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J Biomol Tech. 2010 September; 21(3 Suppl): S59.
PMCID: PMC2918164

Proteomic Analysis of the Helicobacter pylori ArsRS Regulon by DIGE-MS

J. Loh, S. Gupta, A. Krezel, and T. Cover
Vanderbilt University, Nashville, TN, United States

Abstract

RP-119

The ArsRS two-component signal transduction system contributes to acid-responsive gene expression in H. pylori. We used DIGE-MS to examine quantitative changes in protein expression within the proteomes of a wt vs. arsS mutant strain grown under acidic (pH 5) and neutral (pH 7) conditions (N=4). Multivariate statistical analysis on the protein features matched across the DIGE dataset demonstrated that the majority of the variance enabled the differentiation of the two strains, with a smaller subset of pH-responsive expression changes. ArsR gel mobility-shift assays (EMSA) were used to confirm previously-known proteins under the aegis of this control, as well as highlight novel components.


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