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Logo of jcinvestThe Journal of Clinical InvestigationCurrent IssueArchiveSubscriptionAbout the Journal
J Clin Invest. 2010 August 2; 120(8): 2684–2698.
Published online 2010 July 26. doi:  10.1172/JCI42118
PMCID: PMC2912191

Sema3E–Plexin D1 signaling drives human cancer cell invasiveness and metastatic spreading in mice


Semaphorin 3E (Sema3E) is a secreted molecule implicated in axonal path finding and inhibition of developmental and postischemic angiogenesis. Sema3E is also highly expressed in metastatic cancer cells, but its mechanistic role in tumor progression was not understood. Here we show that expression of Sema3E and its receptor Plexin D1 correlates with the metastatic progression of human tumors. Consistent with the clinical data, knocking down endogenous expression of either Sema3E or Plexin D1 in human metastatic carcinoma cells hampered their metastatic potential when injected into mice, while tumor growth was not markedly affected. Conversely, overexpression of exogenous Sema3E in cancer cells increased their invasiveness, transendothelial migration, and metastatic spreading, although it inhibited tumor vessel formation, resulting in reduced tumor growth in mice. The proinvasive and metastatic activity of Sema3E in tumor cells was dependent on transactivation of the Plexin D1–associated ErbB2/Neu oncogenic kinase. In sum, Sema3E–Plexin D1 signaling in cancer cells is crucially implicated in their metastatic behavior and may therefore be a promising target for strategies aimed at blocking tumor metastasis.


Semaphorins are secreted and membrane-bound signals, originally implicated in the control of axonal wiring (1). They comprise a wide protein family and are involved in a range of functions, from tissue morphogenesis to the immune response, to cancer progression. High-affinity semaphorin receptors are found in the families of the plexins and of the neuropilins (2). The relevance of the small cytoplasmic domain of neuropilins in semaphorin signaling is unclear; however, the intracellular domain of plexins has been characterized for controlling R-Ras and RhoA activity and hence negatively regulates integrin-mediated adhesion and cell migration (1, 3). In addition, other molecules can interact with semaphorins or semaphorin receptors on the cell surface, featuring a complex scenario of multiple potential signaling pathways. In particular, tyrosine kinase receptors may become activated in response to semaphorins (47). This often leads to an apparent biological paradox whenever a semaphorin, in addition to mediating inhibitory signals (e.g., cell repulsion, inhibition of cell migration, etc.), can furthermore promote cell migration and invasive growth by activating tyrosine kinases in a cell-specific manner (8).

The role of semaphorin signalling in tumor progression is currently under close scrutiny. It is known that cancer cells release semaphorins regulating their own behavior as well as that of cells in the tumor microenvironment, such as endothelial cells and recruited leukocytes (see refs. 9 and 10 for reviews). For example, semaphorin 3B (Sema3B), Sema3F, and Sema4D have been shown to differently regulate tumor growth, tumor angiogenesis, and metastatic progression (1116). Moreover, based on scattered evidence, semaphorin expression levels might discriminate among tumor cells with different metastatic abilities (13, 17, 18). In this work, we focused on Sema3E (previously known as semaH), which was found to be overexpressed in metastatic tumor cells (17). We have previously demonstrated that, like other class 3 semaphorins, Sema3E is synthesized as a full-length precursor molecule of approximately 87 kDa (p87-Sema3E), which is then subjected to proteolytic maturation by furin proprotein-convertases (PPCs), yielding a smaller fragment of approximately 61 kDa, referred to as p61. We have further shown that this proteolytic fragment promotes lung colonization of tumor cells injected in the circulation (19), but the implicated mechanisms remained unclear.

The high-affinity functional receptor of Sema3E is Plexin D1 and not any of the neuropilins (20). Genetic evidence showed that Sema3E–Plexin D1 signaling is required in developmental angiogenesis (20, 21). Moreover, recent evidence indicates that Sema3E is implicated in regulating postischemic angiogenesis (22). Intriguingly, while Plexin D1 expression is generally low in normal adult tissues, it is elevated in endothelial cells of tumor vessels and in cancer cells (23, 24). Like other family members, Plexin D1 is able to trigger R-Ras inactivation, leading to axonal and cell repulsion in vitro (25). Moreover, it was shown that Sema3E mediates either axonal attraction or repulsion in distinct neuronal populations, depending on the coexpression of Neuropilin-1 with Plexin D1 (26). This suggests that Sema3E can mediate divergent functions in different cells, depending on the implicated receptor complexes. Intriguingly, according to a recent report, Sema3E could inhibit the survival of MDA-MB-435 tumor cells in vitro but not their growth in vivo, while the opposite was true for MDA-MB-231 carcinoma cells (27); however, the mechanisms involved could not be elucidated. In the present work, we show that Sema3E is a double-faced signal in cancer development, eliciting 2 mirror effects in tumor cells and in the endothelial cells of tumor vessels, and further elucidate the implicated signaling pathways. Moreover, we show that Sema3E expression in human primary tumors is statistically associated with their metastatic progression and demonstrate in mouse preclinical models that interfering with Sema3E signaling in primary tumors results in a striking reduction of their metastatic dissemination.


Sema3E expression correlates with the metastatic potential of human tumors.

Sema3E was initially identified in mouse metastatic cancer cells (17). However, whether the expression of Sema3E or its receptor Plexin D1 in human primary tumors may be correlated with the metastatic progression has not been assessed. We analyzed gene expression in a wide series of 60 human colon carcinoma samples, for which we obtained detailed clinicopathological information (including the possible development of metastasis), as well as in a separate series of excised liver metastases (Supplemental Figure 1A; supplemental material available online with this article; doi: 10.1172/JCI42118DS1). While the expression of the receptor Plexin D1 was clearly detected in all primary tumors, Sema3E levels were above detection threshold in only a fraction of them (independently from tumor grade or stages of local invasiveness; data not shown). Interestingly, Sema3E was expressed in 88% of the cases associated with metastatic disease (M1) and in only 46% of the nonmetastatic tumors (M0). This biased distribution was statistically significant, as confirmed by Fisher’s exact test (P = 0.004). Consistently, as shown in Table Table1,1, average Sema3E expression levels were significantly higher in metastatic versus nonmetastatic primary tumors (P = 0.002). Moreover, we could invariably detect Sema3E expression in 100% of liver metastases from colon carcinomas, and average levels of Sema3E and Plexin D1 were significantly higher in metastasis compared with primary tumors. Thus, Sema3E–Plexin D1 signaling seems to correlate positively with the metastatic progression of human colon carcinomas.

