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Free Radic Biol Med. Author manuscript; available in PMC Sep 1, 2011.
Published in final edited form as:
PMCID: PMC2910817
NIHMSID: NIHMS211635
Antioxidant pre-treatment prevents omeprazole-induced toxicity in an in vitro model of infectious gastritis
Jonathan E. Kohler, Amy L. Blass, Jingjing Liu, Kaniza Tai, and David I. Soybel
Department of Surgery, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA
Correspondence: David I. Soybel, M.D., Department of Surgery, Brigham and Women’s Hospital, 75 Francis Street, Boston, MA 02115, Tel: +01 617 732 7930, FAX: +01 617 232 9576, dsoybel/at/partners.org
Omeprazole is a mainstay of therapy for gastroesophageal reflux disease (GERD) and gastritis, and is increasingly used as an over-the-counter remedy for dyspepsia. Omeprazole acts by selectively oxidizing thiol targets in the gastric proton pump, but it also appears to be toxic to the gastric mucosa. We hypothesized that omeprazole toxicity is due to non-specific oxidation of cell structures other than the proton pump, and tested the efficacy of antioxidants to prevent omeprazole-induced toxicity in isolated rabbit gastric glands. Toxicity was measured by uptake and converstion of calcein-AM, following three hours of exposure to omeprazole and a non-selective thiol-oxidant, monochloramine. Intracellular concentration of Zn2+ and the capacity to maintain luminal acidity were monitored using the fluorescent reporters fluozin-3 and Lysosensor DND-160, respectively. Both omeprazole and monochloramine caused marked reduction in cell viability. The toxicity of omeprazole was independent of monochloramine toxicity. The thiol reducing agent dithiothreitol protected gastric glands from injury. The oxidant scavenger Vitamin C also protected, and did not impair the anti-secretory effects of omeprazole. Thus, omeprazole toxicity appears to be oxidative and preventable with antioxidant therapy, including Vitamin C. Vitamin C may be a safe and efficacious addition to treatments requiring the use of PPIs.
Keywords: omeprazole, gastritis, Vitamin C, Helicobacter pylori, gastric mucosa, antioxidants
Omeprazole is a prototypical proton pump inhibitor (PPI), which covalently binds and inhibits the H+/K+ ATPase necessary for acidification of the tubulovesicle of the parietal cell. This prevents acid production and raises the pH of the stomach lumen, which has been shown to be an important component of therapy for the treatment of gastritis. Gastritis, defined as inflammation of the stomach mucosa, is a common clinical condition associated with local infection of the gastric mucosa. The predominant organism associated with gastritis is the urealytic bacterium Helicobacter pylori. Chronic gastritis has been estimated to be present in up to 37% of asymptomatic adults. Incidence increases with age and is almost always associated with H. pylori infection [1]. Omeprazole, in combination with antibiotics, effectively eradicates H. pylori infection. However, evidence suggests that omeprazole treatment may itself cause acute and chronic injury to the cells of the gastric gland. In experimental studies in rabbits, it has been shown that omeprazole leads to acute depletion of parietal cells, increased parietal cell turnover, and accumulation of chronic inflammatory cells [2]. This effect has been attributed to CCK-2 mediated proinflammatory effects of compensatory hypergastrinemia in response to decreased acid production [3]. In human studies, use of omeprazole alone as a treatment for H. pylori gastritis has also been demonstrated to increase mucosal inflammation. Prolonged inflammation, in turn, leads to destruction of the gastric glands and persistent hypergastrinemia, a condition known as atrophic gastritis. Atrophic gastritis resulting from omeprazole monotherapy in the setting of chronic H. pylori infection has been associated with increased risk of mucosal dysplasia and gastric cancers [46]. To our knowledge, direct toxicity of omeprazole to the cells of the gastric gland has not been previously reported.
In the setting of H. pylori gastritis, polymorphonuclear cells (PMNs) and macrophages are recruited to the gastric mucosa, where their antibacterial action includes the production and secretion of hypochlorous acid (HOCl). HOCl in turn can react with ammonia (NH3) produced by the metabolism of urea in H. pylori to form the potent thiol oxidant monochloramine (NH2Cl). A number of reports have suggested that NH2Cl can act as a significant agent of injury in gastric mucosa [710]. In addition, recent work in our laboratory has demonstrated that thiol oxidation with NH2Cl results in cell death associated with disruption of Ca2+ and Zn2+ homeostasis in the rabbit gastric gland [11, 12].
