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Eur Heart J. 2010 August; 31(16): 1975–1984.
Published online 2010 July 2. doi:  10.1093/eurheartj/ehq237
PMCID: PMC2921509
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Arterial and aortic valve calcification inversely correlates with osteoporotic bone remodelling: a role for inflammation



Westernized countries face a growing burden of cardiovascular calcification and osteoporosis. Despite its vast clinical significance, the precise nature of this reciprocal relationship remains obscure. We hypothesize that cardiovascular calcification progresses with inflammation and inversely correlates with bone tissue mineral density (TMD).

Methods and results

Arterial, valvular, and bone metabolism were visualized using near-infrared fluorescence (NIRF) molecular imaging agents, targeting macrophages and osteogenesis. We detected significant arterial and aortic valve calcification in apoE−/− mice with or without chronic renal disease (CRD, 30 weeks old; n = 28), correlating with the severity of atherosclerosis. We demonstrated decreases in osteogenic activity in the femurs of apoE−/− mice when compared with WT mice, which was further reduced with CRD. Three-dimensional micro-computed tomography imaging of the cortical and cancellous regions of femurs quantified structural remodelling and reductions in TMD in apoE−/− and CRD apoE−/− mice. We established significant correlations between arterial and valvular calcification and loss of TMD (R2 = 0.67 and 0.71, respectively). Finally, we performed macrophage-targeted molecular imaging to explore a link between inflammation and osteoporosis in vivo. Although macrophage burden, visualized as uptake of NIRF-conjugated iron nanoparticles, was directly related to the degree of arterial and valvular inflammation and calcification, the same method inversely correlated inflammation with TMD (R2 = 0.73; 0.83; 0.75, respectively).


This study provides direct in vivo evidence that in arteries and aortic valves, macrophage burden and calcification associate with each other, whereas inflammation inversely correlates with bone mineralization. Thus, understanding inflammatory signalling mechanisms may offer insight into selective abrogation of divergent calcific phenomena.

Keywords: Aortic valve calcification, Atherosclerosis, Inflammation, Bone mineral density, Molecular imaging


Clinical studies suggest that cardiovascular calcification, atherosclerosis, and chronic renal disease (CRD) are associated with osteoporosis.1,2 Emerging epidemiological evidence shows age-independent correlation between bone mineral density and cardiovascular events.35 In limited mouse studies, atherosclerosis susceptibility corresponds to a reduction in bone mineralization.6,7 Although these observations suggest common underlying mechanisms, the precise nature of the relationship between arterial calcification, calcific aortic valve disease, and bone osteogenesis, as well as reciprocal regulation of these processes, remains unknown. The National Heart, Lung, and Blood Institute Working Group on Calcific Aortic Stenosis has recently underscored the importance of identifying the relationship between calcification of the aortic valve and bone and the reciprocal regulation of these processes.8

Arterial and aortic valve calcification is a significant cause of morbidity and mortality.9,10 Arterial calcification not only weakens vasomotor responses, but also affects atherosclerotic plaque stability. Emerging evidence suggests that atherosclerotic plaques are prone to rupture, particularly in regions of high background stress with microcalcifications located in the thin fibrous cap.1113 In addition, calcification impairs the movement of aortic valve leaflets, causing life-threatening aortic stenosis and heart failure.14 Recent studies suggest that underlying mechanisms of aortic valve calcification resemble those of atherosclerotic arterial calcification, triggered by haemodynamic stress, reactive oxygen species, and inflammatory cues.1417

