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Neutrophils have a dual affect on epithelial pIgR/SC, the critical receptor for transcellular routing of mucosal IgA, but mechanisms of pIgR/SC upregulation remain elusive. Requirements of cytokine, redox, and signalling pathways for pIgR/SC production were assessed in human bronchial epithelial (Calu-3) cells cocultured with increasing numbers of blood neutrophils. Increased SC production was observed after incubation for 48hrs with intermediate neutrophil numbers (1.25 to 2.5 × 106), was favoured by the elastase inhibitor SLPI, and correlated with increased TGF-β production. Exogenous TGF-β stimulated SC production with a maximal effect at 48hrs and both TGF-β- and neutrophil-driven SC upregulation were dependent on redox balance and p38 MAP-kinase activation. This paper shows that activated neutrophils could upregulate epithelial pIgR/SC production through TGF-β-mediated activation of a redox-sensitive and p38 MAPK-dependent pathway. An imbalance between the two neutrophil-driven opposite mechanisms (SC upregulation and SC degradation) could lead to downregulation of pIgR/SC, as observed in severe COPD.
The mucosal surfaces lining the respiratory tract are continuously exposed to potentially infectious agents, as well as environmental toxics and antigens. The bronchial epithelium acts as a physicochemical barrier of the airway mucosa and is able to initiate inflammatory responses by releasing mediators upon exposure to stimuli such as cigarette smoke- or pathogen-derived molecules. One first-line mechanism of the airway immune defense against inhaled antigens and microorganisms consists of secretory immunoglobulin A (SIgA), the predominant immunoglobulin isotype in mucosal secretions [1, 2]. In the lamina propria, mucosal plasma cells produce IgA molecules mainly as polymers, linked through a joining chain. Polymeric IgA (pIgA) then binds to the polymeric Ig receptor (pIgR), a transmembrane glycoprotein selectively expressed on the basolateral surface of epithelial cells. The pIgA-pIgR complexes are transported across epithelial cells up to the apical membrane where a proteolytic cleavage releases the main part of the extracellular domain of pIgR, known as secretory component (SC), remaining bound to pIgA to form SIgA  which assumes protective, antiinfectious functions within the mucosal lumen.
Chronic obstructive pulmonary disease (COPD) represents the fourth leading cause of death worldwide and results in large consumption of health care resources . This disorder characterized by progressive and mostly irreversible limitation of expiratory airflow  is mainly caused by cigarette smoking, whereas only some (15–20%) smokers will develop the disease. Among features of COPD are epithelial changes (mucous hyperplasia) and chronic inflammation with recruitment/activation of leukocytes including neutrophils, macrophages, and CD8+ T cells . However, the links between airway neutrophilic inflammation and impairment of epithelial functions remain poorly understood in COPD. We previously showed that expression of pIgR/SC was strongly decreased in the bronchial epithelium from patients with severe COPD, and that this epithelial defect correlated with airflow limitation and with infiltration of submucosal glands by neutrophils .
Neutrophils can be involved in various lung disorders notably through the release of oxidants, proteinases (including serine proteinases) and defensins, which have important effects on several epithelial functions such as mucus secretion, cytokine release, or receptor expression. More specifically, we next showed that activated neutrophils can induce in vitro a cleavage of epithelial pIgR/SC through the release of serine proteinases (particularly neutrophil elastase), whereas a stimulatory effect on pIgR/SC production by cultured bronchial epithelial cells was also observed . However, the mechanisms regulating this opposite effect of neutrophils on pIgR/SC expression in airway epithelial cells remain unclear, in particular factors responsible for increased pIgR/SC synthesis upon stimulation by neutrophils are not identified.
The aim of the present work was therefore to evaluate the molecular determinants of the “dual effect” of neutrophils on pIgR/SC expression in human bronchial epithelial cells. More particularly, the involvement of soluble mediators in the epithelial pIgR/SC upregulation by neutrophils was investigated, as well as the role of proteinase/antiproteinase and redox balances and of intracellular MAPKinase signaling pathways.
