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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Am Acad Child Adolesc Psychiatry. Author manuscript; available in PMC Apr 1, 2011.
Published in final edited form as:
PMCID: PMC2909696
NIHMSID: NIHMS177432

Suggestive linkage of the CBCL juvenile bipolar disorder phenotype to 1p21, 6p21 and 8q21

Abstract

Objective

Several studies have documented a profile of elevated scores on the Attention Problems, Aggressive Behavior and Anxious/Depressed scales of the Child Behavior Checklist (CBCL) in youth with bipolar disorder. The sum of these scales, referred to as the CBCL Juvenile Bipolar Disorder (JBD) phenotype, has modest diagnostic utility, and high scores are associated with severity of psychopathology and poor outcome. Recently, a genomewide linkage scan of this measure in ADHD sibling pairs revealed a region of suggestive linkage on chromosome 2q21. The current study aimed to further identify quantitative trait loci that influence the CBCL-JBD phenotype by using a dense and thus, arguably, more powerful set of single nucleotide polymorphism markers in a different ADHD sibling pair sample.

Method

Subjects were 765 individuals from 154 families with CBCL data enrolled in a linkage study of ADHD. Linkage analyses were completed using a multipoint maximum likelihood variance components approach implemented using the statistical program SOLAR.

Results

Heritability of the CBCL-JBD phenotype was estimated at .71. Although no regions of the genome surpassed empirically-derived criteria for significant linkage (p=0.000038), peaks on 1p21.1 (p=0.00037;LOD =2.76), 6p21.3 (p=0.00054;LOD =2.60) and 8q21.13 (p=0.00081;LOD =2.44) surpassed the threshold for suggestive linkage (p=.002). These regions have been highlighted in genomewide scans of bipolar disorder in adults, schizophrenia, autism and ADHD.

Conclusions

Findings raise the possibility that genes in these regions influence variation on the CBCL-JBD scale and the emotional and behavioral dysregulation associated with severe psychopathology.

Keywords: juvenile bipolar disorder, CBCL, genomewide linkage

Introduction

Whether to characterize youth with extreme emotional lability, aggression and behavioral dysregulation as having juvenile-onset bipolar disorder (BPD) has been a source of debate among child psychiatrists; however, there is no disagreement that understanding the etiology of such behavior is a priority for the field. Children and adolescents who carry the diagnosis of BPD share several, though not all, symptoms with the adult onset form of the condition 1 and are at risk for poor outcome, including suicide attempts 2 and hospitalization.3 Yet, questions remain about diagnostic boundaries, including whether youth who lack discrete episodes of euphoria/grandiosity should be considered part of the bipolar spectrum 4 and how to account for symptomatic overlap with ADHD.57 Although recommendations have been made to assist with differential diagnoses,8 and a practice parameter for the disorder has been published,9 the lack of consensus among experts renders quantitative behavior rating scales an important tool for research into the origins of these problems.

Of particular interest is a profile from the well-studied, parent-rated Child-Behavior Checklist 10 consisting of elevated scores on the Attention Problems, Aggressive Behavior and Anxious/Depressed scales. The profile was first noted by Biederman et al. 11 to occur in youth with BPD and to differ from scores of those without the condition, including children with ADHD who showed only an elevated Attention Problems Scale. Elevations on one or more of these three scales have been documented in youth with BPD in inpatient and outpatient settings and across different cultures,1217 and the profile of elevations is supported by meta-analysis.18 The sum of the three scales, referred to as the CBCL-Juvenile Bipolar Disorder (CBCL-JBD) phenotype, has shown utility for the prediction of interview-based diagnoses of BPD in some studies,19,20 including one large longitudinal analysis.21 However, data from other samples, particularly those in which there is a low base rate of BPD,22,23 have not supported the profile’s diagnostic utility. Nonetheless, all studies are in agreement that elevated scores characterize patients of significant clinical concern due to suicidality,2224 comorbid mood and disruptive behavior disorders,23 hospitalization, 21 severe symptomatology 25 and/or functional impairment.23,26

Recently, twin studies have indicated a substantial genetic contribution to the CBCL-JBD phenotype. In an ADHD-enriched Missouri birth cohort of twins, the heritability of the measure was estimated at .65 22 and an association was found with the gene for the dopamine transporter though not for the dopamine 4 receptor. In a large sample of Dutch twins at ages 7, 10 and 12, Hudziak et al. 27 estimated the phenotype’s heritability to be .6 – .7. Longitudinal data from the same sample indicated stability of CBCL-JBD scores, with 80% of the stability due to additive genetic effects.28 Althoff et al. 29 also found a highly heritable CBCL-JBD latent class in this sample associated with suicidal ideation. Together, these estimates suggest that the measure may offer a window into the heritable biological mechanisms underlying the emotional lability, aggression and behavioral dysregulation frequently found in bipolar youth.