Cardiovascular calcification was conventionally viewed as an inevitable consequence of ageing, but recent landmark studies have demonstrated that it is a highly regulated process of mineralization, akin to embryonic bone formation.14,18 Indeed, calcified vascular material is often indistinguishable from bone, and its formation involves cellular and molecular signalling processes found in normal osteogenesis.19,20 We have reported that valvular myofibroblast-like cells, due to their plasticity, respond to various stimuli by undergoing activation and sequential phenotypic differentiation. During cardiovascular calcification, macrophage-derived proteinases such as elastolytic cathepsins or metalloproteinases induce the release of biologically active, soluble elastin-derived peptides that may promote the osteogenic differentiation of myofibroblasts or smooth muscle cells (SMC).21 This finding concurs with studies of phenotypic conversion of vascular SMC and valvular myofibroblasts into osteoblastic cells, characterized by the expression of bone-regulating proteins and osteogenic factors (e.g. alkaline phosphatase, osteopontin, osteocalcin, Runx2/Cbfa1, Osterix).16,22,23

Imaging approaches that can spatially resolve and quantify the temporal pro-osteogenic molecular processes in arteries, valves, and bones are limited. Conventional imaging modalities can identify advanced late-stage calcification, but optical molecular imaging can detect tissue mineralization at the earliest stages. The present study tested the hypothesis that arterial and aortic valve calcification is inversely correlated with low bone tissue mineral density (TMD). The specific objective was to quantify the relationship between cardiovascular calcification (arterial and valvular) and long bone remodelling (cortical and trabecular) in established models of atherosclerosis and CRD,1416 using optical molecular imaging and high-resolution 3D micro-computed tomography (CT). In addition, to demonstrate the association between inflammation, ectopic calcification, and osteoporosis, we directly compared macrophage burden and progression of osteogenic changes in each region of the same animals in vivo and ex vivo. Our results on opposing effects of inflammation in soft tissues (cardiovascular organs) and in bone agree with the previous reports on accelerated osteolysis in inflamed bones.24 Our study provides new insight into the relationship between osteoporosis and cardiovascular calcification and suggests shared inflammatory mechanisms of ectopic calcification and bone osteolysis. It also offers a unique model for exploration of in vivo mechanisms regulating early cardiovascular calcification and bone mineral loss.


An expanded Methods section is provided in the Supplementary material online.

Animal protocol

We studied osteogenic changes in carotid arteries, aortic valves, and femur bones obtained from 30-week-old apoE−/− mice (n = 10) and mice with CRD (n = 10), induced by 5/6 nephrectomy. Age-matched wild-type C57/BL6 mice (WT, n = 8) served as controls. At 30 weeks of age, mice underwent intravital microscopy and were euthanized for ex vivo imaging, followed by histology and statistical analyses. In addition to molecular imaging, the femurs of the mice underwent quantitative micro-CT imaging analyses. The Subcommittee on Research Animal Care at Massachusetts General Hospital approved all procedures.

Molecular imaging agents

To image osteogenesis, we used imaging agent (Osteosense680/OS680, VisEn Medical, Inc., Woburn, MA, USA) binding to hydroxyapatite in actively mineralized regions containing osteoblast-like cells in arteries, valves1416 (Supplementary material online, Figure S1A), and bones.25 Macrophage-targeted near-infrared (NIR)-conjugated iron nanoparticle (Supplementary material online, Figure S1B) was used to detect inflammation.1416

Statistical analyses

Data are presented as mean ± standard deviation. Statistical analyses for comparison groups employed one-way ANOVA, followed by the Tukey–Kramer post hoc test performed with GraphPad prism software (version 4.0, GraphPad Software, San Diego, CA, USA). Tests were two-sided and adjusted for variance inequality where appropriate. Linear regression with Pearson's correlation test was used to determine the covariant relationships between data. Normal distribution of the data was confirmed by comparing against expected normal distributions with given means and standard deviation. In all cases, test probability values less than 0.05 were considered statistically significant.


