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Davide Schiavone, Sarah Dewilde and Francesco Vallania performed the experiments. Ferdinando Di Cunto analysed the data. James Turkson provided an essential reagent. Valeria Poli designed the experiment, provided funding and wrote the paper.
STAT3 (signal transducer and activator of transcription 3) is a transcription factor activated by cytokines, growth factors and oncogenes, whose activity is required for cell survival/proliferation of a wide variety of primary tumours and tumour cell lines. Prominent among its multiple effects on tumour cells is the stimulation of cell migration and metastasis, whose functional mechanisms are however not completely characterized. RhoU/Wrch1 (Wnt-responsive Cdc42 homologue) is an atypical Rho GTPase thought to be constitutively bound to GTP. RhoU was first identified as a Wnt-1-inducible mRNA and subsequently shown to act on the actin cytoskeleton by stimulating filopodia formation and stress fibre dissolution. It was in addition recently shown to localize to focal adhesions and to Src-induced podosomes and enhance cell migration. RhoU overexpression in mammary epithelial cells stimulates quiescent cells to re-enter the cell cycle and morphologically phenocopies Wnt-1-dependent transformation. In the present study we show that Wnt-1-mediated RhoU induction occurs at the transcriptional level. Moreover, we demonstrate that RhoU can also be induced by gp130 cytokines via STAT3, and we identify two functional STAT3-binding sites on the mouse RhoU promoter. RhoU induction by Wnt-1 is independent of β-catenin, but does not involve STAT3. Rather, it is mediated by the Wnt/planar cell polarity pathway through the activation of JNK (c-Jun N-terminal kinase). Both the so-called non-canonical Wnt pathway and STAT3 are therefore able to induce RhoU, which in turn may be involved in mediating their effects on cell migration.
STATs (signal transducers and activators of transcription) are transcription factors that play a major role in the signalling from cytokine receptors. STAT transcription factors become activated via tyrosine phosphorylation by receptor-associated JAK (Janus kinase) kinases , dimerize, concentrate into the nucleus and bind to STAT-responsive elements on the promoters of cytokine-induced genes. STAT3 was first isolated as the mediator of acute-phase gene induction in the liver in response to IL (interleukin)-6 and subsequently was identified as the main STAT transcription factor activated by all gp130 cytokines [2,3]. STAT3 is also activated by a wide variety of other cytokines and growth factors [e.g. leptin, IL-12, IL-17, IL-10, interferons, G-CSF (granulocyte colony-stimulating factor), EGF (epidermal growth factor) and PDGF (platelet-derived growth factor)] and by a number of oncogenic proteins such as c-Src, c-Abl, v-Sis, v-Fps, v-Ros, HER-2/neu and Met . A Stat3-null mutation in the mouse leads to early embryonic lethality, and conditional inactivation has revealed complex roles linked to inflammation, regeneration, proliferation, migration and energy homoeostasis .
Although STAT activity downstream of cytokines and growth factors is normally tightly controlled in both intensity and duration, STAT3 is found to be constitutively active in the vast majority of human and mouse tumours of both haematologic and solid origin, where its inhibition often leads to growth arrest and apoptotic cell death . STAT3 exerts its pro-oncogenic role both directly, inducing the expression of anti-apoptotic and cell-cycle genes in the tumoral cells, and indirectly, through down-modulation of tumour immune surveillance . STAT3 can also regulate cell movement, contributing to cytoskeleton reorganization and controlling cell adhesion properties, and is thought to play a role in tumour invasion and metastasis by inducing the expression of matrix metalloproteinases and promoting the EMT (epithelial–mesenchymal transition) (recently reviewed in ). In the zebrafish embryo, STAT3 activity is required for cell movements during gastrulation, a defect partially rescued by activation of the Wnt/PCP (planar cell polarity) signalling .
