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Studies in mice and humans have revealed that the T cell, immunoglobulin, mucin (TIM) genes are associated with several atopic diseases. TIM-1 is a type I membrane protein that is expressed on T cells upon stimulation and has been shown to modulate their activation. In addition to a recently described interaction with dendritic cells, TIM-1 has also been identified as a phosphatidylserine recognition molecule, and several protein ligands have been proposed. Our understanding of its activity is complicated by the possibility that TIM-1 possesses multiple and diverse binding partners. In order to delineate the function of TIM-1, we generated monoclonal antibodies directed to a cleft formed within the IgV domain of TIM-1. We have shown here that antibodies that bind to this defined cleft antagonize TIM-1 binding to specific ligands and cells. Notably, these antibodies exhibited therapeutic activity in a humanized SCID model of experimental asthma, ameliorating inflammation, and airway hyperresponsiveness. Further experiments demonstrated that the effects of the TIM-1–specific antibodies were mediated via suppression of Th2 cell proliferation and cytokine production. These results demonstrate that modulation of the TIM-1 pathway can critically influence activated T cells in a humanized disease model, suggesting that TIM-1 antagonists may provide potent therapeutic benefit in asthma and other immune-mediated disorders.
Allergic asthma, which can be a chronic and debilitating disease, is characterized by leukocyte infiltration into the lung, Th2 cytokine responses (classically IL-4, IL-5, and IL-13), elevated levels of allergen-specific IgE, mucus secretion, and airway hyperresponsiveness (AHR) (1, 2). The rising prevalence of this disease and the persistent problem of unmet medical need for severe asthmatics has stimulated intensive research in asthma genetics (3). T cell, immunoglobulin, mucin receptor molecule 1 (TIM-1), originally identified as hepatitis A virus cellular receptor 1 (HAVCR1, also known as KIM1), a kidney injury response gene in rats and humans (4) and the African green monkey (5), has also been identified as an important susceptibility gene for human asthma (3, 6). Accumulating data in the murine system support the role of TIM-1 in Th2-dependent inflammation. The TIM gene family has been associated with Th2 cytokine expression and AHR (7), and anti-mouse TIM-1 mAbs reduce Th2 cytokine secretion and disease pathology in models of lung inflammation, allergic conjunctivitis, and allergic gut inflammation (8–11). However, in vivo data in the human system are lacking, and further experimental elaboration is essential to evaluate the clinical relevance of the TIM-1 pathway.
TIM proteins are type I membrane proteins with the extracellular region consisting of an IgV domain situated on top of a mucin-rich domain and a short membrane-proximal stalk containing N-linked glycosylation sites (4). Murine TIM-1, TIM-2, TIM-3, and TIM-4 IgV domains show a conserved, disulfide-dependent conformation in which the CC′ loop is folded onto the GFC β strands, forming a unique structure. In all TIM family members the CC′ and FG strand/loop configuration (CC′/FG) creates a unique, variably sized cleft as identified in crystallography studies (12, 13). Anti–TIM-1 mAbs can be derived that are directed to this unique CC′/FG cleft or to distinct epitopes within the TIM-1 extracellular region.
In this study, we characterize the biochemical properties of anti-mouse TIM-1 and anti-human TIM-1 mAbs. To assess their activity in human allergic inflammation, we utilize the SCID mouse model. SCID mice have a defective DNA recombinase system, are therefore deficient in mature and functional T and B lymphocytes, and fail to reject allogeneic and xenogeneic tissue transplants (14–17). SCID mice transplanted with human PBMCs (hu-PBMC SCID mice) have been successfully used to study immune responses. Mice reconstituted with PBMCs from asthmatic patients develop allergic disease characterized by human Th2 cytokine secretion, allergen-specific human IgE production, lung inflammation, and AHR (18–23). Using the hu-PBMC SCID model, we demonstrate here that anti-human TIM1 mAb treatment reduces the characteristic symptoms of the asthmatic response. These data support the genetic hypothesis that TIM-1 is associated with human asthmatic disease and suggest that anti–TIM-1 mAb treatment might represent a novel therapy for human asthma. Furthermore, we characterize the biochemical properties of therapeutic anti-mouse TIM-1 and anti-human TIM-1 mAbs and develop a model of TIM-1 mechanism of action based on the epitopes and activities of specific mAbs. These studies are the first to demonstrate that antagonism of human TIM-1 activity reduces pathologic immune responses in a human disease model.
Previously we described a panel of rat anti-mouse TIM-1 mAbs (isotype IgG2a) and showed that treatment with the anti-murine TIM-1 mAb 4A2 reduced lung inflammation in a mouse model (9). Using protease protection assays and Western blot analysis, we determined that anti-murine TIM-1 mAb 4A2 binds to a nonlinear epitope lying between the F strand and the C terminus of the IgV domain of mouse TIM-1 (Table (Table11 and our unpublished observation). Table Table11 summarizes the binding characteristics of anti-mouse TIM-1 mAb 4A2, compared with several other anti-mouse TIM-1 mAbs shown to bind to distinct domains of the mouse TIM-1 protein (9). Next we developed a panel of mouse anti-human TIM-1 mAbs (isotype IgG1). To identify anti-human TIM-1 mAbs with similar binding properties for human TIM-1, we made expression constructs in which the F and G strands and intervening loop (F > G) within the TIM-1 IgV domain were replaced with a murine sequence. This sequence swap encompasses one side of the cleft identified as important for mediating binding to phosphatidylserine (PS) (13, 24, 25). The top of this cleft contains a sialic acid–like binding site (12, 26). We transiently expressed these constructs in 293 cells and analyzed anti-human TIM-1 mAb binding by FACS. The binding activity of anti-human TIM-1 mAb A6G2 was disrupted by the F > G human/mouse sequence substitution (Table (Table2).2). Since this mAb recognized a nonlinear epitope (i.e., failed to bind reduced TIM-1 protein in Western blots; data not shown), it is likely that the epitope for anti-human TIM-1 mAb A6G2 is created by the disulfide-bound, 3-dimensional CC′/FG structure. Anti-human TIM-1 mAb P6A4 had a similar profile (Table (Table2).2). In contrast, other anti-human TIM-1 mAbs, shown in cross-blocking analyses to bind to distinct epitopes within the wild-type human TIM-1 IgV domain (our unpublished observation), were capable of binding to the mutated TIM-1 protein, suggesting that the overall structure of the mutant protein was not grossly affected by the sequence exchange (Table (Table2).2). The affinity of various anti-human TIM-1 mAbs for full-length wild-type human TIM-1–Fc was determined using an ELISA format. Relative affinities were very similar among the group of mAbs used in this analysis and in the following experiments (Figure (Figure1A1A and Table Table2). 2).
