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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Biochem Biophys Res Commun. Author manuscript; available in PMC 2010 August 2.
Published in final edited form as:
PMCID: PMC2913709
NIHMSID: NIHMS210527

Vanadate-based transition-state analog inhibitors of Cre–LoxP recombination

Abstract

Cre recombinase exchanges DNA strands at the LoxP recognition site via transphosphorylation reactions that involve pentacoordinate transition states. We demonstrate that meta-vanadate ion (VO3) and appropriate DNA substrates assemble a transition-state analog-like complex in the Cre active site. Meta-vanadate inhibits recombination of LoxP-derived oligonucleotide substrates that contain a gap at either or both scissile phosphates, but does not inhibit reactions with intact LoxP. The 3′-hydroxyl group of the gapped substrate is required for inhibition, suggesting that vanadate is ligated by three oxo ligands. Assembly of the inhibited complex is slow (t1/2 = 19 min at 4 mM NaVO3) and requires Cre, substrates, and meta-vanadate. Holliday junction intermediates accumulated at lower meta-vanadate concentrations, suggesting that the second strand exchange is inhibited more readily than the first. The apparent KD for meta-vanadate is 1.5–2 mM and binding shows positive cooperativity. This methodology may have general application for mechanistic studies of recombinase/topoisomerase-mediated strand exchange reactions.

Keywords: Integrase family, Tyrosine recombinase, Type 1B topoisomerase, Site-specific recombination, Half-sites recombination, Cre recombinase, Meta-vanadate transition-state analogs, DNA cleavage and religation, Pentacoordinate transition state, Phosphotransfer reaction

Bacteriophage P1 Cre protein catalyzes recombination by promoting a double-strand cleavage and religation of DNA at the 34 bp LoxP sequence [1,2]. Cre-mediated recombination is a fundamental tool for bioengineering because chromosomal rearrangements can be controllably induced in living cells in vitro and in vivo [3]. Cre belongs to the Integrase (Int) family of tyrosine recombinases and Type 1B topoisomerases, which includes FLP recombinase and human and Vaccinia virus Topoisomerase I. Int proteins share a common active site structure and chemical mechanism for reversible DNA cleavage [4,5], in which a tyrosine nucleophile attacks the “scissile” phosphate, displacing the 5′-hydroxyl group of the S1′ nucleotide and forming a covalent protein–DNA intermediate (Fig. 1A). In topoisomerases, the strand is resealed after one supercoil is relaxed when the 5′-hydroxyl generated by the cleavage event displaces the tyrosine. In recombinases, strand exchange takes place when the 5′-hydroxyl generated in a neighboring active site displaces the tyrosine, creating a Holliday junction intermediate (Fig. 1B). A subsequent conformational change induces an analogous cleavage and religation on the second strand, resolving the Holliday junction and leading to the crossover products [6,7]. One function of the Int active site is to stabilize geometry and charge distribution of high energy pentacoordinate phosphoryl transition states. In Cre–LoxP crystal structures, Arg173, Arg292, and Trp315 seem to be positioned for this role. However, recent biochemical studies of Vaccinia virus Topoisomerase 1B have suggested alternate roles for these residues. His289 (Cre numbering), previously proposed to act as a general base for Tyr324 activation, is instead proposed to stabilize the transition state along with Arg292, while Arg173and Lys201 are proposed to act as part of a proton shuttle [8,9]. To perform these functions, the pKas must be highly perturbed, and precisely how the Int active site promotes strand exchange remains somewhat controversial.

Fig. 1
(A) General mechanism of transesterification reactions catalyzed by Int tyrosine recombinase/Type 1B topoisomerase family. The nucleotides on the 5′ and 3′ sites of the cleavage phosphate are denoted “S1” and “S1′.” ...