Calu-3, a human (adenocarcinoma) bronchial epithelial cell line, was obtained from the ATCC (Rockville, MD), and cultured in RPMI-1640 supplemented with 10% decomplemented fetal bovine serum, 100IU/ml penicillin, 100μg/ml streptomycin and 2mM L-glutamine (Cambrex, Rockland, ME) and referred to as “complete medium”. 96-well plates were precoated for 2hrs at 37°C, with 3.1μg/ml vitrogen (Cohesion, Palo Alto, CA), 10 μg/ml fibronectin, and 50μg/ml bovine serum albumin in RPMI and followed by two washes with RPMI. To obtain a confluent bronchial epithelial layer, 50,000 Calu-3 cells were seeded in this 96-well plate in 200μl of complete medium and cultured for 5–7 days until confluence.
Polymorphonuclear neutrophils were obtained from whole heparinized blood from healthy donors by a one-step density gradient method (40min centrifugation, 800×g, room temperature) using Polymorphprep (Axis Shield, Oslo, Norway) following manufacturer's protocol. After washing, neutrophils were resuspended in RPMI-1640 and activated by 20 ng/ml IL-8 (R&D System, Minneapolis, MN) for 5 min followed by 1nM formyl-Methionyl-Leucyl-Phenylalanine (fMLP) (Sigma Aldrich, Saint Louis, MO) for 30min at 37°C . In all experiments, IL-8/fMLP activated neutrophils were represented by “activated neutrophils”.
Activated neutrophils alone were incubated (from 1.25 to 5 × 106 cells) in complete medium for 48hrs to determine the production of cytokines including TGF-β1 in supernatants.
Confluent Calu-3 bronchial epithelial cells were cultured for 48hrs, with or without TGF-β1 (20ng/ml, R&D System). To evaluate the dose response to TGF-β1, cells were incubated with increasing concentration from 0.2 to 80ng/ml. After culture, pIgR/SC production was measured in supernatants and cell lysates.
Calu-3/neutrophils coculture. To analyse the dose–response effect of neutrophils on SC production by bronchial epithelial cells, confluent monolayers of Calu-3 cells were incubated for 48hrs with increasing numbers of activated neutrophils (from 0.3 to 15 × 106 cells) in the presence or not of 2.5μM human Secretory Leukocyte Protease Inhibitor (SLPI, R&D system).
Kinetics (from 2 to 72hrs) of TGF-β1 production by Calu-3 epithelial monolayers alone, activated neutrophils (5 × 106 cells) alone or bronchial epithelial cells cocultured with neutrophils was also analyzed. Similarly, kinetics (from 1 to 48hrs) of SC secretion by Calu-3 epithelial layers was measured after incubation with TGF-β-1 (20ng/ml) or activated neutrophils (5 × 106 cells).
At the end of each culture, the required volume of supernatant was taken to measure elastase enzymatic activity, and 2mM Phenylmethylsulfonyl fluoride (PMSF, Sigma Aldrich) was then added to inhibit further serine proteinase activity. Supernatants were harvested and frozen at −20°C until SC titration. Epithelial cells were washed twice in PBS and lysed with 100μl Triton (Sigma Aldrich) 0.1% w/v in PBS for 15min on ice. Cell lysates were harvested and frozen at −20°C until SC titration.
Independent experiments represent results from different neutrophil donors.
Cell viability and activity of mitochondrial electron transport chain, as indicator for cytotoxicity, was determined by the capacity of cells to reduce MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) to formazan . Confluent Calu-3 epithelial cells were incubated with increasing number of neutrophils (from 0.3 to 15 × 106), with increasing concentrations of TGF-β (from 0.2 to 80ng/ml), with CSE (from 0.1% to 10%) and with MAPKinases inhibitors (PD, SP, and SB) for 48hrs. After incubation, cells were washed with PBS. 100μl of PBS containing 1mg/ml MTT was added to each well and the plate was returned to 37°C. After 1h, the medium was removed by flicking off. To solubilize intracellular formazan produced by active mitochondria in living cells, 100μl of dimethylsulfoxide (DMSO) was added per well. The absorbance at 550nm was measured on a spectrophotometric plate reader and used for the calculation of relative cell viability where untreated cells represented 100% viability.