The mechanism of action of omeprazole also depends on thiol oxidation. Within the acidic environment of the parietal cell, omeprazole is converted into a thiophilic sulfenamide, which binds to a cysteine residue of the H+/K+ ATPase and oxidizes a thiol bond to render the pump inoperative. While this effect is generally believed to be specific to thiol groups of the H+/K+ ATPase, recent studies have suggested targets for oxidation that are apart from the gastric proton pump [1316]. These considerations suggest that, in the context of oxidative stress induced by gastritis, omeprazole may amplify injury to the gastric mucosa rather than accelerating healing.
In the studies reported here, we use a recently described in vitro assay [11] to evaluate the toxicity of a prototypical PPI, omeprazole, on gastric secretory glands. We demonstrate that omeprazole’s effects on the viability of the cells of the gastric gland may be through a previously underappreciated role as a thiol oxidant against multiple cellular targets. Our findings suggest that exposure to omeprazole amplifies cellular injury caused by NH2Cl, but that toxicity of both omeprazole and NH2Cl can be abrogated using concurrent antioxidant therapy.
Reagents and Solutions
Unless otherwise specified, reagents were obtained from Sigma (St. Louis, MO). Ringer’s Solution: NaCl 145mM, KH2PO4 2.5mM, MgSO4 1.0mM, CaCl2 1mM, HEPES 10mM, glucose 10mM, pH 7.4. Zinc and calcium-free Ringer’s solution: NaCl 145mM, KH2PO4 2.5mM, MgSO4 1.0mM, HEPES 10mM, glucose 10mM, 0.5 mM EGTA, pH 7.4. Omeprazole, TPEN (tetrakis-(2-pyridylmethyl) ethylenediamine), dithiothreitol (DTT) and ascorbic acid (VitC) were dissolved in Ringer’s directly. BAPTA-AM and Fluozin-3 AM (Invitrogen) were dissolved in DMSO. All solutions were checked for changes in pH and adjusted if necessary to baseline pH. Calculations of free and bound concentrations of Ca2+, Zn2+, TPEN, BAPTA and EGTA were performed using the internet-based WEBMAXSTANDARD program (http://www.stanford.edu/~cpatton/webmaxc/webmaxcS.htm).
Gland isolation
Anesthesia and euthanasia for New Zealand White rabbits were approved according to policies of Harvard Medical School. Gastric glands were harvested as described previously [12, 17]. Enrichment for parietal cells in gastric glands was previously confirmed [11] by immunofluorescent imaging using a mouse monoclonal antibody against the H+/K+-ATPase (ABCAM, no. ab2866) probed using a secondary donkey anti-mouse fluorescent antibody (Invitrogen, no. 10037). Other cell types present in the gastric mucosa are present in low numbers, with parietal cells accounting for a high percentage of the gland volume [18, 19]
Effects of omeprazole, monochloramine, intracellular zinc chelators, and antioxidants on cell viability
Glands were suspended in Ringer’s solutions with or without omeprazole (100µM) and monochloramine (0, 50, 100, and 200µM). After 2.5 hours of incubation at 37°C in sealed Eppendorf tubes, cell viability was assessed by monitoring uptake and intracellular conversion of non-fluorescent calcein-acetoxymethyl ester (calcein-AM) to fluorescent calcein by intracellular esterases, using a modification of previously reported methods [20, 21]. Calcein-AM was added to each tube (final concentration 8µM) for thirty minutes, after which glands were washed twice in Ringer’s solution and transferred to a 96-well plate for measurement of calcein fluorescence (Ex 485nm; Em 528nm; Syngery 2, BioTek Inc, Winooski, VT). To determine the effect of intracellular zinc on cell viability in the presence of monochloramine and omeprazole, the above conditions were recapitulated in the presence of the Zn2+ chelators BAPTA and TPEN. BAPTA is delivered across cell membranes in an acetoxymethyl ester form as BAPTA-AM, and is converted in the cytoplasm to BAPTA when, like calcein, the acetoxymethyl ester group is cleaved by intracellular esterases. For experiments including BAPTA-AM, glands were allowed to incubate with BAPTA-AM (10µM) for 45 minutes at room temperature before addition of omeprazole or monochloramine. For experiments including TPEN, 20µM was added simultaneously with omeprazole and monochloramine. To study the effects of antioxidants, glands were co-incubated with omeprazole, monochloramine and Vitamin C (1mM) or DTT (1mM).
