|Home | About | Journals | Submit | Contact Us | Français|
Much is known about the neurobiology of memory storage for learned fear. In contrast, the molecular mechanisms underlying extinction of fear memory are just beginning to be delineated. Here, we investigate the role of protein kinase A (PKA) in extinction of memory for contextual fear by using conventional and temporally regulated transgenic approaches that allow us to inhibit PKA activity in neurons within brain regions thought to be involved in extinction. Strikingly, reduction of PKA activity facilitated the development of extinction, without interfering with storage of the original fear memory. Moreover, inhibition of PKA facilitated extinction of both recent and remote contextual fear memories. The finding that PKA, which is required for the acquisition of fear memory, is a constraint for extinction provides the first genetic support for the idea that fear extinction is itself a genuine learning process with its own specific molecular requirements, rather than simply the erasure of a previously learned process. Further, these experiments represent the first genetic evidence that protein kinases may be constraints for the extinction of fear.
Many studies of the neural and genetic basis of memory have demonstrated that fearful memories can form quickly and are extremely difficult to eliminate (Maren and Quirk, 2004; Fanselow and Poulous, 2005; Phelps and LeDoux, 2005). In the laboratory, fear is created through pavlovian conditioning paradigms, in which a biologically neutral stimulus such as a tone or a context [the conditioned stimulus (CS)], is paired with an aversive event [the unconditioned stimulus (US)], such as a mild footshock in rodent studies. Memory for the CS–US association is robust and long lasting, but if the CS is presented repeatedly in the absence of the US, the fear response weakens through a process known as experimental extinction (for review, see Myers and Davis, 2002; Delamater, 2004). Numerous studies have suggested that extinction is an active learning process that results in new memories that compete with and suppress the expression of memories formed during conditioning (Pavlov, 1927; Bouton, 1993; Rescorla, 2001; Bouton et al., 2006). Indeed, the original memory is not eliminated during extinction and extinguished fear responses return with time (Pavlov, 1927; Lattal and Abel, 2004; Rescorla, 2004). Relatively little is known about the molecular mechanisms that mediate extinction and how these mechanisms compare with those that mediate initial memory storage.
Much is known about the molecular mechanisms and neural systems that underlie the formation of fear memories (Abel and Lattal, 2001; Tonegawa et al., 2003; Rodrigues et al., 2004; Fanselow and Poulos 2005). Extinction appears to rely on many of these same molecular and neural systems, but a number of studies suggest that extinction may also engage different processes (Lattal and Abel, 2001; Cain et al., 2002; Marsicano et al., 2002; Davis et al., 2006; Lattal et al., 2006). One molecular pathway that regulates memory formation across species is the cAMP/protein kinase A (PKA) signaling cascade. This pathway is critical for initial memory formation (Byrne and Kandel, 1996; Abel et al., 1997; Siwicki and Ladewski, 2003), but its role in extinction remains unclear (Koh and Bernstein, 2003; Szapiro et al., 2003; Cammarota et al., 2005; McNally et al., 2005; Tronson et al., 2006). In the following experiments, we take a transgenic approach to the study of PKA and extinction. This approach allows us to regulate PKA in a molecular and cell-type specific manner that is difficult with pharmacological approaches.
We assessed the role of neuronal PKA in the extinction of context-evoked fear by using transgenic mice that express a dominant-negative form of PKA from birth and transgenic mice in which the expression of this same dominant-negative form of PKA could be temporally regulated using the tetracycline system. Both approaches revealed that reduction of PKA activity in fore-brain neurons facilitates extinction of contextual fear without affecting the original fear memory. Moreover, the use of the tetracycline regulated system enabled us to show that inhibition of neuronal PKA activity acutely in the adult forebrain was sufficient to cause impairments in fear memory formation. Together, these data define opposite roles for PKA in the formation of initial memories and extinction memories, and they provide strong genetic evidence for the idea that acquisition and extinction are distinct learning processes.