SC concentration was determined in culture supernatants and cell lysates from epithelial cells by sandwich ELISA, as previously reported , by using affinity purified goat antihuman SC polyclonal antibody (developed in our laboratory, recognizing both soluble SC and membrane pIgR/SC) as capture and detection (biotinylated) antibody. The reaction was revealed by streptavidine-HRP followed by tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. OD were recorded at 450nm using a microplate spectrometer (Biorad). Sensitivity of the immunoassay was ~0.2ng/ml.
TGF-β production was measured in supernatants from Calu-3 cells, neutrophils or Calu-3/neutrophils cocultures by ELISA. A step of sample extraction (acidification) was carried out to allow for the release of TGF-β1 from latent complexes, making it accessible for measurement in the immunoassay. A monoclonal antibody specific for TGF-β1 had been coated onto the wells and extracted samples and standards were put into these wells followed by addition of biotinylated secondary antibody (TGF-β immunoassay kit, Biosource, Nivelles, Belgium). The sensitivity of TGF-β1 immunoassay was 15.6pg/ml.
Neutrophil elastase activity was measured in epithelial cell/neutrophils coculture supernatants. N-methoxysuccinyl-ala-ala-pro-val-p-nitroanilide (Sigma Chemical) was used as specific substrate, diluted at 1mM in 0.1M HEPES buffer, pH 7.5, with 0.5M NaCl and 10% v/v dimethylsulfoxide. Supernatants (50μl) were incubated with 100μl of substrate solution at 37°C in 96-well plates, and OD increase was monitored at 415nm using a microplate reader (Biorad). Results were compared with standard curves obtained with serial dilutions of purified neutrophil elastase (Elastin products Co., Owensville, MA), and expressed in nmole/l.
Confluent bronchial epithelial layers were incubated for 48hrs with TGF-β1 (20ng/ml) or with PMN (5 × 106) in the presence of the antioxidants N-acetylcystein or glutathione (Sigma) at final concentration of 10mM. The production of SC was measured in supernatants.
After 30min of stimulation by TGF-β of a confluent monolayer in a 12-well plate, Calu-3 cells were lyzed in 150μl of RIPA buffer [16mM Tris base, 150mM NaCl, 6mM EDTA, 10g Nonidet (NP-40), 12mM sodium deoxycholate, 3mM SDS, and pH 7.4] with 10μg/ml of protease cocktail inhibitor (Roche), 1mM PMSF, 1mM NaF and 1mM Na3VO4. 40μl of protein lysate are diluted in 40μl of Laemmli's sample buffer, heated at 100°C for 5min, loaded in a 12% SDS-PAGE gel and run at 180V for 1h. Cell proteins were transferred onto a nitrocellulose membrane (Hybond-C, Amersham Biosciences, UK) at 70mA for 1.5h at room temperature. The membrane was blocked with 5% BSA in Tris-buffered saline with 0.1% Tween 20 (TBST) for 1h at room temperature, washed, and incubated with the primary Ab diluted 1/1000 in TBST-BSA 1% (anti-phospho ERK, anti-phospho p38 or anti-phospho JNK; Cell signalling Technology, Beverly, MA) overnight at 4°C. Membranes were then incubated for 1h at room temperature with a secondary Ab diluted 1/2000 in TBST-BSA 1% (anti-rabbit IgG/HRP, Cell signalling Technology). Immunoreactive bands were developed using chemiluminescence (ECL, Amersham Biosciences, UK) and detected with the Chemidoc XRS apparatus (Bio-Rad Laboratories, Hercules, CA).