To date, only one previous molecular genetic study has examined this profile using a genomewide approach. McGough and colleagues30 conducted a linkage scan of the CBCL-JBD phenotype in a sample of affected ADHD sibling pairs from 270 families using 423 polymorphic markers. Although no regions yielded signals that exceeded the threshold for significant linkage, a region of suggestive linkage was found on chromosome 2q23, with a multipoint maximum LOD = 2.5. As McGough et al. point out, this region had previously emerged as a genomewide significant epistatic locus in a linkage scan of BPD in adults published in 2007.31 Thus, McGough et al.’s data support continued investigation into the genetic bases of the CBCL-JBD scale to find risk loci for psychiatric symptomatology associated with pediatric-onset BPD.

In the current study, we aimed to follow-up and extend findings from McGough et al.30 using a more densely spaced and thus arguably more powerful set of 4885 SNP markers in a different sample of ADHD sibling pairs. Given the prior literature, we predicted that our analyses would identify quantitative trait loci (QTL) that influence scores on the CBCL-JBD phenotype.

METHOD

Sample Ascertainment

Data were from an affected sibling-pair linkage study of ADHD conducted at Massachusetts General Hospital (MGH).32 Study methods have been described previously.32,33 The original study included 1212 individuals from 271 families with at least one pair of full biological siblings meeting DSM-IV ADHD criteria or with one member of the pair meeting full and the other meeting subthreshold (2/3) criteria. Families were ascertained through the MGH Pediatric Psychopharmacology Program, local child psychiatry and pediatric practices, newspaper ads, an outpatient child psychiatry clinic at Boston Children’s Hospital and the University of Nebraska. Potential subjects were excluded if their nuclear family was unavailable, or if they were adopted, refused a blood sample or had major sensorimotor handicaps, active psychosis or suspected IQ < 70. No ethnic/racial groups were excluded, resulting in a sample that was 95% Caucasian, 4% African-American and 1% other. After procedures and risks were described, parents provided written informed consent for themselves and their children to participate. Children and adolescents provided written assent. The study was approved by the Partners/MGH Human Research Institutional Review Board.

Clinical Assessment

For youth ages 6 to 17, DSM-IV Axis I disorders, including ADHD and BPD, were assessed using the Schedule for Affective Disorders – Child Epidemiological Version (Kiddie-SADS-E), a widely used semi-structured diagnostic interview, with established psychometric properties.34 For all youth, psychiatric data were collected from the mother or primary caregiver. Youth 12 and older were also directly interviewed. Children younger than 12 were not interviewed because studies suggest limited reliability of their reports.3538 Subjects 18 and older received the Structured Clinical Interview for DSM- IV (SCID) 39 with a supplement from the K-SADS-E to assess ADHD and other childhood-onset disorders.

Interviewers had Master’s or Bachelor’s degrees in psychology or a related field and were trained to high levels of reliability with one another and clinicians. Raters were blind to recruitment status because they were conducting psychiatric interviews for other MGH studies. Best-estimate diagnoses were made after blind review of data by psychiatrists and psychologists.40 To combine discrepant parent-offspring reports, the most severe diagnosis was used, unless it was deemed unreliable.

IQ was estimated using the Wechsler Intelligence Scale for Children- Third Edition 41,42 for subjects 6 to 17, and the Wechsler Adult Intelligence Scale- Third Edition 41,42 for those 18 and older.

Mothers filled out a CBCL on each of their children. The CBCL is a well-validated questionnaire that assesses behavior problems and social competence in children aged 6 to 18 years, as reported by a parent or primary caregiver for the two months prior to the questionnaire’s administration. The measure was normed on over 2,300 non-referred children representative of the U.S. population. Questionnaires were returned by mail on 319 youth from 154 families who were included in the current analysis. Table 1 shows the breakdown of family configurations useful for linkage analysis based on available phenotypes and genetic data.