A direct method to identify protein ligands involved in phosphoryl transfer or hydrolysis transition-state stabilization is through crystallographic visualization of complexes containing oxo-vanadate inhibitors in enzyme active sites [10-19]. Vanadates replace the phosphate group and readily achieve trigonal-bipyramidal geometry through axial coordination by substrate and/or protein hydroxyl groups [20-22] (Fig. 1C). In crystal structures of such complexes, the amino acid side chains that stabilize the transition state negative charge and pentacoordinate geometry interact with the vanadate equatorial oxo groups via hydrogen bonds and salt bridges. For phosphatases, generally ortho-vanadate (vanadium(VI), VO42) and the product alcohol assemble the active site inhibitor with the axial attacking water being mimicked by a vanadate oxygen (structure 1 in Fig. 1C) [11,13,15,16,22]. In phosphoryl-transfer reactions, meta-vanadate (vanadium(V), VO3) becomes sandwiched between the leaving and attacking hydroxyl groups (structure 2 in Fig. 1C) [10,12,14]. More recently, both ortho- and meta-vanadates have been demonstrated to assemble trivalent complexes containing a third equatorial ligand [17,19]. Particularly relevant to Int family strand exchange reactions, Rupert et al. [17] crystallized a meta-vanadate transition-state complex of the hairpin ribozyme catalytic RNA, which cleaves RNA by promoting an internal transphosphorylation. In the X-ray structure, the attacking 2′- and leaving 5′-hydroxyls coordinated the vanadium at axial positions and the remaining 3′-hydroxyl coordinated an equatorial position (structure 3 in Fig. 1C). By analogy, we reasoned Cre might assemble similar trivalent complexes with meta-vanadate (structure 4 in Fig. 1C).

Methods and materials

Homogeneous Cre recombinase was purified as previously described [23]. RPC-purified oligonucleotide substrates were obtained from DNA Express, quantified by UV absorbance, and mixed in 10 mM Tris–Cl, 1 mM EDTA, pH 8.0, to yield the specified duplexes. The substrate plasmid containing a single LoxP site, pLT38(+)–LoxP [24], was purified using a Qiagen Midiprep Purification Kit. BanI/StuI fragments were prepared and 32P 5′-labeled with polynucleotide kinase as previously described [24]. The substrate LoxP-3′dd was prepared by separately incubating 6 μM of each half-arm of LoxP-ΔS1 with 60 μM ddGTP or ddATP and 125 U/ml DNA Pol I Klenow fragment (NEB) in the supplied buffer for 40 min at 21 °C, followed by a treatment at 70 °C for 10 min, and then combining the two reactions to make a 3 μM stock. All recombination reactions were carried out using optimized Cre reaction buffer [23] at 21 °C. For non-preincubation experiments, the reactions were initiated by mixing equal volumes of twofold concentrated solutions of Cre recombinase and oligonucleotide substrates and with twofold concentrated reaction buffer and plasmid or labeled substrate. For preincubation experiments, Cre and oligonucleotides, with or without NaVO3, were mixed in 10 mM Tris–Cl, 1 mM EDTA, and 30 mM NaCl, allowed to stand on ice for the indicated times, and then rapidly mixed with a twofold concentrated stock of reaction buffer containing the same vanadate concentrations and the indicator substrate. Reactions were terminated after 30 min with 1% SDS, 0.3mM EDTA, 5 mM DTT, 6% glycerol, and 0.025% bromophenol blue. Radiolabeled reactions were additionally digested with 1 mg/ml proteinase K in 20 mM EDTA. Electrophoresis was carried out using either 1% agarose/TAE buffer for plasmid, or 8–10% Lamelli SDS polyacrylamide gels [25]. DNA was visualized using either ethidium bromide or autoradiography [26]. Reactions with labeled substrate typically yield 30–40% turnover, 80–90% products, and 10–20% intermediate.

