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J Biomol Tech. 2010 September; 21(3 Suppl): S75.
PMCID: PMC2918152

Trudeau Institute Molecular Biology Core Facility

A. E. Moquin and J.J. Hoffman
Trudeau Institute, Saranac Lake, NY, United States

Abstract

CF-25

Centralization and merging of core facilities is a favorite topic in the current economy. The Molecular Biology Core Facility (MBCF) at the Trudeau Institute has provided a variety of services to the investigators at the institute while recovering the majority of its expenses since its inception 12 years ago. The MBCF survives by offering an assortment of services that are specifically tailored to the Institute mission and by personalizing each service to the individual investigator. A five-year core grant, as part of a program project, helped to cover the cost of developing new techniques. This grant has expired and we are currently actively seeking a way to replace those funds. Services provided include DNA sequencing, spectratyping of the T-cell repertoire (fragment analysis), production, purification and labeling of Major Histocompatibility (MHC) Class I and Class II multimers for Fluorescent Activated Cell Sorter (FACS) analysis, real-time PCR measurement of gene expression and viral loads, knock-out mouse and Mycoplasma screening, DNA haptenation and recombinant protein expression /purification for antibody detection and in vivo immunization. A variety of simple services such as primer design, primer ordering, stock primers, peptide ordering and Taq production save the investigators time, effort and money. Education and training are provided for all techniques and for using MBCF instrumentation, such as spectrophotometers, tissue processors, real-time PCR equipment and image capture/analysis equipment. Not only does the MBCF provide the service, the personnel assist in the planning and analysis to maximize proper usage of the technique. The MBCF at Trudeau Institute survives through versatility, imagination and customization


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