Table 1
Relative gene expression levels in human colorectal tumors and metastases

Interestingly, a recent immunohistochemistry analysis showed that Plexin D1 receptor levels are increased in invasive and metastatic melanomas, compared with low-grade tumors and benign nevi (28). Previous data on Sema3E expression in melanomas were controversial; thus, we analyzed a wide series of benign nevi, low-grade melanomas, and highly invasive and metastatic melanomas, by using a polyclonal antibody previously validated for detecting all isoforms of Sema3E, including the proteolytically activated fragment p61 (19). We found that Sema3E was expressed in only a fraction of the melanocytes forming benign or dysplastic nevi as well as of the melanoma cells of low grade; in contrast, the percentage of Sema3E-positive cells increased to almost 100% in invasive and metastatic melanomas of Clark levels III and IV (Figure (Figure1).1). Moreover, Sema3E expression at the single cell level seemed to be higher in malignant melanomas, compared with nevi. Thus, according to our data, also in melanoma progression, Sema3E signaling in primary tumors correlates with the potential for metastatic spreading.

Figure 1
Elevated Sema3E expression in invasive human melanomas.

Knocking down either Plexin D1 or Sema3E expression in cancer cells curtails metastatic spreading.

We have demonstrated above that Sema3E levels directly correlate with the invasive and metastatic phenotype of human tumors of different histotype. We therefore investigated whether the genetic knockdown of either Sema3E (or its receptor Plexin D1) in validated experimental tumor models could affect the metastatic behavior. As shown in Figure Figure2A,2A, we first silenced Plexin D1 expression in human metastatic carcinoma cells A549 (expressing endogenous Sema3E) and transplanted them in athymic mice to study tumor development and metastatic dissemination. Interestingly, Plexin D1–deficient tumors developed as controls (Figure (Figure2B),2B), suggesting that Plexin D1 expression in cancer cells do not have a major role in regulating growth or survival. In contrast, the number of lung metastases that spontaneously arose from Plexin D1–depleted primary tumors was significantly reduced (Figure (Figure2C),2C), indicating a pivotal role of this receptor in the invasive-metastatic process.

Figure 2
Plexin D1 and Sema3E knockdown in tumor cells inhibits the metastatic spreading.

In order to assess the functional relevance of endogenous Sema3E expression in tumor cells, we furthermore generated and transplanted in vivo Sema3E-deficient A549 cells (Figure (Figure2,2, D–F). Analogously, we knocked down Sema3E expression in highly metastatic 4T1 breast carcinoma cells and carried out tumor transplantation experiments in syngenic mice, both subcutaneously and in the orthotopic site of mammary fat pad (Figure (Figure2,2, G–L). Although the growth of the subcutaneous and orthotopic tumors was not significantly affected upon Sema3E silencing (Figure (Figure2,2, H and J), their spontaneous metastatic dissemination was significantly reduced (Figure (Figure2,2, I and K). Moreover, knocking down the endogenous Sema3E expression greatly reduced the metastatic potential of tumor cells injected in the systemic circulation (Figure (Figure2L).2L). These data were further validated using a second independent shRNA targeting the Sema3E transcript (Supplemental Figure 2, A and B). In addition, and consistent with our expression data in human tumor samples, we found that the metastatic potential of HCT-116 colon carcinoma cells was significantly reduced upon Sema3E knockdown (Supplemental Figure 2C). Altogether, our results indicate that the expression of both Sema3E and Plexin D1 is crucially involved in the metastatic spreading of diverse cancer cells.

Sema3E overexpression inhibits tumor vessel density and tumor growth but promotes metastatic spreading.

Prompted by the results reported above, we questioned whether a gain of Sema3E expression could in turn enhance the metastatic potential of tumor cells. Three human cancer cell lines (i.e., HCT-116 colon carcinoma, MDA-MB-435 melanoma, and A549 lung carcinoma) were therefore transduced with lentiviral vectors encoding Sema3E or empty vector (EV) controls. Overexpressing cells contained a small amount of the full-length Sema3E precursor of approximately 87 kDa (p87-Sema3E) as well as a predominant amount of the matured fragment p61 (Supplemental Figure 3A and data not shown), indicating an efficient spontaneous proteolytic conversion. Notably, p61 was also the main species of endogenous Sema3E released by cancer cells (including those used for our gene knockdown experiments; see Supplemental Figure 3B). Moreover, we found that the p61 fragment was fully competent to bind the receptor Plexin D1 and elicit the typical collapsing response (Supplemental Figure 3, C and D). For these reasons, in many subsequent experiments, a recombinant form of the matured p61 fragment was also used.

Sema3E overexpression did not elicit morphological changes in cancer cells (data not shown), and we did not observe any significant difference in viability and proliferation in culture (Supplemental Figure 4A). Sema3E-expressing and control cells were then transplanted subcutaneously in nude mice to establish primary tumors. Remarkably, the elevated expression of Sema3E led to a significant reduction in tumor volume and weight in both models (Figure (Figure3A3A and Supplemental Figure 4B). By assessing the mitotic index (with Ki-67; Figure Figure3B)3B) in tumor tissue sections, we found that Sema3E expression did not significantly affect tumor cell proliferation, consistent with in vitro analysis. However, cellular apoptosis in Sema3E-expressing tumors was increased by approximately 2 fold, as revealed by TUNEL (Figure (Figure3C)3C) and further confirmed by the detection of the apoptotic markers cleaved caspase-9 and lamin B (Figure (Figure3D).3D). Notably, we found that the vascular density of Sema3E-expressing tumors was significantly reduced (Figure (Figure3E);3E); moreover, Sema3E had significantly induced endothelial cell apoptosis (Figure (Figure3F).3F). These data suggested a potential paracrine activity in the microenvironment of the semaphorin released by tumor cells.

