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Three-dimensional visualisation and image analysis. (a) Three-dimensional visualisation in the MedINRIA ImageViewer software of a young (leaf 6, 8 days after initiation, left) and a mature Arabidopsis leaf (leaf 6, 19 days after initiation, right). (b) Three-dimensional structural model of a young (leaf 6, 10 days after initiation, left) and a mature Arabidopsis leaf (leaf 6, 22 days after initiation, right). (c) Image analysis flow diagram: ImageJ macros are indicated in blue (blue background-fully automated, white background-semi-automated) and R scripts in green.