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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurosci. Author manuscript; available in PMC 2011 January 1.
Published in final edited form as:
PMCID: PMC2920134
NIHMSID: NIHMS221264

Visual Arrestin 1 Acts as a Modulator for N-Ethylmaleimide Sensitive Factor in the Photoreceptor Synapse

Abstract

In the G-protein coupled receptor (GPCR) phototransduction cascade, visual Arrestin1 (Arr1) binds to and deactivates phosphorylated light-activated opsins, a process that is critical for effective recovery and normal vision. In this report, we discovered a novel synaptic interaction between Arr1 and N-ethylmaleimide sensitive factor (NSF) that is enhanced in a dark environment when mouse photoreceptors are depolarized and the rate of exocytosis is elevated. In the photoreceptor synapse, NSF functions to sustain a higher rate of exocytosis, in addition to the compensatory endocytosis to retrieve and to recycle vesicle membrane and synaptic proteins. Not only does Arr1 bind to the junction of NSF N-terminal and its first ATPase domains in an ATP-dependent manner in vitro, but Arr1 also enhances both NSF ATPase and NSF disassembly activities. In vivo experiments in mouse retinas with the Arr1 gene knocked out, the expression levels of NSF and other synapse-enriched components, including vesicular glutamate transporter 1 (vGLUT1), excitatory amino acid transporter 5 (EAAT5), and vesicle associated membrane protein 2 (VAMP2), are markedly reduced, which lead to a substantial decrease in the exocytosis rate with FM1-43. Thus, we propose that the Arr1 and NSF interaction is important for modulating normal synaptic function in mouse photoreceptors. This study demonstrates a vital alternative function for Arr1 in the photoreceptor synapse and provides key insights into the potential molecular mechanisms of inherited retinal diseases, such as Oguchi disease and Arr1-associated retinitis pigmentosa.

Keywords: Visual Arrestin, S-antigen, Cone arrestin, β-arrestin, N-ethylmaleimide sensitive factor, SNARE, photoreceptor, synapse, FM1-43

Introduction

Arrestins play established roles in the regulation of G-protein coupled receptor (GPCR) signaling by specifically binding to agonist-activated, phosphorylated receptors, terminating further G-protein activation. There are four distinct members in the arrestin superfamily expressed in vertebrates (Craft and Whitmore, 1995). Arr1 is expressed in both rod and cone photoreceptors, while Arrestin 4 (Arr4, cone or X-arrestin) is expressed in cone photoreceptors (Nikonov et al., 2008). Both visual arrestins are also highly expressed in pinealocytes (Craft et al., 1994), while β-arrestin1 and β-arrestin2 are ubiquitously expressed and mediate the desensitization of β-adrenergic receptors and many other GPCRs (Lefkowitz et al., 1992). Moreover, β-arrestin1 also directs receptors to coated pits for internalization and switches signaling to alternative pathways (Orsini and Benovic, 1998;Gagnon et al., 1998;Lefkowitz and Shenoy, 2005).

Previous work, which includes gene knockouts of mouse Arr1, demonstrated its function in modulating the phototransduction shutoff and recovery in the rod and cone photoreceptor outer segment by binding to light-activated, phosphorylated opsins and quenching the GPCR phototransduction cascade (Wilden et al., 1986;Xu et al., 1997;Nikonov et al., 2008). Furthermore, mutations in the human gene encoding ARR1 lead to either an inherited recessive form of stationary night blindness known as Oguchi disease that is associated with abnormal electroretinograms (ERGs) of reduced a-wave amplitudes and absent scotopic b-waves (Fuchs et al., 1995;Nakazawa et al., 1997), or retinitis pigmentosa (Nakamachi et al., 1998) that leads to retinal degeneration.

Subcellular localization of signaling molecules is vital for their biological function. Constitutively shuttling of signaling proteins and the redistribution of their interactive partners between subcellular compartments are important for the modulation of their activity. With light exposure, Arr1 is translocated to the outer segments in rods and cones and also contributes to light-adaptation; while in dark-adapted photoreceptors, high concentrations of Arr1 localizes to the inner segment, perinuclear region, and synaptic terminals (Broekhuyse and Winkens, 1985;Philp et al., 1987;Whelan and McGinnis, 1988;Arshavsky, 2003;Brown et al., 2010). Based on the photoreceptor synaptic localization in the dark of Arr1 and alternative functions of the β-arrestins in neuronal synapses, we examined potential binding partners of Arr1.

In this study, we report a protein-protein interaction between Arr1 and N-ethylmaleimide sensitive factor (NSF), an ATPase with diverse cellular activities, which is critical for soluble NSF attachment protein receptor (SNARE) complex disassembly in membrane trafficking events, including neurotransmitter release. Arr1 co-immunoprecipitates with NSF and deletion analysis maps the relevant binding site to the junction of two functional domains of NSF. We also provide evidence supporting the interaction of Arr1 with NSF to modulate its ATPase activity and to drive disassembly of the SNARE complex. Finally, we observe that synaptic vesicle recycling in photoreceptors is dramatically decreased and analysis of the photopic electroretinogram (ERG) b-wave also demonstrate a light adaptation defect in Arr1-/- mice (Brown et al., 2010). These cumulative findings demonstrate that normal photoreceptor synaptic function involves the ability of Arr1 to regulate and to enhance the dark-associated activity of NSF such that photoreceptor synaptic vesicle demand is met efficiently in these specialized sensory cells.

Materials and Methods

Animals

Mice were dark-reared in the USC Vivarium following the appropriate established guidelines of the National Institutes of Health and adapted by the Institutional Animal Care and Use Committee of the University of Southern California. Breeding pairs of the Arrestin1 (Arr1-/-) knockout mice (Xu et al., 1997;Burns et al., 2006) and transgenic mouse cone arrestin (mCAR-H) backcrossed to the Arr1-/- (mCAR-Harr1-/-) (Chan et al., 2007) were generously provided by Dr. Jeannie Chen (USC). Colony control (C57Bl/6J and SVJ129 [WT] mixed genetic background) mice were created by breeding heterozygote littermates of the Arr1+/-Arr4+/- knockout mice. Their offspring were verified by PCR genotype analysis for both Arr1+/+ and Arr4+/+ and used as breeders (WT) (Nikonov et al., 2008). All mice were born and maintained in a dark environment. Both male and female mice were used for these experiments.