Calu-3 epithelial cells were pretreated for 1hr with 50μM PD98059, 50μM SB203580 or 50μM SP600125 as selective inhibitors of extracellular signal-regulated kinase-1/2 (ERK) mitogen-activated protein kinase (MAPK), p38 MAPK, and c-jun-N-Terminal Kinase (JNK) MAPK, respectively (New England Biolabs, Beverly, MA). Calu-3 cells were then incubated for 48hrs with TGF-β1 (20ng/ml) or neutrophils (5 × 106 cells), and production of SC in supernatants was determined by ELISA.
Results were expressed as mean ± SEM, unless otherwise stated. The differences between the groups were analyzed as appropriate by means of the Mann Whitney test, using Graphpad Prism 4.0 (Graphpad Prism, San Diego, CA). P values <.05 were considered as statistically significant.
To set up a model to examine effects of neutrophils on SC production by bronchial epithelial cells, confluent epithelial layers of Calu-3 cells were incubated for 48hrs with increasing numbers of activated neutrophils (Figure 1(a)), with evaluation of cytotoxicity. Neutrophil concentrations from 0.3 × 106 to 5 × 106 did not significantly affect epithelial cell viability according to MTT assay (viability ≥ 80% compared to control). However cytotoxicity was observed for ≥10 × 106 neutrophils (cell viability decreased by ~20%).
SC production increased when epithelial cells were incubated with neutrophils, starting at 1.25 × 106 and with a maximal increase at 2.5 × 106 neutrophils (P < .01, Figure 1(a)). At further increased neutrophil numbers (>5 × 106), SC production was downregulated and tended to decrease at 15 × 106 neutrophils as compared to control (P = .08, Figure 1(a)), consistent with cytotoxicity (MTT assay) and/or proteolytic cleavage . Elastase activity evaluated in supernatants from the same Calu-3/neutrophils cocultures increased as expected with the number of neutrophils (Figure 1(b)). These results indicated that this system allowed to address the two opposite effects of neutrophils on epithelial SC, and experiments were carried out to explore the mechanisms of SC upregulation by “nontoxic” numbers of neutrophils releasing elastase in a concentration range that could be observed in chronic airway neutrophilic diseases, for example, during exacerbations.
To evaluate the role of serine proteinases on the regulation of SC production by neutrophils, Calu-3 cells were cultured with increasing neutrophil numbers in the presence or not of secretory leukocyte protease inhibitor (SLPI), a natural inhibitor of neutrophil elastase, at a concentration (2.5μM) almost completely suppressing elastase activity (>90% inhibition). SC production was measured in both epithelial supernatants and cell lysates (Figure 2). In the presence of exogenous SLPI, SC upregulation observed in epithelial supernatants at intermediate neutrophil numbers (P < .05 at 2.5 × 106 neutrophils) was further increased. In addition, the presence of SLPI promoted an increase in pIgR/SC in epithelial lysates (P < .05 at 0.6 × 106 neutrophils), while no effect was observed in this cell compartment in the absence of SLPI (Figure 2) or with SLPI alone, without neutrophils (data not shown).
Besides the effect of proteinases/antiproteinases on SC upregulation, involvement of other neutrophil (and/or epithelial)-derived soluble factors was assessed. Cytokines known to induce pIgR transcription such as TNF-α or IL-1β were not detected in coculture supernatants (data not shown). In contrast, TGF-β, which was detected in resting Calu-3 cell supernatants at a similar level as in complete (serum-supplemented) medium, was increased upon neutrophil coculture (~3-fold increase at 5 × 106 neutrophils, P < .05; Figure 3(a)).
