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The Vermont Genetics Network (VGN) Proteomics Facility supports biological and biomedical research primarily throughout Vermont, but also welcomes sample submission from investigators outside of Vermont. The facility uses state-of-the-art mass spectrometry for analyzing proteins and peptides. Facility personnel acquire and analyze proteomic data and conduct training in proteomic methods and experimental design. Pre-submission and post-data acquisition consultations are arranged with facility directors. Details can be found at www.uvm.edu/~vgn/proteomics. The facility is led by Co-Directors Dr. Bryan Ballif and Dr. Dwight Matthews. Facility manager, Dr. Bin Deng interacts with investigators and runs proteomics analyses. Dr. Jim Vincent, VGN Bioinformatics Core director, provides bioinformatics support and Dr. Janet Murray coordinates the outreach to Vermont Baccalaureate Partner Institutions. The Proteomics Facility has three mass spectrometers each coupled to nanoflow high performance liquid chromatography (nano-HPLC). The mass spectrometers are two Thermo-Finnigan LTQ linear quadrupole ion trap mass spectrometers and one Thermo-Finnigan LTQ-Orbitrap hybrid mass spectrometer. Since last year over 2000 samples have been analyzed, twelve peer-reviewed papers have been published and 17 grant proposals have been supported by the proteomics facility. The user base for the proteomics facility is growing and includes over 34 faculty/post doctorate/staff, 28 graduate students and 7 undergraduates. At least 11 seminars and 11 poster presentations have been presented by users/staff that have presented VGN proteomic data. It is an exciting time for proteomics in Vermont. The facility is funded by the VGN through NIH grant P20 RR16462 from the INBRE Program of the National Center for Research Resources.