PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jbtJBT IndexAssociation Homepage
 
J Biomol Tech. 2010 September; 21(3 Suppl): S44.
PMCID: PMC2918223

Electrophoretic Nucleic Acid Concentration with Application to Integrated Sequence Enrichment and Detection

J. Thompson,2 J. Pel,1 G. Shibahara,1 and A. Marziali1,2
1Boreal Genomics Inc., Vancouver, BC, Canada;
2Department of Physics and Astronomy, University of British Columbia, Vancouver, BC, Canada

Abstract

RP-74

We have developed a novel electrophoretic concentration technology, named SCODA (Synchronous Coefficient of Drag Alteration) for efficiently purifying and concentrating nucleic acids.SCODA excels in applications where common extraction techniques can fail by offering unique advantages including exceptional contaminant rejection and unparalleled yield in low abundance nucleic acid samples. Due to the flexibility of the SCODA system, we are able to process samples in a variety of complex matrices, including samples that contain particulates or strong PCR inhibitors. We are also able to recover nucleic acids from extremely dilute samples, with successful concentration from starting DNA concentrations in the zeptomolar range. The non-mechanical nature of the process has allowed recovery of intact high molecular weight DNA over 1Mb.More recently we have demonstrated that SCODA can be made specific to the sequence of DNA targets to be concentrated, offering the opportunity for integrated sequence enrichment and detection applications. This presentation will give a brief overview of the SCODA technology with emphasis on recent progress in sequence specific DNA concentration.


Articles from Journal of Biomolecular Techniques : JBT are provided here courtesy of The Association of Biomolecular Resource Facilities