Figure 3
Sema3E overexpression inhibits tumor vessel density and tumor growth.

When looking at the metastatic spreading from these primary tumors, we found that — despite their reduced size — tumors overexpressing Sema3E gave rise to a strikingly increased number of spontaneous lung metastases (Figure (Figure4A)4A) expressing Sema3E (Supplemental Figure 5A), which indicated a predominant effect of the semaphorin to enhance the invasive-metastatic process. Notably, tumor xenografts overexpressing full-length processable Sema3E mainly contained mature p61 fragments (Supplemental Figure 5B), further confirming in vivo an efficient proteolytic conversion of the precursor. Moreover, the expression of a recombinant p61 fragment in tumors resulted in comparable effects in vivo, as observed with wild-type processable Sema3E undergoing spontaneous proteolytic conversion (Supplemental Figure 5, C–E, and Figure Figure4B). 4B).

Figure 4
Sema3E-p61 signaling in cancer cells enhances the metastatic spreading.

To establish the functional relevance in vivo of Sema3E signaling in cancer cells, as opposed to its potential paracrine activity on endothelial cells, we knocked down the expression of the receptor Plexin D1 in MDA-MB-435 cells. Plexin D1–silenced cancer cells were viable and did not display any morphological change in culture (data not shown). Importantly, we found that Plexin D1 knockdown in cancer cells did not prevent the inhibitory effect of p61-Sema3E on tumor growth (Figure (Figure4C),4C), tumor vessel density (Figure (Figure4D),4D), or endothelial cell apoptosis (data not shown), supporting the idea that secreted Sema3E acts on the tumor endothelium in a paracrine manner. In contrast, p61-Sema3E overexpression could not induce the metastatic behavior of these Plexin D1–deficient cells (Figure (Figure4E).4E). Thus, the prometastatic activity of Sema3E is a tumor cell–autonomous, Plexin D1–dependent effect, independent from the paracrine activity capable of endothelial cells repulsion. These results could explain the apparent paradox observed in our experimental tumors in vivo and suggested 2 independent functions mediated by p61-Sema3E in different cell types, via the same receptor, Plexin D1.

Overall, forced overexpression of Sema3E in cancer cells upholds a paracrine antiangiogenic effect and leads to reduced tumor growth, a functional effect which was not observed with endogenous expression levels. On the other hand, both gene knockdown and overexpression experiments indicated that the Sema3E-dependent prometastatic effect depends on a distinctive signalling cascade into tumor cells, mediated by the Plexin D1 receptor.

Sema3E-dependent endothelial cell repulsion relies on Rnd2-expression and R-Ras inactivation.

In an attempt to unravel the mechanisms underlying the effects of Sema3E on tumor vessels and metastasis, we performed in vitro experiments analyzing endothelial and tumor cells, respectively.

Functional assays in culture demonstrated that Sema3E (and its matured fragment p61) was capable of inhibiting the migration of HUVECs in a Plexin D1–dependent manner and eliciting their repulsion and cellular collapse (Figure (Figure5,5, A and B, and Supplemental Figure 6, A and B). Moreover, in a mixed-culture experiment reminiscent of the in vivo setting, we found that Sema3E-expressing tumor cells could efficiently induce endothelial cell repulsion only if the latter contained Plexin D1 (Supplemental Figure 6B).

Figure 5
Molecular mechanisms of Sema3E-dependent endothelial cell repulsion.

It was recently shown that cell collapse and axonal repulsion mediated by Plexin D1 depend on its intrinsic R-Ras GAP activity (25). To investigate the relevance of this pathway in endothelial cells treated with Sema3E, we overexpressed a dominant-active mutant of R-Ras that cannot be regulated by Plexin D1 (25) and found that endothelial cells were no longer sensitive to p61-dependent inhibition of migration (Figure (Figure5C).5C). Moreover, it was reported that the GAP activity of Plexin D1 depends on the association of the small GTPase Rnd2 (25). Notably, we found that Rnd2 is abundantly expressed in HUVECs (Supplemental Figure 6C); moreover, upon knocking down Rnd2 expression, these cells became insensitive to Sema3E-mediated inhibition of migration (Figure (Figure5D)5D) and cellular collapse (Figure (Figure5E).5E). These data indicate that the inhibitory function of Sema3E in endothelial cells is dependent on the R-Ras GAP activity of Plexin D1 and on their prominent expression of Rnd2. Altogether, these data are consistent with the reported restrictive role of Sema3E in developmental and postischemic angiogenesis (20, 22) and with our in vivo data indicating inhibition of tumor vasculature.

p61-Sema3E signaling in tumor cells promotes motility and invasiveness, transendothelial migration, and extravasation in vivo.

We aimed at elucidating the molecular mechanisms responsible for the prometastatic activity of p61-Sema3E. As demonstrated above in Figure Figure4E,4E, our experimental evidence indicates that this function crucially depends on signaling cascades deployed into the tumor cells, which could potentially regulate their intrinsic migratory and invasive/metastatic behavior. In keeping with this hypothesis, we found that different cancer cells were induced to migrate in response to p61-Sema3E, either provided in the environment (Figure (Figure6A)6A) or constitutively overexpressed in autocrine manner (Figure (Figure6B);6B); moreover, this function was dependent on endogenous Plexin D1 receptor expression (Figure (Figure6B).6B). Notably, unlike HUVECs, none of the cancer cells analyzed underwent the collapsing response upon Sema3E treatment (Supplemental Figure 6A and data not shown), possibly reflecting their relatively low expression of the required mediator Rnd2 (see Supplemental Figure 6C). Moreover, knocking down Rnd2 expression did not impair Sema3E-induced cancer cell migration (Supplemental Figure 10A). Thus, cancer cells and endothelial cells respond differently to Sema3E/Plexin D1 signaling.

Figure 6
p61-Sema3E induces tumor cell migration and invasiveness.