Table 1
Families included in analyses based on available genotype and Child Behavior Checklist data

A CBCL-JBD phenotype was constructed for youth with available data. Consistent with the literature, the score reflected the sum of the T scores from the Attention Problems, Aggressive Behavior and Anxious/Depressed Scales.

Genotyping

Genotyping was completed at the Center for Inherited Disease Research at Johns Hopkins. Markers were derived using Illumina’s Linkage IVb SNP-based marker panel based on its BeadArray technology on a BeadLab system. This system shows good power for mapping complex phenotypes, with a genomewide average information content of > 97.1%. The original panel consisted of 6,008 SNPs across all autosomes and sex chromosomes. Markers cover the genome with an average genetic distance of 0.64 cM and an average 482 Kb gap. The average marker heterozygosity is 0.43 in Caucasians, 0.38 in African Americans and 0.36 in Asians. Using Illumina’s Gentrain software to assess the quality of genotypes, only 28 SNPs were dropped for poor performance. DNA samples from eight individuals were dropped due to poor performance. The missing data rate for all samples in the original family analysis was 0.192%. The overall missing data rate after removing Mendelian inconsistencies was 0.56%. Duplicate concordance was excellent: based on 56 experimental samples genotyped in duplicate, the blinded duplicate error rate was 0.002%. Thus, overall the genotype data are high quality.

To avoid biases in the estimation of identity-by-descent probabilities, the dataset was pruned to remove correlated SNPs (r2 > 0.2), resulting in a final set of 4885 markers. Marker physical and genetic positions were obtained from the published CIDR map http://www.cidr.jhmi.edu/snp_marker.html.

DATA ANALYSIS

Analyses were conducted using SOLAR version 4.1.5.43 Heritability for the CBCL-JBD phenotype was determined by maximum-likelihood univariate variance component analysis, which decomposed phenotypic variance and covariance into components due to polygenic additive genetic variance and environmental factors not shared between relatives. Given our sample of reared together non-twin siblings, the genetic parameter, in actuality, represents the upper bound of heritability because it also includes variance due to environmental factors shared between relatives; nonetheless, it is useful for assessing the suitability of the CBCL-JBD phenotype for inclusion in linkage analyses and comparability to heritability estimates in the literature. Age and sex were included as covariates in the analyses, and the significance of models was determined by the likelihood-ratio chi-square test.

We performed a multipoint maximum-likelihood variance components linkage analyses for the CBCL-JBD measure, adjusted for age and sex. This approach compares the maximum-likelihood estimation of the CBCL-JBD phenotype decomposed into contributions from the QTL and residual genetic and environmental components with the model based on the null hypothesis of no influence of the QTL. Consistent with McGough, we included a model with no dominance effects because twin data support predominantly additive genetic contribution to the scale. The LOD score was then converted to empirical pointwise P-values (−log10P) through the analysis of 1,000 genome-scan datasets simulated under the null hypothesis of no linkage. Suggestive and significant levels of significance were calculated using the 1,000 simulated genome-scans based on recognized guidelines.44

RESULTS

Demographic features of the overall sample have been described.32,33 Youth with available CBCL data who were the focus of the current analyses (N =319) were 61% male with a mean age of 11.1 (SD=4.0) and an IQ in the average range (106.9;SD=15.0). Seventy-eight percent of youth met criteria for full DSM-IV ADHD (45% Combined, 28% Predominantly Inattentive and 6% Predominantly Hyperactive/Impulsive Types). Comorbid conditions were: oppositional defiant disorder (38.5%), conduct disorder (11.0%), major depressive disorder (14.4%), BPD (16.3%), generalized anxiety disorder (16.6%) and panic disorder (5.0%).

For purposes of comparison with other studies, means of the three T scores that were summarized to create the CBCL-JBD phenotype were: Attention Problems 62.6 (SD=10.9); Aggressive Behavior 59.4 (SD=10.4); and Anxious/Depressed 58.8 (SD=9.3).

Heritability of the CBCL-JBD phenotype was estimated at .71. No regions of the genome surpassed empirically-derived criteria for significant linkage (p=0.000038); however, regions on 1p21.1, (p=0.00037;LOD =2.76), 6p21.3 (p=0.00054;LOD =2.60) and 8q21.13 (p=0.00081;LOD =2.44) surpassed the empirically-derived threshold for suggestive linkage (p=.002; See Table 2 and Figure 1).

