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Detecting aggregated amyloid peptides (Aβ plaques) presents targets for developing biomarkers of Alzheimer's disease (AD). Polymeric n-butyl-2-cyanoacrylate (PBCA) nanoparticles (NPs) were encapsulated with radiolabelled amyloid affinity 125I-clioquinol (CQ, 5-chloro-7-iodo-8-hydroxyquinoline) as in vivo probes. 125I-CQ-PBCA NPs crossed the BBB (2.3 ± 0.9ID/g) (P < .05) in the WT mouse (N = 210), compared to 125I-CQ (1.0 ± 0.4ID/g). 125I-CQ-PBCA NP brain uptake increased in AD transgenic mice (APP/PS1) versus WT (N = 38; 2.54 × 105 ± 5.31 × 104DLU/mm2; versus 1.98 × 105 ± 2.22 × 104DLU/mm2) and in APP/PS1/Tau. Brain increases were in mice intracranially injected with aggregated Aβ42 peptide (N = 17; 7.19 × 105 ± 1.25 × 105DLU/mm2), versus WT (6.07 × 105 ± 7.47 × 104DLU/mm2). Storage phosphor imaging and histopathological staining of the plaques, Fe2+ and Cu2+, validated results. 125I-CQ-PBCA NPs have specificity for Aβ in vitro and in vivo and are promising as in vivo SPECT (123I), or PET (124I) amyloid imaging agents.
Alzheimer's disease (AD), a neurodegenerative disorder principally of the elderly, is the most prevalent form of dementia. The cognitive decline associated with AD drastically affects the social and behavioral skills of patients living with this disease. Notwithstanding the social impact, AD, also places great financial burdens on patients, families, and the community as a whole. Furthermore, therapeutic strategies to probe the central nervous system (CNS) are limited by the restrictive tight junctions at the endothelial cells of the blood brain barrier (BBB). To overcome the impositions of the BBB, polymeric biocompatible drug carriers have been applied to the CNS for many applications, including cancers; however, the field of drug carrier technology is not well developed in AD research . Polymeric nanoparticles are promising candidates in the investigation of AD since they are capable of opening tight junctions  crossing the BBB , high drug loading capacities, and targeting the mutant proteins of Alzheimer's [4, 5].
Markedly elevated concentrations of zinc, copper, and iron in Aβ deposits of the AD brain are well documented in the literature [6, 7]. Cherny et al. have employed the antibiotic and Cu/Zn-selective metal chelator, CQ, to inhibit Aβ accumulations in AD APP2576 transgenic (Tg) mice. Oral treatments with CQ reduced Aβ deposition by ≈49% and produced no neurotoxicity in a blinded study. The authors  speculate that CQ's action on the peptide may facilitate H2O2 inhibition . Moreover, the authors validated the selectivity of CQ since systemic metal depletion was not found ; CQ does not deplete brain tissue of metals, but rather binds to the Aβ-metal complex itself.
In in vivo experiments with nontransgenic animals treated with CQ, Yassin et al. confirmed significant decreases in the cerebral concentrations of Cu, Zn, and Fe metal ions . However, in Cherny's studies of APP2576 Tg mice, the inhibition of Aβ deposition by CQ caused significant increases in the cerebral concentrations of Cu and Zn . This is because APP transgene expression reduces Cu and Zn levels in vivo, while the Aβ concentration steadily rises . There are a few possibilities for the decrease. For example, the metal ions may be effluxed by Aβ, or APP/Aβ may prevent reuptake of the ions . Despite the mechanism, CQ prevents uptake; of the metals by the protein. This action affords metallic access to peripheral brain tissue, and the metal ion concentration increases. Since AD is a syndrome of metal dyshomeostasis, CQ may be able to restore the metal metabolism to its normal state.