In order to determine the respective role of epithelial cells and neutrophils in TGF-β production, a time course of TGF-β production by epithelial cells alone, activated neutrophils alone or epithelial cell/neutrophil cocultures was carried out (Figure 3(b)). A trend for TGF-β increase was observed in neutrophil and coculture supernatants as early as after 2hrs, and further increased at later time points (P < .05 at 48 and 72hrs, Figure 3(b)) in cocultures. In contrast, epithelial cells began to secrete TGF-β between 48–72hrs, with a 3-fold increase as compared to 2hrs (P < .05). At each time point, TGF-β in neutrophil supernatants was higher than the epithelial production, except at 72hrs. These results indicated that neutrophils represented the main source of TGF-β up to 48hrs of coculture, with a modest (and late) contribution from epithelial cells.
TGF-β was then evaluated for a potential effect on pIgR/SC production. TGF-β had no significant cytotoxic effect on epithelial cell viability up to 80ng/ml, with even increased MTT reduction at 80ng/ml (not shown). Addition of recombinant human TGF-β1 significantly increased pIgR/SC production, in cell lysates (P < .05; Figure 4(a)), and a trend was also observed for SC secretion in supernatants. In dose-response experiments, SC production upon TGF-β stimulation increased from 1ng/ml to reach a plateau ~20ng/ml (Figure 4(b)). The kinetics of SC production by bronchial epithelial cells incubated with TGF-β or neutrophils, the main source of TGF-β, was assessed (Figure 4(c)). From 1hr to 14hrs of incubation, no difference was observed between the three conditions of stimulation, constitutive SC production starting to significantly increase from 24hrs. Effects of TGF-β and neutrophils had similar kinetics on SC production, with upregulation observed at 48hrs (Figure 4(c)).
We examined whether the redox balance could affect TGF-β- and neutrophil-induced SC upregulation. Calu-3 cells were cultured with TGF-β or neutrophils in the presence or not of two antioxidants, N-acetylcysteine and glutathione. In the presence of these antioxidants, effects of neutrophils were inhibited, with a trend for TGF-β (Figure 5(a)). N-acetylcysteine and glutathione had no effect on TGF-β production, whereas they did slightly reduce elastase activity presumably by reducing the pH (data not shown).
The link between p38 MAPK activation and redox balance in epithelial cells cocultured with activated neutrophils was assessed. Activation of p38 and ERK was assayed in Calu-3 cells cocultured with neutrophils in the presence or not of N-acetylcysteine (Figure 5(b)). Upon neutrophil coculture ERK was activated at 60min, while p38 was activated as soon as after 5min and also peaked at 60min. In the presence of the antioxidant, p38 MAPK activation was inhibited, whereas that of ERK was not affected or even promoted.
To analyse the intracellular mechanisms involved in SC upregulation and engaged in bronchial epithelial cells activated by TGF-β or neutrophils, activation of ERK, p38, and JNK MAPKinase was assessed, as well as effects of their selective inhibition. For each inhibitor, specificity and absence of significant cytotoxicity was confirmed by western blot and MTT assay, respectively (data not shown). Activation of ERK and p38 MAPK was confirmed in Calu-3 stimulated by TGF-β, with a maximal effect at 5min both for ERK and p38 (Figure 6(a)).
The pharmacologic inhibitors did not significantly affect baseline SC secretion (Figure 6(b)). While no significant effects were observed with ERK or JNK pathway inhibitors (PD98059 and SP600125, resp.), the p38 MAPK inhibitor (SB203580) suppressed neutrophil- and TGF-β-driven SC upregulation (P < .05, Figure 6(b)).