For some of our experiments, we used previously characterized transgenic mice referred to as calmodulin-dependent protein kinase IIα (CaMKIIα)-R(AB) that expressed the dominant-negative form of PKA R(AB) in forebrain neurons from birth (Abel et al., 1997). Specifically, we used 8- to 12-week-old male and female transgenic R(AB) mice from line 2 and wild-type littermates as controls. For genotyping R(AB) transgenic mice, tail DNA was prepared and analyzed by Southern blotting using a transgene-specific probe as described previously (Abel et al., 1997). Bitransgenic mice in which the expression of R(AB) could be temporally limited by the tTA system were used for some experiments. To generate mouse lines carrying R(AB) under the control of the operator sequences to which the tetracycline-controlled transactivator binds, the cDNA encoding R(AB) was subcloned into the EcoRV site of pMM400 (Mayford et al., 1996b). The tetO-R(AB) transgene [R(AB) cDNA under the control of tetracycline operator sequences] was purified away from vector sequences and microinjected into pronuclei of B6/CBA F1 zygotes that were then implanted in pseudopregnant females. Founders were back-crossed to C57BL/6J mice. Fourteen tetO-R(AB) transgenic mouse lines were established. To generate bitransgenic animals, mice from these 14 lines were crossed to mice from a line that carried the tetracycline-controlled transcriptional transactivator tTA driven by the CaMKIIα promoter (line B) obtained from Mark Mayford (Scripps Institute, La Jolla, CA) (Mayford et al., 1996b). Levels of expression by in situ hybridization, PKA activity assays in extracts from different brain regions, and long term memory for contextual fear conditioning were assessed in bitransgenic mice from the different tetO-R(AB) lines (data not shown). The tetO-R(AB) mouse line showing the highest level of expression of R(AB) and the most profound deficits in long-term memory for contextual fear conditioning (line 7184) was selected for the experiments described in this paper. We used 8- to 12- week-old male and female double transgenic mice and wild-type littermates as controls (no sex differences were observed). For genotyping tTA line B and tetO-R(AB) transgenic mice, tail DNA was prepared and analyzed by Southern blotting using transgene-specific probes. Mice had access to food and water ad libitum and were maintained on a 12 h light/dark cycle with behavioral testing occurring during the light phase. All mice were bred onto a C57B/6J background for more than 10 generations. TetO-linked R(AB) expression was suppressed using mouse chow containing 40 mg/kg doxycline (Dox) (Bio-serve, Frenchtown, NJ). All experiments were conducted according to National Institutes of Health guidelines for animal care and use and were approved by the Institutional Animal Care and Use Committee of the University of Pennsylvania.
Mouse brains were dissected and flash frozen in 2-methylbutane. Sagittal cryostat sections (20 µm) were fixed and hybridized as described previously (Abel et al., 1997). [α-35S]-dATP-labeled, transgene-specific oligonucleotides were used to detect mRNA in CaMKIIα-R(AB) transgenic (5′-GCAGGATCC-GCTTGGGCTGCAGTTGGACCT-3′) and tetO-R(AB) bitransgenic mice (5′-GGTGGGGGGTCCTCTAGATCCGCAGGATCCGC-3′). Slides were exposed to Kodak (Rochester, NY) Biomax autoradiographic film. For detection of Nissl bodies, sections on slides were stained with thionin.
In vitro PKA activity assays were performed as described previously (Abel et al., 1997). Briefly, hippocampi, prefrontal cortex, temporal cortex, amygdalae, and cerebellum were dissected and homogenized in 300, 200, 300, 100, and 400 µl, respectively, of ice-cold extraction buffer [PBS containing the following (in mm): 1 EGTA, 1 EDTA, 0.1% Triton X-100, 5 DTT, 0.5 PMSF, and a mixture of proteases inhibitors]. For amygdalar extracts, amygdalae from two mice were pooled. The extracts were then diluted to a protein concentration of 2 mg/ml (Bradford, 1976) and kept in ice until use. PKA activity was assayed in duplicate at 30°C for 5 min as described previously (Clegg et al., 1987). To some reactions, cAMP (5 µm) and/or protein kinase inhibitor (40 µg/ml) were added. PKA activities (picomoles [γ-32P]ATP per minute per milligram of protein) were calculated relative to the background activity without added substrate.
