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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Virology. Author manuscript; available in PMC 2011 September 15.
Published in final edited form as:
Virology. 2010 September 15; 405(1): 139–148.
Published online 2010 July 1. doi: 10.1016/j.virol.2010.05.031

Figure 3

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SARS-CoV S and SARS-CoV SPN have similar half-lives

At 24 h post- transfection, HEK293T cells expressing SARS-CoV S (solid line) or SPN (dashed line) were labeled with 35S-methionine/cysteine for 20 min and chased for 0, 1, 2 or 3 h. After lysis, S protein was immunoprecipitated, separated by SDS-PAGE and analyzed by autoradiography. Percentage of S or SPN remaining at each time point was calculated using the amount of S or SPN at 0 h chase as 100%. The average of 3 independent experiments ± SEM is shown.

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