Epithelial pIgR/SC is the critical receptor for the transcellular routing of IgA involved in frontline defense mechanisms at mucosal surfaces, including the airways. We previously observed that the bronchial epithelium of severe COPD displays a strongly reduced pIgR/SC expression, which correlated with airway obstruction and neutrophil infiltration . Our previous in vitro study showed that neutrophils could induce on one hand pIgR/SC cleavage and on the other hand upregulation of SC production in epithelial cells , suggesting a “dual effect” of neutrophils on pIgR/SC. This study shows that, in an in vitro coculture system set up to address the mechanisms of pIgR/SC dual regulation by neutrophils, increased epithelial SC production was observed at intermediate numbers of neutrophils (corresponding to ~10nM elastase released), while further increasing neutrophils downregulated SC, presumably due to proteolytic cleavage (>20nM elastase; ) and/or cytotoxic effects. In addition, SC upregulation was favoured in the presence of SLPI, a natural elastase inhibitor, and associated with TGF-β production by neutrophils. Moreover, we show for the first time that TGF-β is able to stimulate epithelial pIgR/SC production, in a dose-dependent manner. We also demonstrate that TGF-β- and neutrophil-driven SC upregulation depends on the redox balance (cigarette smoke extracts and antioxidants both suppressing the effect) and that p38 MAP kinase represents a downstream, intracellular signalling pathway targeted by TGF-β to induce pIgR/SC production upon stimulation by neutrophils.
Neutrophils are able to secrete a large range of soluble factors including proteinases and oxidants, as well as cytokines/chemokines and growth factors, involved in inflammatory responses and tissue remodelling. Among these factors produced by neutrophils, our data indicate that elastase and TGF-β represent the major candidates to underlie, respectively, the down- and upregulation of pIgR/SC in epithelial cells exposed to activated neutrophils. A robust production of TGF-β was detected in our coculture supernatants, mainly arising from neutrophils. In addition, a dose-dependent increase in SC expression was observed upon exogenous TGF-β stimulation. TGF-β produced in cocultures ranged from ~0.5 to 1ng/ml (Figure 3), a concentration close to levels effectively upregulating SC production (Figure 4(b)). In addition, maximal SC production was reached at 48hrs of stimulation, both for TGF-β and neutrophils. Moreover, functional experiments pointed to common molecular pathways (redox system, p38 MAPK), further supporting that neutrophils could induce pIgR/SC expression mainly by producing TGF-β.
While elastase is the major serine proteinase released by neutrophils involved in lung tissue breakdown due to cigarette smoking (know as “emphysema”), TGF-β represents a multifunctional growth factor. It modulates cell proliferation and differentiation and induces synthesis of extracellular matrix proteins [10, 11] and is thereby involved in the development of tissue fibrosis. TGF-β has also been shown as chemotactic for neutrophils, while exerting prominent immunosuppressive functions . In addition, it has a unique role in mucosal tissues in promoting class switch recombination of mucosal plasma cells to IgA, a critical Ig for prevention of microbial infection and for control of the commensal flora [13, 14]. Here, we show that TGF-β not only triggers IgA synthesis but also promotes its transport across the epithelium, further supporting the view of this cytokine as a critical factor in mucosal homeostasis. Bronchial epithelial cells can also produce TGF-β either constitutively or upon stimulation such as by cigarette smoke [15, 16], whereas IFN-γ inhibits epithelial TGF-β release whilst promoting pIgR/SC expression. Intriguingly, it was shown that TGF-β expression is increased in small airway epithelium from smokers and patients with COPD [17, 18], and was suggested as a factor downregulating SLPI expression in these patients . A reduction in SLPI secretion can also be induced by elastase, as recently reported . Of note, no production of SLPI (or elafin) was detected in our study using Calu-3 cells (data not shown). Thus, it remains to investigate whether relationships between TGF-β signalling and epithelial functions—including production of pIgR/SC and SLPI—are affected in COPD.
