SARS-CoV S lacking all cytoplasmic cysteines is not palmitoylated
(A) Cytoplasmic tail sequence of SARS-CoV S and a mutant lacking all 9 cytoplasmic cysteines (SARS-CoV SPN). (B) At 24 h post-transfection, HEK293T cells expressing SARS-CoV S or SPN were labeled with 35S-methionine/cysteine or 3H-palmitic acid for 30 min. After lysis, S protein was immunoprecipitated, separated by SDS-PAGE and analyzed by fluorography. Longer exposure (6 wks) of the 3H-palmitate labeled samples confirmed the absence of palmitoylated SARS-CoV SPN.