PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of f1000bioLatest ContentReportsReportsReports
 
F1000 Biol Rep. 2009; 1: 2.
Published online 2009 January 21. doi:  10.3410/B1-2
PMCID: PMC2920685

Alternative macrophage activation and the regulation of metabolism

Abstract

Macrophages are white blood cells that have important roles in phagocytosis and immune responses. A series of recent papers reveals that nuclear receptors influence the precise pathway of macrophage phenotype polarization and that these effects protect against insulin resistance and metabolic syndrome, the most important group of diseases facing the industrialized world.

Introduction and context

Obesity and insulin resistance are often accompanied by low-grade systemic inflammation, and these seemingly disparate phenomena are linked by adipose tissue macrophages (ATMs) [1]. Macrophages are derived from circulating monocytes that migrate into target tissues [2]. Histological analysis reveals striking macrophage infiltration into the white adipose tissue (WAT) of obese and insulin-resistant individuals and this is coupled to expression of inflammatory genes [3,4]. The relationship between insulin resistance and macrophage-mediated inflammation is causal and not simply correlative. Mice with disruptions in genes that mediate monocyte infiltration (for example, genes encoding the chemokine MCP-1 and the chemokine receptor CCR2) and the macrophage inflammatory response (for example, genes encoding the cytokine TNFα, the TNF receptor, the kinase JNK, and the NF-κB inhibitor kinase Ikkβ) improved metabolic profiles, and high doses of anti-inflammatory salicylates improve insulin sensitivity in humans [5].

Peroxisome proliferator-activated receptor gamma (PPARγ) is a nuclear receptor that is activated by fatty acids, and recent discoveries have focused attention on its role in the polarization of macrophage phenotype [6]. It is now known that macrophage phenotype varies greatly in a manner that is influenced by local microenvironment: classically activated M1 macrophages are recruited to sites of infection and tissue damage, where they engulf debris and trigger adaptive immune responses, whereas alternatively activated M2 macrophages limit local inflammatory responses and promote tissue repair [7]. PPARγ is best known for its influence on adipocyte development and as the target for insulin-sensitizing drugs, the thiazolidinediones [8]. However, PPARγ also exerts a widespread influence on macrophage biology [8-10]; PPARγ activators repress pro-inflammatory genes, stimulate transcriptional cascades that promote cholesterol export from foam cells (lipid-laden macrophages) in atherosclerotic plaque and inhibit macrophage infiltration into WAT. In 2007, two groups made the remarkable observation that PPARγ is required for M2 macrophage polarization [6,11]. Equally surprisingly, PPARγ-dependent M2 polarization protects against insulin resistance and other aspects of metabolic syndrome. Mice with macrophage-specific PPARγ gene disruptions exhibit increased obesity on high-fat diets and many of the hallmarks of systemic insulin resistance, including altered capacity for glucose uptake and oxidative phosphorylation in skeletal muscle [6,12].

Major recent advances

How does disruption of the PPARγ gene in macrophages influence insulin resistance and obesity? Because M2 polarization inhibits local inflammatory responses, it seems likely that PPARγ-/- macrophages secrete inflammatory cytokines that alter adipocyte function (Figure 1). Accordingly, Odegaard and colleagues find increased expression of inflammatory markers in adipose tissue in the macrophage-specific PPARγ-knockout mice [6]. This effect is coupled to suppression of multiple genes involved in adipocyte function and insulin response, and co-culture experiments confirm that PPARγ-/- macrophages produce secreted factors that limit insulin sensitivity in adipocytes. Overall, changes in adipocyte function are likely to alter secretion of multiple adipocyte hormones that affect the systemic insulin response. In addition, reduced fat storage in adipose tissue depots will probably be coupled to increased accumulation of lipids in liver, skeletal muscle and other locations with known inhibitory effects on insulin sensitivity in these tissues. PPARγ-dependent M2 polarization might also exert direct effects on other tissues: Hevener and colleagues [12] find that hepatic insulin sensitivity varies with the extent of PPARγ-/- macrophage infiltration into liver.

Figure 1.
Monocytes that enter adipose tissue develop into adipose tissue macrophages (ATMs) that can be polarized in two ways: M2 macrophages respond to local Th2 cytokines to limit the inflammatory response, whereas M1 macrophages respond to local pro-inflammatory ...

Two recent studies have highlighted an equally important role for another PPAR subtype (PPARδ) in alternative macrophage activation and insulin resistance [13,14]. PPARδ is highly expressed in macrophages and is implicated in transcriptional repression of atherogenic inflammation [15-17]. It is also known that alternative macrophage activation is associated with fatty acid β-oxidation and oxidative metabolism [18]: classic effects of PPARδ. Both studies confirm that PPARδ is required for expression of M2-specific genes and show that mice with macrophage-specific PPARδ-/- knockouts develop systemic insulin resistance and hepatic steatosis. Results are not in complete agreement; Odegaard et al. [13] find that macrophage-specific PPARδ knockouts mainly affect hepatic insulin sensitivity via influences on polarization of resident liver macrophages (Kupffer cells), whereas Kang et al.[14] observe prominent effects on ATMs, reductions in adipose tissue insulin sensitivity and adipocyte lipolysis. These discrepancies may be related to the nature of the mouse knockout model, but it should be emphasized that they do not detract from the main conclusion; PPARδ protects against insulin resistance via effects on M2 macrophage polarization.

