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Tropical Medicine & International Health
Trop Med Int Health. 2010 July; 15(7): 776–795.
PMCID: PMC2916221

Impact of hookworm infection and deworming on anaemia in non-pregnant populations: a systematic review



To summarise age- and intensity-stratified associations between human hookworm infection and anaemia and to quantify the impact of treatment with the benzimidazoles, albendazole and mebendazole, on haemoglobin and anaemia in non-pregnant populations.


Electronic databases (MEDLINE, EMBASE, PubMed) were searched for relevant studies published between 1980 and 2009, regardless of language, and researchers contacted about potential data. Haemoglobin concentration (Hb) was compared between uninfected individuals and individuals harbouring hookworm infections of different intensities, expressed as standardised mean differences (SMD) and 95% confidence intervals (CI). Meta-analysis of randomised control trials (RCTs) investigated the impact of treatment on Hb and anaemia.


Twenty-three cross-sectional studies, six pre- and post-intervention studies and 14 trials were included. Among cross-sectional studies, moderate- and heavy-intensity hookworm infections were associated with lower Hb in school-aged children, while all levels of infection intensity were associated with lower Hb in adults. Among RCTs using albendazole, impact of treatment corresponded to a 1.89 g/l increase (95%CI: 0.13–3.63) in mean Hb while mebendazole had no impact. There was a positive impact of 2.37 g/l (95%CI: 1.33–3.50) on mean Hb when albendazole was co-administered with praziquantel, but no apparent additional benefit of treatment with benzimidazoles combined with iron supplementation. The mean impact of treatment with benzimidazoles alone on moderate anaemia was small (relative risk (RR) 0.87) with a larger effect when combined with praziquantel (RR 0.61).


Anaemia is most strongly associated with moderate and heavy hookworm infection. The impact of anthelmintic treatment is greatest when albendazole is co-administered with praziquantel.

Keywords: hookworm, Necator americanus, Ancylostoma duodenale, anaemia, haemoglobin, anthelmintic treatment


Hookworms (Necator americanus and Ancylostoma duodenale) reside in the small intestine of infected individuals where they attach themselves to the villi and feed on host blood. Among individuals with inadequate iron intake and high physiological demands, this blood loss can result in anaemia. The link between hookworm and anaemia was first established in the nineteenth century (Perroncito 1880), and during the subsequent 130 years, there have been numerous reviews of the extensive literature in this area (Layrisse & Roche 1964; Miller 1979; Schad & Banwell 1984; Crompton & Stephenson 1990; Crompton & Whitehead 1993; Stoltzfus et al. 1997; Brooker et al. 2004; Hotez et al. 2004). There is a direct relationship between the number of hookworms an individual harbours (the intensity of infection) and the amount of intestinal blood lost attributable to hookworm (Gilles & Williams 1964; Martinez-Torres et al. 1967; Stoltzfus et al. 1996). The clinical consequences of this loss will depend on the host’s underlying iron status as well as the presence of other causes of anaemia (Fleming 2000). Studies indicate that there is some worm burden threshold above which clinically significant anaemia is likely to occur, with the precise threshold dependent on the host’s iron status (Lwambo et al. 1992). As well as influencing morbidity, worm burden is a key determinant of transmission dynamics and hence the rate of reinfection following anthelmintic treatment (Anderson & May 1985). Intensity of infection may also influence the efficacy of treatment (Bennett & Guyatt 2000). It follows therefore that as the intensity of hookworm infection varies considerably between populations, the risk of anaemia attributable to hookworm and the impact of treatment will differ among populations.

In 2007, a systematic review of randomised controlled trials (RCTs) investigating the impact of anthelmintic treatment reported an increase in haemoglobin concentration (Hb) of 1.71 g/l after treatment (Gulani et al. 2007). But this review did not distinguish between different helminth species or account for intensity of infection, which may have underestimated the true treatment effect (Awasthi & Bundy 2007); the effect of treatment is likely to be greatest where hookworm is most prevalent and intense. Recent work has quantified hookworm-related anaemia among pregnant women (Brooker et al. 2008). The present work aims to quantify the impact of hookworm infection and anthelmintic treatment using benzimidazoles, albendazole and mebendazole, among non-pregnant populations in hookworm-endemic areas. Specifically, we review available data from cross-sectional studies that investigated the relationship between intensity of hookworm infection and Hb. We also summarise available data from RCTs and pre- and post-intervention observational studies that compared the effects of benzimidazole treatment, either alone or in combination with the anti-schistosomal drug praziquantel, on Hb and anaemia levels. Finally, based on the value of combining deworming with micronutrient supplementation in children, we evaluate the impact of treatment in combination with iron supplementation (Hall 2007).This work contributes to the current reassessment of the global burden of disease (Murray et al. 2007).