Fear conditioning experiments were performed in standard conditioning chambers (Med Associates, St. Albans, VT). Mice were handled for two consecutive days for 2 min each day. On the day of acquisition, all mice that were going to be subjected to extinction 24 h later or 4 weeks later, were placed into the conditioning chamber without a cue and received a 2 s, 1.5 mA footshock at 2 and 2.5 min after placement into the chamber as described previously (Lattal and Abel, 2004). Mice were removed from the chamber 30 s after the second shock. During the daily extinction sessions, mice were re-exposed to the conditioning context for 3 min (see Fig. 1) or 24 min (see Figs. 2, ,5)5) in the absence of shock and freezing behavior [defined as complete immobility except for breathing (Fanselow, 1980)] was scored every 5 s for each mouse. In the experiments in which the role of PKA in fear memory formation in the adult was assessed, on the training day, mice were placed in the conditioning chamber and the cue (white noise) was activated from 2 to 2.5 min. The last 2 s of the cue were paired with a 1.5 mA footshock and after 30 additional seconds the mouse was returned to the home cage. Different sets of mice were used for testing contextual and cued conditioning. Conditioning was assessed by scoring freezing behavior. Contextual conditioning was assessed for five consecutive min in the chamber in which the mice were trained 1 h and 24 h after training. Different sets of mice were used for the 1 and 24 h retention tests. For testing cued conditioning, mice were placed in a novel context (another conditioning chamber with a smooth flat floor, altered dimensions, and a novel odorant) for 2 min (pre-CS) followed by exposure to the cue from 2 to 5 min (CS), 24 h after training.
Student’s t tests were used to analyze PKA assays data and the behavioral data from experiments aimed to assess the role of PKA in memory formation in the adult. α was set to 0.05. Repeated-measures ANOVAs were used to analyze the effect of genotype and trials during extinction. Reliable interactions were explored using Fisher’s tests.
To determine whether extinction of contextual fear depends on PKA activity, we first used previously characterized transgenic mice that express a dominant-negative form of PKA, R(AB), under the control of the CaMKIIα promoter (Abel et al., 1997; Bourtchouladze et al., 1998; Rotenberg et al., 2000; Woo et al., 2000a, 2002, 2003). These CaMKIIα-R(AB) transgenic mice are a valuable tool to investigate the role of PKA in extinction of fear because PKA activity is selectively reduced in neurons of forebrain structures known to be relevant for fear extinction such as the amygdala, prefrontal cortex, and hippocampus (Delamater, 2004; Barad et al., 2006; Quirk et al., 2006) because of R(AB) expression from birth (Abel et al., 1997) (Fig. 1A). We found that CaMKIIα-R(AB) transgenic mice show normal memory formation for contextual fear conditioning in response to a stronger fear conditioning training protocol consisting of two massed high intensity footshocks (Woo et al., 2000b). Therefore, CaMKIIα-R(AB) transgenic mice and wild-type littermates received contextual fear conditioning with this protocol. During extinction, mice were re-exposed to the conditioning chamber for 3 min per day for 10 d and levels of freezing were scored (Fig. 1B, extinction). A single 3 min exposure per day to the conditioning context resulted in similar performance early in extinction, but the CaMKIIα-R(AB) transgenic mice showed enhanced extinction in later trials. A genotype by extinction session ANOVA revealed a reliable main effect of extinction session (F(9,189) = 8.7; p < 0.001) and a reliable interaction between genotype and session (F(9,189) = 2.0; p < 0.05). Additional analyses of the interaction demonstrated that although freezing was similar in wild-type and CaMKIIα-R(AB) transgenic mice during the first five sessions of extinction (F(1,21) < 1.0), CaMKIIα-R(AB) mice showed lower levels of freezing compared with wild-type mice during the final five sessions of extinction (F(1,21) = 5.5; p < 0.05), suggesting greater fear extinction. Importantly, CaMKIIα-R(AB) transgenic mice and wild-type littermates exhibited similar performance in a test for spontaneous recovery performed 21 d after extinction (t(21) < 1.0) (Fig. 1B, retest), indicating that the original fear memory remained intact. In subsequent daily re-exposures to the context (Fig. 1B, re-extinction), we observed a trend in which CaMKIIα-R(AB) transgenic mice showed greater re-extinction than wild-type controls [although neither the overall group difference F(1,21) = 3.1 nor the interaction (F(3,63) = 2.0) were reliable], supporting our observations during the initial extinction experiment. Together, these findings indicate that genetic inhibition of PKA facilitated the long-term retention of extinction across short extinction sessions.