The evolutionarily conserved Wnt signal transduction pathway plays important roles in development and is strongly implicated in tumorigenesis. As for STAT3, aberrant Wnt signalling occurs in tumours of diverse origin and is likely to represent an important generic step in tumour development, contributing to functions as diverse as resistance to apoptosis, tissue invasion and metastasis, growth-factor-independent growth, evasion from immune response and sustained angiogenesis . Wnt proteins, acting through the frizzled family of seven-pass transmembrane receptors and through recently identified alternative receptors such as Ryk and Ror2 , can activate three different pathways: (i) the so-called canonical Wnt signalling pathway, leading to the stabilization and nuclear concentration of β-catenin and to the activation of LEF (lymphoid enhancer factor)/TCF (T-cell factor)-mediated transcription (this is the best characterized pathway mediating the proliferative effects of Wnt ligands); (ii) the Wnt/PCP pathway, involved in controlling cell polarity and movement, particularly during embryogenesis, and mediated by the activation of the JNK (c-Jun N-terminal kinase) pathway; and (iii) the Wnt/Ca2+ pathway, resulting in increased intracellular calcium and activation of PKC (protein kinase C).
Recently, a new Rho family member homologous with Cdc42 named Wrch1 (Wnt-regulated Cdc42 homologue)/RhoU has been isolated as a Wnt-1-responsive mRNA by means of subtraction cloning . RhoU is an atypical Rho GTPase with no detectable GTPase activity and high intrinsic guanine nucleotide exchange, and is thus likely to be constitutively GTP-bound [13,14]. Its regulation occurs both at the level of mRNA expression  and through release of the inhibitory effects of the N-terminal domain . RhoU acts on the actin cytoskeleton by stimulating the formation of filopodia, stimulates quiescent cells to re-enter the cell cycle and phenocopies Wnt-1-dependent morphological transformation of mouse mammary epithelial cells . Recently, RhoU was shown to localize to focal adhesions in HeLa cells and fibroblasts and to podosomes in osteoclasts and Src-expressing cells; its depletion causes an increase in focal adhesion numbers and inhibits cell migration [15,16].
In the present study we report that RhoU can also be induced by gp130 cytokines and that both its basal and cytokine-induced transcription in MEF (mouse embryonic fibroblast) cells requires STAT3, which binds to two STAT3-responsive elements on the mouse RhoU promoter. We also show that Wnt-1-mediated RhoU induction is regulated at the transcriptional level but is independent of STAT3, does not involve the canonical β-catenin-dependent pathway and requires JNK activity instead. Finally, we provide evidence for STAT3-mediated RhoU regulation also in human tumour or non-transformed epithelial cell lines.
Stat3+/+, Stat3−/−, Stat3Rev immortalized MEFs , HEK (human embronic kidney)-293, HEK-293-Wnt1, HeLa, PC3, DU145 and SKBR3 cells were grown in DMEM (Dulbecco's modified Eagle's medium; Gibco-BRL). T47D, SKOV3, MDAMB-231 and MDA-MB-468 were grown in RPMI 1360 (Gibco-BRL). Both media were supplemented with 10 % (v/v) heat-inactivated FCS (foetal calf serum; Gibco-BRL), 2 mM L-glutamine, 100 units/ml penicillin and 100 μg/ml streptomycin. MCF10A cells were grown in DMEM F12 (Gibco-BRL) supplemented with 5 % (v/v) horse serum (Gibco-BRL), 20 ng/ml EGF, 0.5 μg/ml hydrocortisone and 10 μg/ml insulin. All cell lines were maintained at 37°C in a 5 % CO2 atmosphere. The HEK-293-Wnt1 stable transfectants were generated by stable transfection with a Wnt-1 expression vector. Cells were treated with 20 ng/ml OSM (oncostatin M; R&D Systems) or with recombinant IL-6 (500 ng/ml) plus soluble receptor (250 ng/ml) [18,19] for different lengths of time. MEFs were treated with the following inhibitors: 50 μM SP600125 (Sigma–Aldrich), 10 μM Y-27632 (Sigma–Aldrich) and 100 μM myristoylated PKC peptide inhibitor (Promega) for the times indicated. DU145 and SKBR3 were treated with 50 mM S3I-201 (a STAT3 inhibitor) for the times indicated.
