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J Biomol Tech. 2010 September; 21(3 Suppl): S13.
PMCID: PMC2918106

What to do when Protein Expression Goes Wrong

Abstract

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Expression of proteins in bacteria is a very common practice, and has met with many outstanding successes.However, usually only around 50 or 60 percent of genes will express a suitable product in bacteria that can be purified in a soluble and active form.The purpose of this session is to present some of the solutions that have been commonly found to work for bacterial expression systems to increase the success rates. Further, many laboratories are using alternate systems for protein production, the most common of which is probably baculovirus-based insect cell systems. The pros and cons of offering such a system in a core resource will be addressed. Finally, we hope to have an open discussion where successful (and not so successful) rescue strategies for protein expression can be discussed.


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