Results and discussion

LoxP consists of two inverted 13bp repeats, which recruit one Cre molecule each [2]. Two Cre2 LoxP dimers then associate to form the active tetrameric recombination synapse [7]. DNA cleavage occurs within the “8 bp spacer” segment between the two 13bp repeats (Fig. 2A) [27]. Vanadate complexes are generally labile in solution and are stabilized in active sites by favorable contacts with residues that interact with the reaction transition-state [10,20]. At least two hydroxyl groups must be present to supply axial ligands for the meta-vanadate anion, VO3 (Fig. 1C, 2 and 3). LoxP derivatives were constructed (Fig. 2B) in which the scissile phosphate was removed from both strands (LoxP-cl) or each strand individually (LoxP-hcl1 and -hcl2), leaving free 3′- and 5′-hydroxyl groups to act as potential meta-vanadate ligands. Although these gapped molecules cannot form covalent intermediates with Cre, they can resolve covalent LoxP-derived intermediates because of the free S1′ 5′-hydroxyl groups (Fig. 2C, lanes 4, 6, and 8). This property allows these DNAs to be used both as substrates in so-called “half-site recombination” [28] and as potential inhibitors when complexed with vanadate.

Fig. 2
(A) 34 bp LoxP site. The 13 bp inverted repeat Cre binding sequences are boxed. Cleavage occurs within the 8 bp spacer between the S1 and S1′ bases (black vertical arrows). (B) Sequences of the 8 bp spacer regions of gapped LoxP derivatives used ...

After preincubation with 5 mM sodium meta-vanadate (NaVO3), reactions with the gapped substrates are markedly inhibited (Fig. 2C, lanes 5, 7, and 9, and Fig. 2D, lane 3), while the reaction with LoxP, in which the scissile phosphate would be expected to block the assembly of the meta-vanadate complex, is essentially unaffected (Fig. 2C, lanes 2 and 3). At high concentrations of NaVO3, greater than 10 mM, some inhibition (10–20%) is observed in the LoxP reactions, but this contribution is negligible at the concentrations used here (data not shown). To assemble an inhibited complex, a free 3′-hydroxyl from the S1 base must also be present. LoxP-ΔS1 is a LoxP derivative that lacks the S1 scissile base (Fig. 2B) and is inactive as a substrate (Fig. 2D, lanes 4 and 5). LoxP-ΔS1 was extended to yield a LoxP-cl derivative, LoxP-3′dd (Fig. 2B), that contained the S1 nucleotide but lacked scissile 3′-hydroxyl groups. While LoxP-3′dd acted as a substrate because of the free 5′-hydroxyl (Fig. 2D, lane 6), the reaction was not affected by NaVO3 (Fig. 2D, lane 7).

The level of inhibition is concentration-dependent between 0 and 10 mM (Fig. 2E, left panel) with maximal inhibition occurring at greater than 5 mM. In addition, the efficacy of inhibition is enhanced by preincubation of Cre, LoxP-cl, and NaVO3 prior to adding the indicator substrate (Fig. 2E, right panel). To quantitate these effects on the overall reaction and on each of the cleavage steps, inhibition experiments were performed using a 200 bp radiolabeled LoxP-containing restriction fragment as an indicator substrate (Fig. 3A) [24,26]. With this methodology, the disappearance of substrate, the level of Holliday junction intermediate (the product of the first strand exchange), and the level of recombination products (the product of the second strand exchange) could be simultaneously monitored. Under these conditions, the formation of recombination products is inhibited to nearly 90% by NaVO3 at 10 mM, with a concentration of half-maximal inhibition (C50) of ~2 mM when no preincubation is employed (Fig. 3B, open circles). With a 90 min preincubation, lower concentrations of NaVO3 are required for half-maximal and maximal levels of inhibition, 1.5 and ~6 mM, respectively (Fig. 3B, filled squares). The shapes of the inhibition curves are also dramatically different. The curvature of the non-preincubation curves cannot be fit to a simple binding isotherm. More homogeneous inhibition is observed with 90 min preincubation and the inhibition curve can be modeled as a single cooperative binding process (Fig. 3D). Interestingly, the accumulation of products and Holliday junction intermediate is differentially sensitive to NaVO3 concentration (Fig. 3C, compare open triangles and filled squares). At low NaVO3 concentrations Holliday junction formation is enhanced, becoming maximal at 2 mM, before also becoming inhibited. Plotting the inhibition binding curves and fitting them in a modified Hill equation [26] yielded apparent dissociation constants and Hill numbers, respectively, of 1.9 mM and 2.2 for turnover of substrate, and 1.5 mM and 2.6, for the production of products. These results suggest that the second strand exchange is somewhat more sensitive to meta-vanadate inhibition than the first.