Opazo et al.  have explored the Aβ-plaque binding properties of radioiodinated CQ in APP2576 Tg mice ([125I]CQ; higher brain retention compared with controls on autoradiography), and in AD patients ([123I]CQ; more rapid uptake compared with age-matched healthy controls). SPECT imaging was limited due to low tracer uptake, however, CQ was shown to localize with Aβ pathology . Notably, the authors  synthetically precipitated Aβ1–40 and Aβ1–42 by in vitro methods. The synthetic β-amyloid demonstrated 125ICQ saturation, which was directed by Zn2+; the 125ICQ binding was displaced by the heavy metals Zn2+ and Cu2+, the metal chelator DTPA, and the amyloid affinity dye Congo red. Enhancement of Zn2+-125ICQ was also shown in concentrated fractions of Aβ from postmortem AD brain. These results validate the effectiveness of CQ as a β-amyloid detection agent, and most importantly, correlate literature evidence linking Aβ deposition in AD and cerebral heavy metals [5, 8].
Our preliminary studies with 125ICQ showed that the agent crossed the BBB, but was retained too briefly for effective chelation. Therefore, a drug carrier is required to improve the extravascular retention of 125ICQ. Polymeric butylcyanoacrylate (PBCA) nanoparticles (NPs) were chosen as the drug carrier. Here, we report that 125I-CQ-BCA NPs act as targeted drug carriers with an affinity for amyloid plaques. 125I-CQ-PBCA NPs are promising candidates for in vivo brain imaging of the amyloid plaques.
CQ was radiolabelled with 125I (Perkin Elmer, Waltham, MA) by the Chloramine- T (CT) method of radioiodination. Purification of 125I-CQ was performed by solvent extraction in dichloromethane (DCM) and H2O. The organic layer (e.g., DCM) was evaporated under N2 gas, and the 125I-CQ was dissolved in dimethylsulfoxide (DMSO, 1mL). The purification of 125I-CQ was analyzed by thin layer radiochromatography (RTLC). The specific purity was >95%.
The NPs were polymerized as per the modified procedure of Kreuter et al. . A polymerization medium was prepared containing Dextran 70000 and Tween-80 (polysorbate 80) (both at a concentration of 1% each in 0.1NHCl) (Sigma, USA); 5.05 × 106Bq 125I-CQ was added to the solution just prior to the addition of butylcyanoacrylate (BCA) monomer. A 1% (w/v) butylcyanoacrylate solution (Sichelwerke, Hannover, Germany) was added drop wise during constant magnetic stirring at 400rpm. After 3 hours of polymerization, the NP suspension was neutralized with 0.1NNaOH to complete the polymerization. This solution was filtered with a 0.2μm filter and purified by ultracentrifugation (Beckman Coulter, Fullerton, CA; 45Krpm, 1h). The pellet was washed and redispersed in water, which contained 1% Tween-80. The PBCA nanoparticles were then overcoated with 1% Tween-80 by stirring for 30 minutes in phosphate buffer solution (1X PBS), just before in vivo administration. Nanoparticle size (45nm) was determined by a Zetasizer 3000HS (Malvern, Worcestershire, UK).
Cortical frontal AD and control brain tissue were obtained from the tissue bank, as approved by our Institutional Review Board (800μL buffer solution of 0.1% FBS) in the presence of 125I-CQ (1.17 × 104Bq in 100μL PBS). Brain homogenates were microcentrifuged (13KRPM, 15 minutes) and the percent binding was calculated. The experimental results provided evidence of preferential binding by 125I-CQ to the AD brain tissue, as compared to cortical control brain tissue.
Amyloid protein (1–42), Aβ42, (0.5mg) was purchased from Bachem California (Torrance, CA), and dissolved in 1.15mL PBS (pH 7.4) to a final concentration of 435μg/mL (100μM) by magnetically stirring the solution in a closed vessel at 1200RPM for 7 days at room temperature . After 7 days, the aggregated peptide suspension was visibly cloudy. The aggregated Aβ42 was stored in 100μL aliquots at −20°C until use.