Total RNA was prepared with the PureLink Micro-to-Midi Total RNA Purification System (Invitrogen) according to the manufacturer's protocol. Samples were treated with DNase I Amp Grade (Invitrogen) before retrotranscription to eliminate genomic DNA contamination. To produce template cDNA, 125 ng of total RNA was reverse-transcribed using the RT High Capacity kit (Applied Biosystems) according to the manufacturer's protocol. qRT-PCR reactions were performed with the ABI Prism 7300 real-time PCR System (Applied Biosystems) using Platinum Quantitative PCR SuperMix-UDG with ROX (Invitrogen). The reactions were carried out in a total volume of 25 μlusing a TaqMan assay (Mm00505976_m1; Applied Biosystems) specific for murine RhoU. Human RhoU was detected by using the Universal Probe Library system (Roche) with the following primers and probe: forward primer, 5′-GACTCCAACTCTGTGACACTGC-3′; reverse primer, 5′-ATGAGGGGCTCACGACACT-3′; and probe #4. Results were analysed with the 2−ΔΔCt method using the 18S rRNA pre-developed TaqMan assay (Applied Biosystem) as an internal control.
EMSA probes/competitors consisted of double-stranded DNA oligonucleotides formed by a 9 bp long STAT3-binding site flanked by 3 bp on both sides and by a GATC protruding sequence on the 5′ end. Labelling and EMSAs were performed as described previously . Briefly, the HA-SIE (high-affinity sisinducible element) probe (1 pmol) was labelled using [32P]dCTP and Klenow and purified from free nucleotides on a Sephadex spin column. Nuclear extracts (4 μg) were pre-incubated on ice for 10 min in 80 mM KCl, 2 mM MgCl2, 2mM EDTA, 40 mM Hepes, 20 % (v/v) glycerol, 3 μg/reaction poly dI/dC, 5 mM DTT (dithiothreitol) and 1 μg/μl BSA. Competitor (1 μl), corresponding to concentration ratios 10-, 50- or 100-fold higher with respect to that of the labelled probe, was added and left to interact for 10 min on ice followed by incubation with the labelled probe (10000 c.p.m.) for 10 min at room temperature (25 °C). Samples were fractionated on a 5 % (v/v) polyacrylamide gel in 0.25 × TBE buffer (4.65 μg/μl Tris, 30 μg/μl boric acid and 60.5 μg/μl EDTA, pH 8.3) at room temperature. Gels were dried and autoradiographed. The oligonucleotide sequences used are indicated below: site1, upper 5′-GATCCATTTCCAAGACAGC-3′ and lower 5′-GATCGCTGTCTTGGAAATG-3′; site 2, upper 5′-GATCATATTAAAGGAATTC-3′ and lower 5′-GATCGAATTCCTTTAATAT-3′; site 3, upper 5′-GATCGGTTGCTGGGAATTG-3′ and lower 5′-GATCCAATTCCCAGCAACC-3′; site 4, upper 5′-GATCCTTTTCAGATAATAC-3′ and lower 5′-GATCGTATTATCTGAAAAG-3′; site 5, upper 5′-GATCAGGAGCCGGGAAAGG -3′ and lower 5′-GATCCCTTTCCCGGCTCCT-3′; site 6, upper 5′-GATCCGTTTCGAGGCGCCG-3′ and lower 5′-GATCCGGCGCCTCGAAACG-3′; HA-SIE, upper 5′-GGGCATTTCCCGTAAATCGT-3′ and lower 5′-CCCACGATTTACGGGAAATG-3′; and unrelated, upper 5′-GATCGGTTTCCGGACCAGC-3′ and lower 5′-GATCGCTGGTCCGGAAACC-3′.
Total protein extracts were obtained in 50 mM Tris/HCl (pH 7.4), 10 % (v/v) glycerol, 1 % (v/v) Nonidet P40, 150 mM NaCl, 2 mM EDTA, 2 mM DTT, 0.4 mM Na3VO4, 10 mM NaF, 0.5 mM PMSF and 40 μg/ml protease inhibitor cocktail (Sigma–Aldrich). Proteins were cleared by centrifugation (12000 g for 10 min at 4°C) and the concentration was measured using the Bradford assay (Bio-Rad). Samples were fractionated on SDS/PAGE and transferred on to PVDF membrane (Millipore) for immunoblotting. The following antibodies were used: anti-phospho-STAT3 (Tyr705; Cell Signaling Technology), total STAT3 (Santa Cruz Biotechnology), actin (Santa Cruz Biotechnology) and GAPDH (glyceraldehyde-3-phosphate dehydrogenase; Millipore).