Antibody pull-down assays and co-immunoprecipitation

To verify the potential physiological interaction of Arr1 with NSF, antibody pull-down assays and co-immunoprecipitation experiments were carried out as previously described (Zhu et al., 2003). The reactions were performed in both light and dark (infrared or dim red light) conditions. Briefly, retinas from WT mice were homogenized in lysis buffer (50mM Tris-HCl pH 8.0, 1% Triton X-100, 150mM NaCl with 1× proteinase inhibitor cocktail (Roche, Indianapolis, IN), sonicated on ice for 30 sec, incubated at 4°C with gentle shaking for 30 min, and centrifuged at 13000g for 15 min. Equal volumes of supernatant were precleared for 1 hr incubation with 50μl of a 50% slurry of protein-G agarose (KPL, Inc). After centrifugation and the Arr1 in the supernatant was immunoprecipitated using 10μl of mouse monoclonal antibody (MAb) D9F2 specific for Arr1 (AA 361-369/PEDPDTAKE). The tubes were incubated at 4°C overnight and then incubated with 50μl protein-G agarose at 4°C for 2 hrs. The affinity purified anti-rabbit mouse cone arrestin (mCar-LUMIj) polyclonal antibodies (PAb) (Zhu et al., 2002) was used as a nonspecific antibody control. The pellets were washed five times with the lysis buffer containing no proteinase inhibitor. The immunoprecipitated proteins were eluted after boiling for 5 min in SDS-PAGE sample buffer and subjected to 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) followed by transfer to polyvinylidene difluoride membrane (PVDF) membrane. Immunoblot analysis was performed using a PAb specific for NSF (1:10,000, Upstate, 07-364), and NSF was visualized using the enhanced luminol-based chemiluminescent (ECL) system (Amersham Biosciences). To confirm equal loading of Arr1, the PVDF membrane was stripped and reprobed to visualize Arr1 using an affinity purified anti-rabbit PAb C10C10 (1:10,000, AA 293-301/RERRGIALD), which was characterized previously (Brown et al., 2010). This antibody is specific for Arr1 and is similar to the results with MAb D9F2.

Isolation of proteins for mass spectrometry analysis

The identification of potential interacting partners for Arr1 was performed by liquid chromatography-tandem mass spectrometry at the USC School of Pharmacy Proteomics Core Facility. Proteins were prepared and analyzed by mass spectrometry using a method similar to that previously described (Gallaher et al., 2006). Briefly, the Arr1 interacting proteins were separated by electrophoresis on 10% SDS-PAGE and the proteins were visualized by Coomassie blue stain. Excised bands were destained and dehydrated and then digested with trypsin at 37°C overnight. The supernatant was collected and analyzed by liquid chromatography-tandem mass spectrometry. Protein identification was carried out with the MS/MS search software Mascot 1.9 (Matrix Science) with confirmatory or complementary analyses with TurboSequest as implemented in the Bioworks Browser 3.2, build 41 (ThermoFinnegan).

Plasmid construction

Mouse cDNA encoding NSF was amplified by PCR from Image Clone: 4506351(Open Biosystems, Huntsville, AL) with oligonucleotide primers sense +NSF-f76 5′-ATGGCGGGCCGGACTATGCA-3′ and antisense -NSF-r2310 5′-TCAATCAAAGTCCAGGGGAC-3′ (NM_008740 mouse NSF complete coding sequence) was subcloned into the PtrcHis-TOPO vector (Invitrogen, Carlsbad, CA). Mouse Arr1, SNAP-25, α-SNAP, VAMP-2 cDNAs were amplified with PCR technology with specific 5′-sense and 3′-anti-sense primers and subcloned into the PtrcHis-TOPO vector after PCR amplification from cDNA from total mRNA isolated from mouse retina. The cDNA encoding the mouse NSF and Syntaxin 4 were subcloned as fragments into the multiple cloning site using restriction endonucleases for EcoRI-XhoI into the pGEX-4T1 vector (GE Healthcare, Piscataway, NJ). The sequences of all constructs were confirmed by DNA sequencing. Recombinant fusion proteins were purified on columns using the Histidine (His) tags with the Bio-Rad Profinia purification system as recommended by the manufacturer.

GST pull-down and in vitro binding assay

To define the functional domains in NSF that interact with Arr1, His6-tagged, NSF-truncated segments of varying lengths (AA residues 1-744, 251-744, 197-744, and 1-205^478-744) and GST-tagged NSF1-250 and NSF1-197 were constructed. GST-Arr1 proteins (3μg) were immobilized on glutathione-agarose beads in 25 mM HEPES-KOH (pH 7.4), 200 mM KCl, 1% Triton X-100, 10% glycerol and 1 mM DTT (buffer A) and then incubated with His6-NSF1-744, 251-744, 197-744 or 1-205^478-744 at 4°C for 1hr. GST-NSF1-250 or GST-NSF1-197 proteins (3μg) were also immobilized on glutathione-agarose beads in buffer A and then incubated with His6-Arr1 at 4°C for 1h. After six washes in buffer A plus 2 mM ATP, 8 mM MgCl2 (buffer B), bound proteins were eluted with 20mM glutathione and detected by immunoblot analysis.