All use of animals was in compliance with the regulations of the Animal Resources Center (ARC) of UT Southwestern Medical Center, and approved by the Institutional Review Board (IRB). All mice were anesthetized intraperitoneal (IP) with 100–150μL of Ketamine HCL (Sigma, St. Louis, Mo). Wild type BALB/C mice (Charles River Laboratories, Wilmington, MA; N = 17) were injected by direct stereotaxis (Model 900 Small Animal Stereotaxic Instrument, David Kopf Instruments, Tujunga, CA) with the aggregated Aβ42 peptide at a concentration of 1μg/1μL, and at a constant flow rate of 60 seconds. A lubricant was placed into the eyes of the mice to prevent over-drying during the experiments. The mice received unilateral injections of either saline or the Aβ peptide aggregate. The injection location corresponded to the mouse brain hippocampus structure CA1, at coordinates −1.5, −1.0, and −1.8, relative to Bregma [15, 16]. The peptide was allowed to grow for 7 days, during and after which time cognitive tests for behavior (Y-maze) were performed.
The 125I-CQ-BCA NPs (1–3mg) were administered to AD mouse transgenic models by lateral tail vein injection. The Aβ42 mice received 125I-CQ-BCA NPs at 8 days postinjection of the peptide.
In vivo Storage Phosphor autoradiography (Perkin Elmer Cyclone Storage Phosphor Imaging System; OptiQuant Imaging Software) was used to determine the relative qualitative differences in the brain uptakes of 125I-CQ by the AD mouse models and wildtype control mice. Briefly, each mouse was anesthetized I.P. with 100–150μL of Ketamine HCl (Sigma Aldrich, USA) throughout the duration (5–90 minutes) of the imaging experiment. Postinjection of the radiotracer, the mouse was placed on the phosphor screen with a lead sheet between the film and the animal's body in such a way that the only exposed body part was the head. In this way, background radiation from the body was minimized, and the activity source projected onto the film was from the animal's head region only. Regions of interest (ROI) were drawn in the brain space to obtain information in semiquantitative units (Dynamic Light Units, DLU) per volume space (DLU/mm2).
At the conclusion of the imaging experiments, the mice were sacrificed by cardiac perfusion through the left ventricle with 4% paraformaldehyde. The brains were harvested and stored in formalin, before being embedded in wax, and sectioned at a 5μm slice thickness. The slices were stained with Congo red (amyloid plaques), Prussian blue (Fe2+), and Rubeanic acid (Cu2+).
Mice with a double mutation (APP/PS1) for Alzheimer's disease were commercially purchased (N = 5) from The Jackson Laboratories (Bar Harbor, ME; strain B6C3-Tg(APPswe, PSEN1dE9)85Dbo/J). This particular mouse model corresponds to a form of early onset disease and expresses a mutant human presenilin 1 and a chimeric mouse/human amyloid precursor protein (APPSwe). The expression of both transgenes was directed by the mouse prion protein promoter. The APPswePS1 strain was developed on a B6C3HF2 background. The chimeric APP was modified to encode the Sweedish mutations K595N/M596L in order to elevate the amount ot Aβ produced from the transgene, by favoring processing through the beta-secretase pathway. Mice with the double mutation (APP/PS1) were generously donated (N = 33) by Dr. David Russell (UT Southwestern). Mice with the triple mutation (APP/PS1/Tau) were generated (N = 2) by Dr. Malu Tansey (UT Southwestern), to express the knock-in human presenilin 1 mutation, mutant Tau, and the Swedish APP mutation.
Data were entered into Excel worksheets (Microsoft Corporation, Redmond, WA), and analyzed using the non-paired, two-tailed Student's t-test with unequal variance. P < .05 was regarded as significant.
125ICQ was used in in vitro assays of human postmortem frontal cortex to test the affinity of the radiolabelled chelator for amyloid plaques. Age-matched normal specimens were tested as control samples; no AD pathology was found in normal tissue (i.e., plaques and tangles in the brain). Experimental results (Figure 1) provide evidence of preferential binding by 125I-CQ to the AD brain tissue (1000μg, 18.5% binding), as compared to cortical control brain tissue (1000μg, 13% binding). Therefore, an amyloid-affinity drug could be successfully radiolabelled; the radioligand discriminated between AD brain tissue and control brain tissue.
