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J Biomol Tech. 2010 September; 21(3 Suppl): S69.
PMCID: PMC2918225

The Mayo Clinic Advanced Genomic Technology Center Microarray Shared Resource

J. Jen
Mayo Clinic, Rochester, MN, United States

Abstract

CF-7

The Mayo Clinic Advanced Genomic Technology Center Microarray Shared Resource provides a full range of services from RNA quality assessment to whole genome transcript measurement for researchers both inside and outside of the Mayo Health System. Created in 2000, the facility offers technical services and support for both basic and clinical research programs for more than 80 principle investigators annually. Specializing in high-density microarray and real-time PCR-based analyses, the lab provides instrumentation and technical expertise to perform genomic studies on a wide variety of different sample types, including, but not limited to, RNA samples derived from fresh frozen tissues, formalin-fixed paraffin-embedded tissues, Laser Capture Microdissected cells, and cultured cell lines. Instrument platforms present in the Microarray Shared Resource include Affymetrix GeneChip™;, Illumina BeadChip™, Applied Biosystems 7900 HT Sequence Detection Systems, Fluidigm Biomark™ and Agilent 2100 Bioanalyzers. Affymetrix GeneChip™ analyses are performed routinely on all human, mouse, and rat whole transcriptome arrays, as well as Gene 1.0, Exon, Tiling, and miRNA GeneChip™ products. In addition, Illumina BeadChip™ analyses are conducted with human and mouse Whole Genome 6, HT-12, Ref8, and Whole Genome DASL assays. As genomic technologies continue to emerge and evolve, the Microarray Shared Resource also seeks to expand its capabilities to include gene expression profiling in single cells as well as mRNA and miRNA analyses using Fluidigm high throughput chips and Next Generation sequencing technologies.


Articles from Journal of Biomolecular Techniques : JBT are provided here courtesy of The Association of Biomolecular Resource Facilities