Longer re-exposure to the context in the absence of the US is thought to favor development of extinction (Suzuki et al., 2004). We therefore further examined the effects of PKA inhibition on the development of extinction within long extinction sessions. We observed the same pattern of enhanced extinction within longer extinction sessions (Fig. 2) that we observed between brief extinction sessions (Fig. 1B). Twenty four hours after conditioning, CaMKIIα-R(AB) and wild-type mice received a 24 min extinction session for each of four consecutive days. CaMKIIα-R(AB) transgenic mice showed greater extinction over the course of the four 24 min sessions compared with wild-type controls (F(1,22) = 5.1; p < 0.05). Interestingly, at the beginning of each session, performance was similar for CaMKIIα-R(AB) transgenic mice and wild-type littermates, demonstrating recovery from the end of the previous session to the beginning of the next session and suggesting that reduction of neuronal PKA facilitated the development of extinction within the session.
The differences that emerged during extinction shown in (Figures 1 B and ((22 suggest that PKA inhibition facilitated extinction. One caveat to this interpretation, however, is that because PKA was inhibited in CaMKIIα-R(AB) transgenic mice throughout postnatal development, it is difficult to isolate the effects of PKA inhibition directly on extinction, independent of effects on development or on initial conditioning. To demonstrate a direct role for PKA in extinction, we generated bitransgenic tetO-R(AB) mice in which R(AB) expression could be temporally regulated using the tetracycline responsive system.
One transgenic mouse line carried the tetracycline-responsive transcriptional transactivator (tTA) driven by the CaMKIIα promoter to restrict expression to forebrain neurons (line B) (Mayford et al., 1996b). The other transgenic mouse line carried R(AB) driven by the tetO-promoter (Mayford et al., 1996b). Crossing transgenic mice from both mouse lines gave rise to double transgenics [tetO-R(AB)] in which expression of R(AB) can be suppressed by the tetracycline analog Dox (Fig. 3A). To limit R(AB) expression to the adult, transgene expression was suppressed from conception to adulthood by delivering Dox in food at a dose of 40 mg/kg mouse chow (Bejar et al., 2002). Significant levels of R(AB) expression were found 4 weeks after Dox removal in the cortex, hippocampus, striatum, and amygdala (Fig. 3B). This time course of gene expression is similar to what has been described previously for other transgenes regulated by the tTA system (Bejar et al., 2002). Four weeks after Dox removal, PKA activity assays showed significant reduction of PKA activity in the temporal and prefrontal cortex, amygdala, and hippocampus from tetO-R(AB) transgenic mice (p values < 0.05) (Fig. 3C), consistent with the in situ hybridization analysis. Given these results, all subsequent experiments were performed in tetO-R(AB) transgenic mice and wild-type littermates raised on Dox, kept on Dox, and removed from Dox at 8 weeks of age for 4 weeks to allow for R(AB) expression.