Identification of cross-sectional studies

The bibliographic databases of MEDLINE (, EMBASE ( and PubMed ( were searched for relevant studies in 2006 and again in April 2009. For analysis of the association between intensity of hookworm infection and anaemia, the following Medical Subject Headings (MSHs) were used to identify relevant studies published between 1980 and 2009: hookworm, Necator americanus, Ancylostoma duodenale, an(a)emia, h(a)emoglobin and h(a)ematocrit. Cross-sectional studies published prior to 1980 were reviewed, but those presenting relevant statistics were found to use different diagnostic test and intensity thresholds, making comparisons with later studies difficult (Beaver 1951; Carr 1926; Chernin 1954). Returned abstracts were reviewed and full texts retrieved if they contained relevant information. References from articles and key reviews were screened for additional studies. Finally, leading researchers in the area and authors of key papers were contacted to ask about unpublished or unindexed data, and this yielded a number of additional studies. Non-English language journals were included in the search, and relevant articles were assessed against the inclusion/exclusion criteria by native speakers. No distinction could be made between the two different hookworm species, Necator americanus and Ancylostoma duodenale, as none of the studies used diagnostic methods able to differentiate species.

The primary outcome for analysis was haemoglobin concentration (Hb) in non-pregnant populations, and our hypothesis was that haemoglobin concentration is associated with the intensity of hookworm infection as assessed by quantitative egg counts, expressed as eggs per gram (epg)/faeces. Abstracted data included the mean Hb, corresponding standard deviation (SD) and number of individuals infected for each category of hookworm infection intensity and were entered into an Excel database. When data were not reported in the preferred format, authors were contacted to request relevant data summaries. Data were stratified by age group (0–4, 5–19 and 20+ years) and category of infection intensity (light, 0–1999 epg; moderate, 2000–3999 epg; heavy, 4000+ epg)) (WHO 2002).

Identification of treatment studies

Treatment studies were identified using the MSHs deworming, anti-helmint(h)ic, anthelmint(h)ic, anthelminth, mebendazole, praziquantel, pyrantel, piperazine, nitazoxanide, levamisole, albendazole, bephenium and niclosamide. Only trials that randomised individuals to treatment with a benzimidazole (BMZ) anthelmintic drug and a control group, either placebo or standard of care, and conducted in hookworm-endemic areas were included. Only studies from 1980 onwards were identified because mebendazole was only introduced to the market in 1975 and albendazole in 1980 and use of these benzimidazoles in public health interventions post-dates 1980 (Horton 2003). Two additional groups of studies were included: (i) RCTs of BMZ combined with praziquantel (PQZ) treatment for schistosomiasis and (ii) RCTs of BMZ treatment combined with iron supplementation. In addition to RCTs, observational studies of the impact of intervention were reviewed. Studies that did not quantify the baseline prevalence of hookworm infection, were conducted in pregnant populations, or used an anthelmintic other than albendazole (ABZ) or mebendazole (MBZ) were excluded as these drugs are not widely used in large-scale treatment programmes. Trials were assessed by recommended criteria as shown in Table S2, but quality was not summarised using a score and incomplete reporting was not followed up with authors. These decisions were based on reported unreliability of scales in assessing quality and on the possibility of introducing bias (Higgins & Green 2009).

Primary outcomes were change in mean Hb and prevalence of anaemia, based on the hypothesis that Hb will differ between intervention and control group in response to anthelmintic treatment. Abstracted data included the baseline prevalence of hookworm infection and anaemia, and the post-treatment relative risk of hookworm infection, prevalence of anaemia, mean Hb and change in Hb in each group, with corresponding SDs. For studies that did not report the prevalence of anaemia, an approximation was made on the basis of the reported mean and standard deviation Hb. The proportion of individuals with Hb below the age-specific thresholds for mild, moderate and severe anaemia was calculated assuming a normal Hb distribution (Sharman 2000; WHO 2008). For studies that evaluated treatment effect at multiple time points, only data from the longest time interval were included in the analysis.