Fig. 3
Quantitation of dose–response and effect of preincubation. (A) Sample SDS–PAGE analysis of the effects of increasing NaVO3 concentrations (0–10 mM) on “half-sites recombination” reactions with no preincubation (See ...

Cre, the Lox substrate, and NaVO3 are required to observe the preincubation effect (Fig. 4A). Reactions in which two of the three components were preincubated gave similar levels of recombination product as reactions with no preincubation, suggesting that the rate-limiting step for the formation of inhibited species involves requires simultaneous interactions between the Cre active site, meta-vanadate, and the substrate hydroxyls. The time dependence of inhibition at 4 mM NaVO3 was also investigated (Fig. 4B). The majority of the inhibited complex assembled in a first-order process with a half-time of nearly 20 min (Fig. 4C).

Fig. 4
(A) Requirements for preincubation effect. Various combinations of Cre (2400 nM), LoxP-cl (600 nM), and 5 mM NaVO3, as depicted below the panel, were preincubated for 90 min, diluted twofold and into twofold concentrated buffer and labeled substrate, ...

The slow onset of inhibition, and its dependence on meta-vanadate and the appropriate DNA substrate, is consistent with the idea that a transition-state complex is assembled. Indeed, slow onset of inhibition is a signature of transition-state analogs [29,30]. In the transition state of phosphotransfer reactions, the attacking and leaving groups, in this case the tyrosine and deoxyribose 5′ hydroxyl groups, are colinear with the phosphate and occupy the axial positions. In the Cre–Lox complex, the requirement for the S1 base 3′-hydroxyl suggests several possible geometries. The most likely geometry, in direct analogy to the hairpin ribozyme/meta-vanadate complex [17], is that the attacking Tyr324 and leaving S1′ 5′-hydroxyl groups occupy the axial positions, and the S1 3′-hydroxyl occupies one of the equatorial positions (Fig. 1C, 4). Alternatively, meta-vanadate could form a bridge between the 3′-hydroxyl group and either the S1′ 5′-hydroxyl or Tyr324. However, to achieve the required colinear geometry, the 3′-Tyr or 3′–5′ bridges would require a substantial shift in either the DNA or Tyr324, and it is not readily apparent how the active site could productively interact with this arrangement. A second geometric issue is the source of the 5′-hydroxyl ligand, which could originate from a non-bridging or bridging strand, that is from the first cleavage or final resealing events, or creation or resolution of the Holliday junction, respectively (Fig. 1B). The proclivity for Cre to form Holliday junction complexes with DNA [24] suggests that the 5′-hydroxyl likely originates from the bridging strand.

A crystal structure of this complex is expected to reveal the Cre active-site ligands responsible for ligating the vanadate oxo groups, and in turn for stabilizing the transition state of the authentic recombination reaction. Meta-vanadate may also prove useful for studying the mechanisms of other Integrase family proteins, in which the role of active site ligands is still in question.

Acknowledgments

This work was supported from Section of Molecular and Cellular Biology startup funds at UC Davis, NIH grant R01-GM063109, and an American Cancer Society Institutional Research Grant (No. 36769) from the UC Davis Cancer Center.

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