To evaluate the influence of the ATPase state of NSF on its direct interaction with Arr1, GST-tagged Arr1[AA 1-403] (3 μg), or truncated Arr1[AA 1-191], Arr1[AA 1-370] were immobilized on glutathione-agarose beads in buffer A. Beads were washed twice with buffer B, or 2mM ATP-γ-S and 8mM MgCl2 in the presence of 1% BSA and incubated with 3μg His6-tagged NSF at 4°C for 1hr. After four washes in buffer B without BSA, bound proteins were eluted with 20 mM glutathione and detected by immunoblot analysis as described above. To determine the effect of the Arr1 binding to NSF-ATPase activity, the same procedure was performed in the presence of 8mM MgCl2, 10mMEDTA and 2mM ATP or ATP-γ-S. Densitometric analysis was conducted using the ImageQuant TL software (Amersham Biosciences).

Quantitative real time polymerase chain reaction technology (RT-PCR)

Total RNA was prepared from dark-adapted (24 hrs) and light-adapted (1 hr) frozen retinas using Trizol reagent (Invitrogen, Carlsbad, CA). The cDNA made from 0.5μg total retina RNA was prepared using a reverse transcription system from Invitrogen with oligo(dT)20. Each quantitative RT-PCR reaction was set up in a final volume of 25μl containing 12.5μl SYBR Green from Superarray (Frederick, MD). Reactions were done in triplicate on 96-well plates and quantified (LightCycler 480 Real-Time PCR System; Roche). Data analysis was performed using the Light-Cycler Software Version LCS480 1.2.0. The housekeeping gene, mouse glyceraldehyde-3-phosphate dehydrogenase (mGAPDH), was used as the reference to normalize the expression levels of the NSF, VAMP2, EAAT5 and vGLUT1 transcripts. Values for RTPCR for retinas from light-adapted WT mice were set to 1. Quantitative RTPCR primer pair sequences, sense/forward (+/f) and antisense / reverse (-/r) (gene name, accession number; nucleotide numbers):

  • +mGAPDH-f148-5′-ACCCCTTCATTGACCTCAACTACATGG-3′(NM_008084;148-174)
  • -mGAPDH-r303-5′-ATTTGATGTTAGTGGGGTCTCGCTCCT-3′(NM_008084; 277-303)
  • +qNSF-f2158-5′-GCTCAGCAAGTCAAAGGGAA-3′ (NM_008740; 2158-2177)
  • -qNSF-r2248-5′-GGTACTCAGGATCCATCTGC-3′ (NM_008740; 2229-2248)
  • +qVAMP2-f213-5′-GTGGATGAGGTGGTGGACAT-3′ (NM_009497; 213-232)
  • -qVAMP2-r348-5′-GCTTGGCTGCACTTGTTTCAA-3′ (NM_009497; 328-348)
  • +vGLUT1-f1532-5′-GTGCAATGACCAAGCACAAG-3′ (NM_182993; 1532-1551)
  • -vGLUT1-r1601-5′-TAGTGCACCAGGGAGGCTAT-3′ (NM_182993; 1582-1601)
  • +EAAT5- f1250-5′-GCTCTGCTCATTGCGTTG-3′ (NM_146255; 1250-1267)
  • -EAAT5- r1317-5′-AGCAGGCACTTGAAGGTGAT-3′ (NM_146255; 1298-1317)

Immunoblot Analysis

Protein extracts from dark-adapted (24 hrs) and light-adapted (1 hr) WT or Arr1-/- mouse retina were prepared using NP-40 lysis buffer (50mM Tris-HCl pH7.6, 150 mM NaCl, 1% NP-40 and 1mM EDTA). The protein concentrations were determined using the bicinchoninic acid (BCA) protein assay kit (Pierce). 20μg of extracts were separated on 12% SDS PAGE and transferred to PVDF membrane. After one hr blocking with 5% nonfat milk, the membranes were incubated in different primary antibody (anti-NSF PAb (1:2500; Abcam), Anti-EAAT5 PAb (1:5000; a gift from Dr. David Pow (University of Queensland, Queensland, Australia) anti-vGLUT1 MAb (1:2000; Millipore), anti-VAMP2 MAb (1:5000; Synaptic System), anti-SNAP-25 PAb (1:3000; Abcam)) at 4°C overnight. After washing, the blots were then incubated with appropriate anti-horseradish peroxidase conjugated secondary antibodies (1:10000; Bio-Rad) at room temperature for 1 hr. The proteins on the membranes were detected using the enhanced chemiluminescence system (ECL; Amersham Biosciences). The blots were also probed with mouse glyceraldehydes-3-phophate dehydrogenase (GAPDH) as an internal loading control. Densitometric analysis was conducted using the ImageQuant TL software (Amersham Biosciences). Each experiment was repeated at least three times with independent retinal samples from different groups of mice.

Immunohistochemistry

To further verify the potential physiological relevance of the in vitro interaction between Arr1 and NSF, we performed indirect fluorescent dual immunohistochemical localization as described (Zhu et al., 2003). Briefly, the eyes were enucleated under infrared or light conditions, the cornea was removed, and the eyes were immediately immersed in 4% (w/v) paraformaldehyde (PFA) in 0.1M PBS for 2 hrs at room temperature. Eyes were rinsed in PBS, pH7.4 and cryoprotected in 30% sucrose-PBS solution at 4°C overnight, and then embedded in ornithine carbamyl transferase (OCT; Tissue-Tek, Elkhart, IN). Sections (7μm) of the retina were cut through the optic nerve with a cryostat, and retina sections were washed in 0.1M PBS, blocked in blocking buffer (1% BSA, 1% NGS, 1% Triton X-100 in 1XPBS), and incubated with anti-mouse MAb D9F2 (1:20,000) for Arr1 and anti-rabbit PAb (1:2,500) for NSF at 4°C overnight. To visualize binding of the primary antibodies, sections were incubated in secondary antibody conjugated to Alexa Fluor 488 or 568, respectively (1:500, Invitrogen) and TOPRO-3 (1:2500, Invitrogen) nuclear staining for 1 hr at room temperature. Samples stained without either or both of the primary antibodies were included as controls to ensure the dual-staining pattern results were reliable (data not shown). The sections were visualized and photographed with a Zeiss confocal laser-scanning microscope (Carl Zeiss, Inc).

