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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Virology. Author manuscript; available in PMC 2011 September 15.
Published in final edited form as:
Virology. 2010 September 15; 405(1): 139–148.
Published online 2010 July 1. doi: 10.1016/j.virol.2010.05.031

Figure 2

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SARS-CoV S lacking all cytoplasmic cysteines is not palmitoylated

(A) Cytoplasmic tail sequence of SARS-CoV S and a mutant lacking all 9 cytoplasmic cysteines (SARS-CoV SPN). (B) At 24 h post-transfection, HEK293T cells expressing SARS-CoV S or SPN were labeled with 35S-methionine/cysteine or 3H-palmitic acid for 30 min. After lysis, S protein was immunoprecipitated, separated by SDS-PAGE and analyzed by fluorography. Longer exposure (6 wks) of the 3H-palmitate labeled samples confirmed the absence of palmitoylated SARS-CoV SPN.

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