It has been reported previously that postnatal transgenic expression of the dominant-negative form of PKA, R(AB), caused selective deficits in long-term memory for contextual fear conditioning (Abel et al., 1997; Bourtchouladze et al., 1998). To assess the role of neuronal PKA in formation of memory in the adult, tetO-R(AB) transgenic mice and wild-type littermates were raised and kept on Dox diet to suppress R(AB) expression throughout postnatal development. At 8 weeks of age, mice were removed from Dox to induce R(AB) expression and 4 weeks later mice were trained and tested in fear conditioning (Fig. 4A). To assess short-term memory, tetO-R(AB) transgenic mice and wild-type littermates were tested 1 h later in the conditioning context (Fig. 4B). TetO-R(AB) transgenic mice and wild-type littermates showed similar levels of freezing, indicating that inhibition of PKA did not impair their ability to learn and have normal short-term memory. To examine long-term memory for contextual fear conditioning, tetO-R(AB) transgenic mice and wild-type littermates were re-exposed to the conditioning context 24 h after training (Fig. 4C). Although both groups showed similar baseline levels of freezing during training, tetO-R(AB) transgenic mice exhibited a significant decrease in freezing compared with their wild-type littermates in the retention test (Fig. 4C). To test long-term memory for cued fear conditioning, tetO-R(AB) transgenic mice and wild-type littermates were presented the conditioning tone in a novel context 24 h after training (Fig. 4D). No differences were observed between groups either before (Fig. 4D, pre-CS) or after the tone (Fig. 4D, CS). Furthermore, tetO-R(AB) mice exhibited high levels of freezing to the tone, indicating that their ability to freeze is intact. No differences in the freezing levels measured immediately after delivering the shock during training were observed between tetO-R(AB) transgenic mice and wild-type littermates in any of the experiments (data not shown). Similar levels of freezing found in a control group of tetO-R(AB) transgenic mice and wild-type (WT) littermates raised on Dox and left on Dox during training and testing 24 h later in the conditioning context indicated that the long-term memory deficits observed were because of the acute inhibition of PKA [baseline: WT (n = 10), 2.0 ± 1.7%; TG (n = 10), 2.5 ± 1.1%; testing: WT (n = 10), 45.6 ± 5.7%; TG (n = 10), 37.9 ± 4.8%; p = 0.83]. Together, these data extend our previous finding that transgenic inhibition of PKA from birth impairs the establishment of contextual fear memories and demonstrate that neuronal reduction of PKA in the adult forebrain is sufficient to cause deficits in memory for contextual fear conditioning. These findings are consistent with previous studies of R(AB) transgenic mice, which have found deficits in contextual, but not cued fear conditioning (Abel et al., 1997).
To examine the acute role of PKA in extinction in the adult and to avoid interference of R(AB) expression during acquisition of fear conditioning, tetO-R(AB) transgenic mice and wild-type littermates were raised and kept on Dox diet to suppress R(AB) expression. At 8 weeks of age, mice still fed Dox were fear conditioned and 24 h later were removed from Dox food to induce R(AB) expression (Fig. 5A). Four weeks after Dox withdrawal, tetO-R(AB) transgenic mice and wild-type littermates were re-exposed to the conditioning context for 24 min over three consecutive days and freezing in the context was measured (Fig. 5B). Similar to what we observed in the CaMKIIα-R(AB) transgenic mice, tetO-R(AB) transgenic mice exhibited greater extinction over the course of the first two 24 min sessions compared with wild-type controls. A genotype by 3 min session block ANOVA revealed reliable group by block interactions during the first 2 d of extinction sessions (F(7,266) values > 2.2; p values < 0.05). Additional analyses of the interactions revealed that although there were no group differences during the first 3 min of each daily extinction session, there were differences during the final 3 min of each session (t(38) values > 2.0; p values <0.05). The similar levels of freezing observed at the beginning of the second and third sessions suggest that spontaneous recovery was equivalent in tetO-R(AB) and wild-type mice, consistent with the idea that the development of extinction within a session was facilitated by PKA inhibition. In the tetO-R(AB) mice, neuronal PKA was reduced during systems consolidation, the process by which storage of contextual memories become increasingly dependent on other brain regions such as the cortex (Frankland and Bontempi, 2005). However, our observations that transgenic mice and wild-type controls performed similarly during the beginning of long extinction trials (Fig. 5B) suggest that R(AB) expression did not interfere with systems consolidation or with retrieval of remote memories. Together, these experiments demonstrate that the inhibition of neuronal PKA in the adult fore-brain is sufficient to facilitate fear extinction and suggest that not only the fear responses associated with recent but also remote fear memories can be more rapidly extinguished by inhibition of PKA.