Determination of intracellular Zn2+
Fluozin-3 acetoxymethyl ester (Fluozin-3 AM) was dissolved in DMSO then loaded (30 minutes at room temperature) into gastric glands suspended in DMEM containing 100µM cimetidine, pH 7.4, using dye concentrations between 4µM and 8µM. Subsequently, glands were rinsed several times in dye-free DMEM, mounted on glass coverslips, and transferred to the microscope stage, where they were superfused with Ringer’s solution at room temperature. Fluorescence was monitored concurrently in 6 to 10 individual parietal cells in each isolated gland using a real-time fluorescence imaging microscope (Nikon TE-2000U, Nikon, Inc, Melville, NY). Glands were excited at 488nm with emission measurement at 520 nm ± 15 nm. Digital images of glands were captured using a digital CCD camera (Hamamatsu ORCA-ER, Bridgewater, NJ). Images were processed using image processing software (Universal Imaging Corp., Downington, PA) to yield quantitative measurements of fluorescence intensity. Images were acquired every 10 seconds, using the minimum exposure time possible, to minimize photobleaching.
Determination of acidity in the tubulovesicle/lumen compartments of isolated gastric glands
Glands were loaded with 4µM Lysosensor DND-160 (Invitrogen) in DMEM for 15 minutes at room temperature. Following loading, solutions were exchanged for Ringer’s containing combinations of omeprazole (100 µM), vitamin C (1 mM) and DTT (1mM). In order to ensure that adequate substrate was available for mitochondrial function and optimal acid production, Ringer’s was supplemented with succinate (100 µM) and pyruvate (100µM). Excitation ratiometric measurements were performed using excitation wavelengths of 340 and 400nm, with emission filtering at 528nm [17, 22]. Results are presented as the ratio of relative fluorescence units (RFU): RFU(340nm)/RFU(400nm), with a higher ratio corresponding to a more alkaline pH [17].
Data Summary and Statistical Analysis
Fluorescence measurements were made at a single time point, and summarized as means ± SEM. For non-parametric comparisons of data normalized to control, Kruskal-Wallis one-way analysis of variance on ranks was performed, with multiple pairwise comparisons using Dunn’s method. For raw RFU measurements or comparisons of ratiometric values, the Holm-Sidak test for multiple comparisons was used, after testing for normal distribution of the data. Statistics were performed using standard statistical software (Sigma Stat, Version 3.5, Systat Software Inc., Port Richmond, FL).
Omeprazole is toxic, and enhances NH2Cl toxicity to gastric glands
We first tested the effect of omeprazole on the viability of rabbit gastric glands, both independently and in the presence of monochloramine (NH2Cl), which we have previously demonstrated to have toxic effects on gastric glands through a thiol oxidant mechanism [11]. For our assay of cell viability, we used the fluorescent dye calcein-AM. This dye is able to cross the cell membrane while associated with its acetoxymethyl (AM) group, but only becomes fluorescent after the –AM group is cleaved by functional intracellular esterases. In dead or dying cells, esterases are not present to perform this conversion, providing an effective loss-of-function assay for cell viability [23]. As shown in Figure 1, a 3-hour exposure to omeprazole (100µM) alone caused a 38% decrease in cell viability compared to control (± 6.4%, P < 0.01). This dose of omeprazole was chosen to produce a maximal inhibition of the H+/K+ ATPase in this in vitro model [2426]. When combined with monochloramine, omeprazole augmented its toxic effects at all but the highest dose used (200µM).
Figure 1
Figure 1
Toxicity of omeprazole and monochloramine (NH2Cl) as measured by calcein-AM uptake/conversion to fluorescent calcein
Omeprazole toxicity does not depend on dysregulation of intracellular Zn2+ levels
We have previously demonstrated that exposure to monochloramine leads to mobilization of intracellular Zn2+ stores, and that chelation of intracellular zinc improves cell viability when exposed to monochloramine [11]. In order to understand whether omeprazole also dysregulates intracellular Zn2+ homeostasis, we exposed gastric glands to omeprazole, using fluozin-3 to measure [Zn2+]i in individual parietal cells of isolated gastric glands [11, 22]. As shown in Figure 2, exposure to omeprazole did not itself lead to release of Zn2+ to the cytoplasm.
Figure 2
Figure 2
Representative recording of [Zn2+] in an individual gastric gland, during exposure to omeprazole
In order to further confirm the zinc-independent nature of omeprazole toxicity, we next pre-treated gastric glands with metal chelators before exposure to omeprazole. Shown in Figure 3 are the effects of the chelator BAPTA, which has affinity for Ca2+ (Kd~100nM) and for Zn2+ (~4nM) [27] levels released during exposure to NH2Cl but above expected resting levels of either cation [11, 12, 22]. By itself, BAPTA is not membrane-permeable; it is taken up as BAPTA-acetoxymethyl (AM) ester and activated by intracellular esterases. Glands were pretreated with low concentrations of BAPTAAM (10µM), to minimize over-loading of chelator in the cell [12, 28, 29]. When glands were exposed to omeprazole (100µM), in the presence or absence of NH2Cl, gland viability was not improved by pre-incubation with BAPTA-AM.