RhoU promoter fragments were generated by PCR using a genomic BAC vector as a template. The following primers were used: −1765 forward (5′-ACTAGGTACCGAATTCAGGTGGCCCAAGGGC-3′); −1300 forward (5′-GGCCGGTACCGCATCTCTGTACTTCAGGTTGTCC-3′); −770 forward (5′-GGCCGGTACCCGCTAACAAAGGGTGGTATCCTGC-3′); −200 forward (5′-GGCCGGTACCGGTCTCGGATAGAAGAAGGAGCC-3′); and reverse (5′-TATCTCGAGGCCGCGAGACCAGCTGCC-3′). Amplified fragments were digested with KpnI/XhoI, cloned into pBlueScript (Stratagene), sequenced and subcloned into the promoter-less luciferase reporter vector pGL3basic (Promega). Mutant constructs were made by site-directed mutagenesis using the QuikChange® Mutagenesis Kit (Stratagene) according to the manufacturer's protocol. The following primers were used for mutagenesis (mutated bases are underlined): −1300 M2 sense (5′-GGGTATAAGTAATGCTAATATTAAAGCCCTTCGTTTTGACAGAATGGTTA-3′);−1300 M2 antisense (5′-TAACCATTCTGTCAAAACGAAGGGCTTTAATATTAGCATTACTTATACCC-3′); −1300 M3 sense (5′-CGTGAGTCACCATGTGGTTGCTGGCCCTTGAACTCAGGACC-3′); −1300 M3 antisense (5′-GGTCCTGAGTTCAAGGGCCAGCAACCACATGGTGACTCACG-3′); −1300 M5/−770 M5 sense (5′-GGAAGCTTGGGAAGGAGCCGGCCCAGGGGGGTGG-3′); −1300 M5/−770 M5 antisense (5′-CCACCCCCCTGGGCCGGCTCCTTCCCAAGCTTCC-3′); −1300 MA sense (5′-CAACAACATCTGGGACATAAACCAGGCTCAGTATTTAACTAGCTCTTGTC-3′); −1300 MA antisense (5′GACAAGAGCTAGTTAAATACTGAGCCTGGTTTATGTCCCAGATGTTGTTG-3′); −770 MB sense (5′-CCAAAGAGAGGGAGCGCTAAACCAGGGTGGTATCCTGCATC-3′); −770 MB antisense (5′-GATGCAGGATACCACCCTGGTTTAGCGCTCCCTCTCTTTGG-3′); −770 MC sense (5′-CAAAGGGTGGTATCCTGCATCCAAATGAGAATACCTGATTTTTAACAG-3′); −770 MC antisense (5′-CTGTTAAAAATCAGGTATTCTCATTTGGATGCAGGATACCACCCTTTG-3′). All mutant constructs were controlled by sequencing.
MEFs and HeLa cells were seeded in 24-well plates and transfected 24 h later with Lipofectamine™ reagent (Invitrogen). Each promoter construct was mixed at a 10:1 ratio with the pSEAP vector  as an internal control for transfection efficiency for a total amount of 0.4 μg of DNA and incubated with 5 μlof Lipofectamine™ according to the manufacturer's protocol. In cotransfection experiments, promoter constructs were mixed at a ratio of 5:5:1 (reporter/expression vector/pSEAP). At 24 h after transfection, the cells were treated with OSM or detached and re-seeded with an equal amount of Wnt1-expressing HEK-293 or wild-type control cells for co-culture experiments. At 48 h after transfection, the cells were washed twice with PBS and lysed in 50 μl of passive lysis buffer (Promega). Luciferase assays were performed using a luciferase assay system (Promega) according to the manufacturer's protocol. SEAP assays were performed using the phospha-light system (Applied Biosystems) according to the manufacturer's protocol. Luciferase and SEAP chemiluminescent activity were quantified using a Mithras LB940 multilabel reader (Berthold Technologies). SEAP values were used to normalize for differences caused by unequal transfection efficiency.