We next determined whether mAbs raised against murine and human TIM-1 proteins were capable of interfering with PS binding. First, using ELISA assays with Tris buffer lacking excess Ca++ or Mg++, PS binding curves for murine TIM-1 and human TIM-1 proteins were determined (Supplemental Figure 1; supplemental material available online with this article; doi: 10.1172/JCI39543DS1). Relative EC50 values of 0.78 μg/ml and 0.01 μg/ml were obtained for human and murine TIM-1 binding, respectively. We analyzed PS binding using the human TIM-1–Fc protein in Tris buffer containing 1 mM Ca++ and 1 mM Mg++, which produced an 11-fold increase in the relative affinity, to 0.07 μg/ml. Thus the relative affinity of TIM-1 for PS binding in the ELISA assay was affected by cation levels, as expected (13).
We used the Tris buffer containing 1 mM Ca++ and 1 mM Mg++ in a competition ELISA format with 3 μg/ml TIM-1–Fc fusion proteins and 10 μg/ml anti–TIM-1 mAbs to assess the functional activity of various anti-mouse TIM-1 mAbs and anti-human TIM-1 mAbs. Anti–TIM-1 mAbs had variable effects on PS binding. The anti-mouse TIM-1 mAb 4A2 and the anti-human TIM-1 mAb A6G2 strongly inhibited binding of PS to the murine and human fusion proteins, respectively (Figure (Figure1,1, B and C).
TIM-1 possesses both cation-dependent and cation-independent binding activity on mouse DCs (26). To characterize TIM-1 binding to DCs, we incubated mouse TIM-1–Fc or mouse TIM-1–IgV–Fc protein with murine bone marrow–derived myeloid DCs in the presence or absence of excess chelating agents and anti-mouse TIM-1 mAbs, and performed FACS analyses. Both the murine TIM-1–Fc and TIM-1–IgV–Fc proteins bound to DCs (Figure (Figure2,2, A and B). EGTA (1 mM) (or 1 mM EDTA; data not shown) reduced binding of murine TIM-1–Fc to DCs (Figure (Figure2A),2A), as previously described (26). Importantly, the binding of murine TIM-1 to these DCs required only the IgV domain (Figure (Figure2B).2B). To further demonstrate the specificity of TIM-1–Fc binding to DCs, we used a variety of blocking reagents including 5% human sera, 20 μg/ml heat-aggregated human IgG, or 10% rabbit sera. None of these reagents had a significant impact on binding (Supplemental Figure 2 and data not shown). Furthermore, we used irrelevant human receptor–IgG1 Fc fusion proteins at the same concentration as the human TIM-1–IgG1 Fc (3 μg/ml) to stain CD11c+ DCs in human PBMC preparations. Neither CD40-Fc nor LTβR-Fc bound to DCs in this assay (Supplemental Figure 2), as would be expected.
Anti-mouse TIM-1 mAb 4A2 reduced binding of murine TIM-1 proteins to DCs in the presence of 1 mM EGTA (Figure (Figure2A2A and Table Table3)3) to background level, similar to the unstained control (data not shown). Other anti-mouse TIM-1 mAbs or rat IgG2a isotype control mAb did not affect binding of the TIM-1 fusion proteins to murine myeloid DCs in the presence of 1 mM EGTA (Table (Table3).3). Similar results were obtained using CD11c+ mouse DCs freshly isolated from mouse blood (data not shown). LPS-activated murine myeloid DCs had increased levels of CD80, CD86, and MHC-II staining in FACS analyses (data not shown). Activation of these DCs increased the level of TIM-1–Fc binding (the MFI increased from 351 to 471), and this increase was blocked with EGTA and anti–TIM-1 mAb 4A2 similarly to nonactivated DCs (Figure (Figure2C).2C). These results demonstrate that TIM-1 binds to DCs even in the presence of excess chelating agents, as originally described by Murphy and colleagues (26), is dependent on the TIM-1–IgV domain, and is selectively ablated by an anti-mouse TIM-1 mAb that binds the IgV domain within the F > G region proposed as critical for multiple TIM-1 binding activities (13, 24, 25).
To determine whether anti-human TIM-1 mAb A6G2 had a similar impact on human TIM-1 interaction with human DCs, CD34+ stem cells were cultured to derive myeloid DCs, kept in culture or activated with LPS for 48 hours, then incubated with 5 μg/ml TIM-1–Fc protein in the presence or absence of 1–2 mM EGTA and 10 μg/ml anti-human TIM-1 mAbs or control mAb. Similar to the findings using the mouse system, anti-human TIM-1 mAb A6G2 was able to block TIM-1 protein binding to myeloid DCs in the presence of 1–2 mM EGTA (Figure (Figure2D2D and data not shown). The level of staining in the presence of both mAbs and EGTA was similar to the background staining observed with the unstained control (data not shown). Incubation of anti-human TIM-1 mAb A6G2 with human PBMC preparations blocked TIM-1–Fc binding on activated CD11c+ DCs, similar to the results obtained using anti-murine TIM-1 mAb 4A2 (Figure (Figure3A).3A). In contrast, TIM-1–Fc binding to other cell populations including CD3+ T cells and CD19+ B cells was negligible. This suggested that the robust binding seen in specific DC populations was unique to DCs and not solely associated with PS recognition.
To further test the hypothesis that mAb interaction with the F > G region was associated with antagonistic activity in the CD11c+ DC binding assays, we screened 27 additional anti-human TIM-1 mAbs raised against the IgV domain. Within this panel of mAbs, the only additional mAb capable of antagonizing human TIM-1–Fc binding to CD11c+ DCs was P6A4, another mAb shown to bind to the F > G region in the domain mutagenesis experiments (Table (Table22 and Figure Figure3B).3B). Thus, we found that in both the mouse and human systems, only those mAbs that interacted with the region of TIM-1 IgV domain containing the F > G strands and loop reduced TIM-1 binding to cultured myeloid DCs or to freshly obtained CD11c+ DCs. We then compared the pattern of binding of human TIM-1–Fc to DCs with that of Annexin-V, a canonical PS ligand, using FACS analysis. Freshly isolated CD11c+ DCs from human PBMCs bound Annexin-V at a low level in a manner that was very sensitive to the presence of chelating agent (1 mM EDTA), giving a specific MFI of 88. In contrast, human TIM-1–Fc bound DCs with a specific MFI of 144 even in the presence of 2 mM EGTA. Incubation with 1 mM EGTA or 1–2 mM EDTA gave similar results. In all cases TIM-1–Fc binding was reduced to background levels (MFI 18) by incubation with anti-human TIM-1 mAb A6G2. To compare the level of PS staining on freshly isolated DCs with that on apoptotic cells, DCs were treated with 1 μM staurosporin. Apoptotic DCs showed dramatically increased Annexin-V binding with an MFI of 4,162. These results suggest that TIM-1 binding to DCs in the presence of excess chelating agents may be mediated by a molecule other than PS. Importantly, the strikingly similar biochemical behavior of the anti-human TIM-1 mAb A6G2 to its murine counterpart 4A2 motivated testing its therapeutic efficacy in an in vivo system.
