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A “living microarray” technology has been developed to enable high throughput screening and analysis of bacterial and mammalian cells. This novel approach augments traditional microarray technology which has been extensively used to study proteins and nucleic acids. Our living microarray utilizes micro-scale “quill-pen” cantilevers to transfer living cells onto a wide variety of surfaces. In contrast with existing cell deposition methods, such as ink jet or laser ablation, the quill-pen approach imparts minimal thermal and shear stress to cells, preserving cell viability and biological functionality. Cell viability and proliferation has been evaluated, demonstrating the feasibility of this printing method and its compatibility with a wide range of cell types. These living arrays have applications for cell-based screening assays, tissue engineering, cell signaling studies, and for directly interfacing cells with nanodevices and biosensors.