Stat3+/+ and Stat3−/− MEFs were treated or not with OSM as described above. ChIP assays were performed with the fast ChIP method  with some modifications. Briefly, cross-linking was performed with 1 % (v/v) formaldehyde for 10 min at room temperature. The reaction was quenched by the addition of glycine to a final concentration of 125 mM and incubation for 10 min at room temperature. Cells were lysed and sonicated at 4°Cfor six 10 s rounds followed by a 60 s pause, at an amplitude of 40 % (Ultrasonic Processor VCX-750, Sonics and Materials).
Immunoprecipitations were performed by incubating overnight at 4°C 1 ml of sheared chromatin with anti-STAT3 serum (R&D Systems, 5 μl), anti-acetyl-histone H3 (Upstate Cell Signaling Solutions, 2 μg) or negative control IgG (ChIP-IT Control Kit-Mouse; Active Motif, 2 μg). Primers for PCR amplification were designed using the program Primer3  to obtain an amplicon size of approx. 150 bp. PCR products were fractionated on a 2 % (w/v) agarose gel and stained with ethidium bromide. PCR reactions were performed using 5 μl of immunoprecipitated DNA using the following primers: RhoU site 3, forward 5′-GGCACCAGATCCCATTACAG-3′ and reverse 5′-TTTTGTGCTGCCCTTTCTCT-3′; RhoU site 5, forward 5′-ATCGCAAGGTCAGCTTTCAC-3′ and reverse 5′-CTTGCTTGGCCATTTACCAT-3′; Socs3 −65, forward 5′-GGAGAGACAGCGGTCGTAAG-3′ and reverse 5′-CACAGCCTTTCAGTGCAGAG-3′; and β-globin, forward 5′-CTCCCCCTCACTCTGTTCTG-3′ and reverse 5′-AGGAGGAGGGGAAGCTGATA-3′.
Gene expression profiling of Stat3+/+ or Stat3−/− MEFs either untreated or stimulated with different cytokines has led us to identify, among others, the atypical Rho GTPase RhoU/Wrch1 as a STAT3-dependent target of gp130 cytokine stimulation . Moreover, RhoU displayed a striking degree of conserved co-expression with STAT3 across hundreds of human and mouse microarray experiments , further suggesting a close functional correlation. Quantitative PCR analysis of RhoU mRNA levels showed indeed profoundly defective expression in Stat3−/− MEFs already under basal conditions (Figure 1a). In addition, whereas both IL-6 and OSM treatment induced RhoU mRNA in Stat3+/+ MEFs, they were completely uneffective in the absence of Stat3. As a result, RhoU expression levels were 4–5-fold lower in the Stat3−/− MEFs following cytokine stimulation. These defects are indeed STAT3-dependent since reintroducing Stat3 by stable transfection in the Stat3−/− MEFs (revertant MEFs) completely rescued both RhoU basal expression and cytokine inducibility (Figure 1a). RhoU mRNA induction in response to OSM stimulation was already appreciable 30 min after OSM treatment, peaked at 6 h and decreased to basal levels 24 h later (Figure 1b).
Since RhoU expression is induced by gp130 cytokines/STAT3, we searched the mouse RhoU promoter region for potential STAT3 regulatory elements. Bioinformatics analysis  identified six potential STAT3-binding sites (sites 1–6) that were assessed for in vitro binding capacity by EMSA competition assays (Figure 2a). Liver nuclear extracts from LPS-treated mice were used as a source for activated STAT3, with a labelled HASIE  double-stranded oligonucleotide as a probe. STAT3 binding to the HA-SIE probe was then competed with non-labelled double-stranded oligonucleotides based on the sequences of the six predicted STAT3-binding sites. Site 3 showed the greatest in vitro binding activity, with full competition at a ratio of 100:1 between non-labelled competitor and labelled probe, and partial competition at the ratios of 50:1 and 10:1. Sites 5 and 2 were also able to compete, albeit with lower efficiency/affinity, whereas sites 1, 4 and 6 did not show any significant competing activity. Self-competition with a unlabelled HA-SIE oligonucleotide or competition with an unrelated sequence were used as positive and negative controls respectively.