To determine whether anti-human TIM-1 mAb A6G2 could reduce the symptoms of experimental allergic asthma as does the anti-murine TIM-1 mAb 4A2 (9), a model using hu-PBMC SCID mice was established (Figure (Figure4).4). As described previously (22, 23), SCID mice were reconstituted i.p. with PBMCs from asthmatic donors allergic to the house dust mite Dermatophagoides pteronyssinus allergen. Following reconstitution, recipient mice received multiple i.p. sensitizations with D. pteronyssinus to maintain and boost the allergen-specific immune response and were subsequently challenged with aerosolized D. pteronyssinus to trigger the allergic asthmatic phenotype (Figure (Figure4).4). Using this protocol, a cohort was included to evaluate the effect of anti-human TIM-1 mAb A6G2 treatment (Table (Table4).4). Additional cohorts (Table (Table4)4) included an irrelevant isotype IgG control, an anti-human IL-13 mAb, and an anti-human TIM-1 mAb, A3H1, whose epitope lies in a region of the human TIM-1 IgV domain that is distinct from that recognized by anti-human TIM-1 mAb A6G2 (Table (Table11 and our unpublished observation). The neutralizing anti-human IL-13 mAb was included as a putative positive control (27). Negative control mice were reconstituted with PBMCs from nonallergic donors. All groups were subsequently challenged with aerosolized D. pteronyssinus.
Asthmatic hu-PBMC SCID mice had an increase in leukocyte numbers and human IL-4 levels (Table (Table55 and Figure Figure5A),5A), unaltered IFN-γ and TNF-α in bronchoalveolar lavage fluid (BALF) (Figure (Figure5,5, B and C), as has been described previously (28), lung tissue inflammation (Figure (Figure5D),5D), and AHR (Table (Table5).5). Infiltration of human mononuclear cells was observed only around vessels and airways of mice receiving asthmatic PBMCs (Figure (Figure5D).5D). Importantly, quantitative PCR (qPCR) analysis revealed a marked increase in the level of human TIM1 mRNA in the lungs of D. pteronyssinus–sensitized and –challenged asthmatic hu-PBMC SCID mice compared with nonallergic donor control mice, when normalized to human ribosomal L32 (Figure (Figure5E).5E). The TIM1 mRNA detected likely represents both mucin domain variants of human TIM-1 (having or lacking the 5–amino acid insertion at position 158), as RT-PCR and sequencing analysis have revealed expression of both variants in human PBMC, tonsil, and other tissue samples from multiple donors (our unpublished observation). Examination of murine TIM-1 expression by qPCR revealed low-level expression that was not affected by the treatment protocol (Figure (Figure5E).5E). Induction of the asthmatic phenotype was dependent on consecutive challenges with D. pteronyssinus, as previously described (21), and the presence of human asthmatic cells, since transfer of nonallergic cells had no effect (Table (Table5).5). We did not detect eosinophils or neutrophils in the hu-PBMC SCID system, due to absence of these cell types in the transferred populations, confirming previous observations (29).
We next tested anti-human TIM-1 mAb A6G2 for therapeutic activity in the hu-PBMC SCID model of experimental asthma (Table (Table4).4). Mice treated with anti-human TIM-1 mAb A6G2 had a markedly reduced influx of leukocytes into the airways (Table (Table5).5). Measurement of cytokines in the BALF and lung homogenates of anti-human TIM-1 mAb A6G2–treated mice revealed a strong reduction of human IL-4, whereas no significant changes in the IFN-γ or TNF-α levels were observed (Table (Table55 and Figure Figure5,5, A–C). The reduction in lung inflammation with anti-human TIM-1 mAb A6G2 was comparable to that observed with the anti-human IL-13 mAb, while the isotype control mAb (IgG) and anti-human TIM-1 mAb A3H1 had no effect on these readouts (Table (Table5).5). Anti-human TIM-1 mAb 3H1 binds to human TIM-1 with similar affinity as anti-human TIM-1 mAb A6G2 but binds to a distinct epitope (Table (Table22 and our unpublished observation).
In addition, AHR substantially improved in mice treated with anti-human TIM-1 mAb A6G2 or anti-human IL-13 mAb. Neither the isotype control antibodies nor anti-human TIM-1 mAb A3H1 affected the outcome of AHR, with values comparable to the positive control (Table (Table5).5). Finally, neither anti-human TIM-1 mAb A6G2 nor anti-human IL-13 mAb treatment affected the migration and homing of asthmatic PBMCs from the site of injection, showing that the therapeutic effects in the lung were not due to differences in cell transfer efficiency (data not shown). These data demonstrate the therapeutic efficacy of anti-human TIM-1 mAb A6G2 in a humanized mouse model of asthma.
Having observed upregulation of human TIM1 mRNA in the lungs of asthmatic hu-PBMC SCID mice, we further investigated possible cellular sources of human TIM-1. Due to the relatively low number of human cells migrating into the lungs of hu-PBMC SCID mice, further characterization of TIM-1 expression was carried out in donor cells using ex vivo assays. For this purpose donor CD3+/CD4+ and CD3+/CD8+ T cells, CD14+ monocytes, and CD19+/CD20+ B cells were purified from PBMCs by cell sorting and the expression of TIM1 mRNA was analyzed by qPCR. Human TIM1 mRNA was detected in CD3+/CD4+ T cells from asthmatic donors, but not from nonallergic donors (Figure (Figure6,6, A and B). In contrast, CD3+/CD8+, CD14+, and CD19+/CD20+ cells from asthmatics or nonallergic mice did not express TIM1 mRNA (Figure (Figure6A),6A), while DC populations constitutively expressed low levels of TIM1 RNA that was not affected by the asthmatic phenotype (Figure (Figure6,6, C and D). We were unable to detect surface TIM-1 protein on CD3+ T cells by FACS analysis. Therefore we further investigated the immune cell types infiltrating the lungs of hu-PBMC SCID mice. Using immunohistochemical (IHC) staining for human CD4, CD8, CD20, and CD68, we found that the inflammatory infiltrates consisted predominantly of human CD4+ T cells, while lower numbers of CD8+ T cells were observed (Figure (Figure6E).6E). Occasionally a CD20+ B cell was observed, but no CD68+ macrophages were detected. These results suggest that pathogenic T cells represent a major target of TIM-1 antagonism in the asthmatic hu-PBMC SCID mice.