Further elucidating the mechanisms involved in the prometastatic activity of p61-Sema3E in tumor cells, we found that it promotes the invasion of the ECM (Figure (Figure6C).6C). Moreover, p61 expression induced ECM degradation spots, colocalizing with actin-enriched focal structures (Supplemental Figure 7), resembling adhesive contacts called invadopodia, which are known to mediate the localized release of ECM-degrading enzymes (29). Notably, ECM degradation and invadopodia are instrumental for cancer cells slithering in and out blood vessel walls in order to metastasize. To address the potential involvement of p61-Sema3E in tumor cell extravasation, we initially exploited a transendothelial migration assay in vitro. We found that p61-expressing tumor cells, unlike controls, promptly migrated through a compact monolayer of endothelial cells (Figure (Figure6D).6D). Importantly, p61-induced transendothelial migration was abrogated in cancer cells lacking Plexin D1 receptor (Figure (Figure6D). 6D).

It is known that a limited fraction of the cells released in the circulation by primary tumors forms metastases. Some of these cells die soon after entering the blood stream; others get entrapped in narrow capillary beds (e.g., in the lungs) and die in thrombotic emboli or manage to return in the circulation. A minority of circulating cancer cells will manage to extravasate, eventually forming metastatic colonies. In order to elucidate in vivo the role of Sema3E in tumor cell extravasation, we injected intravenously fluorescence-labeled MDA-MB-435 cells expressing p61 (or EV-controls) and subsequently sacrificed mice at different time points (from 15 minutes to 48 hours after injection) to quantify metastatic spreading. Initially, as expected, tumor cells accumulated in the lungs, independent from their expression of p61-Sema3E (Figure (Figure7A)7A) and consistent with the mechanical entrapment in the first encountered capillary bed. As expected, the number of cancer cells present in the lungs gradually decreased over time. However, by 1 hour after injection, p61-Sema3E–expressing cells were found in strikingly greater number compared with controls. At 48 hours after injection, 10-fold more p61-expressing cells were present in the lungs. This is consistent with the fact that p61- expressing cells directly injected in the circulation gave origin to a larger number of metastatic lung colonies, as scored in long term experiments (Supplemental Figure 8A). Moreover, similar experiments were performed by using Plexin D1–silenced cancer cells. In this case, p61-Sema3E could not induce the metastatic behavior (Figure (Figure7B),7B), thereby confirming that the proinvasive and prometastatic activity displayed by p61-Sema3E is dependent on a signaling cascade intrinsic to tumor cells.

Figure 7
p61-Sema3E enhances cancer cell metastatic extravasation.

Furthermore, we used a drug-inducible system to control semaphorin expression in tumor cells injected in vivo (see Supplemental Methods for details). Two groups of mice received cells in which p61-Sema3E expression had been induced before injection; for one group drug treatment was maintained until the end of the experiment, whereas for the other group, treatment was stopped 4 days after injection (in order to terminate p61 expression after extravasation in lungs had occurred). Other mice received untreated cells, and p61 expression began only 4 days after injection. One month later, the lungs were analyzed to score the presence of metastases. We found that p61-Sema3E expression in cancer cells, at the time of injection, resulted in a 10-fold increase in the number of metastatic colonies compared with that of controls (even if drug-induced expression was terminated soon after). On the contrary, the induction of semaphorin expression after the extravasation step only produced a negligible increase in the number of detectable lung colonies compared with that of controls (Supplemental Figure 8B). These data strongly suggested that p61-Sema3E mainly acts by promoting tumor cell extravasation.

The prometastatic activity of p61-Sema3E is mediated by the transactivation of Plexin D1–associated ErbB2.

Other semaphorins, including Sema4D and Sema6D, have been shown to elicit cancer cell migration and invasive growth (46). Interestingly, the implicated receptors (i.e., Plexin B1 and Plexin A1) interact with receptor tyrosine kinases (RTKs) and trigger their distinctive signal pathways and functional outcomes. By analogy, p61-Sema3E could elicit the activation of a RTK associated with Plexin D1, putatively responsible for the observed prometastatic effect. We therefore undertook an unbiased screening approach to search for potential candidates in a low-density array, designed to simultaneously detect the tyrosine phosphorylation of 42 different RTKs in cell lysates. Interestingly, we found that ErbB2 phosphorylation was selectively increased in A549 carcinoma cells in response to p61-Sema3E (Supplemental Figure 9). ErbB2 is an oncogenic receptor often implicated in cancer invasion and metastasis; it has no cognate ligand but commonly becomes activated by forming heterodimers with other receptors of the same family (such as EGFR or ErbB3) (3032). We have therefore individually analyzed immunopurified ErbB2 (Figure (Figure8A)8A) and the homologous ErbB3 and EGFR tyrosine kinases (Figure (Figure8B)8B) in cells treated with p61-Sema3E. In addition to that of ErbB2, we observed a minor increase in EGFR tyrosine phosphorylation induced by p61, while ErbB3 was not affected. ErbB2 phosphorylation in response to p61 was further confirmed in HeLa (Figure (Figure8C)8C) and MDA-MB-435 cells (data not shown). Notably, while p61 cannot bind ErbB2 (data not shown), it elicited ErbB2 phosphorylation in a Plexin D1–dependent manner, as demonstrated by receptor knockdown (Figure (Figure8C). 8C).

Figure 8
p61-Sema3E elicits the activation of Plexin D1–associated tyrosine kinase ErbB2.

Interestingly, we could copurify Plexin D1 and ErbB2 from lysates of overexpressing cells (Figure (Figure8D),8D), suggesting that they can form receptor complexes on the cell surface. Moreover, Plexin D1 became tyrosine phosphorylated in this complex upon p61 stimulation (Figure (Figure8E),8E), and this was impaired by an ErbB2 kinase inhibitor but not upon inhibition of the unrelated RTK Met (Figure (Figure8E).8E). Finally, and most importantly, we found that the 2 endogenous receptors Plexin D1 and ErbB2 associated in a complex in tumor cells, upon stimulation with p61-Sema3E (Figure (Figure8F). 8F).