In order to analyse RhoU promoter activity, mouse RhoU promoter deletion fragments were fused to a luciferase reporter and transiently transfected in Stat3+/+ and Stat3−/− MEFs (Figure 2b). Transfected cells were either stimulated with OSM or left untreated for 24 h before measuring luciferase activity. In keeping with the mRNA expression results, transcriptional activity of all promoter fragments was drastically reduced in Stat3-deficient MEFs as compared with their wild-type controls already under basal conditions. In addition, the constructs −1765, −1300 and −770 were induced by OSM treatment in the Stat3+/+ MEFs, confirming that cytokine-dependent RhoU mRNA induction occurs transcriptionally. In contrast, OSM-dependent induction of all constructs was completely defective in the Stat3-deficient cells. Both basal and OSM-stimulated activity of RhoU promoter constructs were comparable with those of the mouse Socs3 promoter , a well-recognized STAT3 target. RhoU −200, the shortest promoter fragment tested, displayed reduced activity in both Stat3−/− and Stat3+/+ MEFs and was unresponsive to OSM treatment, suggesting that the region(s) required for OSM induction must be located upstream of the −200 position. Interestingly, and only in the presence of Stat3, both basal transcription levels and OSM inducibility increased with the progressive shortening of the promoter up to position −770, before dropping at −200. This might suggest the presence of repressor element(s) in the distal portions of the promoter. To assess the functional contribution of the tested STAT3-binding sites (see Figure 2a), sites 2, 3 and 5 were mutagenized in the context of the RhoU −1300 construct and the transcriptional activity of the resultant RhoU −1300 M2, M3 or M5 constructs tested in wild-type MEFs (Figure 2c). The site 2 mutation did not have any effect on either basal or OSM-induced expression, suggesting that this site is not involved in STAT3-mediated regulation. Indeed, site 2 was the one with the lowest in vitro binding activity (Figure 2a). Disruption of sites 3 or 5 significantly decreased transcriptional activation in response to OSM stimulation, and the compound double mutation (−1300 M3 + M5) completely abolished promoter inducibility and decreased its basal activity (Figure 2c). Thus both STAT3-binding sites 3 and 5 co-operate to confer full promoter activation in response to OSM stimulation. On the other hand, site 5 also plays a prominent role to confer OSM inducibility to the RhoU −770 promoter construct, since its mutation in this context (−770 M5) almost completely abolished OSM inducibility (Figure 2c). Site 6 did not appear to contribute to this residual induction since neither a site 6 single mutation nor a compound site 5/site 6 double mutation were able to abolish it (−770 M6 and M5+M5 mutants; Figure 2c).
To further analyse the in vivo functionality of sites 3 and 5 we assessed STAT3 in vivo occupancy by ChIP. ChIP assays were performed with chromatin samples prepared from Stat3+/+ and Stat3−/− MEFs, either untreated or stimulated with OSM for 30 min. With respect to the negative IgG controls, both sites showed weak STAT3 binding already in untreated cells (Figure 2d). Similar to what was observed for the well-characterized SOCS3 −65 STAT3-responsive element , STAT3 binding to both site 3 and site 5 was strongly induced by OSM, confirming their in vivo functional role in mediating RhoU promoter activity. Importantly, no STAT3 binding was ever detected with chromatin from Stat3−/− MEFs, thus confirming the specificity of the ChIP assay used in the present study.