To further evaluate the mechanism of anti–TIM-1 mAb A6G2 treatment, we examined the effect of TIM-1 blockade on the expression levels of transcription factors indicative of T cell effector subsets including Th1 and Th2 cells and Tregs in the lungs of the hu-PBMC SCID mice (Figure (Figure7,7, A–D). Anti-human TIM-1 mAb A6G2 treatment resulted in the downregulation of the Th2-associated transcription factors GATA-3 and c-Maf and an increase in Treg-associated FoxP3 expression (Figure (Figure7,7, A–C), while Tbet mRNA levels remained undetectable (Figure (Figure7D).7D). The increase in FoxP3 levels was not associated with a detectable increase in IL-10 levels or the number of FoxP3+ cells in BALF (data not shown). Furthermore, mRNA expression of the Th17 cell–associated transcription factors RORgt and RORa were below detection levels. These data are consistent with the BALF cytokine profile indicating that anti-human TIM-1 mAb A6G2 affects the development of a Th2 phenotype required for establishment of asthma.
There are a variety of potential mechanisms of action that might explain the activity of the anti-human TIM-1 mAb A6G2, including the reduction of Th2 T cell numbers and/or suppression of cytokine production by individual Th2 T cells. We therefore determined the frequency of IL-4–producing T cells by intracellular cytokine staining and FACS analysis (Figure (Figure7,7, E and F). Treatment of the cultures with anti-human TIM-1 mAb A6G2 significantly blocked the D. pteronyssinus–induced increase in the frequency of IL-4–producing T cells. Also, polyclonal stimulation of human PBMCs with anti-CD3/anti-CD28 antibodies in the presence of anti-human TIM-1 mAb A6G2 resulted in a strong downregulation of Th2 cytokine production, including IL-4 (Figure (Figure7G),7G), but had no effect on IFN-γ production levels (Figure (Figure7H).7H). To examine whether the observed effects of anti-human TIM-1 mAb A6G2 were due to selective inhibition of Th2 cell proliferation, a BrdU incorporation assay was utilized. Incubation of activated asthmatic T cells with anti-human TIM-1 mAb A6G2 strongly suppressed T cell proliferation (Figure (Figure7I)7I) without causing any apparent cell depletion. Similar results were obtained using anti-CD3/anti-CD28 activated T cells harvested from the spleens of hu-PBMC SCID mice reconstituted with cells from asthmatic donors (Figure (Figure7J).7J). Taken together, these results suggest that the effect of anti-human TIM-1 mAb A6G2 on the frequency of IL-4–producing T cells and Th2 cytokine production is due to suppression of T cell proliferation.
To examine whether anti-human TIM-1 mAb A6G2 affects functional DC–T cell interaction, CD14+ monocytes from allergic asthmatic donors or nonallergic control donors were cultured to derive myeloid DCs. These myeloid DCs were co-cultured with autologous CD4+ T cells. CD4+ T cells and myeloid DCs, when cultured alone for 5 days, did not proliferate (Figure (Figure7K),7K), and IL-5 secretion was negligible (Figure (Figure7L).7L). In contrast, CD4+ T cells when cultured together with myeloid DCs from allergic asthmatic but not nonallergic controls proliferated strongly and produced IL-5 (Figure (Figure7,7, K and L). Anti-human TIM-1 mAb A6G2 reduced proliferation and IL-5 production to background levels (Figure (Figure7,7, K and L), suggesting that D. pteronyssinus–induced T cell activation by DCs critically depends on TIM-1 signaling. These data suggest that human TIM-1 antagonism by mAb A6G2 was able to substantially block myeloid DC–dependant Th2 T cell activation.
TIM1 has been identified as a gene associated with asthma and other diseases in multiple human genetic studies (3). All TIM family proteins are type I cell surface glycoproteins and share common structural motifs, but protein interactions are complex in the TIM family. TIM-1 and TIM-4 appear capable of cation-dependent homophilic and heterophilic interactions utilizing the BED face of the molecule, whereas TIM-2 dimerizes through interaction of BC and CCι loop residues, with residues in the FG loop and G β strand (30). The difference in binding modalities may relate to the length of the CCι loop found in TIM-2 versus TIM-1 or TIM-4 (30) and may also involve differential use of glycosylation sites. TIM-3 uses N- and O-linked glycosylation sites on the BED face and adjacent strands to bind its ligand galectin-9 (31). TIM-3 homodimerization and heterodimerization with other family members has also been reported (26).
The IgV domain in all TIM proteins presents a unique cleft created by the CC′/FG configuration (12, 13). To date, the only ligand interaction ascribed to this cleft is the cation-dependent binding of TIM-1 and TIM-4 to PS (13, 24, 25) and perhaps other phospholipids (32). Recently TIM-3 has also been described as having PS binding capability (33). TIM-1 and TIM-4, but not TIM-3, have a sialic acid–like binding motif that might regulate cleft structure through carbohydrate interaction, either on the mucin or stalk domains (having O- and N-linked glycosylation sites, respectively) or to carbohydrate on another ligand. This sialic acid binding site includes amino acids at the top of the CC′/FG cleft (26). Whether the TIM-1 sialic acid binding motif mediates binding to carbohydrates in a manner that regulates cleft structure is not known. Mutations within the metal ion-dependant ligand binding site (MILBS) at the base of the cleft eliminate PS binding (13). The requirement for cation is confirmed by the observation that chelation with EDTA or EGTA dramatically reduces PS binding.
Interestingly, the observation that TIM-1 protein exhibits both cation-sensitive and cation-insensitive binding to DCs suggests that a novel mode of interaction with DCs may exist (26), as chelation of cations should significantly reduce or eliminate both TIM-1/TIM-4 and TIM-1/PS binding (13, 26). In our study, the cation-insensitive binding of TIM-1 to cultured murine myeloid DCs (Figure (Figure2A2A and Table Table3)3) and freshly obtained murine CD11c+ DCs (data not shown) was blocked by anti-mouse TIM-1 mAb 4A2, a mAb that binds within the F > G region, suggesting that multiple binding interactions may be mediated by this structure. In this regard it is important to first consider the potential for homophilic and heterophilic interactions. TIM-1 homophilic interaction utilizes the BED face of the protein (12) and not the CC′/FG face, and TIM-1/TIM-4 heterophilic interaction is likely to be similar. Furthermore, such an interaction would not be blocked by an antibody directed to the F > G portion of the TIM-1 protein. TIM1 mRNA can be detected by RT-PCR in mouse and human DC populations, although protein expression has not been demonstrated (our unpublished observation). Of note, human TIM1 mRNA was detected in asthmatic and nonallergic PBMC preparations, but expression was not affected by disease state (Figure (Figure6,6, C and D). Additionally, TIM4 mRNA is reportedly absent from mouse bone marrow–derived myeloid DCs (24, 25) and from human PBMC preparations (24), which represent 2 sources of the DCs used in our assays. We have previously shown that anti-murine mAb 4A2 antagonizes TIM-1 function in vivo, providing therapeutic benefit in a murine model of experimental asthma by modulating T cell function (9). We hypothesized that antagonism of the cation-insensitive TIM-1/DC binding using similar mAbs directed against human TIM-1 would be a functionally relevant activity assay. Screening mouse anti-human TIM-1 mAbs revealed that anti-human TIM-1 mAb A6G2 was sensitive to replacement of the human F > G strand/loop with murine sequence and was efficiently able to block the binding of TIM-1–Fc to DCs in FACS assays. Furthermore, anti-human TIM-1 mAb A6G2 also greatly reduced binding of human TIM-1–Fc protein to activated human myeloid DCs or freshly obtained human CD11c+ DCs in the presence of EGTA (Figures (Figures22 and and3),3), just as anti-mouse TIM-1 mAb 4A2 did in the murine system. Most other anti–TIM-1 mAbs tested lacked such activity (Tables 2 and 3). On the basis of its activity in the DC binding assay, anti-human TIM-1 mAb A6G2 was chosen for further analysis in vivo and in vitro. We also examined the functional activity of anti-human TIM-1 mAb A3H1, which bound to the TIM-1 IgV domain with similar affinity as A6G2, but did not block binding to DCs.