Serum phosphate, creatinine, cystatin C, and cholesterol levels in apoE/− and chronic renal disease apoE−/− mice

Total cholesterol was significantly increased in apoE−/− and CRD apoE−/− mice, when compared with WT mice (467.2 ± 51.1 and 397.1 ± 24.8 mg/dL vs. 56.3 ± 9.8 mg/dL; P < 0.05). In addition, CRD apoE−/− mice had significantly increased serum phosphate, creatinine, and cystatin C levels when compared with apoE−/− and WT mice (phosphate: 11.4 ± 0.9 vs. 6.3 ± 0.4 and 4.7 ± 0.4 mg/dL; P < 0.05; creatinine: 0.6 ± 0.04 vs. 0.4 ± 0.01 and 0.3 ± 0.01 mg/dL; P < 0.05; cystatin C: 0.9 ± 0.01 vs. 0.6 ± 0.2 and 0.3 ± 0.02 µg/mL; P < 0.001). Greater serum phosphate, creatinine, and cystatin C levels suggest that 5/6 nephrectomy induced kidney failure, which in turn promotes inflammation.15

Osteogenic activity in mouse arteries and aortic valves associate with each other and increase with atherosclerosis severity

Fluorescence reflectance imaging was used to map mineralization in aortas of WT, apoE−/, and CRD apoE−/− mice. Strong signals were detected at the levels of aortic valve, aortic root, aortic arch, and abdominal aorta, in apoE−/− and especially CRD apoE−/− mice when compared with WT mice (Supplementary material online, Figure S2). Intravital imaging of carotid arteries in vivo showed more than 47- and 78-fold increases of calcified tissue area (ROI) in apoE−/− and CRD apoE−/− mice, respectively, compared with WT cohort (Figure 1A and B). In addition, up to 100- and 400-fold increases in osteogenic signal intensity (SI) occurred in apoE−/− and CRD apoE−/− mice, respectively, when compared with the WT cohort (Figure 1A and C). Imaging analyses of the aortic valves in WT, apoE−/, and CRD apoE−/− mice revealed that ROI increased 4- and 20-fold in size in apoE−/− and CRD apoE−/− mice, respectively, compared with ROI in WT mice (Figure 1D and E). Osteogenic SI was further significantly increased in aortic valves of apoE−/− and CRD apoE−/− mice (13- and 50-fold, respectively) when compared with WT mice (Figure 1D and F). We performed a correlative analysis, in addition to Pearson's correlation test, to evaluate an association between vascular and valvular calcification. Figure 1G shows a significant correlation of arterial and aortic valve calcific ROI (r = 0.75; P < 0.05). In addition, arterial osteogenic SI significantly correlated with valvular SI (r = 0.67; P < 0.05; Figure 1H). Furthermore, correlative histopathology corroborated our imaging data (Supplementary material online, Figure S3A and B). These imaging and histological results support the association between arterial and aortic valve calcification.

Figure 1
Induction of osteogenesis in carotid arteries and aortic valves of apoE−/− and chronic renal disease apoE−/− mice, detected by intravital fluorescence microscopy. (A) Calcified regions (region of interest) and mineralization ...

Bone mineralization decreases in femurs of atherosclerotic mice and inversely correlates to arterial and aortic valve calcification

Figure 2 shows mineralization in the mid-shaft region of femurs in WT, apoE−/, and CRD apoE−/− mice, detected by molecular imaging ex vivo. We observed significantly decreased osteogenic SI in apoE−/− mice and CRD apoE−/− mice when compared with WT mice and in apoE−/− mice when compared with CRD apoE−/− mice (Figure 2A and B). Micro-CT imaging further showed a decrease in bone TMD in the mid-shaft region of femurs from apoE−/− mice when compared with WT mice (Figure 2CF), which supports our molecular imaging results. Quantitative analyses demonstrated a significant difference in TMD between apoE−/− mice and WT mice and between CRD apoE−/− mice and WT mice (Figure 2E). Albeit not significant (P = 0.057), the difference between CRD apoE−/− mice and WT mice showed a tendency towards decreased cortical/marrow area ratio in CRD apoE−/− mice, suggesting more severe loss of bone structure in the CRD cohort (Figure 2F). Periosteal perimeters were not different between conditions, suggesting that the endosteal dimensions expanded through osteoclast activity. Micro-CT showed a decrease in TMD in the femur heads of apoE−/− mice when compared with WT mice (Figure 2G and H), though bone volume fraction was not significantly changed (Supplementary material online, Figure S4). Further statistical analyses of trabecular microarchitecture showed a significant decrease in trabecular number (Figure 2I) and a significant increase in trabecular spacing (Figure 2J) between CRD apoE−/− mice and WT mice, but there was no significant difference in trabecular thickness between groups (Figure 2K). These results suggest that osteoporotic remodelling may occur as a consequence of calcific cardiovascular disease. To test this, we applied a linear regression analysis comparing arterial and valvular calcification with bone TMD (Figure 3). Tissue mineral density inversely correlates with the arterial and aortic valve regions of calcification (ROI: R2 = 0.86 and 0.34; respectively; Figure 3A and B). Furthermore, TMD is inversely correlated to osteogenic SI in carotid arteries and aortic valves (SI: R2 = 0.67 and 0.72, respectively; Figure 3C and D). These data suggest a strong inverse correlation between cardiovascular calcification and bone mineralization.