The BCA monomer was polymerized with small particulate diameter (45nm) and with uniform size distribution (Figure 2). In vivo biodistribution experiments showed that the free 125I-CQ had a rapid brain uptake, as well as rapid blood clearance in normal mice (Table 1). Furthermore, 125I-CQ cleared the brain quickly; the % ID/g for the brain at 2 minutes was 0.99 ± 0.40%, and at 4 hours, it was 0.04 ± 0.02%. Table 2 shows that when the 125I-CQ was encapsulated within the BCA NPs, the brain uptake was enhanced. At two minutes, the wild type mice exhibited 2.31 ± 0.89% uptake of the 125I-CQ BCA NPs in the brain (Table 1). Brain and blood clearances of the 125I-CQ BCA NPs were rapid; the % ID/g (brain) at 4 hours was 0.02%. Together, Tables Tables1 and1 and and2 show2 show that 125I-CQ BCA NPs have an increased brain uptake versus 125I-CQ in the wild type control mouse.
Table 3 shows similar results, in Dynamic Light Units/mm2 (DLU/mm2), the unit described in autoradiographic imaging. For example, in the wildtype control mouse, at 10 minutes post injection the brain uptake of encapsulated 125I-CQ is 1.64 × 105 ± 1.75 × 104 versus 2.26 × 105 ± 4.22 × 105 in the AD transgenic mouse. Moreover, the brain uptake is significantly greater in the AD mouse at 90 minutes post injection of 125I-CQ BCA NPs (1.98 × 105 ± 2.22 × 104 wildtype versus 2.54 × 105 ± 5.31 × 104 AD transgenic)
Triple transgenic mice, for which mutations in the APP and Tau genes result in amyloid plaques and neurofibrillary tangles, were tested against wildtype controls for brain uptake of 125I-CQ BCA NPs. The in vivo uptake was imaged by autoradiography, and the data are given (DLU/mm2) in Table 4. Table 4 shows that at both 60 minutes and 90 minutes post injection of 125I-CQ BCA NPs, the AD transgenic mouse had a significantly greater brain uptake and retention of the imaging agent, as compared with the wildtype control. For example, at 60 minutes post injection, the brain uptake in the AD mouse was 6.28 × 106 ± 1.68 × 106 versus 3.92 × 106 ± 1.49 × 106 in the wildtype control.
The PBCA NPs were successfully loaded with the radiolabelled quinoline derivative 125I-CQ and delivered to the mice by intravenous administration. Storage Phosphor imaging qualitatively showed that the NPs transported the drug across the BBB. Further, the 125I-CQ labeled the amyloid deposits.
Figure 3 shows in vivo autoradiographs of the brain uptakes of 125ICQ BCA NPs and 125ICQ in transgenic mice (7 months old, APP/PS1), at 15 minutes postadministration of the nanoparticle-encapsulated drug and the free drug. The AD transgenic mice have a greater brain uptake with the use of nanoparticles, as compared to the free 125ICQ; thus, nanoparticulate encapsulation of 125ICQ enhances BBB crossing of the drug. Furthermore, nanoparticulate encapsulation of 125ICQ enhances retention of the drug. Figure 4 shows in vivo autoradiographs of the brain uptakes of 125ICQ BCA NPs and 125ICQ in transgenic mice (7 months old, APP/PS1), at 90 minutes post administration of the nanoparticle-encapsulated drug and the free drug. At 90 minutes post injection, the AD transgenic mice have a greater brain uptake with the nanoparticles, as compared to the free 125ICQ. Therefore, transgenic mice have longer brain retention of the nanoparticle delivered drug, as compared to the free drug. Figure 5 shows in vivo autoradiographs of the brain uptakes of 125ICQ BCA NPs in a 12-month wild type control mouse, and in a 15-month AD transgenic mouse (APP/PS1). At one hour post injection, the AD transgenic mouse has an increased brain uptake of the nanoparticles, presumably due to the presence of amyloid plaques.