The papers also point towards roles for cross-talk between resident macrophages and host tissues in local inflammation and development of insulin resistance [13,14]. Kang et al. [14] find that adipocytes and hepatocytes produce helper T-cell (Th2)-type cytokines (IL-4 and IL-13) which prime macrophages along the M2 pathway to limit local inflammatory response; in fact, IL-4 enhances PPARδ expression, directly linking IL-4 signaling to the PPARδ pathway. Both groups find that PPARδ-/- macrophages secrete paracrine factors that promote adipocyte and hepatocyte dysfunction in co-culture experiments. These observations raise the interesting possibility that alterations in resident macrophage polarization lead to runaway derangements in tissue/macrophage interactions in MSX. As macrophages acquire pro-inflammatory characteristics they will alter cytokine secretion in surrounding tissues to trigger further inflammatory responses and production of factors that inhibit local and systemic insulin responses.

Future directions

In summary, the fascinating implication of this series of papers is that strategies that promote M2 macrophage polarization will cure systemic insulin resistance and prevent MSX. Much remains to be learned. There are actually multiple M2 macrophage types with incompletely defined functions [7]; it is not clear whether all M2 subtypes protect against insulin resistance and MSX. It is also not clear that M2 macrophages are always beneficial, as they are implicated in tumor inflammation and fibrosis [1]. It is also likely that macrophage nuclear receptors will be attractive targets for drugs that influence macrophage polarization and insulin resistance; PPARγ and PPARδ have proven beneficial effects and about half of the members of the nuclear receptor gene family are expressed in macrophages [19]. Dissecting the actions of nuclear receptors in macrophage biology is likely to become an even more fertile area of research in the next few years.

Abbreviations

ATM
adipose tissue macrophage
CCR2
chemokine receptor 2, IκB, inhibitor of NF-κB
IL
interleukin
IR
insulin resistance
JNK
Jun kinase
MCP-1
monocyte chemoattractant protein 1
MSX
metabolic syndrome/syndrome X
NR
nuclear receptor
PPAR
peroxisome proliferator activated receptor
TNFα
tumor necrosis factor α
TNFR
tumor necrosis factor receptor
WAT
white adipose tissue

Notes

The electronic version of this article is the complete one and can be found at: http://F1000.com/Reports/Biology/content/1/2

Notes

Competing interests

The author declares that he has no competing interests.

