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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Nature. Author manuscript; available in PMC 2011 January 1.
Published in final edited form as:
PMCID: PMC2910425
NIHMSID: NIHMS212110

Structure of the Gating Ring from the Human High-conductance Ca2+-gated K+ Channel

Abstract

High-conductance Ca2+-gated K+ (BK) channels are essential for many biological processes such as smooth muscle contraction and neurotransmitter release1-4. This group of channels can be activated synergistically by both voltage and intracellular Ca2+, with the large C-terminal intracellular portion being responsible for Ca2+ sensing5-13. Here we present the crystal structure of the entire cytoplasmic region of the human BK channel in a Ca2+ free state. The structure reveals four intracellular subunits, each comprising two tandem RCK domains, assembled into a gating ring similar to that seen in the MthK channel14 and likely representing its physiological assembly. Three Ca2+ binding sites including the Ca2+ bowl are mapped onto the structure based on mutagenesis data. The Ca2+ bowl, located within the second RCK domain, forms an EF-hand like motif and is strategically positioned close to the assembly interface between two subunits. The other two Ca2+ (or Mg2+) binding sites, Asp367 and Glu374/Glu399, are located on the first RCK domain. The Asp367 site has high Ca2+ sensitivity and is positioned in the groove between the N- and C- terminal subdomains of RCK1, whereas the low affinity Mg2+-binding Glu374/Glu399 site is positioned on the upper plateau of the gating ring and close to the membrane. Our structure also contains the linker connecting the transmembrane and intracellular domains, allowing us to dock a voltage-gated K+ channel pore of known structure onto the gating ring with reasonable accuracy and generate a structural model for the full BK channel.

BK channels, also called MaxiK or Slo1, are a vital class of K+ channels found throughout the animal kingdom and expressed in a variety of cell types1-4. Several unique biophysical properties, including a large conductance and a gating mechanism involving both voltage and intracellular Ca2+, distinguish them from other K+ channels. The S1-S4 transmembrane region of BK channels forms a voltage sensing domain (VSD) as in other voltage-gated K+ channels, whereas the large C-terminal intracellular ligand binding part, accounting for two thirds of the full channel, is responsible for sensing Ca2+(5-13) as well as other intracellular stimuli15. The latter has been predicted to contain two RCK domains, first identified in an E.coli K+ channel and conserved in the majority of prokaryotic ligand-gated K+ channels14,16 as well as in the bacterial K+ uptake and efflux machinery (the KTN domain)17,18. While the first RCK domain of BK shares high sequence similarity with its prokaryotic counterparts, the less homologous second RCK domain is not as well defined. Even though the intracellular ligand gating properties of BK channels have been extensively studied, little is known about the structural details underlying their ligand gating mechanism beyond the proposed models based on MthK16,19,20 and a recent low resolution structure from electron cryomicroscopy21. The structure of the intracellular ligand binding domain presented here therefore provides essential structural information to guide future studies aimed at understanding how these physiologically essential channels integrate multiple cellular stimuli to modulate membrane excitability.

A human BK (hslo1) intracellular domain construct starting from Arg329, located right after the pore lining inner helix, to the C-terminal end (Supplementary Fig. 1) was chosen for structural characterization. Additionally, an extra 32-amino acid sequence from a mutant transcription factor GCN4 leucine zipper (GCN4_LI) known to form a 4-helical coiled-coil domain was inserted before the N-terminus of the BK intracellular domain. The protein was also treated with Lambda protein phosphatase before crystallization. Both modifications proved essential to obtaining good quality crystals. The crystals, which were of space group I422 and contained one subunit per asymmetric unit, revealed four crystallographically related subunits assembled as a tetramer with the molecular 4-fold coinciding with the crystallographic tetrad. The final model was refined to 3.1 Å with Rwork of 23.8 % and Rfree of 28.9 % (Methods and Table I).