Cell culture and transfection

COS-7 cells were cultured in Dulbecco's modified Eagle's medium (Invitrogen) supplemented with 10% fetal bovine serum and antibiotics and maintained at 37°C in a humidified atmosphere with 5% CO2 as previously described (Zhu et al., 2003). The plasmids of interest were transfected into COS-7 cells using Fugene Transfection Reagent according to the manufacturer's instruction (Roche). COS-7 cells were co-transfected with plasmids encoding empty vector, pcDNA4/HisMax-Arr1, pcDNA3.1/GFP-NSF or pcDNA4/HisMax-Arr1 plus pcDNA3.1/GFP-NSF for 48 hrs. Cell were washed in PBS and fixed in freshly prepared 4% paraformaldehyde (PFA). Fixed cells were permeabilized and blocked with 0.1% Trixon-X100 and 2.5% normal goat serum in PBS for 30 min at room temperature and then incubated with primary and secondary antibodies diluted in the same buffer for 1 hr at room temperature. After three washes with PBS, the cells were mounted and viewed under a Zeiss confocal laser-scanning microscope.

NSF ATPase activity assay

The basal ATPase activity of NSF was measured by a colorimetric assay (Huynh et al., 2004). Recombinant NSF (0.2μg/μl) was pretreated with 10mM N-ethylmaleimide (NEM), a NSF inhibitor, as a negative control or with increasing concentrations of recombinant Arr1 protein for 10 min at 37 °C. ATPase reaction buffer (25mM Tris-HCl at pH 9.0, 100mM KCl, 0.65mM β-mercaptoethanol, 2mM MgCl2 and 10% glycerol, 10mM ATP) was added to the mixture. The release of inorganic phosphate was measured by adding Biomol Green (Biomol, Plymouth Meeting, PA) and the absorbance at 620 nm was determined on a Benchmark Plus microplate reader (Bio-Rad, Los Angeles, CA). Corrections were made for minor ATPase contaminants, non-enzymatic hydrolysis of ATP and preexisting phosphate in protein samples by subtracting the NEM-treated control values from those obtained at 37°C.

NSF disassembly activity assay

The disassembly activity of NSF was measured by a co-precipitation assay as described previously (Matsushita et al., 2003). Recombinant His6-NSF (0.1μg/μl) was pretreated with buffer or with increasing concentration of recombinant Arr1 protein for 10 min at 37°C. Immobilized GST-Syntaxin 4 (0.1μg/μl) on glutathione-agarose beads was incubated with recombinant His6-α-SNAP (0.1μg/μl) and SNARE polypeptide (0.1μg/μl each of VAMP-2 and SNAP-25) at 4°C for 1hr. The beads were then washed three times with binding/wash buffer (4mM HEPES, pH 7.4, 0.1M NaCl, 1mM EDTA, 3.5mM CaCl2, 3.5mM MgCl2, and 0.5% Triton X-100). The mixture of NSF with increasing concentration of Arr1 was added to the beads and then incubated in binding/wash buffer with 2.5 mM ATP/5mM MgCl2 for 30 min at 4°C with rotation. The beads were washed with binding/wash buffer six times, mixed with SDS-PAGE sample buffer at 60°C for 3 min, resolved on 12% SDS-PAGE, transferred to PVDF, and then analyzed with specific antibodies on immunoblots.

Evaluation of exocytosis rate using FM1- 43 staining

The procedure for activity-dependent staining with FM1-43 was performed as described previously (Miller et al., 2001;Caicedo et al., 2005). The eyecup preparations from WT and Arr1-/- mice after 1 hr of light-adaption and immersed in mammalian Ringer's solution. The eyecups were superfused with depolarizing Ringer's solution containing 10μM fixable FM1-43 (Invitrogen) with or without 25mM K+ for 30 min. After incubation, preparations were washed 3 times with Ringer's solution containing 5mM Co2+ for 5 min to minimize dye release through calcium-dependent exocytosis and to eliminate background staining. Eyecups were fixed in 4% PFA for 1 hr, cryoprotected in 30% sucrose, and cut on a cryostat. FM1-43 staining was visualized and photographed with a Zeiss confocal laser-scanning microscope.

Electroretinography (ERG)

Photopic, cone-driven ERG responses were recorded from mice in the presence of a steady white background light to suppress the rod response, as previously described (Brown et al., 2010). In this current study, the background light was turned on, and after 1 min of light adaptation, a single maximum intensity flash was delivered and averaged every 2 min until 15 min of recording. The amplitudes from at least 8 mice in each group were averaged and two-way ANOVA with Bonferroni post-tests were performed on the data at each time point during recording.

Statistical analysis

Analysis of statistical significance was determined using two-tailed Student's t test and one-way ANOVA followed by Bonferroni's multiple comparison test. Data are presented as means ± standard error of the mean (SEM). In all cases, p<0.05 denotes statistical significance.

Results

NSF is an interacting partner for Arr1 in the photoreceptor synapse

To investigate potential physiologically relevant photoreceptor synaptic partners of Arr1, we performed a pull-down immunoprecipitation assay from mouse colony control wild type (WT) mouse retina homogenates with an Arr1-specific MAb D9F2, followed by SDS-PAGE separation and analysis. Among the major bands identified by mass spectrometry, the major band identified was N-ethylmaleimide sensitive factor (NSF) (NP_032766) (For details, Supplemental Fig.1a). Protein database search using Mascot analysis (Matrix Science) revealed ~22% tryptic peptide coverage with a total Mascot score of 610 of a protein corresponding to NSF (Supplemental Fig.1b). NSF is a well characterized ATPase that is an essential component of various membrane fusions including the exocytosis of synaptic vesicles (Kawasaki et al., 1998;Tolar and Pallanck, 1998;Singh et al., 2004).