References

1. Hotamisligil GS. Inflammation and metabolic disorders. Nature. 2006;444:860–7. doi: 10.1038/nature05485. [PubMed] [Cross Ref] F1000 Factor 9.0 Exceptional
Evaluated by Marc Jeschke 25 Apr 2008
2. Taylor PR, Martinez-Pomares L, Stacey M, Lin HH, Brown GD, Gordon S. Macrophage receptors and immune recognition. Annu Rev Immunol. 2005;23:901–44. doi: 10.1146/annurev.immunol.23.021704.115816. [PubMed] [Cross Ref]
3. Xu H, Barnes GT, Yang Q, Tan G, Yang D, Chou CJ, Sole J, Nichols A, Ross JS, Tartaglia LA, Chen H. Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance. J Clin Invest. 2003;112:1821–30. [PMC free article] [PubMed] F1000 Factor 6.0 Must Read
Evaluated by Giorgio Berton 14 Jan 2004
4. Weisberg SP, McCann D, Desai M, Rosenbaum M, Leibel RL, Ferrante AW., Jr Obesity is associated with macrophage accumulation in adipose tissue. J Clin Invest. 2003;112:1796–8. [PMC free article] [PubMed] F1000 Factor 6.0 Must Read
Evaluated by Giorgio Berton 14 Jan 2004
5. de Luca C, Olefsky JM. Inflammation and insulin resistance. FEBS Lett. 2008;582:97–105. doi: 10.1016/j.febslet.2007.11.057. [PMC free article] [PubMed] [Cross Ref]
6. Odegaard JI, Ricardo-Gonzalez RR, Goforth MH, Morel CR, Subramanian V, Mukundan L, Eagle AR, Vats D, Brombacher F, Ferrante AW, Chawla A. Macrophage-specific PPARgamma controls alternative activation and improves insulin resistance. Nature. 2007;447:1116–20. doi: 10.1038/nature05894. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 9.6 Exceptional
Evaluated by P'ng Loke 7 Jun 2007, Paul Webb 7 Feb 2008
7. Mantovani A, Sica A, Sozzani S, Allavena P, Vecchi A, Locati M. The chemokine system in diverse forms of macrophage activation and polarization. Trends Immunol. 2004;25:677–86. doi: 10.1016/j.it.2004.09.015. [PubMed] [Cross Ref]
8. Tontonoz P, Spiegelman BM. Fat and beyond: the diverse biology of PPARgamma. Annu Rev Biochem. 2008;77:289–312. doi: 10.1146/annurev.biochem.77.061307.091829. [PubMed] [Cross Ref]
9. Valledor AF, Ricote M. Nuclear receptor signaling in macrophages. Biochem Pharmacol. 2004;67:201–12. doi: 10.1016/j.bcp.2003.10.016. [PubMed] [Cross Ref]
10. Zhang L, Chawla A. Role of PPARgamma in macrophage biology and atherosclerosis. Trends Endocrinol Metab. 2004;15:500–5. doi: 10.1016/j.tem.2004.10.006. [PubMed] [Cross Ref]
11. Bouhlel MA, Derudas B, Rigamonti E, Dièvart R, Brozek J, Haulon S, Zawadzki C, Jude B, Torpier G, Marx N, Staels B, Chinetti-Gbaguidi G. PPARgamma activation primes human monocytes into alternative M2 macrophages with anti-inflammatory properties. Cell Metabolism. 2007;6:137–43. doi: 10.1016/j.cmet.2007.06.010. [PubMed] [Cross Ref]
12. Hevener AL, Olefsky JM, Reichart D, Nguyen MT, Bandyopadyhay G, Leung HY, Watt MJ, Benner C, Febbraio MA, Nguyen AK, Folian B, Subramaniam S, Gonzalez FJ, Glass CK, Ricote M. Macrophage PPAR gamma is required for normal skeletal muscle and hepatic insulin sensitivity and full antidiabetic effects of thiazolidinediones. J Clin Invest. 2007;117:1658–69. doi: 10.1172/JCI31561. [PMC free article] [PubMed] [Cross Ref]
13. Odegaard JI, Ricardo-Gonzalez RR, Red Eagle A, Vats D, Morel CR, Goforth MH, Subramanian V, Mukundan L, Ferrante AW, Chawla A. Alternative M2 activation of Kupffer cells by PPARdelta ameliorates obesity-induced insulin resistance. Cell Metabolism. 2008;7:496–507. doi: 10.1016/j.cmet.2008.04.003. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 6.0 Must Read
Evaluated by Paul Webb 2 Jul 2008
14. Kang K, Reilly SM, Karabacak V, Gangl MR, Fitzgerald K, Hatano B, Lee CH. Adipocyte-derived Th2 cytokines and myeloid PPARdelta regulate macrophage polarization and insulin sensitivity. Cell Metabolism. 2008;7:485–95. doi: 10.1016/j.cmet.2008.04.002. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 6.0 Must Read
Evaluated by Paul Webb 2 Jul 2008
15. Lee CH, Chawla A, Urbiztondo N, Liao D, Boisvert WA, Evans RM, Curtiss LK. Transcriptional repression of atherogenic inflammation: modulation by PPARdelta. Science. 2003;302:453–57. doi: 10.1126/science.1087344. [PubMed] [Cross Ref]
16. Barish GD, Atkins AR, Downes M, Olson P, Chong LW, Nelson M, Zou Y, Hwang H, Kang H, Curtiss L, Evans RM, Lee CH. PPARdelta regulates multiple proinflammatory pathways to suppress atherosclerosis. Proc Natl Acad Sci USA. 2008;105:4271–6. doi: 10.1073/pnas.0711875105. [PubMed] [Cross Ref]
17. Takata Y, Liu J, Yin F, Collins AR, Lyon CJ, Lee CH, Atkins AR, Downes M, Barish GD, Evans RM, Hsueh WA, Tangirala RK. PARdelta-mediated antiinflammatory mechanisms inhibit angiotensin II-accelerated atherosclerosis. Proc Natl Acad Sci USA. 2008;105:4277–82. doi: 10.1073/pnas.0708647105. [PubMed] [Cross Ref]
18. Vats D, Mukundan L, Odegaard JI, Zhang L, Smith KL, Morel CR, Wagner RA, Greaves DR, Murray PJ, Chawla A. Oxidative metabolism and PGC-1beta attenuate macrophage-mediated inflammation. Cell Metabolism. 2006;4:13–24. doi: 10.1016/j.cmet.2006.05.011. [PMC free article] [PubMed] [Cross Ref] F1000 Factor 3.0 Recommended
Evaluated by Dominique Langin 25 Oct 2006
19. Barish GD, Downes M, Alaynick WA, Yu RT, Ocampo CB, Bookout AL, Mangelsdorf DJ, Evans RM. A nuclear receptor atlas: macrophage activation. Mol Endocrinol. 2005;19:2466–77. doi: 10.1210/me.2004-0529. [PubMed] [Cross Ref]

Articles from F1000 Biology Reports are provided here courtesy of Faculty of 1000 Ltd