The therapeutic efficacy of human TIM-1 mAb A6G2 was tested in the hu-PBMC SCID model of T cell–dependent experimental asthma (22, 23). SCID mice were reconstituted with asthmatic PBMCs from patients with high D. pteronyssinus IgE titers, then challenged with D. pteronyssinus to trigger inflammation, cytokine production, and AHR. The development of asthma in this model led to the upregulation of human TIM1 mRNA in the lungs along with the induction of Th2 cell–associated transcription factors GATA-3 and cMAF and the cytokine IL-4 (Figure (Figure5,5, A and E, and Figure Figure7,7, A and B). Since these human TIM-1 primers did not detect mouse TIM-1, these data indicate expression of human TIM-1 in the inflammatory cells in the lung. Interestingly, mouse TIM-1 was expressed in lung tissue at lower levels than induced human TIM-1, and expression of murine TIM-1 in the SCID mouse lung was not affected by the human inflammatory infiltrate in response to the asthmatic challenge protocol.
Similar to the therapeutic benefit seen in the murine OVA-induced asthma model with anti-murine TIM-1 mAb 4A2, treatment of asthmatic hu-PBMC SCID mice with the anti-human TIM-1 mAb A6G2 strongly reduced BALF and tissue levels of the Th2 cytokine IL-4, reduced lung inflammation, and prevented AHR (Figure (Figure55 and Table Table5).5). These effects resulted from a suppression of the Th2 response rather than an induction of the Th1 response, since neither neutrophil numbers nor IFN-γ levels were increased in the lungs of treated mice. As a positive control for the in vivo model, we chose anti-human IL-13 mAb treatment, a therapeutic modality known reduce Th2-dependent allergic asthma responses and currently being evaluated in human clinical trials (34, 35). Notably, anti-human TIM-1 mAb A6G2 treatment resulted in even stronger therapeutic benefit than anti–IL-13 mAb treatment in this model. Furthermore, we detected a decrease in lung GATA3 and cMAF mRNA and IL-4 protein in BALF. Interestingly, anti-human TIM-1 mAb A6G2 treatment led to an increase in Foxp3 mRNA levels, a result which corroborates a recent report that agonist anti–TIM-1 mAb caused downregulation of FoxP3 (36). However, we did not detect an increase in the number of FoxP3+ cells in the lung, suggesting that the effect of human TIM-1 antagonism was due to blockade of the Th2 response rather than an induction of Tregs. Nonetheless, these findings indicate a functional effect of TIM-1 antagonism in addition to modulation of Th2 cells that requires further investigation.
A number of reports demonstrate expression of murine TIM-1 on Th2 CD4+ T cells (32) and B cells (9, 33). Expression of human TIM-1 has not been as conclusively demonstrated, with only a few studies showing expression on activated T cells (34) and kidney epithelium (37). As TIM1 mRNA was seen to be selectively expressed in CD4+ T cells of asthmatic patients, the increase in human TIM1 mRNA in lungs of SCID mice is suggested to result from the subsequent expansion of D. pteronyssinus–specific T cells by antigen challenge. We postulate that the observed suppression of the Th2 phenotype was a direct effect of anti-human TIM-1 mAb A6G2 on T cells, rather than effects from bystander cells. However, we were not able to detect cell surface expression of TIM-1 on T cells by FACS, perhaps indicating that cell-cell contact, transient Ca++ flux (12), or other signals are required to maintain expression. This issue requires further investigation.
Additional in vitro experiments showed that anti-human TIM-1 mAb A6G2 reduces the number of IL-4–producing CD3+ T cells, inhibits IL-4 production, and suppresses T cell proliferation in response to D. pteronyssinus stimulation (see Figure Figure7).7). Importantly, anti-human TIM-1 mAb A6G2 blocked the activation of already-primed, D. pteronyssinus–specific T cells by DCs in vitro, which again suggests a direct effect on T cell activation and further suggests that it might be possible to directly target pathogenic memory T cells in vivo through antagonism of TIM-1.
The striking impact of anti-human TIM-1 mAb A6G2 on cytokine production, lung inflammation, and AHR is consistent with the observation that TIM-1 is genetically linked to human atopic disease (38, 39). The TIM1 gene is also associated with murine atopic immune responses (7), and anti–TIM-1 mAbs have been shown to affect murine allergic inflammation and other pathologies (8, 9, 40, 41). As shown here, anti-mouse TIM-1 mAb 4A2 and anti-human TIM-1 mAb A6G2 both target the CC′/FG cleft of the IgV domain, and both have therapeutic activity in asthma models. This may be due to their ability to block TIM-1 binding to PS, DCs, or both. Interestingly, our analysis of RMT1-10, a second anti-mouse TIM-1 mAb with therapeutic activities (40, 41), showed that while RMT-10 was capable of blocking mouse TIM-1/DC binding, it did not affect PS binding (data not shown). These data suggest that the antagonism of the TIM-1/DC interaction is important for therapeutic effects, and our data suggest the existence of an additional, uncharacterized ligand on DCs. We propose that TIM-1 interaction with PS may serve an interrogatory function, by which T cells monitor the health and integrity of cells with which they interact. Thus, healthy cells will express low levels of PS, generally bound up in cis cell surface interactions (24). In this regard the activity of agonist anti–TIM-1 mAbs described in the murine system (9, 36) is of particular interest, since such mAbs may recapitulate the pathway triggered by a non–PS ligand/TIM-1 interaction. To our knowledge, such agonist anti–TIM-1 mAbs have not yet been described in the human system.
In summary, we have demonstrated that the antagonism of TIM-1 activity blocks the asthmatic response in a humanized mouse model, providing functional evidence to support the hypothesis that TIM-1 is genetically linked to human atopic disease (6). We have shown that modulation of TIM-1 signaling by blocking TIM-1 binding strongly inhibits T cell pro-proliferative and effector activities, which are critical for aggravation of allergic disease. Furthermore, we have outlined the structure/activity relationships that define therapeutic anti-human TIM-1 mAbs using a comprehensive panel of biochemical and functional analyses. Such progress in our understanding of TIM-1 activity will support ongoing efforts to identify novel TIM-1 ligands and will guide future development of therapeutics for asthma and other diseases.