Despite low sequence identity and large differences in sequence length between BK channels and prokaryotic RCK-regulated K+ channels, the intracellular portion of human BK contains two tandem RCK domains as predicted14 and its structure is strikingly similar to that of its prokaryotic counterpart, the MthK channel (Fig. 1 and Supplementary Fig. 1 & 2). For comparative purposes, secondary structure elements of each BK RCK domain are labeled following the same nomenclature used for its prokaryotic counterparts (Supplementary Fig. 1).

Figure 1
The BK intracellular subunit contains two RCK domains. The structure of the individual RCK domain is shown in a, RCK1 (yellow) and b,RCK2 (magenta). Only selected secondary structural elements are labeled for clarity. The arrow indicates the starting ...

Each RCK domain can be divided into three subdomains: an N-terminal Rossmann folded subdomain (from βA to βF) that forms the central core of the gating ring; the intermediate helix-crossover domain (αF-turn-αG) that interlocks the two RCK domains; and a C-terminal subdomain that interacts with its counterpart from a neighboring RCK domain within the same subunit (Fig. 1a&b and Supplementary Fig. 2). The two RCK domains form a bi-lobed architecture with extensive inter-RCK interactions at the helix cross-over and C-terminal subdomain, reminiscent of the RCK homodimer in MthK (Fig. 1c&d). Interestingly the deep cleft between the two RCK domains of MthK as well as other prokaryotic RCK proteins is occupied in the BK channel mainly by residues from the extended loops between βD and αD of both RCKs (Fig. 1c). Whereas this wide open cleft allows for large conformational changes at the flexible interface at its base in MthK upon ligand binding, its absence in BK raises the question whether similar conformational changes also occur. Further studies are needed to address this issue.

Four BK intracellular subunits assemble as a gating ring of eight RCK domains. The Rossmann folded subdomains from eight RCKs form the central core of the gating ring while the C-terminal subdomains form four protruded knobs at its periphery (Fig. 2a). The inter-subunit interactions occur at the assembly interface formed by helices αD and αE from each RCK domains in a head-to-tail manner; that is, RCK1 contacts RCK2 from the neighboring subunit. The protein-protein contacts at the assembly interface are mainly hydrophobic, involving residues Ile441, Met442, Ile445 on αD and His468 on αE of RCK1 and residues Ile821, Leu822, Leu825 and Phe890 on the equivalent helices of RCK2 from the neighboring subunit (Fig. 2b and Supplementary Fig. 3). The three residues on αD that form the core of the assembly interface are conserved as hydrophobic in most RCK domains14,16. Interestingly, αEs from both BK RCK domains appear to be 310 helices. Compared to MthK, the BK gating ring is more expanded, as expected for a protein of much larger size. The diagonal distance of the gating ring is about 82 Å, measured between the Cα atoms of Arg342 residues located at the outer rim of the ring (Fig. 2c). The BK gating ring also has a wide central hole with a width of about 20 Å, allowing ions to move freely between the ion conduction pore and the intracellular side. The crystals were obtained in the absence of Ca2+ and no bound divalent cations were observed in the structure. This led us to suggest that the BK gating ring structure shown here might represent a closed conformation.

Figure 2
Four BK intracellular subunits assemble into a gating ring. a, Stereo diagram of the gating ring structure viewed down the 4-fold axis from the extracellular side. Four long arrows indicate the position of inter-subunit assembly interfaces and define ...

While the BK intracellular domain is responsive to various chemical stimuli inside the cell, Ca2+ activation has so far been best characterized and will be the focus of the discussion here. Micromolar concentrations of Ca2+ can activate voltage-dependent gating of BK channels4,22,23. This high Ca2+ sensitivity can be partly attributed to a stretch of well-conserved aspartate residues within the second RCK domain, known as the Ca2+ bowl6,7. The Ca2+ bowl is located right after αE in the second RCK followed by a short helix (αEF), and is physically in close proximity to the assembly interface (Fig. 3a&b). Our structure was determined in the absence of Ca2+ and the stretch of acidic residues in the Ca2+ bowl does not form a well ordered structure. The short helix following the Ca2+ bowl is not seen on BK RCK1 and other RCK proteins, and was assigned as αEF since it is between αE and βF. Together with the Ca2+ bowl and αE, this helix forms a helix-loop-helix motif (αE-loop-αEF) very similar to Ca2+ binding EF-hand proteins such as Calmodulin24,25 (Fig. 3c), despite αEF being much shorter than the exiting helix (F helix) of most EF-hand proteins. It is likely that the disordered Ca2+ bowl structure represents an apo state and becomes ordered upon Ca2+ binding as commonly seen in EF-hand proteins which are also rich in acidic residues at the Ca2+ binding loop25. The strategic location of the Ca2+ bowl besides the inter-subunit assembly interface led us to postulate that Ca2+ binding triggers conformational changes at the assembly interface, changing the size of the gating ring and leading to channel activation.