Figure 3
Figure 3
Effect of pretreatment with BAPTA-AM on omeprazole and NH2Cl toxicity
Our previous work [11] suggests that a significant component of NH2Cl-induced toxicity is related to the release of Zn2+. However, the results of studies summarized in Figure 3 suggest that if Zn2+ contributes to omeprazole-induced toxicity, it would be at concentrations below the range chelated by BAPTA. We therefore tested the effects of high-affinity zinc chelation, effectively eliminating free intracellular Zn2+, using the zinc chelator TPEN (tetrakis-(2-pyridylmethyl) ethylenediamine), which is membrane permeable and has a Kd for zinc in the femtomolar (10−14M) range [30]. Figure 4 shows that concurrent treatment of glands with 20µM TPEN prevented NH2Cl toxicity, while providing no protection against omeprazole toxicity. Interestingly, the degree of toxicity displayed by omeprazole continued to increase with increasing doses of NH2Cl, even when the effects of monochloramine alone were abrogated by TPEN pre-treatment. The findings summarized in Figures 3 and and44 confirm that while oxidant-induced accumulations of intracellular Ca2+ and Zn2+ may influence viability of gastric glands exposed to NH2Cl, they do not contribute directly to the toxicity of omeprazole.
Figure 4
Figure 4
Effect of TPEN on toxicities associated with omeprazole and NH2Cl
Effect of thiol reduction on omeprazole toxicity
To determine whether omeprazole exerts its toxic effects through a thiol oxidative pathway, we treated gastric glands with the selective thiol reducing agent dithiothreitol (DTT) concurrent with exposure to NH2Cl and omeprazole. As shown in Figure 5, preventing thiol oxidation with DTT completely protects glands from both omeprazole and NH2Cl toxicity. Results of these studies suggest that the cellular toxicity associated with both omeprazole and NH2Cl depends on thiol oxidation.
Figure 5
Figure 5
Thiol reducing agent DTT (dithiothreitol) prevents both monochloramine and DTT damage
Effect of Vitamin C on toxicity and therapeutic function of omeprazole
We next tested the effect of a commonly consumed nutritional supplement and known oxidant scavenger, ascorbic acid (Vitamin C), on omeprazole-induced toxicity. As shown in Figure 6, concurrent treatment with Vitamin C (1 mM) preserves gland viability in the presence of omeprazole, alone and in the presence of all tested concentrations of NH2Cl. These findings suggest that a non-thiol anti-oxidant may antagonize the toxic effects of omeprazole, both in isolation and in a cellular level model of gastritis-induced oxidative stress.
Figure 6
Figure 6
Oxidant scavenger Vitamin C prevents omeprazole toxicity
Because omeprazole depends on the thiol oxidation of the H+/K+ ATPase to exert its anti-secretory effects, the use of Vitamin C could potentially act as an inhibitor not just of the toxic effects of omeprazole, but of its desirable therapeutic effects. We therefore tested the effect of Vitamin C on the baseline acidity of the tubulovesicle/lumen compartment and its effect on the ability of omeprazole to inhibit the proton pump. Summarized in Figure 7 are fluorescence measurements of ratiometric excitation for the acid-responsive fluorophore Lysosensor DND-160 (340nm/400nm) [22]. Vitamin C alone does not alter pH of the tubulovesicle lumen compartment. As reported previously [17, 22], inhibition of the proton pump by omeprazole led to alkalization of the tubulovesicle/lumen compartment. As shown in the figure, concentrations of Vitamin C (1mM) that were protective against the toxic effects of omeprazole did not influence its ability to block acid secretion.
Figure 7
Figure 7
Effects of Vitamin C effect on acidity in the tubulovesicle/lumen compartment of isolated gastric glands
Omeprazole is the prototypical proton pump inhibitor, available over-the-counter and in inexpensive generic formulations. It is promoted as a therapy for a range of disease states, from mild heartburn to aggressive H. pylori gastritis, where it forms one component of the triple-agent therapy (clarithromycin, amoxicillin, and omeprazole) that is commonly used to eradicate H. pylori infection [6]. However, it is increasingly well-recognized that omeprazole may also contribute to gastric gland toxicity. In this report we use a recently described gland viability assay to show that omeprazole is toxic to cells of the rabbit gastric gland at physiologically relevant doses (Figure 1). We also demonstrate that this toxic effect occurs without the same disturbances to Zn2+ homeostasis that we have previously observed from a non-specific thiol oxidant, monochloramine (Figure 2) [11].