Figure 1
Results of the genomewide linkage scan of the Child Behavior Checklist (CBCL) juvenile bipolar phenotype. Values on the Y-axis reflect the −log10 empirical point-wise p-value, with lines indicating thresholds for significant (p=0.000038) and suggestive ...
Table 2
Detail of regions surpassing threshold for suggestive linkage.

Table 3 lists published genomewide studies of psychiatric disorders that have highlighted the regions of interest from our linkage scan. Notably, all three regions have been highlighted previously in studies of major psychiatric illnesses, including schizophrenia (1p21.1, 6p21.3 and 8q21.13), autism (1p21.1, 6p21.3 and 8q21.13) and bipolar disorder (6p21.3 and 8q21.13).

Table 3
Overlap of linkage peaks with regions of interest in genomewide analyses of psychiatric disorders

DISCUSSION

The CBCL-JBD phenotype is a parent-rated measure based on a profile of CBCL scale-elevations that have been replicated in bipolar youth. The three scales that contribute to the overall score (ie Attention Problems, Aggressive Behavior and Anxious/Depressed) contain items that reflect symptoms of cognitive, behavioral and emotional dysregulation. We conducted a genomewide linkage scan of this measure in 154 families ascertained through ADHD sibling pairs. Based on a panel of 4885 SNP markers, our scan identified three regions of the genome (1p21.1, 6p21.3 and 8q21.13) that surpassed empirically-defined criteria for suggestive linkage. These results extend support for the suitability of the CBCL-JBD phenotype for molecular genetic analyses and raise the possibility that QTL under these peaks influence psychiatric symptomatology in youth.

Prior to this analysis, only one study had examined the CBCL-JBD phenotype using a genomewide approach. McGough et al.30 found a region of suggestive linkage on 2q23, a location at which genomewide significance had been achieved in a two-dimensional linkage scan of adult bipolar disorder in 52 families. Our current study did not yield a linkage peak in this region. Inconsistent patterns of replication are often found in linkage studies of complex phenotypes, most likely due to genetic heterogeneity and the low power of linkage studies to find genes of small effect.45 In the current case, it is possible that this lack of convergence is due to differences in methods or sample characteristics, e.g. the UCLA study required that two parents be available for study, used a different diagnostic interview (i.e. KSADS-PL), had a lower rate of BPD and larger percentage of non-Caucasian subjects and used a microsatellite-based genotyping platform with less dense coverage.

Although not overlapping with McGough et al.30, the suggestive linkage peaks from the current scan include regions highlighted by prior genome scans of major psychiatric disorders. Like McGough et al., our data show convergence with risk loci identified by prior genomewide studies of BPD in adults. As shown in Table 3, our peaks on 6p21.3 and 8q21.13 were highlighted in a meta-analysis of genomewide expression studies of bipolar disorder. 46 Additionally, the region on 6p21 included a SNP ranked in the top 200 results from the STEP-UCL GWAS of BPD in adults47, and the region on 8q21 overlapped with secondary-level associations in the bipolar sample from the WTCCC GWAS (1 × 10 -5 <p value <1× 10-4).48

Moreover, all three regions overlap with genomewide scans of autism and schizophrenia. Our region on 1p21.1 overlapped with the primary findings in two previous linkage studies of schizophrenia and one of autism. For example, in 236 affected sibling pair families from Japan49, this region was the site of the primary (genomewide significant) linkage peak for schizophrenia in the study. In a smaller sample of 22 Canadian families with a heavy schizophrenia loading, this region harbored one of 18 peaks with a LOD score of >1.5 in.50 This region also included the peak marker in an initial genomewide scan of 90 multiplex autism families51 that showed allele sharing in an additional 49 families, though the combined sample fell short of genomewide significance.