We then found that p61-induced migration of cancer cells was impaired upon inhibition of ErbB2 catalytic activity (Figure (Figure9A9A and Supplemental Figure 11, A and B). Moreover, p61-expressing cells were unable to give rise to lung metastatic colonies upon systemic treatment of recipient mice with ErbB2 inhibitor (Figure (Figure9B).9B). Furthermore, by knocking down ErbB2 expression in cancer cells with RNAi technology, we could selectively impair p61-Sema3E signaling, both in vitro and in vivo. In fact, while ErbB2-depleted cells were viable and sensitive to motogenic signals, like hepatocyte growth factor, they did not respond to p61 in migration assays (Figure (Figure9C)9C) or in experimental metastasis assays in vivo (Figure (Figure9D).9D). Taken together, these data indicated that ErbB2 plays a master role in the invasive-metastatic properties orchestrated by p61-Sema3E and its receptor.

Figure 9
ErbB2 kinase is required for p61-dependent promigratory and prometastatic function.

ErbB2-mediated signaling is well characterized, and MAPK, PI3K-AKT, and PLC-γ appear to be the main players (30, 31). Interestingly, MAPK and PLC-γ cascades are typically activated in response to EGFR-ErbB2 heterodimers, while the PI3K-AKT pathway is the main mediator of ErbB2-ErbB3 complexes. We therefore analyzed the individual contribution of these signal transducers in Sema3E-induced tumor cell migration by applying the selective inhibitors LY294002, PD98059, and U-73122. As shown in Figure Figure9E,9E, MAPK and PLC-γ activation appeared to be required for function, while the PI3K-AKT pathway was only marginally implicated. Moreover, our analysis of MAPK, AKT, and PLC-γ phosphorylation in tumor cells treated with Sema3E indicated a major activation of MAPK and PLC-γ (Supplemental Figure 12). Thus, our data suggest that Sema3E/Plexin D1–dependent activation of a signaling cascade downstream to ErbB2 phosphorylation, especially via MAPK and PLC-γ activation, is mainly responsible for the observed proinvasive and prometastatic function.


Semaphorins and their receptors have been implicated in a range of functions, including angiogenesis and tumor progression (9, 10). In particular, the functional relevance of Sema3E in cancer was poorly understood, and the implicated signaling mechanisms had not been elucidated. Notably, a common feature of guidance cues, including Sema3E, is their ability to act as double-faced signals. Here we found that Sema3E is able to independently regulate endothelial and tumor cells, triggering divergent functional outcomes, through the involvement of distinctive signaling pathways. When we asked about the functional relevance of Sema3E signaling in tumor development and metastatic progression, we found that Sema3E endogenously expressed in tumor cells is a major determinant of their invasive-metastatic behavior in vivo. Moreover, and relevant to the clinics, we found that Sema3E expression in human primary tumors correlates with their potential to form metastasis.

Sema3E had been initially identified in cancer cells; however, it was unclear whether its expression could impinge on tumor growth. We found that Sema3E overexpression reduced tumor burden in vivo. According to our data, this was not accounted for by direct suppression of tumor cell growth. In fact, the ectopic expression of Sema3E in different cancer cells did not inhibit their proliferation or survival in culture, including upon anchorage-independent growth in soft agar (A. Casazza and L. Tamagnone, unpublished observations). Moreover, while apoptosis was increased in Sema3E-overexpressing tumors, the proliferation index of cancer cells was not significantly reduced. In fact, the tumor suppressor effect observed in our experiments was likely accounted for by the reduced vascular density induced by sustained paracrine signaling of ectopic Sema3E released in the tumor microenvironment, consistent with the endothelial repelling function observed in developmental angiogenesis (20). Moreover, we elucidated that the endothelial-repelling and inhibitory activity of Sema3E depends on the R-Ras GAP activity of Plexin D1 and on the expression of the regulatory molecule Rnd2. It is noteworthy that the potential inhibitory activity of endogenous Sema3E levels released by tumor cells is probably overpowered in vivo by proangiogenic factors in the tumor microenvironment. Consistent with this hypothesis, we found that interfering with endogenous Sema3E expression in cancer cells did not increase tumor growth, while it significantly curbed metastatic spreading.

We have reported previously that Sema3E undergoes proteolytic cleavage by furin-like PPCs. This maturation process is strictly required to unleash the prometastatic activity of the semaphorin, since a mutated uncleavable form of full-length Sema3E has no prometastatic activity (19). In fact, PPCs are commonly expressed in tumor cell lines and human cancers, and our present data indicate that the small proteolytic fragment p61 is the main form of Sema3E found in the conditioned media of tumor cells and in experimental tumor samples. Importantly, we have shown here that the p61 fragment is fully competent to bind the functional receptor Plexin D1, and it carries an endothelial cell–repelling activity comparable with that of the full-length molecule.

In apparent contrast with its potential inhibitory function in endothelial cells, p61-Sema3E enhances the inherent invasiveness of tumor cells and their metastatic behavior. Moreover, the receptor Plexin D1 is responsible for this autocrine effect, since knocking down its expression in tumor cells abates their metastatic potential and abrogates any proinvasive and metastatic response to p61. Metastasis formation is a multistep process, starting with tumor cell invasiveness at the primary tumor site and implicating cell migration, cell survival, and matrix degradation as well as the ability to overcome tissue barriers, such as vessel walls, and ultimately leading to colonization of distant tissues (33). In our study, we found that p61-Sema3E promotes tumor cell migration and invasiveness, possibly mediated by invadopodia formation. Notably, on the basis of multiple functional experiments in vitro (including adhesion-independent growth and survival in soft agar), we have ruled out that p61 may exert an antiapoptotic activity in tumor cells, potentially impinging on their survival in the blood stream (A. Casazza and L. Tamagnone, unpublished observations). Instead, we found that the Sema3E expression levels dictate the ability of circulating cancer cells to extravasate and form metastatic colonies.

A few other semaphorins have been found to promote cell migration by transactivating plexin-associated tyrosine kinase receptors in a cell-specific manner (46). This suggested that an RTK could be similarly involved in Sema3E signaling, especially since we had observed Plexin D1 phosphorylation on tyrosine upon p61 stimulation. Primed by the results of a phospho-proteomic screening, we then demonstrated that ErbB2 is the tyrosine kinase receptor responsible for p61-mediated signaling, leading to the invasive-metastatic response. Notably, both the treatment with an ErbB2 inhibitor and the selective knockdown of this RTK by RNAi resulted in a dramatic reduction of p61-induced tumor cell migration and metastatic lung colonization.