Since RhoU was initially isolated as a Wnt-1-regulated gene, but its transcriptional regulation was never characterized, we decided to investigate the mechanisms involved in RhoU up-regulation downstream of Wnt-1 and the potential co-operation with the STAT3 pathway. Thus RhoU mRNA expression levels were measured in Stat3+/+ and Stat3−/− MEFs stimulated with OSM and Wnt-1 either alone or in combination (Figure 3a). Wnt-1 stimulation was obtained by co-culturing MEFs with HEK-293 cells stably expressing Wnt-1, whereas control cells were co-cultured with wild-type HEK-293 cells. In keeping with previous observations, RhoU mRNA expression was dependent on STAT3 already under basal conditions and failed to be induced by OSM stimulation in Stat3−/− cells. In contrast, Wnt-1 stimulation could similarly induce RhoU mRNA in both Stat3+/+ and Stat3−/− MEFs. Likewise, Wnt-1 could induce RhoU promoter transcription at similar levels in the presence or absence of Stat3 (Figure 3b). These results suggest that Wnt-1-mediated RhoU induction occurs at the transcriptional level and is independent of STAT3. This was further supported by the observation that the RhoU −770 M5 construct, which has lost OSM inducibility, is still fully responsive to Wnt-1 stimulation (Figure 4a). Thus both the Wnt-1 and the gp130/STAT3 pathways can activate transcription of the RhoU promoter, but they act through independent mechanisms. Of note, RhoU induction could not be obtained using the supernatants of the Wnt-1-expressing HEK-293 cells (results not shown), suggesting that specific stimulation by membrane-bound Wnt-1 and not secretion of soluble mediators by the Wnt-1-expressing cells is involved.
Similar to what was observed for OSM, Wnt-1-mediated induction progressively increased with promoter shortening, suggesting that the inhibitory sequence(s) already postulated in the case of OSM induction may be also active in the context of Wnt-1 stimulation (Figure 3c). As in the case of OSM responsiveness, the RhoU −200 construct was not anymore able to respond to Wnt-1 stimulation, indicating that the Wnt-1-responsive element(s) must lie in the region between positions −770 and −200. The reporter vector Super8X-TOPFlash and its negative control Super8X-FOPFlash  were used in all co-culture experiments to monitor Wnt pathway activation (results not shown).
The molecular mechanisms leading to RhoU gene activation downstream of Wnt-1 are currently unknown. Among the many signalling events activated by Wnt ligands, the canonical Wnt/β-catenin pathway which leads to the up-regulation of several target genes mediated by the LEF/TCF transcription factor is the best characterized [28,29]. Mouse RhoU promoter analysis using the TRANSFAC open-source program  identified three consensus sequences for LEF/TCF (sites A, B, C; Figure 2b). However, none of the three sequences appears to play a role in RhoU promoter activity, as shown by transient transfection assays of the relative promoter mutants −1300 MA, 770 MB or −770 MC (Figure 4a). Moreover, downstream activation of the Wnt-1/β-catenin pathway by expression of a stabilized mutant form of β-catenin did not have any effect on RhoU promoter transcription, whereas it strongly activated the TOPFlash control construct (Figure 4b). Thus the canonical Wnt/β-catenin pathway does not appear to be involved in mediating RhoU transcriptional induction.
Besides the canonical β-catenin pathway, Wnt ligands are known to activate the Wnt/Ca2+ and the PCP non-canonical pathways (as illustrated in Figure 7). In order to assess their potential involvement in RhoU induction by Wnt-1, we have used available specific chemical inhibitors to interfere with the activities of the main mediators involved.
First, RhoU mRNA levels were measured by qRT-PCR in wild-type MEFs treated or not with specific inhibitors for PKC (Wnt/Ca2+ pathway), ROCK (Rho-associated kinase) and JNK (PCP pathway) in the presence or absence of Wnt-1 stimulation (Figure 5a). Wnt-1-mediated RhoU mRNA induction was unaffected by PKC and ROCK inhibitors, whereas it was completely abolished by inhibiting JNK activity (Figure 5a). A similar effect was also observed at the level of RhoU promoter activation, since Wnt-1-mediated induction of the −770 promoter construct was strongly reduced in the presence of the JNK inhibitor (Figure 5b).
Thus RhoU transcriptional activation by Wnt-1 appears to be mainly mediated by the non-canonical PCP pathway, with a central role played by the MAPK (mitogen-activated protein kinase) JNK. Since the activation of JNK downstream of Wnt is known to be mediated by the small GTPases Cdc42 and Rac (see Figure 7), we performed co-transfection experiments of the RhoU promoter with constitutively active Rac1 and Cdc42 mutants (Figure 5c). Both Rac1-Val12 and Cdc42-Val12, but not the control RhoA-Val14, were able to significantly activate the RhoU promoter and this induction was abolished by JNK inhibition, strengthening the idea that Wnt-1 can activate the PCP pathway leading to the induction of the RhoU promoter through Rac1 and Cdc42-mediated JNK activation.