Experiments with 125ICQ BCA NPs were also done in AD triple transgenic (3 × Tg) mice (APP/PS1/Tau). In Figure 6, in vivo autoradiographs show that at one hour post injection, the AD triple Transgenic mouse had the higher brain uptake of the nanoparticles (as compared to the wild type control mouse). Additionally, a Bielchowsky stain (Figure 7) verified the AD pathology in the 3xTg mouse. Finally, histological staining of brain slices taken from these AD mouse models verified the presence of amyloid (Congo red, Figure 8), Fe2+ (Prussian blue, Figures Figures9 and9 and and11),11), and Cu2+ (Rubeanic acid, Figure 10).
Cyanoacrylate nanoparticles were specifically designed for carrying drugs across the blood-brain barrier in mouse models of Alzheimer's disease. In vivo biodistribution of the 125I-CQ-labelled butylcyanoacrylates in wild-type mice showed that they crossed the BBB with greater efficiency than the 125ICQ control. The BCA NP was selected as the prototype drug carrier because it polymerized with the most reproducibility; it crossed the BBB and had a rapid uptake and clearance from the normal brain. These parameters are important in order to validate the PBCA NP as a drug carrier across the BBB; another unique factor is the lipophilicity of the carrier. Good lipophilicity assists rapid uptake and clearance from the normal brain . The lipophilicity of the nanoparticles was enhanced by surfactant coating with Tween-80.
The prototype PBCA NP was fully characterized for physicochemical and stabilizer effect. Smaller sized particles resulted when the monomer was polymerized at a lower pH, in the presence of the stabilizer Dextran 70000 (as opposed to the hydrophilic polyethylene glycol, PEG), and definitely in the presence of a surfactant (e.g., Tween-80). Loading of the nanoparticles with amyloid-affinity drugs, such as derivatives of the Thioflavins (S- or T-), or Congo red did not significantly affect the size of the BCA NPs. Therefore, the BCA NPs maintained stability upon drug loading in vitro. Additional amyloid-affinity drugs have been reviewed in the literature [2, 17, 18].
The amyloid-affinity chelator CQ was successfully radioiodinated with 125I; 125ICQ was used in in vitro assays of human postmortem frontal cortex to test the affinity of the radiolabelled chelator for amyloid plaques. Autoradiography validated preferential labeling of the AD tissue by the 125ICQ (compared with control brain tissue). Then, the 125ICQ was successfully encapsulated within PBCA NPs. 125ICQ PBCA NPs preferentially labeled frontal human AD tissue compared with frontal control tissue. PBCA NPs act as drug carriers of 125ICQ targeted towards amyloid-beta plaques, presumably due to chelation of transitional metals in amyloid plaques.
In vivo detection of amyloid plaques for the early diagnosis of AD is desirable. Presently, histological confirmation of the plaques and of the neurofibrillary tangles is the only definitive mode of diagnosis. This is true despite the fact that patients typically receive clinical diagnoses based on cognitive tests, medical histories, and so forth. Noninvasive in vivo detection affords patients the opportunity to receive the most effective patient care as early as possible. Likewise, it allows clinicians the prospect of tracking disease progression when definitive treatment becomes available. Hence, healthcare professionals are able to design appropriate therapeutic strategies.
In vivo detection of amyloid-beta proteins improves specificity of diagnosis in noninvasive screening techniques, such as single photon emission computed tomography (SPECT) imaging. We have designed polybutylcyanoacrylate nanoparticles with incorporated radioligands and amyloid affinity agents that are attracted to the Aβ proteins. Thus, the clinical potential of the 125ICQ-BCA-NPs is improved specificity of diagnostic accuracy in AD detection.
The authors would like to thank Tejraj Aminabhavi, Ph.D., David W. Russell, Ph.D., Charles White, M.D., Michael Bennett, M.D., Hilary Wilson, M.S., Perry N. Fuchs, Ph.D., Rebekkah Warren, B.S, John Shelton, B.S., and Thomas S. Harris, M.S.. This work was supported by NIH NIGMS 1 F31GM066381.