± 3 g average in controls, P < 0.01). Because neurotoxicity and associated weight loss preclude the use of TNP-470 clinically, we next tested HPMA copolymerized TNP-470 (caplostatin), a water-soluble form of TNP-470 that does not cross the blood-brain barrier, and that is better tolerated in preclinical models (29, 30). Caplostatin was also highly effective as a single agent against established tumors (Fig. 5A and compare B and C). No statistically significant weight loss was observed in caplostatin-treated animals (Supplementary Fig. S2). Furthermore, caplostatin-treated animals displayed similar retention time on the rotarod assay compared with control mice (Supplementary Fig. S3), indicative of normal coordination and balance, and abrogation of the neurotoxicity associated with free TNP-470 (29, 30).
Figure 5
Figure 5
Treatment of established murine neuroblastoma tumors with caplostatin (HPMA-copolymerized TNP-470). Treatment as in Fig. 4, with either saline or caplostatin (50 mg/kg TNP-470 equivalent dose, i.p. thrice weekly for 2 wks at 50% of the TNP-470 equivalent (more ...)
Pediatric embryonal tumor formation fails to conform to established paradigms for malignant progression (31). Whereas aggressive tumors of the brain or colon may arise through the sequential acquisition of malignant characteristics (32, 33), low-risk neuroblastoma tumors diploid for MYCN generally do not progress to high-risk disease MYCN-amplified disease (1). In fact, the clinical presentation of neuroblastoma is consistent with a nonlinear model, through which neuroblast progenitors follow one of several distinct pathways to transformation, leading to tumors of low, intermediate, and high-risk, respectively. Amplification of MYCN is associated with advanced disease and is the best-characterized genetic marker of high-risk neuroblastoma (1). The association of MYCN amplification with aggressive disease suggests that transformed neuroblasts fail to respond to differentiating signals, a process normally associated with down-regulation of Mycn (34).
Within the confines of a murine tumor that models MYCN-amplified neuroblastoma in children, we developed a classification that incorporates tumor size and local invasiveness. Tumor cell proliferation did not change between groups and was therefore not incorporated. Both apoptosis and metastases increased as a function of tumor size, as observed in high-risk tumors in children (35). The increased levels of apoptosis in large murine tumors are also observed in other advanced human neoplasms (3638). Collectively, these observations suggest that murine neuroblastoma driven by a TH-MYCN transgene closely models a subset of childhood neuroblastoma amplified for MYCN, leading to similarly aggressive and poorly differentiated malignancies.
Whereas xenograft models for neuroblastoma have been valuable for testing cytotoxic chemotherapy, the species-mismatched microenvironment has potential limitations for evaluation of targeted antiangiogenic agents. Transgenic mouse models have proved useful for stepwise mechanistic analyses of interactions between tumor and vascular compartments, for studying the effects of inhibiting angiogenesis, and for assessing the effect of such interventions on tumor cells (4, 39, 40). In contrast to flank xenograft models, tumors in mice transgenic for TH-MYCN arise in a species-matched immunocompetent host, in comparable locations to those in children with neuroblastoma (suprarenal, abdominal or thoracic paraspinal ganglia), show genetic heterogeneity typical of human disease (1316, 41), and coopt a species-matched vasculature. These variables, as well as the clinical importance of this tumor in children (the most common extracranial solid tumor of childhood), provide a compelling rationale to develop TH-MYCN transgenic mice as a platform for developmental therapeutics in neuroblastoma.
Given the prominence of interactions between MYCN-driven neuroblasts and the neighboring microenvironment, targeted agents that disrupt these interactions hold great potential for therapy. Childhood MYCN-amplified neuroblastoma is typically a vascular tumor, with much of this neovascularization mediated by interactions between MYCN-amplified neuroblasts and the local microenvironment. Perfusion-fixation of murine tumors with rhodamine and CD31 also documents a comparably complex vasculature mirroring that observed in human disease. Expression of VEGF has been reported in primary neuroblasts from human neuroblastoma, whereas expressions of VEGF-R1, VEGF-R2, MMP2, MMP9, and α-SMA are expressed in endothelial and periendothelial cells within the tumor microenvironment (5, 6, 11, 4246). All of these proteins were also expressed in appropriate tumor and stromal elements from mice transgenic for TH-MYCN.
The sensitivity of these tumors to TNP-470 and the HPMA copolymer caplostatin is marked and is more profound than previous reports of the activity of this and other antiangiogenic agents using human xenograft models for neuroblastoma (1921, 2528, 4749). That an antiangiogenic agent affects the progression of established aggressive neuroblastomas bodes well for comparable agents as components of therapy for disease in humans. TNP-470 itself has shown some toxicity in preclinical and clinical trials. Caplostatin abrogates this toxicity (29, 30) and therefore should be an attractive agent for a phase I clinical study in neuroblastoma.
Supplementary Material
Acknowledgments
Grant support: NIH grants R01CA102321, P30HD28825, K08NS053530-01A3, Campini Family Foundation, Thrasher Research Fund, and Samuel Waxman Cancer Research Foundation.
We thank Nadezhda Milshteyn for genotyping, dissections, and for preservation of tumor samples, Jane Gordon for help with confocal microscope, Steven Song for RT-PCR primers, Patrick McQuillen for assistance with Metamorph Software, and Chris Hackett and Anders Persson for critical review of the manuscript.
Footnotes
Note: Supplementary data for this article are available at Cancer Research Online (http://cancerres.aacrjournals.org/).
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