Figure 2
Loss of bone mineral density and alterations in microarchitecture in mouse femurs, detected by 3D micro-computed tomography and molecular imaging. (A) Fluorescence microscopy of mid-shaft region of femurs of mice injected with Osteosense680. (B) Quantification ...
Figure 3
Inverse correlation of bone tissue mineral density with arterial and aortic valve calcification. Relationship of bone tissue mineral density to arterial (A) and valve (B) calcified area (region of interest). Relationship of tissue mineral density to arterial ...

Enhanced inflammation correlates with calcification in arteries and aortic valves

Macrophage-targeted molecular imaging agent (CLIO750) showed negligible inflammatory signal in WT arteries, which was substantially increased in apoE−/− and CRD apoE−/− mice (Figure 4A). Quantitative assessment of arterial inflammatory ROI determined a 44- and 95-fold increases in inflammation tissue area in apoE−/− mice and CRD apoE−/− mice when compared with WT mice, which was further confirmed with significant 326- and 718-fold increases in SI (Figure 4B and C). Likewise, the enhanced expression of inflammation was found in the aortic valves of apoE−/− and CRD apoE−/− mice (Figure 4D). Quantitative analyses demonstrated statistically significant increases in inflammatory valvular ROI and SI between WT, apoE−/−, and CRD apoE−/− groups (ROI: 1.5- and 5.7-fold increases, Figure 4E; SI: 2.6- and 11.6-fold increases, Figure 4F). Further, we found a statistically significant correlation between cardiovascular calcification and inflammation in both arteries (ROI: R2 = 0.73; SI: R2 = 0.54; Figure 4G and H) and valves (ROI: R2 = 0.84; SI: R2 = 0.81; Figure 4I and J). Macrophage staining confirmed our molecular imaging findings (Supplementary material online, Figure S5A and B). Overall, these results suggest that the extent of cardiovascular inflammation correlates with the severity of calcification.

Figure 4
Induction of inflammatory signal in carotid arteries and aortic valves of apoE−/− and chronic renal disease apoE−/− mice, detected by intravital fluorescence microscopy. (A) Intravital microscopy detected CLIO750-derived ...

Long bone inflammation correlates inversely with decreased bone mineral signal and associates positively with cardiovascular calcification

Evidence suggests that inflamed or infected bones shift towards an osteolytic environment. To address further the mechanisms for osteoporotic changes, we attempted to link inflammation and TMD in the same mice used for imaging of cardiovascular calcification. Molecular imaging demonstrated robust inflammation signal in the long bones of both apoE−/− mice and CRD apoE−/− mice, whereas WT mice demonstrated virtually no inflammation (Figure 5A). Using the same approach as before, we found 7.6- and 22-fold increases in long bone inflammation (CLIO SI) for apoE−/− mice and CRD apoE−/− mice, when compared with WT mice (Figure 5B). In contrast to the cardiovascular condition, this increased inflammation correlated with decreased bone mineral signal (R2 = 0.75; Figure 6A). These results strongly suggest that the inflammatory responses in long bones contribute to loss of bone mineralization. Finally, we found strong positive correlations in SI between the degree of arterial or valvular inflammation and bone inflammation (R2 = 0.97; Figure 6B and C). These results support our hypothesis that systemic inflammatory signal simultaneously acts to decrease bone mass and increase cardiovascular calcification.