Data analysis

The difference in Hb among different intensity categories was expressed as pool estimates of standardised mean difference (SMD) based on a meta-analysis using a DerSimonian and Laird random effects model. All P values are from two-tailed tests of significance where alpha is equal to 0.05.

The impact of treatment was assessed using two approaches. First, for RCTs and observational studies, the impact of treatment on the prevalence of anaemia was expressed as a relative risk (RR) and mean impact summarised. Second, for RCTs, a DerSimonian and Laird random effects meta-analysis was conducted to provide pooled estimates of the effect of treatment on Hb, and a metaregression was used to identify sources of variation between studies. The analysis was stratified by co-administration of PQZ in the intervention arm. This design was justified by the lack of an equivalent intervention in the control arm of these trials and their incomparability to those specifically evaluating the impact of BMZ treatment. SMDs were transformed into g/l using a mean of the SDs of included studies. Linear regression analysis was used to summarise relative risk of mild and moderate anaemia and identify potential determinants of anaemia impact. Study characteristics that were investigated in the modelling process included: age category, WHO region, intervention, baseline prevalence and intensity of hookworm infection, mean Hb at baseline, dosage schedule and follow-up period. Sensitivity analysis identified the Stephenson et al. (1993) study as responsible for significant variation in the results, and this study was therefore excluded from further analysis on the basis that participants were restricted to those with heavier infections.

Heterogeneity between studies was assessed by an I2 test, with values greater than 50% representing significant heterogeneity, and a sensitivity analysis and preliminary metaregression identified potential sources of variation. Results were displayed as forest plots. Publication bias was investigated by the construction of funnel plots and by the Egger and Begg statistical tests. Analysis was performed using the ‘metan’ and related functions in STATA version 10 (College Station, TX).


Associations between hookworm intensity and haemoglobin

The search identified 423 citations, from which 117 unique and potentially relevant articles were retrieved. Of these, 48 were determined to be eligible and 14 had suitable cross-sectional data, including 11 surveys among school-aged children and seven among non-pregnant adults1. In addition, unpublished data were available for nine studies. Eighteen studies were conducted in Africa, one in South Asia, four in Southeast Asia and one in Latin America. Survey characteristics are described in Table S1. For all populations, prevalence estimates for hookworm infection ranged from 0.3 to 96%, with 12.5% of the surveys having a mean intensity of infection >1000 epg and eight studies having no individuals with infection intensity >2000 epg. Prevalence of anaemia (110 g/l or 120 g/l threshold) ranged between 4.5 and 90%.

Figure 1 presents the difference in Hb between school-aged children uninfected and those harbouring different levels of infection intensity. There was no evidence for a difference in Hb between uninfected and lightly infected children (SMD −0.04, 95%Confidence interval [CI]: −0.11 to 0.03) (Figure 1a), but there was evidence for a difference between uninfected children and moderately (SMD −0.32, 95% CI −0.46 to −0.18) or heavily (SMD −0.64, 95% CI −0.84 to −0.45) infected children (Figure 1b). There was significant heterogeneity in differences between studies but that could not be explained by any single study. However, a higher baseline prevalence of anaemia was weakly associated with lower Hb in lightly or moderately infected children compared to uninfected children (P= 0.06), suggesting that children with poor underlying iron status may be more likely to suffer the consequences of light hookworm infection than those with better nutritional status. There was some evidence of non-symmetry in the funnel plot of uninfected children compared to those with a moderate infection and weak evidence of publication bias using the Egger’s test but not Begg’s test.

Figure 1
Forest plot of the difference in haemoglobin concentration (Hb) among school-aged children (a) uninfected with hookworm and children with a light (1–1999 eggs/gram) hookworm infection and (b) uninfected with hookworm and children with a heavy ...