Peaks on 6p21.3 and 8q21.13 also overlap with putative risk loci identified through genomewide scans of schizophrenia and autism. A meta-analysis of genomewide linkage scans from 200352 identified the 6p region as one of twelve (out of 120) bins considered strong schizophrenia candidate regions with P AvgRnk <.05 in weighted and unweighted analysis. Additionally, one of the top 25 SNPs from the first genomewide association scan of schizophrenia53 fell in this region, which also overlaps with 3 of 12 clusters of interest in the same study. The region identified on 8q21.13 overlapped with a secondary level association in the CATIE GWAS of schizophrenia.53 And, both the 6p and 8q regions were sites of autism-specific copy number variants.54

The current results are intriguing given data supporting a genetic overlap between schizophrenia and BPD,55 schizophrenia and autism 56 and growing evidence for a three-way genetic overlap between schizophrenia, BPD and autism.57 Although a small number of candidate genes that may influence this cluster of disorders have been explored in other regions (e.g. DISC1),58 more variants are expected to emerge to explain the familial and genetic overlap of these conditions. Our data raise the possibility that such variants lie within our highlighted regions and are indexed by the CBCL-JBD scale.

Determining precisely what construct is measured by the CBCL-JBD phenotype goes beyond the current study. Yet, convergence with genomewide scans of a range of conditions lends support to the hypothesis that the measure reflects a heritable trait relevant to a range of severe psychopathology rather than a specific measure of pediatric BPD per se. The items on the three scales that contribute to the CBCL-JBD profile reflect emotional and behavioral lability and distractability, i.e. items that index the capacity for self-regulation across a wide range of domains (i.e. cognitive, behavioral and affective). Further evidence for this conceptualization comes from Ayer and colleagues59 who found that the CBCL –JBD phenotype can be modeled as sharing a single latent trait with a different secondary CBCL scale purported to measure post-traumatic stress problems (PTSP). Like the CBCL-JBD phenotype, the PTSP scale is associated with suicidality and poor outcome and features a number of items overlapping with the CBCL-JBD that relate to self-regulation. Based on this analysis, the authors suggest both scales index a single dysregulatory syndrome.

The fact that the CBCL-JBD phenotype taps into a trait relevant to a range of psychiatric disorders may help to explain the profile’s lack of diagnostic specificity to juvenile-onset BPD in clinical studies.26 Yet, the empirical literature supports the relevance of the profile to the emotional and behavioral dysregulation that is characteristic of BPD in youth, thereby leaving open the possibility that genes that influence variation on the measure are relevant to this disorder. The profile of elevations that form the basis of the phenotype has been repeatedly replicated in affected children and adolescents.18 Faraone et al.19 also showed that the profile has excellent diagnostic efficiency for concurrent interview-based bipolar diagnoses in children and adolescents (AUC’s =.82 – .97) from an ADHD family study, and Diler et al.26 showed moderate diagnostic efficiency in retrospective ratings of individuals <12 years from a study of bipolar children (AUCs: .70–.77). A recent study21 further demonstrated good longitudinal prediction of BPD and associated features such as hospitalization, major depression and conduct disorder. Even in studies that have indicated poor diagnostic utility for the measure,2224,26 the association of high CBCL-JBD scores with suicidality and hospitalization2224,26 supports its relevance to a severe mood disorder.

Further work is needed to investigate susceptibility genes that may exist in these regions and their relationship to different forms of psychopathology. Given that genomewide association studies will have greater power and resolution than standard linkage scans to identify common variants of small effect, such studies provide the next logical step to follow-up these findings. Still, genomewide linkage scans such as this remain useful for identifying rare variants with strong effects in a small number of families or multiple mutations in the same gene that may differ across families. Thus, a lack of replication of the current results in a standard GWAS study of the CBCL-JBD phenotype would suggest that these other possibilities should be considered.

Such work is needed given how little is currently known about the genetic risk factors for mood disorders in children. As Mick and Faraone60 have recently reviewed, family studies and secondary analyses in adult bipolar linkage studies support the hypothesis that an early age of onset is associated with a greater genetic risk than adult-onset BPD. Nonetheless, no regions that have emerged in adult linkage studies of early-onset cases have been replicated or overlap with the current findings. A handful of candidate gene studies have found interesting associations with pediatric BPD for BDNF, SLC6A3 and GAD1, but contradictory findings and failures to replicate have not yielded definitive conclusions, and new candidates from the adult bipolar literature have yet to be tested in affected pediatric samples.