ErbB2 tyrosine kinase receptor is commonly expressed in human tumors, but it has no cognate ligand, and it mainly becomes activated by transphosphorylation in association with the homologous receptors EGFR or ErbB3 (3032). We observed a minor increase of EGFR but not ErbB3 phosphorylation in tumor cells treated with p61-Sema3E, possibly suggesting that Plexin D1 signalling impinges on the activation of ErbB2-EGFR receptor heterodimers found in cancer cells but not in endothelial cells (see our preliminary data in Supplemental Figure 13, A and B). Moreover, MAPK and PLC-γ appeared to be the main signal transducers involved in the pathway, while PI3K-AKT activation was only marginally implicated. This is consistent with that reported in literature about the predominant involvement of PLC-γ and MAPK pathways downstream to ErbB2-EGFR complexes (30, 31).

It has been shown in neurons that neuropilin-1 expression levels can dictate differential functional responses to Sema3E in different cell populations (26). Intriguingly, neuropilin-1 is expressed in HUVECs at an intermediate level between those found in the 2 tumor cell models used in our functional experiments in vivo, i.e., MDA-MB-435 and A549 cells (data not shown). Moreover, p61-Sema3E cannot interact with neuropilin-1 (data not shown). Thus, our data do not support the idea that neuropilin expression alone may account for the functional differences between Sema3E activity in endothelial and tumor cells. Interestingly, we found that the small GTPase Rnd2, associated with Plexin D1, is required for the inhibitory activity of Sema3E in endothelial cells while it is expressed at low levels and not implicated in Sema3E function in tumor cells (see Supplemental Figure 10, A and B). Conversely, tumor cells are characterized by the expression of ErbB2-EGFR heterodimers, which are involved in Sema3E-induced invasion and metastasis.

Previously published data on the expression of Sema3E and its receptor in human tumors do not univocally establish a correlation with tumor progression. For instance, we have shown high Sema3E levels in mammary carcinomas (19). Moreover, others have found an elevated expression in high- versus low-grade glioblastomas (34), while the opposite was true for bladder carcinomas (35). As expected, the functional relevance of Sema3E as a master regulator of the invasive-metastatic phenotype may depend on the activity of additional tumor type–specific regulatory pathways as well as on individual genetic variability. Of note, Roodink and coworkers, in an immunohistochemistry analysis of nevi and melanoma samples, came to the puzzling conclusion that while Plexin D1 expression is positively correlated with tumor metastatic progression, Sema3E levels are regulated in the opposite manner (28). We have now analyzed a wide panel of nevi and melanoma samples, using a validated antibody proven to detect all isoforms of Sema3E, including p61 (19). Our data demonstrate a significant increase of Sema3E expression in highly invasive and metastatic melanomas, compared with that of benign nevi and noninvasive melanomas. Notably, our data are further consistent with those seen in 2 independent gene expression data sets (36, 37) and support the postulated role of Sema3E signaling in the invasive-metastatic progression of melanomas.

We have furthermore analyzed the expression of Sema3E and its receptor in a wide series of colon cancer samples, for which we obtained detailed clinicopathological information. We found that while Sema3E expression is often below the detection threshold in nonmetastatic tumors, it is almost invariably present in primary tumors, giving rise to distant metastasis. Moreover, the average expression levels of both Sema3E and Plexin D1 were significantly increased in excised metastases compared with those of primary tumors, suggesting the acquisition of a selective advantage for metastatic spreading in cell-autonomous manner. This was confirmed upon analyzing gene expression in a subset of 8 primary tumors with available matched metastatic tissue from the same patients. In fact, the levels of both Plexin D1 and Sema3E were further increased in the metastasis compared with the primary tumor in 100% and 75% of these cases, respectively (Supplemental Figure 1B). Moreover, the median expression value had a more than 6-fold increase in metastases for both genes, and this difference passed statistical significance tests for Plexin D1 (P = 0.004).

Considering our data on the involvement of ErbB2 in p61-Sema3E signaling, one would expect that metastatic human tumors expressing Sema3E and Plexin D1 should also contain ErbB2. We clearly detected ErbB2 expression in all primary colon carcinoma samples included in our panel (average of transcripts per 104 copies β-actin, 15.32 ± 2.57 ± SEM). However, there was no significant statistical correlation between ErbB2 expression levels and the metastatic or nonmetastatic behavior of these tumors (data not shown). This indicates that, not unexpectedly, the ErbB2 oncogene is not the driving force for the metastatic spreading of colon cancers, but it is widely expressed in tumors, consistent with an effector function in Sema3E–Plexin D1 signaling cascade.

We finally asked whether Sema3E expression could have any relevance as a predictor of clinical outcome in human cancers. Although providing a conclusive answer to this question is beyond the scope of this study, we looked carefully into our data on colon cancer patients. As expected, while early stage tumors (i.e., T1-T2) did not associate with metastasis, advanced stage colon cancers (i.e., T3-T4) had an overall 28% incidence of metastasis in our series (12 out of 43 cases). However, we noticed that among Sema3E-negative tumors the incidence of metastasis was only 10% (2 out of 19), whereas Sema3E-positive primary tumors had a 58% incidence of metastasis (14 out of 24). Although the size of this sample is small, Fisher’s exact test confirmed that this is not a random distribution (P = 0.03), suggesting that Sema3E expression may predict the risk of metastatic progression in advanced tumors. To further assess the clinical significance of Sema3E expression in primary colorectal tumors, we analyzed a subset of patients for which the clinical outcome after 2 years of follow-up was known (Supplemental Figure 1C). Interestingly, Kaplan-Meier analysis revealed that Sema3E levels in primary tumors inversely correlated significantly with patient survival. Indeed, the overall 2-year survival rate was 94% in patients with Sema3E-negative primary tumors but only 58% in patients with Sema3E-positive tumors (P = 0.02 by log-rank test; n = 16 for Sema3E-negative tumors and n = 12 for Sema3E-positive tumors). Thus, our data strongly suggest that Sema3E expression and signaling (via Plexin D1) correlate positively with metastatic progression and poor prognosis of human colon carcinomas. Moreover, taken together, our findings indicate Sema3E as an important “modifier” signal in cancer progression, potentially active in multiple human tumors.