Figure 3
Ca2+ binding sites in the BK intracellular subunit. a, A side view of the BK gating ring with the top surface facing the membrane. The front subunit is removed for clarity. The circled positions represent the three Ca2+ binding sites on one subunit, and ...

In addition to the Ca2+ bowl, recent studies have also identified at least two other independent Ca2+ binding sites within the first RCK domain8-12. One such site also has high Ca2+ sensitivity and involves an acidic residue, Asp367, located on the loop between αA and βB. In context of the gating ring, this site is positioned in the groove between the N- and C-terminal subdomains of RCK1, exposed to the open space between the membrane and the peripheral C-terminal subdomain (Fig. 3a). Figure 3d shows the chemical environment surrounding Asp367, comprising the side chains of His365, Met513, and Glu535. His365 has been shown to be involved in pH activation of BK and was proposed to interact electrostatically with Asp367 upon protonation26, mimicking the effect of Ca2+. The location of Met513 at this site explains the profound effect on Ca2+ sensitivity observed upon mutation9. Even though the side chain of Met513 may not directly bind Ca2+, it may play a key role in maintaining the local structural integrity of the Ca2+ binding site. The chemistry of the Asp367 ion binding site with a limited number of ligands does not seem suitable for high affinity Ca2+ binding. It is likely that residues from other parts of the channel may participate in Ca2+ binding. One possibility is residue Glu535 on the C-terminal subdomain of RCK1 whose side chain seems to be perfectly positioned to chelate Ca2+ together with Asp367, and may well be involved in Ca2+ binding, a hypothesis that warrants further investigation through mutagenesis.

The second Ca2+ binding site on RCK1 has lower affinity and has been suggested to be physiologically regulated by Mg2+(10,11,27). This low affinity site formed by Glu374 and Glu399 located on two neighboring β strands, βB and βC, respectively (Fig. 3e), is very close to the linker between the gating ring and the channel pore. The hydroxyl group of Ser337 on the linker is oriented towards the ion binding site and could also serve as a ligand for Ca2+ (or Mg2+). This site is positioned on the upper plateau of the gating ring near the membrane surface (Fig. 3a), suggesting that residues from the intracellular-facing part of the membrane spanning channel may participate in forming ion binding sites. Indeed, a recent study has pointed to an acidic residue from the large S0-S1 loop being important for Mg2+ binding27.

The protein construct used in our structural study contains the linker region thought to directly connect the gating ring to the pore lining inner helices of the transmembrane pore. This linker has an extended strand-like structure pointing towards the center of the gating ring (Fig. 2a & 4) and does not seem to form any specific non-covalent interactions with other parts of the protein. A stretched conformation likely allows the four linkers of a functional BK tetramer to more effectively couple gating ring conformational changes to pore opening and closing. Indeed, insertion and deletion mutations at the linker region have a profound effect on BK channel gating28.

Figure 4
Structure of the linker between the gating ring and the channel pore. a, Top view of the four linkers (shown in ball and stick representation) between the gating ring (grey surface representation) and the channel pore. b, An enlarged side view of the ...

The formation of a 4-helical coiled-coil by the attached GCN4_LI in effect mimics the pore-lining inner helices in constraining the linker. We believe it does not adversely affect the linker structure as we observe a similar orientation and extended strand like structure of the linker connecting the pore and transmembrane ligand binding domain in a recent structure of a bacterial K+ channel from Geobacter sulfurreducens, which contains 2-tandem RCK domains as in BK channels, solved recently by our group (unpublished results). Taken together, these data provide plausible evidence suggesting that the linker structure seen in the BK gating ring is physiologically relevant.