The current study should be considered in the context of its limitations. Our analyses, like those of McGough et al.30, were conducted in families originally ascertained through ADHD sibling pairs. Such a sample is relevant to the etiology of pediatric BPD given evidence from multiple samples of shared familial influences on ADHD and the disorder 61 and the high rate of ADHD in youth with bipolar diagnoses. Additionally, the heritability of the phenotype in the current sample was comparable to the heritability estimated from large population-based twin studies. Moreover, the QTL identified in these two studies do not overlap with loci identified through genomewide studies of ADHD, with the exception of the finding on 6p21.3 which included one of the top 25 SNPs from a genomewide association scan of ADHD.62 Thus, the current findings do not appear solely driven by ADHD. Still, further studies are needed to replicate and extend our findings in non-ADHD samples.

A second limitation is the low rate at which subjects in the sample returned the CBCL questionnaires. Our analysis is based on only 54% of the original families in our linkage study. Yet the comparability of subject characteristics, including IQ and rates of comorbid diagnoses in this analysis, to those in the original study suggests that the current sample is not skewed in terms of high functioning families.

A small number of additional factors are worthy of consideration. For example, we note that the CBCL-JBD phenotype was positively skewed. However, the evidence for linkage at each marker was estimated through simulations (ie. we generated empirical p-values rather than rely on asymptotic theory). Thus, any potential bias introduced by normality deviations would have been accounted for (i.e. no increase in false-positive rates would be expected because of the normality deviation). We further note that this is the third paper based on this same linkage sample, following published papers examining the ADHD phenotype specifically32 and a multivariate analysis of neurocognitive traits and ADHD symptoms33. Any single study can only realistically attempt to control its own study-wide error rate, and this and previous papers addressed specific, separate a priori hypotheses. Ultimately, though, issues of multiple testing should be acknowledged and our results require confirmation in independent samples. We also acknowledge that evidence for linkage to the identified regions is only suggestive. This finding, however, generates a very specific set of hypotheses (that loci regulating this phenotype are located in three defined chromosomal regions) that can be addressed by follow-up association studies.

Despite these concerns, our data raise the possibility that QTL on 1p21 6p21 and 8q21 contribute to variation on the CBCL-JBD phenotype. Further work is needed to confirm these findings, explore genes under these peaks and investigate the compelling hypothesis that risk variants in these regions contribute to severe, early-onset psychopathology and/or difficulties with self-regulation that overlap genetically with the shared risk for bipolar disorder, schizophrenia and autism.

Acknowledgments

This work was supported by R01HD37694 to Dr. Faraone and K08MH66072 to Dr. Doyle. Portions of this work were presented at the MGH-Ryan Licht Sang Pediatric Bipolar Disorder Foundation Conference on March 27, 2009 in Cambridge, MA.

Footnotes

Disclosure: Dr. Doyle has received research funding from the National Institute of Mental Health, Harvard Medical School and the Stanley Foundation. She received consulting fees from Pfizer Inc in 2007 and speaker¹s fees from Reed Education in 2008.

Dr. Biederman currently receives research support from the following sources: Alza, AstraZeneca, Bristol Myers Squibb, Eli Lilly and Co., Janssen Pharmaceuticals Inc., McNeil, Merck, Organon, Otsuka, Shire, National Institute of Mental Health (NIMH), and the National Institute of Child Health and Human Development (NICHD).

In 2009, Dr. Biederman received a speaker’s fee from the following sources: Fundacion Areces, Medice Pharmaceuticals, and the Spanish Child Psychiatry Association. In previous years, Dr. Biederman received research support, consultation fees, or speaker’s fees for/from the following additional sources: Abbott, AstraZeneca, Celltech, Cephalon, Eli Lilly and Co., Esai, Forest, Glaxo, Gliatech, Janssen, McNeil, the National Alliance for Research on Schizophrenia and Depression (NARSAD), National Institute on Drug Abuse (NIDA), New River, Novartis, Noven, Neurosearch, Pfizer, Pharmacia, The Prechter Foundation, Shire, The Stanley Foundation, UCB Pharma, Inc. and Wyeth. Dr. Faraone, in the past year, has received consulting fees and has been on Advisory Boards for Eli Lilly and Shire and has received research support from Eli Lilly, Pfizer, Shire and the National Institutes of Health. In previous years, he has received consulting fees or has been on Advisory Boards or has been a speaker for the following sources: Shire, McNeil, Janssen, Novartis, Pfizer and Eli Lilly. In previous years he has received research support from Eli Lilly, Shire, Pfizer and the National Institutes of Health. Drs. Ferreira and Smoller, and Ms Wong report no biomedical financial interests or potential conflicts of interest.

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