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J Biomol Tech. 2010 September; 21(3 Suppl): S26.
PMCID: PMC2918175

Implementing Quality Control in the Next-Generation Sequencing Workflow

S. Glück, M. Gassmann, and R. Salowsky
Agilent Technologies, Waldbronn, Germany

Abstract

RP-20

Next-generation sequencing (NGS) has revolutionized the genetic landscape. It is a lengthy, labor-intensive process that yields results never before achieved. As a result, it is imperative that the quality of the RNA or DNA starting material as well as DNA libraries be evaluated from the start. Difficulties can often arise when only fragmented or low concentrated starting material is available. Furthermore, most NGS sample preparation protocols require PCR amplification to generate DNA libraries prior to sequencing, the likelihood of artifact generation could contribute to bias, affecting the potential results. Here we discuss several critical steps in the next-generation sequencing workflow which require the implementation of quality control procedures to increase workflow efficiency, which in turn helps to reduce downstream sequencing costs.


Articles from Journal of Biomolecular Techniques : JBT are provided here courtesy of The Association of Biomolecular Resource Facilities