Figure 5
Correlation of long bone inflammation with osteogenic activity. (A) Molecular imaging of inflammation in the long bones of mice injected with CLIO750. (B) Significant increase in bone inflammation in the chronic renal disease apoE−/− mice ...
Figure 6
Relationship between bone osteogenic activity and inflammation. (A) Inverse association of bone osteogenic activity (Osteosense680; signal intensity) with bone inflammation (CLIO750; signal intensity). (B) Positive relationship between arterial inflammation ...


This study establishes direct in vivo evidence that the calcification of arteries and aortic valves in atherosclerosis and CRD correlates inversely with bone tissue mineralization. We demonstrate an association between aortic valve calcification and arterial calcification and that the degree of cardiovascular calcification correlates directly with the loss of bone mineral. The increased inflammatory activity in arteries, aortic valves, and long bones in atherosclerosis and renal failure demonstrate that inflammation at these three locations is related probably via systemic or circulating inflammatory cues. Furthermore, our study reveals that whereas macrophage burden positively correlates with the extent of early cardiovascular calcification, inflammation inversely associates with bone mineralization. These results build on previous studies of the pathogenesis of cardiovascular calcification, in which advanced molecular imaging approaches demonstrated that atherosclerotic and aortic valve calcification share similar risk factors and are induced by similar pro-inflammatory molecular and cellular processes.1416 Our seemingly paradoxical in vivo evidence on inflammation and bone mineralization concur well with previous reports on osteolytic environment in inflamed bones.24

Arterial calcification occurs in two different forms: intimal calcification, typically associated with atherosclerosis, and medial calcification, an ossification process associated with renal failure and diabetes.9 In dysmetabolic patient populations, both types of arterial calcification often develop simultaneously. Intimal calcification increases the risk of plaque rupture, whereas medial calcification increases arterial stiffness and impairs cardiovascular haemodynamics.9 Although the mechanism remains incompletely understood, it is generally agreed that cardiovascular calcification is a more active biological process than previously considered.26 Clinicopathological studies have shown similarity between calcified vascular material and bone material,20 have observed osteoblasts and osteoclast-like cells in the arterial wall,19 and have demonstrated the presence of various bone-regulating proteins in calcified arteries.27 Osteogenic differentiation of vascular SMC or valvular myofibroblasts is likely caused by various mechanisms, including pro-inflammatory cytokines (e.g. IL-6, TNF-α),10 oxidized lipids,17 and microenvironmental and mechanical cues.28 Biochemical imbalances also accelerate calcification. Studies show that excess phosphate levels in CRD accelerate calcification of vascular SMC or valvular myofibroblasts through the phosphate-induced release of matrix vesicles and apoptosis.15,29

Systemic factors associated with CRD, including reduced circulating levels of erythropoietin, have been shown to reduce osteoblast quantity and bone mineral density via Jak-Stat signalling in haematopoietic stem cells.30 Furthermore, the kidneys represent the site of vitamin D activation, and therefore CRD-associated reductions in activated vitamin D may also induce parallel reductions in bone mass. Whether CRD-induced anaemia or activated vitamin D deficiency plays a role in the reduced bone mass or increased cardiovascular calcification remains uncertain.