In conclusion, we showed that Plexin D1 stimulation by p61-Sema3E can elicit 2 independent signaling cascades and divergent functional effects in cancer cells and in endothelial cells. Moreover, we elucidated the functional relevance in vivo of Sema3E and Plexin D1 endogenously expressed in tumor cells, by showing that their partial knockdown is sufficient to abate the metastatic potential, while we ruled out that blocking endogenous Sema3E might promote tumor growth. We have furthermore provided statistical evidence in colon cancer patients that an elevated expression of Sema3E (and Plexin D1) strikingly increases the risk of metastatic progression. In sum, Sema3E autocrine signaling is crucially implicated to induce a metastatic behavior in multiple cancer cells, and it appears as a promising target for strategies aimed at blocking tumor metastasis.


Further information can be found in the Supplemental Methods.

Antibodies and other reagents.

Myc-tagged and VSV-tagged proteins were detected by MoAb 9E10 (Santa Cruz Biotechnology Inc.) and P5D4 (Sigma-Aldrich), respectively. Other antibodies were as follows: anti-mouse CD31 (MEC 13.3, BD Biosciences), anti–Ki-67 (DAKO), anti-activated caspase-3 (Cell Signaling Technology), anti-endoglin (R&D Systems), anti-phosphotyrosine (4G10, Upstate), biotinylated anti-human Plexin D1 (aa 47–1271, R&D Systems), anti-ErbB2 (29D8, Cell Signaling Technology, and e2-4000, Invitrogen), anti-EGFR (LA22, Millipore), anti-ErbB3 (C-17, Santa Cruz Biotechnology Inc.), anti-Rnd2 (Proteintech Group Inc.), anti–Caspase-9 (96.1.23, Santa Cruz Biotechnology Inc.), anti-laminB (101-B7, Calbiochem), anti-actin (C-11, Santa Cruz Biotechnology Inc.), anti-HA (Y-11, Santa Cruz Biotechnology Inc.), and anti-Vinculin (V4505, Sigma-Aldrich). Antibodies to Sema3E were described previously (19). Recombinant human Heregulin-β1 Extracellular Domain (Hrg-β) and HGF were from R&D Systems. Human recombinant EGF was from Sigma-Aldrich. Lapatinib (GW572016, Tykerb) was provided by GlaxoSmithKline or purchased from Sellek Chemicals. Met inhibitor PHA665752 was from TOCRIS Bioscience. LY294002 and PD98059 were from Calbiochem, and U-73122 was from Sigma-Aldrich.

Ectopic gene expression in mammalian cells.

Expression constructs encoding full-size mouse Sema3E (fused with a polyHis-Myc-epitope tag at C’-term) as well as truncated p61-Sema3E (19) were subcloned into the lentiviral transfer plasmid pRRLsin.cPPT.hCMV.GFP.Wpre, and gene transfer was performed as previously described (38). This method ensured stable gene transfer with very high efficiency, without the need to select individual cell clones. In addition, we assayed multiple independent batches of cells transduced with each construct. The ErbB2 mammalian expression construct was a gift from Thomas Diettmar (Witten/Herdecke University, Witten, Germany). A dominant-active R-Ras was provided by Dominique T. Brandt (University of Heidelberg, Heidelberg, Germany). An expression construct for Rnd2 was purchased from Missouri S&T cDNA Resource Center.

Purification of Sema3E molecules.

COS cells were transduced to stably express the respective Sema3E molecules. Conditioned media were harvested from these cells in the absence of serum. Protein purification was achieved using a Nickel-loaded affinity column (GE-Pharmacia) according to the manufacturer’s protocols.

Knockdown of gene expression by RNAi.

The expression of Plexin D1, Sema3E, ErbB2, and Met was silenced in tumor and/or endothelial cells by expressing gene-targeted shRNA. Rnd2 expression was knocked down in HUVECs by transfection with targeted siRNA duplexes. Details on reagents, procedures, and the targeted sequences are provided in the Supplemental Methods.

Real-time quantitative PCR analysis of gene expression.

Total RNA from tumor cell lines or tissues was isolated by using RNeasy Protect Mini Kit (Qiagen) according to the manufacturer’s instructions. cDNA preparation was performed according to standard procedures, using M-MLV Reverse Transcriptase (Promega) and oligo-dT primers (Sigma-Aldrich). Real-time quantitative PCR (Q-PCR) analysis was performed using the Applied Biosystems 7900HT Fast Real-Time PCR System and PCR primers and TaqMan probes listed in Supplemental Methods. Gene expression in cells subjected to RNAi-mediated knockdown was quantified as relative levels versus mock-transduced cells. On the other hand, gene expression in individual human tumor samples was quantified as relative number of transcripts compared with β-actin. Informed consent was received for human samples. Care and use of human samples were approved by review boards at the University of Heidelberg (Germany), and the Institute for Cancer Research and Treatment (Candiolo, Turin, Italy).

MTT cell viability assay.

Transduced tumor cells were seeded in multiple 24-well plates (5 × 104 cells/well). After 24 hours of serum starvation, the cells were cultured in DMEM medium containing 0.1% FBS. To reveal viability, the cells were incubated with 500 μl/ml of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) dye solution (Sigma-Aldrich) for 4 hours at 37°C. The indicator product, formazan, was resuspended in DMSO and measured spectrophotometrically at 570 nm by a Titertek Multiskan reader.

Fluorescence labeling of living cells.

MDA-MB-435 cells were incubated with 10 μM CFDA SE Vybrant Cell Tracer (Molecular Probes) for 15 minutes at 37°C. Then the loading solution was replaced with fresh, prewarmed medium and the cultures were incubated for another 30 minutes at 37°C.

Cell migration and invasion assays.