To verify the interaction between Arr1 and NSF, we performed additional co-immunoprecipitation assays from retinal homogenates from WT and Arr1 knockout (Arr1-/-) mice. Immunoblot analysis with a rabbit anti-NSF polyclonal antibody (PAb) identified a single band in WT and Arr1-/- retinal homogenates (Fig. 1a), while the anti-Arr1 MAb identified a 48 kDa band corresponding to Arr1 only in the WT, but not in the Arr1-/- retinal homogenates (Fig. 1b). A protein of approximately 76 kDa, corresponding to NSF, was co-immunoprecipitated with Arr1 MAb from the WT but not from the Arr1-/- retina homogenates (Fig.1c), confirming the specific protein-protein interaction of Arr1 with NSF. The interaction between Arr1 and NSF is greater in dark-adapted (DA) retinas compared to light-adapted (LA) retinas (Fig.1d and Fig. 1e). We also observed that NSF was not co-immunoprecipitated with anti-rabbit cone arrestin Pab mCAR-LUMIj in WT retinal homogenates (See Supplemental Fig.2).

Fig. 1
NSF co-immunoprecipitated with Arr1 in mouse retina

To be a relevant interacting partner for Arr1, NSF must co-localize with Arr1 in the appropriate subcellular compartments within the retina. In this study, retinas from WT mice were either light- or dark-adapted; their retinas were prepared for immunohistochemistry and sections were examined with a confocal microscope. The retinal sections were incubated with Arr1 MAb and NSF PAb that were used in the co-immunoprecipitation experiments, followed with the appropriate secondary antibodies, Alexa Fluor 568 goat anti-mouse IgG antibody and Alexa Fluor 488 goat anti-rabbit IgG antibody, respectively. In the DA retinas, the majority of immunoreactive Arr1 was localized to the inner segments, perinuclear region with a small fraction in the synaptic terminals (Fig.2a). In the LA retinas, the majority of Arr1 translocated to the outer segments (Fig.2b). NSF showed some perinuclear labeling in the inner nuclear layer and intensive staining in the outer plexiform layer (OPL) and inner plexiform layer (IPL) in retinas from both LA and DA mice. Dual immunohistochemical labels showed that Arr1 and NSF extensively co-localized in the photoreceptor terminals (OPL; Fig.2c). In the DA retinas, Arr1 and NSF co-localized in the junction between the outer segment and inner segment; however, in the LA retinas, Arr1 immunoreactive label was limited and co-localized with NSF only in the OPL (Fig. 2d). These observations further confirm the potential physiological interactions between Arr1 and NSF in vivo. Their intense dual expression pattern at the specialized ribbon synapse of photoreceptors in the dark condition suggests that Arr1 and NSF may be critical partners for modulating neurotransmitter transmission. Alternatively, Arr1 may be acting at the synapse as a co-chaperone for NSF in the SNARE complex to regulate exocytosis.

Fig. 2
Immunohistochemical fluorescent labeling of NSF and Arr1 in the WT mouse retinas

The Arr1-NSF complex formation is enhanced by ATP

Each NSF monomer has three functional domains (Whiteheart et al., 1994;Nagiec et al., 1995): an N-terminal domain (N, amino acid [AA] 1-205) that is required for the binding to α-SNAP and SNARE proteins; and two homologous ATP-binding domains, D1 (AA 206-477), in which the ATP hydrolytic activity is associated with NSF-driven SNARE complex disassembly, and D2 (AA 478-744), which is responsible for hexamerization. In order to define the potential functional domain in NSF that interacts with Arr1, His6-tagged or Glutathione S-transferase (GST)-tagged NSF-truncated segments of varying lengths were constructed and a GST pull-down assay was performed. Our results indicate that only the NSF fragments that included AA 197-250, which is in the junction of the N and D1 domains (Fig. 3(i)), interact with Arr1.

Fig. 3
Functional analysis of the interaction between Arr1 and NSF. (i) Mapping the binding site of Arr1 on NSF

We further mapped the regions of Arr1 that interact with NSF using in vitro GST pull-down assays. GST-Arr1 (AA 1-191), GST-Arr1 (AA 1-370), GST-Arr1 (AA 1-403) and GST control proteins were immobilized on glutathione-agarose beads and incubated with recombinant His6-NSF. His6-NSF bound specifically to each of the GST-Arr1 recombinant proteins but not the GST control (Fig. 3ii). These data demonstrate that the N-terminal domain (AA 1-191) of Arr1 interacts with NSF. Others have shown the association of NSF with other binding partners exhibited an ATP dependence (Banerjee et al., 1996;Nishimune et al., 1998). In the presence of ATP-γ-s/MgCl2 (non-hydrolyzable ATP) or ATP/ethylenediamine tetra-acetic acid (EDTA) (Fig.4), the interaction of each GST-Arr1 recombinant construct and NSF showed enhanced binding compared with the ATP/MgCl2 (hydrolyzable ATP). These results also confirm that the Arr1 binding to NSF is enhanced by ATP.

Fig. 4
Arr1-NSF complex formation is enhanced by ATP

Interactions between Arr1 and NSF were examined in COS7 cells that were transiently co-transfected with plasmids encoding either empty vector, pcDNA4-HisMax-Arr1 and pcDNA3.1-GFP-NSF (AA 1-744), or pcDNA3.1-GFP- NSF (AA 197-250) for 48 hrs. As shown in Fig. 3iii, both GFP-NSF (AA 1-744) (Fig. 3a) and GFP-NSF (AA 197-250) (Fig. 3d) showed a cytoplasmic diffuse fluorescent pattern. HisMax-Arr1 was localized to the cytosol and perinuclear region. The GFP empty vector (Fig. 3g) showed diffuse staining throughout the cell, including the nucleus. The GFP-NSF (AA 1-744) and GFP-NSF (AA 197-250) extensively co-localized with HisMax-Arr1 (Fig. 3b, 3e, His-Arr1; Fig. 3c, 3f, Merge), whereas the GFP empty vector had no dual staining with HisMax-Arr1 in the COS7 cells (Fig. 3h, HisMax-Arr1; Fig. 3i, Merge).