Among adults, there was evidence for progressively lower Hb among individuals lightly infected (SMD −0.15, 95% CI −0.29 to −0.00) (Figure 2a), moderately infected (SMD −0.47, 95% CI −0.77 to -.17) and heavily infected (SMD −0.93, 95% CI −1.43 to −0.44) relative to those uninfected (Figure 2b). There was evidence of heterogeneity of effect that could be explained by specific studies in each infection strata which, when excluded, altered the SMD (in parenthesis): Brooker et al. (2007a), the only study in Latin America (SMD −0.21, 95% CI −0.30 to −0.11); Latham et al. (1982) among Kenya male road workers in Kenya (SMD −0.36, 95% CI −0.53 to −0.19); and Olsen et al. (1998) in a highly malaria endemic area (SMD −0.71, 95% CI −1.07 to 0.34). No evidence of publication bias was detected in any of the other age group or intensity strata.

Figure 2
Forest plot of the difference in haemoglobin concentration (Hb) among non-pregnant adults (a) uninfected with hookworm and adults with a light (1–1999 eggs/gram) hookworm infection and (b) uninfected with hookworm and adults with a heavy (4000+ ...

Impact of anthelmintic treatment

Of the 31 studies identified, 14 RCTs met the criteria for inclusion, of which 10 evaluated the effects of either ABZ or MBZ treatment alone (Table 1), four evaluated ABZ treatment with PQZ (Table 2), and five evaluated treatment in combination with iron supplementation (Table 3). In addition, six observational studies were included (Tables 1–3). The majority of studies were conducted in Africa (75%), predominantly in East Africa, used ABZ (85%), and were conducted among school-aged children (75%) (Table 4). Of the RCTs, most studies were individually randomised (79%) and double blind (77%), while seven used a factorial study design to evaluate deworming in combination with iron supplementation. The mean follow-up period for all studies was 3.8 months. Marked variation in the prevalence of hookworm and anaemia (using different thresholds) existed between studies.

Table 4
Summary of 20 hookworm intervention studies investigating the impact of benzimidazole treatment (BMZ) on haemoglobin concentration (Hb), administered alone or in combination with praziquantel (PQZ) or iron
Table 3
Anthelmintic intervention studies investigating the impact of albendazole (ABZ) or mebendazole (MBZ) combined with iron supplementation on anaemia outcomes in non-pregnant populations
Table 2
Anthelmintic intervention studies investigating the impact of albendazole (ABZ) or mebendazole (MBZ) combined with praziquantel (PQZ) on anaemia outcomes in non-pregnant populations
Table 1
Anthelmintic intervention studies investigating the impact of albendazole (ABZ) or mebendazole (MBZ) on anaemia outcomes in non-pregnant populations

Across all 20 RCTs and observational studies, the mean change in Hb was higher in the treatment arm for all intervention packages than in the control arm: 2.3 g/l higher in the BMZ group; 3.7 g/l higher in the BMZ and PQZ group; 2.7 g/l higher in the BMZ and iron group; and 3.0 g/l higher in the BMZ, PQZ and iron group (Table 4). The effect of BMZ alone on mild and moderate anaemia was small (mean RR of 0.91 and 0.77), whereas the mean RR of BMZ plus PQZ was 0.72 for mild anaemia and 0.58 for moderate anaemia.

Eleven RCTs reported the effect of intervention on mean Hb with corresponding standard deviations (SD) or allowed their estimation (three studies did not report SDs: Awasthi et al. 2000; Stoltzfus et al. 2004; Nga et al. 2009). There was no overall effect of BMZ (SMD 0.05, 95%CI: −0.02 to 0.12), but looking at the drug effects separately, treatment with ABZ corresponded to a 1.89 g/l increase in mean Hb (SMD 0.15, 95% CI 0.01 to 0.29) whereas MBZ had no apparent impact (Figure 3a). Furthermore, combining ABZ and PQZ resulted in a 2.37 g/l increase in mean Hb (SMD 0.23, 95% CI 0.13 to 0.34) (Figures 3b). There was no evidence to support a beneficial impact of BMZ treatment when iron supplementation was co-administered in both arms of the trial (SMD 0.09, 95% CI −0.09 to 0.27) compared to neither arm (SMD 0.04, 95% CI −0.04 to 0.12).

Figure 3
Forest plot of the difference in the mean change in haemoglobin concentration (Hb) among individuals treated with an anthelmintic and individuals given a placebo in interventions studies (n= 10). Standardised mean difference greater than zero indicates ...