Our results directly quantified a paradox of simultaneous osteolysis and ectopic calcification in vivo in the same animals. Although both correlate with age in humans, some clinical studies have shown the association of cardiovascular calcification and osteoporosis to be independent of age.3,31 Research has demonstrated that patients with lower bone density and osteoporosis have more severe atherosclerosis.32,33 Dyslipidaemia also may link bone loss with cardiovascular calcification. Pre-clinical studies suggest that hyperlipidaemia reduces bone mineral density34 and promotes arterial and valvular calcification in mice14,16 and that statins reduce calcification.14,35 Moreover, loss of bone mass leads to increased circulating phosphate and calcium and decreased parathyroid hormone, which stimulate the Cbfa1-dependent mineralization of cardiovascular tissue.36 This evidence suggests that osteoporosis may contribute to cardiovascular calcification by adding to a pathological microenvironment that promotes osteogenesis of the arterial wall and aortic valve. During bone resorption, biochemical factors are released into the circulation, contributing to vascular calcification; this observation agrees with studies showing that agents that block bone resorption in animal models also block vascular calcification.37

Our findings strongly suggest in vivo that systemic and local inflammation drives both cardiovascular calcification and bone loss. In general, inflammation causes ‘hardening’ of soft tissue and ‘softening’ of hard tissue,34 but it is unclear whether the pathways are similar. Connections between inflammation and osteolysis were recently established in arthritic and periodontal diseases.24 Pro-inflammatory cytokines such as IL-6 and TNF-α have been shown to increase the osteoclast activity through the NF-κB pathway.38 Osteoprotegerin, normally produced by vascular SMC and osteoblasts, functions as a decoy receptor for the receptor activator of NF-κB ligand RANKL.26 RANKL, a cytokine that induces osteoclast differentiation and activation, stimulates bone resorption, whereas osteoprotegerin neutralizes the binding of RANKL to RANK and prevents bone loss. Osteoprotegerin-deficient mice demonstrated severe bone loss in addition to medial calcifications.39 Taken together, these studies suggest that inflammation in cardiovascular and bone regions acts through the NF-κB–RANKL pathway, but whether this pathway is utilized simultaneously for ectopic calcification and bone loss is unknown.

Figure 7 shows a schematic illustration of our working hypothesis. The presence of atherosclerosis or CRD likely associates with systemic inflammatory signals, as described above. Local inflammation, as detected by macrophage-targeted NIR fluorescence nanoparticles, associates with increased mineral in cardiovascular tissues and decreased mineral in bone, as detected by Osteosense. Potential mediators associated with systemic and local inflammatory processes are shown, as are possible factors arising from bone, which could further influence cardiovascular calcification. Studies are needed to further establish the relationship between cardiovascular calcification and osteoporosis and to dissect the complex mechanisms for reciprocal regulation of these processes.

Figure 7
Schematic presentation of a hypothetical model on the paradoxical relationship between cardiovascular calcification and bone mineral loss, associated with local and systemic inflammation. Atherosclerosis and chronic renal disease-associated systemic inflammatory ...

Unravelling the mechanisms underlying cardiovascular calcification is an important step towards future strategies. Therapeutic agents that selectively increase or inhibit osteogenesis will be much preferred over current systemic approaches such as statin therapy. The identification of high-risk patients at subclinical stages is an equally important task, requiring new imaging modalities or biomarkers targeting calcification.8 Molecular imaging provides appealing new means to identify early-stage calcifying atherosclerotic plaques and aortic valve lesions and offers a powerful tool in personalized preventive cardiovascular medicine.


This study was supported in part by Wijck-Casper-Stam Foundation (J.H.), American Heart Association Scientist Development Grants (0835460N, E.A.; 0830384N, J.B.), NYSTAR CAT Award (J.B., M.R.), Donald W. Reynolds Foundation (R.W., E.A.), Leducq Foundation (07CVD04; E.A., J.B.) and Translational Program of Excellence in Nanotechnology (5-UO1-HL080731; R.W.). Funding to pay the Open Access publication charges for this article was provided by research grants to Dr Aikawa.

Conflict of interest: R.W. reports serving as a consultant for Visen Medical. Other authors report no disclosures.

Supplementary Material

Supplementary Data:


The authors would like to thank Dr Masanori Aikawa for critical reading of the manuscript and Sara Karwacki for excellent editorial assistance.


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