Arr1 enhances NSF ATPase activity and NSF-driven SNARE complex disassembly

In order to examine the effect of Arr1 binding on NSF ATPase activity, which is critical for NSF function, increasing concentrations of recombinant His6-Arr1 were added to 10μg of recombinant His6-NSF, and the ATPase activity of the NSF was measured by a colorimetric assay. Arr1 significantly enhanced NSF ATPase activity in a dose-dependent manner (Fig.5). We next explored the effect of Arr1 on NSF disassembly activity. NSF has been shown to bind stably to SNARE complex molecules using α-SNAP as an adaptor that locks it in the ATP state (Barnard et al., 1997;Muller et al., 1999). Hydrolysis of ATP enables NSF to separate from and disassemble the SNARE complex. Accordingly, we examined the effect of Arr1 on NSF disassembly of purified, recombinant SNARE molecules. The recombinant His6-NSF proteins were pretreated with increasing concentrations of recombinant Arr1. The GST-tagged-Syntaxin 4 proteins were immobilized to glutathione-agarose beads and then incubated with soluble N-ethylmaleimide sensitive factor attachment protein (α-SNAP) and SNARE polypeptides, including vesicle associated membrane protein 2 (VAMP-2) and SNAP-25 in binding buffer. The mixture of NSF, Arr1 and SNARE complex was precipitated with beads and the precipitated proteins were separated by SDS-PAGE and analyzed on immunoblots with specific antibodies to NSF, Syntaxin-4, SNAP-25 and VAMP2. These data verified that Arr1 enhanced NSF disassembly activity in a dose-dependent manner (Fig. 6b).

Fig. 5
NSF ATPase activity assay
Fig. 6
SNARE complex disassembly assay

Arr1 deletion markedly reduces the expression level of synapse-enriched proteins

Unlike conventional synaptic terminals that release neurotransmitters episodically in response to action potentials, the photoreceptor ribbon synapses are depolarized in the dark, resulting in maintained activation of voltage-gated calcium channels, continual Ca2+ influx, and higher rates of exocytosis required for tonic release of the neurotransmitter glutamate. Increasing light intensity induces a graded hyperpolarization that turns off these events and suppresses glutamate release (Morgans, 2000;von Gersdorff., 2001;Heidelberger et al., 2005). Many presynaptic proteins regulate synaptic vesicle exocytosis and neurotransmitter release. These proteins are differentially distributed among the different synapses of the mouse retina. For example, glutamate transporters, such as vesicular glutamate transporter 1 (vGLUT1), which refills the synaptic vesicles with glutamate, is essential for transmission of visual signaling from photoreceptors to second- and third-order neurons (Johnson et al., 2007). Excitatory amino acid transporter 5 (EAAT5), which is expressed in synaptic terminals of photoreceptors and rod bipolar cells, plays a predominant role in re-uptake of glutamate from the synaptic cleft to ensure reliable synaptic transmission (Wersinger et al., 2006;Pow and Barnett, 2000). Also, a synaptic vesicle protein, VAMP2, which is the predominant isoform in the mouse retina, is an integral membrane protein associated with forming the fusion core complex required for docking and fusing of synaptic vesicles at the synaptic active zone (Sherry et al., 2003). To investigate if the presence of Arr1 alters the expression levels of NSF and the synapse-enriched encoded genes such as vGLUT1, EAAT5 and VAMP2, we performed quantitative RT-PCR to compare the mRNA expression level of these genes in WT and Arr1-/- retinas under different lighting conditions. Comparing DA and LA WT retinas, the mRNA levels of NSF, vGLUT1, EAAT5 and VAMP2 were significantly higher in the dark (Fig.7a). In the light, there was no significant difference in the transcriptional level of NSF and VAMP2 in Arr1-/- retinas compared with WT retinas, but the mRNA expression levels of vGLUT1 and EAAT5 were lower in Arr1-/- retinas than the WT retinas. Difference in expression levels between WT and Arr1-/- retinas is light dependent. In the dark, the mRNA levels of NSF, vGLUT1, VAMP2 and EAAT5 were markedly decreased in the Arr1-/- retinas compared with the WT retinas. The protein expression level of NSF, vGLUT1, VAMP2 and EAAT5 in WT and Arr1-/- retinas under different lighting conditions correlated to the transcription level of these genes (Fig. 7b). For SNAP-25, the protein expression level was lower in the DA Arr1-/- retinas compared with DA WT retinas and there was no significantly difference between LA WT and Arr1-/- retinas.

Fig. 7
Quantitative RT-PCR and protein expression levels of NSF, vGLUT1, VAMP2 and EAAT5 in the retinas

Arr1 deletion suppresses the synaptic activity in the photoreceptor synapse in vivo

To further test if photoreceptor synapses are dysfunctional in vivo in the Arr1-/- retinas, we performed experiments that were based on the widely used fluorescent dye, FM1-43, which can selectively label structures and living cells that are undergoing exocytosis and endocytosis. In WT retinas depolarized with 25mM KCl, FM1-43 was avidly sequestered into the presynaptic terminals in the OPL and IPL (Fig. 8a), whereas in the depolarized Arr1-/- retinas FM1-43 staining in the OPL and IPL was strongly reduced (Fig. 8b). In the non-depolarized WT (Fig. 8c) and Arr1-/- (Fig. 8d) retinas, the FM1-43 uptake was diminished in both OPL and IPL. These results further illustrate that when Arr1 expression is absent in the retina, the synaptic activity is dramatically reduced in the photoreceptors. We also observed FM1-43 staining in the outer segment (Choi et al., 2005) in WT retinas and this staining pattern may indicate the continuous renewal process of the outer segment and dynamic communication of the disc and extracellular space(Chen et al., 2002).