In healthy subjects, a balance between oxidants and antioxidants keeps the extracellular environment in a reduced state. Cigarette smoke, which contains massive amounts of oxidants, is the most important risk factor for the development of COPD . The major nonenzymatic antioxidant of the lung is glutathione (GSH) . Our results showed that glutathione or N-acetylcysteine, a glutathione precursor, inhibited the epithelial SC production induced by TGF-β or by neutrophils. TGF-β is produced in an inactive form, complexed to the latency-associated peptide (LAP), and this complex needs to be dissociated to release active TGF-β. Reactive oxygen species (ROS) are able to oxidize LAP and to release active TGF-β, this effect being prevented by oxidant scavenging . The main cellular sources of ROS in the lung not only include activated phagocytes such as neutrophils but also structural cells such as endothelial cells, alveolar and bronchial epithelial cells [23, 24]. Accordingly, it is plausible that in our study GSH and NAC prevented TGF-β activation by neutrophil (or epithelial cell)—derived oxidants, thereby inhibiting TGF-β—induced SC production. In addition, antioxidants could also affect epithelial intracellular signalling, including p38 MAPK activation [25, 26]. Similarly, the natural antiproteinase SLPI might modulate TGF-β- and neutrophil-driven epithelial cell activation, such as NFκB pathway . However, our data support that SLPI further promotes SC upregulation upon neutrophil coculture not by affecting epithelial cell signalling but rather by inhibiting elastase activity.
Intracellular kinases play a crucial role in the control of activation of (anti)inflammatory genes in the airways, in T-cell function and tissue remodelling. In particular, MAP kinases control in airway epithelial cells the transcription of several genes involved in COPD pathogenesis such as mucins and inflammatory mediators (e.g., TNF-α, IL-8, and matrix metalloproteinases) . Neutrophil- and TGF-β-driven SC upregulation in epithelial cells was almost completely abolished by the inhibitor SB203580, indicating the requirement for p38 MAPK activation, in contrast to ERK and JNK pathways. In human bronchial epithelial cells, exposure to oxidants (peroxide) activates MAPK cascades, particularly stress responsive p38 MAPK [25, our data]. Thus, oxidative stress, caused by inflammatory cells and/or by cigarette smoke derivatives may upregulate epithelial pIgR/SC production by activating p38 MAPK. Increased numbers of p38 positive cells were previously observed in alveolar walls of patients with COPD, as compared to smokers and nonsmoking controls . Moreover, p38 inhibitors are effective in several animal models of inflammatory disorders, including COPD . For instance, the inhibitor SB2439063 reduced neutrophilia and expression of inflammatory mediators in a rat model of COPD . However, it has been observed that cytokine-induced synthesis of elafin, another natural epithelial-derived neutrophil elastase inhibitor, is also dependent on p38 MAPK [32, 33]. Thus, activation of p38 MAPK pathway might be critical in the airways to both inflammatory responses and transactivation of protective epithelial genes, including local anti-proteinases and pIgR/SC.
In conclusion, this paper demonstrates that activated neutrophils trigger increased epithelial pIgR/SC expression through redox- and p38 MAPK-dependent mechanisms. It also highlights a novel mucosal function for TGF-β, upregulating not only IgA synthesis but also its receptor-mediated transepithelial transport, and further suggests that the redox balance plays an important role in modulating epithelial responses to neutrophils. Thus, this paper provides a molecular basis for the dual effect of neutrophils on mucosal/secretory immunity which consists of on the one hand on proteolytic cleavage of pIgR/SC (and IgA) by elastase and proteinase-3 , and on the other hand on TGF-β-mediated activation of pIgR/SC synthesis via oxidant- and p38-dependent pathways. These findings are relevant to airway cell biology and neutrophilic airway diseases, suggesting that imbalance between the two identified opposite regulatory mechanisms of pIgR/SC may lead to downregulation of secretory IgA immunity, as observed in smokers who developed severe COPD . The cellular and molecular determinants of this (im)balance and fine tuning of pIgR expression will be further investigated in primary cultures of airway epithelial cells from COPD patients.
This paper was part of a grant from the Fonds National de la Recherche Scientifique (FNRS Belgium, grant F.R.S.M. 3.4582.08). C. Ratajczak is a recipient of a Grant from the Fonds Spéciaux de la Recherche (FSR 2008), Université catholique de Louvain (UCL) and C. Pilette is a recipient of a clinicien-chercheur fellowship from the FNRS.