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The Florida Center of Excellence for Biomolecular Identification and Targeted Therapeutics (FCoE-BITT, www.bitt.usf.edu, located on the Tampa campus of the University of South Florida (USF), is a comprehensive center that enhances interactions between scientists and engineers to identify molecules of human health significance and to develop novel methods for use in diagnosis, prevention and treatment of human disease. FCoE-BITT encompasses the full range of product development, from discovery to commercialization. It is expected to create an infrastructure that supports collaboration across several academic units (USF Health, USF College of Arts and Sciences, USF College of Engineering) and move research into commercial applications through its close collaboration with various technology transfer resources. In support of this mission, the FCoE-BITT recently opened three fee-for-service core laboratories: the Proteomics Core, the NMR Core and the Biotechnology Development and Testing Facility. The Proteomics Core supports large-scale protein isolation, identification and characterization (mass spectrometry, differential expression profiling, analysis of post-translational modifications of proteins, quantitative proteomic analyses). The NMR Core utilizes two Varian NMR systems (600 and 800 MHz) for protein structure biology and dynamics determinations, and for structural analyses of natural and synthetic organic molecules for pharmaceutical development. The Biotechnology Development and Testing Facility provides equipment for material fabrication/analysis and for performing in vitro tests on biological samples, four suites for eukaryotic and bacterial cell culture (to biosafety level 2) as well as custom testing services, consultation and training for drug discovery, drug delivery and medical device/sensor development projects. These three FCoE-BITT core laboratories serve USF faculty and students, external academic institutes and regional companies.