Human TIM1 was PCR amplified from a kidney cDNA library using the oligonucleotide primers KID-309 (5′-TAGCGGCCGCAGGCTGATCCCATAATG-3′) and KID-311 (5′-TAGCGGCCGCTTTCCAGGGACTATTCTC-3′), yielding the allelic variant with the 5–amino acid insertion at position 158 (MTTVP) described by McIntire et al. (7) and cloned NotI/NotI into vector pEAG347, a mammalian expression vector suitable for transient transfections, to create vector SJR102. The extracellular domain was subcloned in-frame with a human IgG1-Fc coding sequence to create PEM151, in vector pv90, an expression vector suitable for transient transfections or for selection in CHO cells. The human TIM1 IgV domain was PCR amplified from SJR102 using the oligonucleotide primers SC1-793 (5′-GCGGCCGCTCTAGAATGCATCCTCAAGTGGTCATCTT-3′) and SC1-794 (5′-ACTAGTGTCGACGGGTGGCACAATCTCCAATGATA-3′) and cloned in-frame with a human IgG1-Fc coding sequence into vector pv90, to create PEM112. Human TIM-1–Fc and TIM-1–IgV–Fc proteins were purified from cell culture supernatants using protein-A column chromatography and size exclusion chromatography to isolate a homogeneous preparation. Murine constructs and proteins were produced as previously described (9).
RBF mice were immunized with full-length purified human TIM-1–Fc protein. The primary immunization was with 25 μg protein in 25 μl Protein A Sepharose beads (Amersham Biosciences) in one flank delivered s.c. and 25 μl CFA/PBS given i.p. The second immunization was similar, except that IFA/PBS was used in place of CFA/PBS. Three days before fusion, 50 μg protein in 50 μl Protein A Sepharose beads was delivered s.c. along with 50 μl RIBI/PBS given i.p. Finally, 1 day before fusion, 50 μg protein was given in PBS i.v. The spleen was harvested, and splenic B cells were fused to FL-653 myeloma cells. After limiting dilution, hybridoma clones were grown out and screened for mAb binding, in supernatant, to purified human TIM-1–IgV–Fc protein in a standard ELISA format. Positive clones were subcloned, expanded, and used for the generation of purified monoclonal antibodies using standard Protein A chromatographic techniques. All purified mAbs were checked by size exclusion chromatography for homogeneity and were assayed for endotoxin, which was routinely less than 0.05 endotoxin units per milligram. Rat anti-mouse TIM-1 mAbs were produced as described in ref. 9.
EC50 values for anti-human TIM-1 mAbs were determined by ELISA analysis. Briefly, ELISA plates (Corning Costar) were coated with 0.2 μg/ml human TIM-1–Fc overnight at 4°C and then blocked for 1 hour at 22°C with PBS/0.5% casein. Plates were thoroughly washed with PBS/0.1% Tween-20. Anti-human TIM-1 mAbs were added across a range of concentrations starting at 10 μg/ml and using 1:3 dilutions, all in PBS/0.5% casein, and allowed to incubate for 1 hour at 22°C. The plates were washed again, and then a 1:1,000 dilution of HRP-conjugated goat anti-mouse IgG (H+L; Jackson Immunoresearch Laboratories) was added in PBS/0.5% casein for an additional hour at 22°C. After a final wash, the plates were developed using substrate solution mix (R&D Systems) and stopped using 2N H2SO4. Plates were read at 450 nM using a microplate reader and analyzed using SoftMax Pro (Molecular Devices). Relative EC50 values were determined from the binding curves. Rat anti-murine TIM-1 mAbs were analyzed similarly using immobilized murine TIM-1 fusion proteins (9).
In order to determine the region within the IgV domain bound by anti-human TIM-1 mAb A6G2, a mutant molecule was made in which the F and G strands and intervening loop within the IgV domain of human TIM-1 were replaced by sequence from mouse TIM-1. Full-length human TIM-1 was cloned into a pNE001-derived EBV expression vector to create pEAG2182. pEAG2182 was subjected to site-directed mutagenesis (QuikChange; Stratagene) to mutate the human F strand/loop sequence to the mouse sequence using oligonucleotides KM1-685 (5′-GCTGTGTCTGACAGTGGCCTATATTGTTGCCGTGTTGAGATCCCTGGGTGGTTCAATGACATG-3′) and KM-686 (5′-CATGTCATTGAACCACCCAGGGATCTCAACACGGCAACAATATAGGCCACTGTCAGACACAGC-3′) to create pEAG2193. pEAG2193 was further mutagenized to replace the rest of the F > G loop and G strand using oligonucleotide primers KM1-687 (5′-CCTGGGTGGTTCAATGACCAGAAG GTCACCTTTTCATTGCAAGTTAAACCACCTAAGGTCACGACTAC-3′) and KM1-688 (5′-GTAGTCGTGACCTTAGGTGGTTTAACTTGCAATGAAAAGGTGACCTTCTGGTCATTGAACCACCCAGG-3′) to create pEAG2198. Mutated and wild-type constructs were used to transfect 293 cells, along with an EGFP expression plasmid.
For analysis of mAb binding to mutated forms of human TIM-1, 293 cells were grown to approximately 80% confluence in DMEM/10% FBS, then transfected using Effectene, following the manufacturer’s instructions (Qiagen). EGFP expressing plasmid was cotransfected with the TIM-1–expressing plasmids. After 48 hours, cells were washed and harvested into PBS/0.5% BSA/0.1% sodium azide for FACS analysis. Mouse anti-human TIM-1 mAbs or isotype control were added for 1 hour on ice, the cells were washed with PBS, then fluorescently labeled goat anti-human IgG-Fc antisera was added for an additional hour, before a final washing and fixation in PBS/1% paraformaldehyde. EGFP-positive cells were gated to ensure that only transfected cells were analyzed. FACS data were collected on a FACSCaliber Instrument (BD Biosciences) and analyzed using FloJo software after gating on the EGFP-positive cell population.
Plates (96-well; Corning Costar 3590) were coated with 10 μg/ml PS in 100 μl methanol and placed in an exhaust hood until dry, then blocked with 200 μl/well of 1% BSA in Tris buffer (25 mM Tris, 137 mM NaCl, pH 7.2) for 1 hour. Wells were washed 4 times with 0.05% Tween-20 in Tris buffer. Murine TIM-1–Fc or human TIM-1–Fc proteins in 100 μl 1% BSA/Tris were bound for 1 hour to PS-coated plates across a range of concentrations using 1:3 dilutions starting from 100 μg/ml. After 3 washes with 0.05% Tween-20/Tris buffer, 100 μl/well of a 1:1,000 dilution of HRP-conjugated goat anti-human IgG-Fc antibody (Jackson Immunoresearch Laboratories) in 1% BSA/Tris was added for 1 hour. Following 4 additional washes with 0.05% Tween-20/Tris, the plates were developed using 50 μl/well substrate solution (R&D Systems) then stopped by adding 100 μl 2N H2SO4. Plates were read on a microplate reader at 450 nM. We determined the dependence of PS/TIM-1 binding on the presence of cation by using Tris buffer without additional Ca++ or Mg++ added, or by adding 1 mM EGTA to the binding solution. Anti-mouse TIM-1 or anti-human TIM-1 mAbs were used to compete for binding to PS in Tris buffer containing 1 mM Ca++ and 1 mM Mg++ at a concentration of 10 μg/ml.