Cell motility was assayed using Transwell chamber inserts (Costar), with a porous polycarbonate membrane (8-μM pore size) as described previously (39). To allow haptotactic migration, the lower side of the filter was coated with 10 μg/ml fibronectin. Cells were harvested from culture dishes by treatment with 1 mM EDTA and resuspended in 0.2% BSA-containing medium. Approximately 1 × 105 cells were added in the upper chamber and allowed to migrate through the filter toward the lower chamber (including regulatory molecules, when indicated) for 6–8 hours in a cell culture incubator. Then, the cells adherent to the upper side of the filter were mechanically removed, while those that migrated to the lower side of the filter were fixed with 11% glutaraldehyde and stained with crystal violet. The dye was then solubilized in 10% acetic acid to measure absorbance at 595 nm in a microplate reader (OD values are shown in the graphs as arbitrary units). For ECM invasion assays, 5 × 105 tumor cells were added to the upper chamber of a Transwell insert coated with Matrigel (20 μg/well; Becton Dickinson) and allowed to migrate in 1% FBS-containing medium. After 16 hours of incubation, the cells adherent to the lower side of the porous membrane were fixed and analyzed as above.

Transendothelial migration of tumor cells.

Approximately 1 × 105 HUVECs were added to type I collagen-coated 24-well Transwell inserts with small pore sizes (5-μm pore size; Costar) and grown to confluence for 3 days, with daily replacement of fresh culture medium, until they formed a tightly packed monolayer. Then, 7 × 105 CFDA-SE–labeled (see above) MDA-MB-435 cells, resuspended in HUVEC culture medium, were added to the upper chamber. After incubation for 16 hours, the cells were fixed with 11% glutaraldehyde, and the upper side of the membranes were gently scraped with a cotton swab to remove nonmigrated cells. Transmigrated tumor cells were quantified based on green fluorescence in 5 random fields, using METAMORPH software.

Animal model and in vivo procedures.

In vivo studies were conducted in 6- to 8-week-old immunocompromised Cd1–/– nude athymic or BALB/C female mice (Charles River Laboratory). Unless otherwise indicated, in each experiment, we analyzed 6 tumors per each condition. For subcutaneous tumor models, 2 × 106 MDA-MB-435, 8 × 106 A549, or 5 × 105 4T1 cells were injected into the right posterior flank of anesthetized animals. Tumor size was measured externally every 2 days using a caliper, and tumor volume was estimated using the equation V = 4/3p × (d/2)2 × D/2, where d indicates the minor tumor axis, D indicates the major tumor axis, and V indicates volume. Mice were sacrificed after 30–60 days after transplant, and tumors were weighted after dissection. Superficial pulmonary metastases were contrasted using black India ink to infuse airways, and counted on dissected lung lobes under a stereoscopic microscope. For orthotopic injection, 1 × 106 4T1 cells resuspended in 40 μl phosphate-buffered saline were injected into the second mammary fat pad of anesthetized Balb/c mice, following exposure by a small incision. Mice were sacrificed after 25 days, and tumors and lungs were measured as mentioned above. For experimental metastasis assays, 2 × 106 MDA-MB-435, 6 × 106 A549, or 2 × 105 4T1 cells were injected into the lateral mouse tail vein; for long-term experiments, cell colonies were scored in the lung using black India ink to infuse airways, as described above. Short-term metastatic extravasation of fluorescent cancer cells in the lungs was quantified by analyzing 4 pairs of lungs for each experimental group (at least 10 independent microscopic fields each) with METAMORPH software. For experiments based on in vivo Lapatinib treatments, mice received the vehicle (0.5% hydroxypropylmethylcellulose with 0.1% Tween 80 in water) or the drug (100 mg/kg body weight) daily by oral gavage, from 3 days before to 2 days after systemic tumor cell injection. Animal procedures had been approved by the Ethical Commission of the University of Torino and by the Italian Ministry of Health.

Histological analysis.

Paraffin-embedded or OCT-embedded frozen samples were cut in 10-μm thick sections and probed with primary antibodies (listed above). Streptavidin was conjugated with HRP (DAKO), while all secondary antibodies used were conjugated with Alexa Fluor 488 or Alexa Fluor 546 fluorochromes (Molecular Probes). To detect apoptotic cells in tumor sections, we used an indirect TUNEL assay, using the ApopTag Green In Situ Apoptosis Detection Kit (Chemicon). Slides were analyzed using a Leica DM IRBM microscope, and digital images were evaluated with METAMORPH software. Unless otherwise indicated, quantification was done by analyzing at least 3 sections and 10 fields per tumor (considering 6 tumors per experimental condition).

Immunoprecipitation, SDS-PAGE, and Western immunoblotting.

Cellular proteins were analyzed using standard methods, as described in the Supplemental Methods, using antibodies listed above. Band intensity quantification was done with Quantity-One software.


Statistical significance was verified using the 2-tailed Student’s t test. P values of less than 0.05 were considered significant.

Supplementary Material

Supplemental data:


We gratefully acknowledge the help of E. Vigna, S. Corso, and S. Giordano (IRCC, Candiolo) and T. Diettmar (Witten/Herdecke University, Germany) and D.T. Brandt (University of Heidelberg, Germany) for generously sharing expression constructs important for this study. We thank E. Urbano, L. Palmas, and F. Sassi for skillful technical assistance; M. Galluzzo, F. Maina, T. Werdiningsih, and L. Tarditi for support with experiments in mice; F. Galimi for help with Q-PCR analysis; and S. Giordano, G. Neufeld, and C. Isella for useful discussion and suggestions. The work was supported by grants from Italian Association for Cancer Research and from Regione Piemonte (to L. Tamagnone, L. Trusolino, and P.M. Comoglio). A. Casazza is supported by a FIRC fellowship.


Conflict of interest: The authors have declared that no conflict of interests exists.

Citation for this article: J Clin Invest. 2010;120(8):2684–2698. doi:10.1172/JCI42118.

Charlotte Rolny’s present address is: University of Uppsala, Department of Genetics and Pathology, Vascular Biology Unit, Rudbeck Laboratory, Uppsala, Sweden.

See the related Commentary beginning on page 2658.


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