We examined the role of PKA in extinction of context-evoked fear using two distinct yet complementary transgenic approaches that allowed us to reduce PKA activity selectively in forebrain neurons. Both approaches revealed a facilitative effect of PKA inhibition on the development of extinction. Such an effect was observed both across extinction sessions with daily brief exposures (3 min) to the conditioning context in the absence of shock (Fig. 1 A), as well as within extinction sessions during daily long exposures (24 min) to the context in the absence of shock (Fig. 2, ,5B).5B). These findings suggest that PKA inhibition affects both the short-term development of extinction within a session as well as the retention of extinction across sessions, depending on the duration of the extinction session. Spontaneous recovery was observed in both cases, either the next day, which occurred with long extinction sessions (Figs. 2, ,5B),5B), or 21 d later, which occurred with short extinction sessions (Fig. 1 B). Our demonstrations of spontaneous recovery support the idea that the behavioral changes that occurred during extinction did not result from erasure of the original memory, but instead reflected new learning, distinct from the learning that occurred during the acquisition of fear conditioning (Lattal and Abel, 2004; Rescorla, 2004; Power et al., 2006). Indeed, the studies presented here provide the first genetic evidence that fear extinction is itself a learning process with its own molecular requirements.
In what brain regions does PKA activity act to modulate extinction? The transgenic expression of R(AB) in our experiments reduced PKA activity in the prefrontal cortex, hippocampus, and amygdala, three structures that are important in extinction. Although we cannot be sure which structure mediated these extinction effects, we do know that the CaMKIIα promoter drives expression in excitatory forebrain neurons (Mayford et al., 1996a), suggesting that inhibitory projections within or between these structures [such as between the medial prefrontal cortex (mPFC) and the amygdala] likely were not responsible for the effects of PKA inhibition on extinction. Further, although there also is evidence that stimulation of the mPFC can enhance both the short-term and long-term development of extinction, most studies generally show that the mPFC is most critical for the consolidation or long-term retention of the extinction memory (Milad and Quirk, 2002) (for review, see Quirk et al., 2006). The short-term enhancements in extinction that we observed within a long extinction session did not result in long-term enhancements in extinction, suggesting that if the mPFC was involved, its role was limited to the short-term development of extinction, which seems unlikely given the results from previous studies (Quirk et al., 2006). Studies of the hippocampus have shown that permanent lesions do not affect extinction per se (Wilson et al., 1995; Frohardt et al., 2000; Ji and Maren, 2005) and inactivation of the dorsal hippocampus before extinction training attenuates, but does not entirely block, the development of extinction, suggesting that the hippocampus is not essential for the acquisition of extinction (Corcoran et al., 2005). There are numerous studies showing that pharmacological manipulations of various signaling pathways in the amygdala before extinction training lead to facilitation or blockade of extinction, suggesting a critical role for this structure in the development of extinction (for review, see Barad et al., 2006). Thus, the amygdala may be a likely site of action for the effects of transgenic PKA inhibition on extinction that were observed in our experiments. Future experiments examining the role of PKA in the extinction of cue-induced freezing may provide a way to examine more selectively the role of PKA within the amygdala during extinction.
How does PKA act to facilitate extinction? There are two very general ways that extinction may be facilitated: some aspect of the original memory may be weakened, or some aspect of the extinction memory may be strengthened. Many studies have shown that extinction can occur through a temporary weakening of the original memory, either through a depression of the CS–US association, or through a depression of the representation of the CS or US itself (Pavlov, 1927; Rescorla and Cunningham, 1977; Robbins, 1990; reviewed in Rescorla, 2004; Schmajuk and Larrauri, 2006). At a theoretical level, the loss of conditioned behavior after an extinction session is often attributed to a deficit in the reconsolidation of the original memory. Behaviorally, enhancements in extinction and impairments in reconsolidation are functionally equivalent: both result in a loss of conditioned responding. However, one complication from our experiments in attributing the effects of PKA inhibition on extinction to deficits in reconsolidation is that short-term extinction was enhanced, suggesting that changes in performance occurred quickly. Thus, the time course of this effect suggests a rapid process, such as a within-session change in attention to the CS, which may occur in extinction (Schmajuk and Larrauri, 2006). Our observations of spontaneous recovery after PKA inhibition are consistent with many other studies of extinction showing that any effect on the original associative learning was temporary.