Mouse DCs were cultured from bone marrow to yield myeloid DCs, as previously described (42), or were analyzed as CD11c+ DCs from splenocyte preparations. Human myeloid DCs were cultured from CD34+ stem cells as instructed by the supplier (Stem Cell Technologies) or were analyzed as CD11c+ DCs from PBMC preparations. Anti-mouse CD11c mAb and anti-human CD11c mAb were purchased (BD Biosciences) for use in assessing purity (routinely > 90%) and for gating cell populations. In some experiments DCs were stimulated with 100 ng/ml LPS (Sigma-Aldrich) for 48 hours. TIM-1–Fc fusion proteins were incubated with cell samples at various concentrations for 1 hour on ice, in the presence or absence of 1 mM EGTA, and in the presence or absence of 10 μg/ml anti–TIM-1 mAbs or isotype control mAbs (rat IgG2a [BD Biosciences] and mouse mAb MOPC21 [ATCC]). TIM-1–Fc fusion protein binding was analyzed by FACS after incubation using fluorochrome-conjugated goat anti-human IgG-Fc (Jackson Immunoresearch Laboratories). Cells were fixed in 1% paraformaldehyde immediately after staining.
Female SCID mice (6–8 weeks old; C.B-17 SCID) were obtained from Harlan Winkelmann (Borchen) and maintained under pathogen-free conditions. All animal experiments were performed according to The guidelines for the care and use of experimental animals prepared by the Society for Laboratory Animal Sciences (GV-SOLAS), performed in accordance with German law under “Genehmigung von Versuchsvorhaben V 54 - 19 c 20-15 (1) MR20/13-Nr.75/2007,” and was approved by the Regierungspra_sidium Gieβen, the regional ethics committee responsible for the University of Marburg, Marburg, Germany.
Asthmatic patients sensitized to house dust mite allergen (D. pteronyssinus) were identified by elevated serum D. pteronyssinus–specific IgE antibody titers. Human serum IgE antibody titers were measured by fluorescence enzyme immunoassay (Pharmacia CAP System; Pharmacia). A total of 22 allergic and asthmatic patients suffering from moderate to severe asthma according to international guidelines (Global Initiative for Asthma) with antibody titers of at least 410 ng/ml for total IgE and at least 20 ng/ml for anti–D. pteronyssinus IgE antibodies were selected as donors and referred to as asthmatics. A total of 15 nonallergic healthy subjects with total serum IgE concentrations of less than 17 ng/ml and anti–D. pteronyssinus IgE of less than 0.8 ng/ml were referred to as nonallergic donors. All blood samples were obtained with written informed consent. Heparinized blood (200–250 ml) was collected from asthmatic and nonallergic donors, and mononuclear cells were purified by Histopaque (Sigma-Aldrich) density gradient centrifugation. The human donor PMC-collection was approved by the ethics committee of Graubunden, Switzerland, responsible for the Hochgebirgsasthmaklinik, Davos, Switzerland.
Three independent experiments were performed. Each experiment included 6–8 animals per group that received PBMCs from at least 5 asthmatic or nonallergic donors. SCID mice received 2 × 107 PBMCs i.p. on day 1. Mice also received 100 μg of purified house dust mite extract (Allergopharma Joachim Ganzer), referred to as D. pteronyssinus, with 14 mg/ml of aluminium hydroxide adjuvant (Pierce Biotechnology) on the same day as the cell transfer and on days 7 and 14. All animals were aerosol challenged with 200 μg D. pteronyssinus diluted in 5 ml PBS for 20 minutes on days 16, 18, 20, 23, and 25. Animals also received 100 μg i.p. injections of control IgG1 antibody (MOPC21), anti-human IL-13, anti-human TIM-1 mAb A6G2, or anti-human TIM-1 mAb A3H1 diluted in 200 μl PBS, or PBS alone (Figure (Figure4,4, A and B).
Lung tissue was harvested, and snap-frozen tissue was processed for RNA extraction using the RNeasy protocol (Qiagen). Contaminating genomic DNA was removed by DNase treatment using the DNA-free kit (Ambion), and cDNA synthesis was performed with 500 ng of total RNA using the SuperScript III Reverse Transcriptase (Invitrogen). qPCR was performed using the QuantiTect SYBR Green PCR Kit (Qiagen). Primers specific for human and murine TIM-1 and human GATA-3, T-bet, FoxP3, cMAF, RORγt, and RORα are described in Table Table6.6. The mRNA copy numbers were normalized and expressed relative to the human/mouse ribosomal L32 as the housekeeping gene. Following an initial denaturation step of 15 minutes at 95°C, 45 PCR cycles of 94°C for 15 seconds, 60°C for 30 seconds, and 72°C for 15 seconds were performed. Finally, product homogeneity was verified by melting curve analysis. Real-time qPCR analysis was performed using a Light-Cycler System (Roche). All standard procedures were performed as per the manufacturer’s instructions.
Noninvasive measurement of mid-expiratory airflow (EF50) to methacholine was measured 24 hours after the last D. pteronyssinus aerosol challenge using head-out body plethysmography as described previously (43). Dose-response curves of airway reactivity to methacholine was assessed using head-out body plethysmography (see Supplemental Methods).
Forty-eight hours after the last allergen challenge, BALF was collected and analyzed as previously described (44). The total number of leukocytes was determined by using a Casy TT cell counter (Schaerfe Systems). Cells were differentially stained with Diff-Quick (DADE Diagnostics).
Human IL-4 (BenderMed) with a detection limit of 0.25 pg/ml and IFN-γ (OptEIA; BD Biosciences) with a detection limit of 4.7 pg/ml were measured in the cell-free lavage fluid or cell culture according to the manufacturer’s instructions. For detection of human cytokines in lung homogenates, snap-frozen lungs were thawed, weighed, crushed, and homogenized in 500 μl of T-PER (Thermo Scientific) containing Complete Mini Protease Inhibitor Cocktail tablets (Roche; 1 tablet/10 ml of T-PER stock reagent). Lung homogenates were centrifuged at 9,000 g for 10 minutes at 4°C. Total protein concentrations in supernatants were determined using a BCA kit (Thermo Scientific), and human Cytokine 11-Plex kit reagents were used to evaluate amounts of IL-1β, -2, -4, 5, -6, -8, -10, and -12 (p70), TNF-α, TNF-β, and IFN-γ expression patterns (BenderMed). Specific cytokine protein concentrations were expressed as pg/mg total lung protein.