Among RCTs, treatment with BMZ alone had little impact on the risk of mild (RR 0.98, 95%CI: 0.89–1.06) and moderate (RR 0.87, 95%CI, 0.59–1.15) anaemia as determined by linear regression. When BMZ treatment was co-administered with PQZ, the mean relative risks of mild and moderate anaemia were 0.67 (95% CI −0.11 to 1.45) and 0.61 (95% CI 0.58–0.64). Among studies administering BMZ alone, a higher Hb at baseline was associated with a larger impact on moderate anaemia (P= 0.02), but there was no evidence of a differential impact when iron supplementation was co-administered in both arms of the trial (P= 0.69 and P= 0.63). No determinants of impact were identified for studies co-administering BMZ and PQZ.


The aetiology of tropical anaemia is complex, but the present systematic review confirms that hookworm infections of moderate or heavy intensity are associated with lower Hb levels in both school-aged children and adults (Layrisse & Roche 1964; Stoltzfus et al. 1997). The mechanisms by which hookworms reduce Hb are well established: adult worms attached to intestinal villi and pass a stream of blood through their intestines to obtain oxygen and nutrients. Fortunately, however, the current results show that anthelmintic treatment is an effective means of improving Hb levels, but that the effects of treatment appear to differ according the benzimidazole drug used (Figure 3). Among the included RCTs, treatment using albendazole was associated with an 1.89 g/l increase in Hb, whereas mebendazole treatment afforded no apparent benefit. However, the impact of benzimidazole treatment on Hb is enhanced by the co-implementation of praziquantel treatment and iron supplementation: for example, the addition of praziquantel resulted in a 2.37 g/l increase.

WHO currently recommends that school-aged children living in areas of high prevalence of soil-transmitted helminths (hookworms, Ascaris lumbricoides and Trichuris trichiura) receive mass treatment with either albendazole or mebendazole (WHO, 2002). Whilst these drugs are both highly efficacious against A. lumbricoides, with demonstrable gains for growth and school performance (Taylor-Robinson et al. 2007; Bundy et al. 2009), single-dose mebendazole treatment is less effective against T. trichiura and hookworm (Keiser & Utzinger 2008). Thus, the differential impact of albendazole and mebendazole on Hb levels can be explained in part by their varying efficacies against hookworm, although it should be noted that observed cure and egg reduction rates of mebendazole against hookworm vary among populations (Keiser & Utzinger 2008). However, this review identified only three published RCTs that investigated the impact of mebendazole on Hb, making it difficult to draw difficult definite conclusions about the effect of mebendazole in different hookworm-endemic regions of the world.

In areas co-endemic with schistosomiasis, benzimidazole is typically co-implemented with praziquantel. Previous attempts to quantify the haematological benefits of praziquantel have been hindered by the lack of RCTs evaluating the effects of PQZ alone (Friedman et al. 2005). Praziquantel has a direct effect against schistosomes, which may cause anaemia through a variety of proposed mechanisms, including extra-corporeal blood loss, sequestration of red blood cells, haemolysis and inflammation (Friedman et al. 2005). A previous systematic review of schistosomiasis-related morbidity (King et al. 2005) identified five studies evaluating the impact of praziquantel on Hb levels in hookworm-endemic areas; but two of these trials co-administered benzimidazole or metrifonate treatment. Metrifonate is partially effective against hookworm infection (Kurz et al. 1986), and therefore, inclusion of studies using metrifonate would potentially overestimate the impact of praziquantel treatment. A large multi-centre RCT of praziquantel and albendazole included by King et al. (2005) found that only treatment with praziquantel had an impact on Hb, but the estimate of impact for albendazole was not reported (Olds et al. 1999).