Fig. 8
Synaptic uptake of FM1-43 revealed a decrease of synaptic activity in the Arr1-/- mouse retina

In this study, unlike Arr1, we observed that Arr4 does not interact with NSF (Supplemental Fig. 2), which suggests a divergence of the two visual Arrestins function in the cone photoreceptor. In cones, we have demonstrated in isolated cone photoreceptors that at least one visual Arrestin is essential for normal photoreceptor recovery with increasing light intensity (Nikonov et al, 2008). When the averaged ERG b-wave amplitudes are recorded from the WT, Arr1-/- and Arr4-/- every 2 min from 1 to 15 min during light adaptation (Fig. 9a), the photopic b-wave amplitudes for these three genotypes are similar at 1 min; however, only WT and Arr4-/- mice demonstrated equivalent light adapting increases in the b-wave, reaching maximum amplitudes at 9 min. In contrast, Arr1-/- mice showed no b-wave amplitude increase over the 15 min of light adaptation. Similar results were observed with twenty multiple intensity flashes to further saturate rhodopsin (Brown et al, 2010).

Fig. 9
ERG analysis of WT, Arr1-/-, Arr4-/-, and mCAR-Harr1-/- mice

In order to further delineate the functional divergence between Arr1 and Arr4 in synaptic regulation from their established function in phototransduction inactivation, we also tested mouse cone arrestin (Arr4) transgenic mice (mCAR-Harr1-/-). These Arr1 null mice have normal levels of Arr4 in their cones plus high rod photoreceptor expression levels, which is driven by a rhodopsin promoter to all the rods. Previously, single cell recordings of rods from these mCAR-Harr1-/- mice were observed to restore partial recovery of rod function and morphologically reduced levels of light dependent rod degeneration associated with the loss of Arr1 (Chan et al., 2007). In Fig.9b, the 15 min of light adaptation showed no appreciable increase in amplitudes of b-wave ERG responses in the mCAR-Harr1-/-, similar to the observed phenotype in the Arr1-/-.

Discussion

In this study, we demonstrate the alternative structural and potential functional interactions between visual Arr1 and NSF in the photoreceptor synapse using a combination of biochemical, cellular, and molecular biological techniques.

The crystal structures of Arrestin family members, including Arr1, Arr4 and β-arrestin1, are remarkably similar (Wilden et al., 1997;Hirsch et al., 1999;Han et al., 2001;Sutton et al., 2005). In a previous in vitro yeast two-hybrid study, β-arrestin1 was shown to interact in vitro with NSF and it bound to the identical region (AA 197-250 of NSF) as observed for Arr1 binding. The β-arrestin1-NSF complex formation was also ATP-dependent (McDonald et al., 1999). Over expression of NSF rescued the dominant negative effect of a β-arrestin1 phosphorylation mutant (S412D) by restoring normal sequestration of the GPCR, the β2-adrenergic receptor (AR). It is important to note that other members of the Arrestin family do not possess the Ser412 residue and therefore are not subject to the same regulation in receptor internalization as β-arrestin1. In our work, we show that the Arr1 N-terminal motif (AA 1-191) contains a key domain involved in activation-recognition and phosphorylation-specific binding of the Arr1 (Gurevich et al., 1994;Gurevich and Benovic, 1993;Gurevich and Benovic, 1992) for interaction with NSF, but no evidence documents that this region of β-arrestin1 interacts with NSF. We also determined that cone Arr4, which is also expressed in cone pedicles with Arr1 (Nikonov et al., 2008), did not interact with NSF (Supplemental Fig.2). In the previous studies, the interaction of β-arrestin1 with NSF directs the clathrin-mediated receptor internalization. In our experiments, Arr1 binds directly to the junction of the NSF N and D1 domains, which are required for SNARE complex binding and ATP hydrolysis, to modulate the process of exocytosis.

Unlike conventional synaptic terminals that release neurotransmitter episodically in response to action potentials, the photoreceptor ribbon synapses, which are specialized structures at active zones in the rod spherules and cone pedicles, are depolarized in the dark, resulting in maintained activation of voltage-gated calcium channels, continual Ca2+ influx, and higher rates of exocytosis required for tonic neurotransmitter (L-glutamate) release (Morgans, 2000; von Gersdorff, 2001; Heidelberger et al., 2005). Increasing light intensity induces a graded hyperpolarization that turns off these events and suppresses neurotransmitter release. This higher rate of exocytosis in the photoreceptor synapses must be balanced by compensatory endocytosis to retrieve vesicle membrane and vesicle proteins incorporated into the plasma membrane during fusion.

Our study demonstrates that Arr1 interacts with NSF to enhance its ATPase activity and to also stimulate its ability to disassemble the SNARE complex. These functions are crucial for NSF in regulation of vesicular transport and synaptic transmission. Moreover, Arr1 deletion markedly reduces the expression level of NSF and synapse-enriched proteins, including vGLUT1, EAAT5, VAMP2 and SNAP-25. SNAP-25 and VAMP2 are the major components for SNARE complex formation (Sogaard et al., 1994;Roth and Burgoyne, 1994). The vGLUT1 regulates the glutamate sequestered into synaptic vesicles in photoreceptor terminals (Johnson et al., 2007), and EAAT5 is involved in synaptic uptake of glutamate into photoreceptor to ensure reliable synaptic transmission (Wersinger et al., 2006). Dynamic exocytosis, vesicle replenishment and glutamate removal are principally responsible for regulating the kinetics of synaptic transmission in the photoreceptors (Thoreson, 2007).