Mononuclear cells were purified by Histopaque (Sigma-Aldrich) density gradient centrifugation. PBMCs (1 × 106/ml) were either stimulated with D. pteronyssinus (500 ng/ml) for 72 hours for staining IL-4–positive CD3 cells by FACS or incubated with mAb CD3/CD28-coated plates (BD Biosciences) at a concentration of 500 ng/ml and 1 μg/ml respectively for 24–48 hours. Cells were grown in medium alone or with anti-human TIM-1 mAb A6G2 (1 μg/ml) or control IgG (1 μg/ml). The supernatants were further processed for cytokine analysis.
Histopaque-purified PBMCs were resuspended in PBS, stained with fluorochrome-labeled mAbs, and sorted with the MoFlo flow cytometer (DakoCytomation). For the sorting procedure, cells were gated with a combined live gate, according to scatter characteristics and dead cell exclusion via DAPI stain at 100 ng/ml final concentration (Sigma-Aldrich). T cells were discriminated using CD3 and CD4 or CD8 antibody surface staining, monocytes using CD45 and CD14 staining, B cells using CD19 and CD20 staining (BD Biosciences), myeloid DCs using CD11c and HLA-DR, and plasmacytoid DCs using HLA-DR and BDCA-2 (BD Biosciences). Sorting was performed in 1.5-ml plastic tubes at room temperature without external cooling. The sort purity was greater than 95%.
CD14+ and CD4+ T cells were isolated from asthmatic and nonallergic donors using whole blood CD14 and CD4 MicroBeads (Miltenyi Bitotech). CD4+ and CD14+ cells were isolated directly from whole blood using automated magnetic cell sorting (autoMACS Magnetic Cell Sorting; Miltenyi Bitotech). Isolated CD14+ cells were cultured in RPMI complete medium with 100 ng/ml of GM-CSF and IL-4 (Miltenyi Bitotech) at 37°C in 5% CO2 for 6 days. DCs were checked for differentiation and maturation markers CD83, CD86, and HLA DR (BD Biosciences) before coculture with T cells. Purified autologous CD4+ T cells were isolated (Miltenyi Bitotech) and were co-cultured with myeloid DCs at a 1:10 ratio in complete medium with or without D. pteronyssinus, D. pteronyssinus plus anti-human TIM-1 mAb A6G2, and D. pteronyssinus plus control IgG in 96-well plates for a further 5 days. Control wells containing only CD4+ T cells and only myeloid DCs were also prepared. Proliferation and cytokine production were analyzed by BrdU incorporation assay (Roche) and ELISA (IL-5 and IFN-γ), respectively. The detection limit for the IL-5 ELISA was 1.6 pg/ml and for the IFN-γ ELISA was 1 pg/ml.
Histopaque-purified PBMCs from asthmatic donors were cultured in triplicate at a concentration of 106 cells/ml. Six hours prior to harvesting, Brefeldin A (Sigma-Aldrich; 10 μg/ml) was applied to the cell culture. At the 72-hour time point, cells were washed twice with PBS, counted, and 106 cells were stained with PerCP-conjugated anti-CD3 mAb (BD Biosciences). Cells were then washed, fixed with Cytofix (BD Biosciences), permeabilized with 0.3% Saponin in PBS, and stained with APC-conjugated anti-human IL-4 antibody (BD Biosciences). Cells were gated using forward- and side-scatter, and surface expression of CD3 and intracellular IL-4 was analyzed. mAbs were titered prior to use to determine optimal concentrations. Analysis was performed using Flow Cytometry instrumentation and software (BD Biosciences).
Cell proliferation assays were performed using a BrdU labeling and detection kit (Roche). Briefly, 106 mononuclear cells were isolated from mouse spleen or donor PBMCs were incubated in 96-well plates for 24 hours in culture medium alone or with anti-human CD3/CD28 mAbs. BrdU was then added to a final concentration of 10 μM/l. After incubation for an additional 24 hours, DNA synthesis was assayed according to the manufacturer’s instructions. BrdU-labeled DNA was detected using a luminometer.
IHC staining was performed on paraffin-embedded lung tissue. Following rehydration in graded alcohol, tissue slices (3 μm) were treated with either citrate (CD8, CD20, and CD68) or EDTA (CD4) solution in a microwave oven for antigen retrieval according to the antibody manufacturer’s instructions. Anti-human CD4 (clone 4B12; Novocastra), anti-human CD20 (clone L26), anti-human CD68 (clone PG-M1; both Dako Cytomation), and anti-human CD8 (clone SP16; NeoMarkers) were used for the detection of specific human mononuclear cell types. The Histostain Plus kit (Invitrogen) was used for the detection of bound primary antibody and diaminobenzidine was used as chromogenic substrate.
Snap-frozen lungs were thawed, weighed, crushed, and homogenized in 500 μl of T-PER (Thermo Scientific) containing Complete Mini Protease Inhibitor Cocktail tablets (Roche; 1 tablet/10 ml of T-PER stock reagent). Lung homogenates were centrifuged at 9,000 g for 10 minutes at 4°C. Total protein concentrations in supernatants were determined using a BCA kit (Thermo Scientific).The human Cytokine 11-Plex kit reagents were used to evaluate IL-1β, -2, -4, 5, -6,-8, -10, and -12 (p70), TNF-α, TNF-β, and IFN-γ expression patterns (BenderMed). Specific cytokine protein concentrations were expressed as pg/mg total lung protein.
The results are presented as mean values ± SEM of 6–8 mice/cohort unless otherwise stated. The Mann-Whitney U test was used to determine the level of significant difference between groups. A P value of 0.05 or lower was considered significant.
We thank Patricia McCoon, Steven Miklasz, and Alexey Lugovskoy for scientific input and the provision of important reagents. We acknowledge Jan Dudda for scientific input and critical review of the manuscript. We thank Joseph Amatucci, Wenjie Cheng, Attila Fabian, Judy George, Lourdes Pablo, Leticia Tarilonte, Greg Thill, Mohammad Zafari, Stefani Achenbach, Anja Spies, and Heiki Geibel for their technical expertise. We thank Andreas Neubauer and Cornelia Brendel for their scientific expertise and Thorsten Volkmann and Gavin Giel for technical assistance with the MoFlo cell sorting. This study was supported in part by the SFB/TR22 grant, project Z2, provided by the Deutsche Forschungsgemeinschaft.
Conflict of interest: Yen-Ming Hsu, Gerard R. Majeau, Ellen Garber, Yan Gao, Chioma Nwankwo, Hellen Kim, Véronique Bailly, and Paul D. Rennert are employees and stockholders of Biogen Idec Inc.
Citation for this article: J Clin Invest. 2010;120(8):2767–2781. doi:10.1172/JCI39543.