Like monochloramine, the toxic effects of omeprazole can be reversed using both a thiol reducer (DTT) and a generic antioxidant (Vitamin C) (Figures 5 & 6), suggesting that omeprazole’s effect on cell viability is through its action as a thiol oxidant. However, while chelating zinc with TPEN protects the gastric gland from monochloramine, chelation does not prevent injury by omeprazole (Figure 4). These data suggest that the two agents, while both acting as thiol oxidants, operate through different pathways to exert their toxic effects – monochloramine through a mechanism at least in part dependent on zinc-mediated toxicity, and omeprazole through a zinc-independent pathway.
The utility of Vitamin C to abrogate the toxicity of omeprazole may have clinical importance. Clinical studies have demonstrated that H. pylori eradication is improved when supplemented with Vitamin C [31] and that Vitamin C may decrease the total dose of antibiotic necessary for eradication [32, 33]. In addition, Vitamin C therapy has been shown to reduce histological and serological markers of inflammation in patients taking omeprazole as a monotherapy for H. pylori gastritis [34]. Because omeprazole is now available over-the-counter for treatment of dyspepsia, such potentially inappropriate monotherapy is increasingly common [35]. To our knowledge, this is the first report to identify a potential mechanism by which Vitamin C may provide benefit when co-administered with omeprazole in H. pylori gastritis, by protecting the cells of the gastric gland against the toxic effects of both monochloramine and omeprazole. Because our studies suggest that pre-treatment with Vitamin C does not significantly impair the antisecretory potency of omeprazole, it may be a valuable adjunct to existing triple therapy for H. pylori disease, or to monotherapy with omeprazole for non-infectious conditions.
In addition to potential direct effects on the cells of the gastric gland by Vitamin C, omeprazole users may benefit from Vitamin C supplementation for more indirect reasons. Inhibition of the proton pump has been shown to decrease active secretion of Vitamin C into the lumen of the stomach [36] and to decrease plasma Vitamin C [37]. Decreased availability of Vitamin C may have multiple deleterious effects. Luminal Vitamin C, in its active, ascorbic acid form, scavenges nitrites, converting them to nitric oxide and preventing their transformation into potentially mutagenic N-nitroso compounds [38, 39]. Vitamin C has also been demonstrated to increase absorption of non-heme iron [40]. At higher pH, such as in the stomach of patients taking PPIs, ascorbic acid is oxidized to inactive dehydroascorbic acid, meaning that decreased Vitamin C in the gastric lumen of patient using PPIs is further impaired by the elevated pH of the gastric lumen [37, 41]. Oxidation of ascorbic acid has also been shown in H. pylori infection, particularly in atrophic gastritis [4245]. This effect may be due to inflammatory mediators and to local pH elevation from ammonia produced by the bacterium [36].
Taken together, these data suggest that omeprazole acts through a previously unappreciated thiol oxidation pathway to cause a decrease in cell viability of the rabbit gastric gland in vitro. A large number of potential targets for thiol oxidation exist within the cells of the gastric gland, as a wide range of intracellular proteins are susceptible to thiol oxidation. However, these data suggest that differential thiol oxidation occurs between monochloramine (which appears to act through a zinc-dependent pathway) and omeprazole (which does not depend on zinc to exert its toxic effect). That both monochloramine and omeprazole toxicity can be reversed with Vitamin C provides a mechanistic explanation for previously observed benefits to Vitamin C co-administration in H. pylori gastritis treated with omeprazole. Further elucidation of the mechanism of action and the benefits of ascorbic acid in treating and preventing gastritis may improve patient outcomes in the future.
ACKNOWLEDGEMENTS
This work was supported by: American College of Surgeons Resident Research Fellowship and T32 DK007754 (JEK); R01 DK069929 (DIS); and Summer Student Research Fellowship from the American Physiological Society (KT).
LIST OF ABBREVIATIONS
BAPTA1,2-bis(0-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid
DTTdithiothreitol
HOClhypochlorous acid
GERDgastro-esophageal reflux disease
NH3ammonia
NH2Clmonochloramine
PMNpolymorphonuclear cell
PPIProton Pump Inhibitor
TPENtetrakis-(2-pyridylmethyl) ethylenediamine
VitCVitamin C/Ascorbic Acid

Footnotes
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