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J Biomol Tech. 2010 September; 21(3 Suppl): S13.
PMCID: PMC2918051

Baculovirus Expression: Over Expression or Over Expectation

Abstract

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The baculovirus system has become a preferred form of expression for researchers working with kinases and other eukaryotic proteins. Such proteins are generally assumed to express better in an insect cell-based system rather than a bacterial one. As a core facility offering protein expression and purification, are we really able to over express proteins in a baculovirus-based system or this an over expectation? During the past decade, there has been an enormous increase in the availability of vectors and reagents for use in baculovirus-based protein expression in insect cells.This increase has been paralleled and, to certain extent, fuelled by the great demand to study the structures and functions of proteins for therapy and diagnosis of various diseases. This presentation discusses some of the pros and cons of using a baculovirus based system with specific examples from our service, using two different vectors.Based on our experience, the baculovirus system certainly delivers overexpression of proteins in insect cells. In fact, many of our investigators skip bacterial expression altogether and go straight for baculovirus systems.


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