We observe that a loss of Arr1 gene expression in the retina of Arr1-/- mice is also associated with a reduced level of mRNAs and proteins involved in these processes, and synaptic activities are dramatically suppressed in the photoreceptors of these mice. On the basis of previous studies (Mendez et al., 2003;Smith et al., 2006;Brown et al., 2010) and our current findings, we suggest that Arr1 serves a dual functional role in distinct subcellular compartments in the photoreceptor. Bright light exposure induces Arr1 translocation to the photoreceptor outer segment for termination and recovery of the light response by binding to light-activated, G-protein receptor kinase1 dependent phosphorylated rhodopsin (Wilden et al., 1986; Xu et al., 1997). In the dark, Arr1 is highly expressed and maintained in all mouse photoreceptor synapses where it interacts with NSF for modulation of sustained synaptic vesicles release to fulfill the need to accurately encode subtle membrane potential changes leading to specific adaptations of the synaptic machinery employed in the photoreceptor synapses.

Mutations in the ARR1 gene lead to Oguchi disease (Fuchs et al., 1995; Nakazawa et al., 1997) and retinitis pigmentosa (Nakamachi et al., 1998). Oguchi disease is a rare recessive form of congenital stationary night blindness (CSNB) characterized by distinctive golden-brown discoloration of the fundus that disappears after prolonged dark adaptation. The typical ERG findings in patients with Oguchi disease show a negative configuration with subnormal to normal a-waves and nearly absent b-waves, similar to those in complete CSNB. After longer dark adaptation (2-4 hrs), the mixed rod-cone ERG shows increases in both a- and b-waves. This recovery of light sensitivity was thought to be related to the time course of rhodopsin regeneration (Dryja, 2000). From another perspective, our study suggests an alternative explanation with Arr1 involvement. The b-wave reflects the depolarizing response of bipolar cells and the absence of a b-wave suggests that the synaptic transmission between photoreceptors and depolarizing bipolar cells may be defective. In our experiments, we observe that Arr1 enhances NSF ATPase activity and also facilitates SNARE complex disassembly to maintain tonic neurotransmitter release.

We also observe an abnormal photopic b-wave phenotype associated with loss of light adaptation in the Arr1-/- mice (Fig.9a and [Brown et al., 2010]). Increased light adaptation gradually changes the synaptic signal transfer from photoreceptor synapse to the second order neuron. We propose that this phenotype reflects a defect of intercellular feedback mechanisms dependent on synaptic transmission downstream of the light-activated, phosphorylated GPCR recovery observed with Arr1 function (Van Epps et al., 2001). Mouse rod photoreceptors only express Arr1, while the cone photoreceptors express both Arr1 and Arr4. When recording electrophysiological signals from single mice cones with either one or both visual Arrestins knocked out, at least one visual Arrestin is required for normal cone inactivation (Nikonov et al., 2008). In other studies, Arr4 was shown to partially substitute for Arr1 in Arr1-/- rod photoreceptors in transgenic mCAR-Harr1-/- retina (Chan et al., 2007), which targeted the expression of Arr4 to rod photoreceptors on an Arr1 null background. In Fig.9b, we showed the light-adaptation b-wave curve in mCAR-Harr1-/- retina is almost identical to the Arr1-/-. This suggests that even though Arr4 can partially substitute for the function of Arr1 in rod phototransduction inactivation, it cannot substitute for the function of Arr1 in photoreceptor synaptic regulation since Arr4 does not interact with NSF (Supplemental Fig.2). Therefore, the ERG b-wave abnormality in patients with Oguchi disease, who lack normal expression of ARR1 may result from the disruption in synaptic transmission in the photoreceptor terminals. Photoreceptors can properly respond to the change in light stimuli depending on precise control of neurotransmitter release. Reliable photoreceptor synaptic transmission requires accurate detection of the changes in glutamate concentration in the synaptic cleft by the postsynaptic cells. We propose that without Arr1 acting as a modulator for NSF, the photoreceptor synapse cannot maintain the normal graded exocytosis rate to continuously adjust the release of glutamate to optimize the signal transfer to the postsynaptic horizontal and bipolar cells.

In summary, we have demonstrated that the interaction of Arr1 and NSF is ATP-dependent, and the N-terminal domain of Arr1 interacts with the N and D1 functional domains of NSF. The Arr1-NSF interactions are greater in the photoreceptor synaptic terminal in the dark. Furthermore, Arr1 enhances the NSF ATPase activity and increases the NSF disassembly activities, which are critical for NSF functions in sustaining a higher rate of exocytosis in the photoreceptor synapses and the compensatory endocytosis to retrieve vesicle membrane and vesicle proteins for vesicle recycling. Deletion of Arr1 expression also leads to reduced levels of mRNA and protein expression of NSF, vGLUT1, EAAT5 and VAMP2, which have the potential to markedly depress the exocytosis rate in the dark-adapted retinas. These data provide strong in vitro results and for the first time, in vivo evidence that, in photoreceptors demonstrate the Arr1 and NSF interaction is necessary for the maintenance of normal vision. This study also reveals a novel functional role of Arr1 in photoreceptor synapses and provides further insights into potential mechanisms of inherited retinal diseases, such as Oguchi disease and ARR1-associated retinitis pigmentosa.

Supplementary Material

Supp1

Acknowledgments

CMC is the Mary D. Allen Chair in Vision Research, DEI, and a Research to Prevent Blindness (RPB) Senior Scientific Investigator. This work was supported, in part, by NIH Grant EY015851 (CMC), EY03040 (DEI), RPB (DEI & CMC), Dorie Miller, Tony Gray Foundation, Mary D. Allen Foundation (Dr. Richard Newton Lolley Memorial Scholarship [SPH]). We thank members of the Mary D. Allen Laboratory for scientific discussions and critical reading of the manuscript, Lawrence Rife for his technical expertise with ERG analysis, Drs. Larry A. Donoso for Mab D9F2, Jeannie Chen for providing Arr1-/- and mCAR-Harr1-/- mice, David Pow for anti-EAAT5 polyclonal antibody and C.-P. Ko for expert technical advice in FM1-43 experiments.

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