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J Biomol Tech. 2010 September; 21(3 Suppl): S16–S17.
PMCID: PMC2917997

Joint Ressearch Group Presentation on Proteomics (PRG, sPRG and iPRG)

C. Turck4
1Vanderbilt University, Nashville, TN, United States;
2National Institute of Standards and Technology, Gaithersberg, MD, United States;
3University of Virginia, Charlottesville, VA, United States;
4Max Planck Institute of Psychiatry, Munich, Germany

Abstract

r4-1

Three presentations will be given to highlight the 2010 Research Group studies in proteomics (PRG), proteomics informatics (iPRG) and proteomics standards (sPRG).This year's PRG study was based on a standard protein identification project from a defined collection of biologically-related human proteins, and is modeled after a real-life scenario.Additional challenges of 15N-labeled proteins (one of which was incorrectly purified as an E. coli contaminant) along with an altered recombinant protein form made the study amenable to a wide spectrum of expertise levels.The iPRG and sPRG studies both focused on phosphoproteins. The sPRG designed a study that would be suitable for use in assessing a laboratory's capabilities for detecting singly and multiply phosphorylated peptides against a background digest of the same proteins.Enough sample was sent to requesters to allow for multiple analyses, and results were reported back using an online survey and were analyzed to assess approaches to phosphopeptide detection.The iPRG provided study participants with an LC-MS/MS dataset from a lysate digested with trypsin and enriched for phosphopeptides using strong cation exchange fractionation followed by immobilized metal affinity chromatography (SCX/IMAC). The iPRG study was designed to enable participants to evaluate their data analysis capabilities for both identifying and localizing sites of phoshorylation.


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