The presence of the linker between the BK gating ring and its channel pore allowed us to generate a full channel model by docking a voltage gated K+ channel onto the gating ring (Supplementary Fig. 4). The S1-S6 portion of the Kv1.2 structure29 was used as an approximate model for the BK transmembrane pore and was positioned onto the BK gating ring assembly with its C-terminal end of the S6 inner helix right at the N-terminus of the BK linker. Even though specific architectural features of BK and Kv channels may be different, we nevertheless believe this full channel model provides useful information about the relative positioning/orientation of the BK ligand binding domain with respect to the pore.

A 3 Å monomeric crystal structure of the human BK intracellular domain along with a terameric gating ring model based upon the 6 Å cytoplasmic subunit structure of the chicken Slo2 channel, a Na+-activated BK homolog, was published immediately preceding this paper by the Mackinnon group30. The overall architecture of the individual subunits and the deduced gating ring model match very well with the structure of our tetrameric gating ring assembly. Moreover, in the presence of 50 mM Ca2+, the authors suggest their structures represent the ligand-bound, open conformation compared to our ligand-free structure. Taken together, these related studies provide the first glimpse of two possible gating-ring conformations of BK channels.

Methods Summary

The intracellular fragment of the human Slo1 gene was subcloned into the pFastBac-HTa vector and expressed in SF9 insect cells using a baculovirus system. The construct contains an N-terminal His-tag, TEV protease cleavage site and an extra 32-amino acid stretch of sequence RMKQIEDKLEEILSKLYHIENELARIKKLLGE from the mutant transcription factor GCN4 leucine zipper (GCN4_LI) immediately before the N-terminus of the BK intracellular domain. Expressed protein was first purified on Co2+ affinity resin followed by overnight TEV cleavage at 4 °C to remove the His-tag. Protein was concentrated and then treated with Lambda protein phosphatase before further purification on a gel filtration column. Purified protein was concentrated to about 7 mg/ml and crystallized at 4 °C using the sitting drop vapor diffusion method by mixing equal volumes of concentrated protein and well solution containing 3.5 M sodium formate and 0.1 M Tris.HCl, pH 8.5. The crystals were of space group I422 with cell dimensions of a=b=134.1 Å, c=231.6 Å, α=β=γ=90°, and contained one subunit per asymmetric unit. The structure was determined by single wavelength anomalous dispersion (SAD) using selenomethionine substituted protein crystals. Model building was aided by the Se sites and conserved structure features of RCK domains. The final model was refined to 3.1 Å with Rwork of 23.8% and Rfree of 28.9%, and contained residues 330 to 616, 684 to 833, and 872 to 1059. The density for the N-terminal GCN4_LI coiled-coil is weak and is not modeled in the final structure.

Supplementary Material

Acknowledgments

We thank A. Alam and M. Derebe for manuscript preparation; A. Pico for discussion in the early stages of this study; and X. Zhang for help in structure determination. Use of the Advanced Photon Source (APS) was supported by the US Department of Energy, Office of Energy Research. We thank the beamline (23ID and 19ID) staff for assistance in data collection. This work was supported by Howard Hughes Medical Institute and by grants from the NIH/NIGMS (RO1 GM071621) and Welch Foundation.

Footnotes

Supplementary Information is linked to the online version of the paper at http://www.nature.com/nature.

Author Contributions. Y.W performed sample preparation and structure determination. Y.Y performed protein expression and purification. S.Y performed model building and refinement. Y.W and Y.J. designed the research, analyzed data, and prepared the manuscript.

Author information. The atomic coordinates and structure factors have been deposited in the Protein Data Bank under accession number 3NAF. Reprints and permissions information is available at npg.nature.com/reprintsandpermissions. The authors declare no competing financial interests. Correspondence and requests for materials should be addressed to Y.J. (youxing.jiang/at/utsouthwestern.edu).

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