The alternative to some aspect of the original memory being weakened is that some aspect of the extinction memory was strengthened as a direct result of PKA inhibition. The idea that PKA inhibition enhanced new learning is counterintuitive because the effects of PKA inhibition on memory are often attributed to a failure to sufficiently activate transcription factors, leading to deficits in memory (Abel et al., 1997). However, PKA inhibition also can result in changes in phosphatase activation (Genoux et al., 2002; Mansuy, 2003), which may be critical for the development of extinction. One prevailing theory about the molecular mechanisms underlying learning and memory is that kinases and phosphatases oppose each other to regulate signaling pathways, synaptic efficacy, and learning and memory (Lisman, 1989). Generally, kinases are considered positive modulators of learning and memory whereas phosphatases are thought of as constraints for these processes (Genoux et al., 2002). However, it has been hypothesized recently that whereas excitatory responses to a conditioned stimulus (like those triggered by fear acquisition) may be orchestrated by kinases, inhibitory responses (like those triggered by fear extinction) may be supported by phosphatases (Myers and Davis, 2002). Evidence that extinction may be mediated by phosphatases came recently from work showing that the Ca2+-dependent phosphatase calcineurin is rapidly activated in the amygdala in mice after extinction training, and that extinction is blocked by pharmacological inhibition of calcineurin (Lin et al., 2003a, b). Given that PKA counteracts calcineurin and protein phosphatase 1 (Genoux et al., 2002; Mansuy, 2003), the facilitatory effect of PKA inhibition on extinction may be attributable to partial removal of an inhibitory constraint imposed by PKA on calcineurin signaling during extinction.
Consistent with this interpretation, transgenic mice overexpressing type 1 adenylyl cyclase with increased PKA activity levels in forebrain neurons, also exhibited slower extinction, indicating that increased PKA activity impaired fear extinction (Wang et al., 2004). Moreover, subchronic delivery of rolipram in rats has been shown to slow extinction of conditioned fear (Monti et al., 2006). Similarly, McNally et al. (2005) found that administration of the cAMP analog 8-Br-cAMP impaired extinction, although PKA activation by Sp-cAMPs had no effect. Additional indirect genetic evidence for this negative role of the cAMP–PKA pathway in extinction comes from cannabinoid receptor 1 (CB1) knockout mice, which showed impaired extinction (Marsicano et al., 2002). Because activation of CB1 receptors leads to adenylyl cyclase inhibition, it is possible that endocannabinoids may subserve extinction through inhibition of adenylyl cyclase.
These data together with our findings suggest that PKA, although required for fear memory formation, may be a molecular constraint for fear extinction, but the specific ways in which PKA may act on inhibitory processes during extinction remains to be determined. The basic demonstration in our experiments that the same genetic manipulations that impair initial memory formation may facilitate subsequent extinction is consistent with many other studies showing behavioral, systems, and molecular differences between initial learning and extinction (for review, see McNally et al., 2005; Bouton et al., 2006; Lattal et al., 2006). However, other studies have found a number of important similarities between initial learning and extinction, suggesting that a complete picture of extinction memory formation and consolidation will need to include multiple mechanisms. Understanding these mechanisms may help translate basic research to clinical settings, where extinction is a commonly used therapeutic intervention for psychiatric disorders involving fear (Bouton et al., 2001; Rothbaum and Schwartz, 2002; Richardson et al., 2004; Barad, 2005; Davis et al., 2006; Milad et al., 2006).
This work was supported by grants from the National Institutes of Health and the Human Frontiers Science Program (TA, C.I., E.R.K., K.M.L.). T.A. is a David and Lucile Packard Foundation Fellow. E.R.K. is also supported by grants from Howard Hughes Medical Institute. Wethank Tom Deuel, Kyle Lapidus, Alan Young, and Mark Barad for their participation in the early stages of this project. We thank Mark Mayford for the CaMKIIα-tTA mouse line B.