One of the difficulties of attributing effects of hookworm and anthelmintic treatment on anaemia, particularly among populations exposed to malaria and with inadequate dietary intake, is the exclusion of other causes. The multiple aetiologies of anaemia can confound cross-sectional estimates of association and influence the observed impact of anthelmintic treatment. Malaria (both symptomatic and asymptomatic) is an important aetiological factor for anaemia operating through several mechanisms including increased destruction of red blood cells (RBCs) through rupturing, phagocytosis and hypersplenism and reduced RBC production through inflammation and dyserythropiesis (Kurtzhals et al. 1999; Menendez et al. 2000; Tolentino et al. 2007). Extensive geographic overlap of hookworm and malaria yields a high prevalence of co-infection, which may increase in an additive manner the risk of anaemia (Brooker et al. 2007b). A further aetiological factor for anaemia is schistosomiasis, and co-infection with schistosomes and hookworm has been associated with enhanced anaemia risk (Ezeamama et al. 2008; Stephenson et al. 1985; Brito et al. 2006). In the present review, cross-sectional studies did not report adjusted intensity-stratified estimates of Hb and only 5 RCTs stratified results by nutritional status. Studies reported conflicting results: some found a differential impact based on anaemic status (Beasley et al. 1999) and intensity of hookworm infection (Stoltzfus et al. 1998; Adams et al. 1994), while others found no difference in impact between these groups (Taylor et al. 2001; Friis et al. 2003). Among children with S. mansoni infections, Friis et al. 2003 reported a greater impact on Hb associated with malaria co-infection, suggesting that malaria may influence the impact of schistosomiasis treatment.

A further potentially confounding factor is anthelmintic treatment efficacy. Reported estimates of the impact of benzimidazole treatment may underestimate the true magnitude of association because of incomplete treatment cure and the potential reinfection that occurs during follow-up (Bradley et al. 1993; Stoltzfus et al. 2000). Reinfection dynamics of helminths are well described and depend on a number of factors that vary between populations, including transmission intensity, efficacy of treatment and treatment coverage (Anderson & Schad 1985). Studies of hookworm reinfection support the view that prevalence and intensity of hookworm infection can return to pre-treatment levels within 1–2 years, with reinfection fastest in areas of high transmission and where treatment efficacy and coverage is lowest (Quinnell et al. 1993; Schad & Anderson 1985; Reynoldson et al. 1997 & De Clerq et al. 1997). A related issue is variation in follow-up time of included studies because a longer follow-up will allow more opportunities for reinfection and may therefore underestimate the impact of treatment on haemoglobin.

Diagnostic uncertainty may introduce additional bias. The dominant hookworm species present as well as the haemoglobin and diagnostic methods may influence observed impact. Few of the included studies distinguished between the two hookworm species N. americanus and A. duodenale because of the practical difficulties of differential diagnosis. However, it is suggested that A. duodenale causes greater blood loss than N. americanus (Pawlowski et al. 1991), with data from Zanzibari schoolchildren indicating that A. duodenale is associated with an increased risk of anaemia (Albonico et al. 1998). Finally, although Hb is routinely assessed as a measure of iron stores, it is insensitive to significant (20–30%) decreases in iron stores from higher Hb levels and is not specific to iron-deficiency anaemia (Zimmermann 2008). Other indicators of iron stores might provide a more sensitive measure of baseline nutritional status and influence the observed impact.

In conclusion, this systematic review confirms the benefits of anthelmintic treatment for improving Hb levels of infected populations but highlights important differences according to the type of benzimidazole drug used and the package of interventions treatment is combined with. This finding highlights the need for continual evaluation of the beneficial effects of deworming on Hb, with randomised evaluations providing the most robust evidence. However, there is also a need for rigorous, long-term evaluation of large-scale control programmes to ensure that they are having maximal benefits for the targeted populations. This would avoid denying individuals the benefits of treatment through randomised evaluations. The results additionally emphasise the public health benefits of combing health interventions and are of particular relevance to efforts implementing an integrated school health package, which may include deworming, iron supplementation, school feeding and malaria control (Bundy et al. 2006).


This work was supported by the Albert B. Sabin Vaccine Institute’s Human Hookworm Vaccine Initiative (HHVI) funded by the Bill and Melinda Gates Foundation, which also supports JLS. SB is supported by the Wellcome Trust through a Career Development Fellowship (081 673) and acknowledges the support of the Kenyan Medical Research Institute. We are grateful to Naufil Alam for helping conduct the initial literature review and to John Horton and Peter Hotez for constructive comments on an earlier draft.


1Only two studies among pre-school children (Stoltzfus et al. 2000; Brooker et al. 1999) were identified and therefore no further analysis among this age group was undertaken.

Supporting Information

Additional Supporting Information may be found in the online version of this article:

Table S1. Baseline characteristics and references of cross-sectional surveys included in the analysis of the impact of hookworm infection on haemoglobin concentration in non-pregnant populations.

Table S2. Quality assessment of randomised controlled trials investigating the impact of benzimidazole treatment on haemoglobin

Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.


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