In order to extend our results to more biologically relevant experimental systems, we analysed RHOU mRNA expression and its correlations with the gp130/STAT3 pathway in human transformed and non-transformed epithelial cell lines. OSM treatment led to RHOU mRNA up-regulation also in HeLa cells, albeit with different kinetics as compared with MEFs (Supplementary Figure S1a at http://www.BiochemJ.org/bj/421/bj4210283add.htm). In the same cells, additionally, both OSM and Wnt-1 stimulation were able to induce RHOU promoter activity (fragment −770) to a similar extent as observed in MEFs (Supplementary Figure S1b and S1c).
Since RhoU is thought to mediate Wnt-1 oncogenic properties and might therefore play a similar role downstream of STAT3, we decided to correlate STAT3 activation and RHOU expression levels in a panel of human tumour cell lines well-characterized for STAT3 constitutive activation. RHOU expression levels (Figure 6c) were always elevated in cell lines with high (DU145, SKOV3, MDA-MB-231 and MDA-MB-468) or moderate (SKBR3) levels of constitutively active STAT3 (Figures 6a and 6b), all of which, with the exception of SKOV3 cells, have been reported to be addicted to STAT3 activity for survival/proliferation [31–33]. Conversely, cell lines with no detectable phosphorylated STAT3 showed variable RHOU mRNA levels (Figures 6a–6c). In particular, RHOU mRNA was almost undetectable in the breast cancer cells T47D, whereas its levels were comparable with those of the other cell lines in the prostate cancer PC3 cells. Of note, these are known to display constitutive Wnt-1 expression, which may be responsible for RhoU up-regulation in the absence of STAT3 activity .
In order to assess the functional correlation between STAT3 activity and RHOU expression, DU145 and SKBR3 cells were then treated with the specific STAT3 inhibitor S3I-201 . Both phospho- and total STAT3 levels were drastically reduced in both cell lines after S3I-201 treatment (Figure 6d), correlating with a dramatic decrease of RHOU mRNA levels as determined by qRT-PCR (Figure 6e; 78% and 93% reduction at 24 h in DU145 and SKBR3 cells respectively).
Finally, IL-6 stimulation triggered STAT3 phosphorylation correlating with a strong up-regulation of RHOU mRNA expression in MCF10A cells, a non-transformed human mammary cell line displaying almost undetectable levels of STAT3 phosphorylation under basal conditions (Figures 6f and 6g).
The atypical Rho GTPase RhoU was first identified as a Wnt-1-responsive gene by subtractive cloning . RhoU overexpression can trigger cellular transformation and phenocopies the effects of Wnt-1 in mammary epithelial cells, suggesting a potential role in Wnt-mediated tumorigenesis . Rather than being regulated by GDP/GTP exchange like most Rho GTPases, RhoU activity can be modulated by acting on its N-terminal domain, which exerts a partial inhibitory effect on the RhoU ability to activate PAK (p21-activated kinase) and to cause cellular transformation . This inhibition can be relieved by interaction of the PxxP motif in the N-terminal domain with SH3-containing protein adaptors such as Grb2 and Nckβ, suggesting functional interactions with receptor tyrosine kinase-activated pathways. Moreover, unlike most Rho GTPases that are constitutively expressed, RhoU expression is tightly controlled [35,36]. In addition to being induced by Wnt-1, RhoU mRNA has been recently shown to accumulate in osteoclasts in response to RANKL (receptor activator for nuclear factor κB ligand)-induced differentiation . However, the molecular mechanisms for either induction have not been characterized. In the present study, we demonstrate for the first time that RhoU induction by Wnt-1 occurs at the transcriptional level. Moreover, we show that RhoU is also a prominent target for STAT3-mediated transcription downstream of the gp130 cytokines IL-6 and OSM (Figure 7). Intriguingly, although both in vivo functional STAT3-binding sites 3 and 5 are required to confer full OSM inducibility to the −1300 RhoU promoter construct, site 5 alone is sufficient in the context of the shorter −770 promoter fragment, which lacks site 3. Both sites might indeed be required both in vivo and in the longer promoter construct to overcome the observed inhibitory effect of sequence(s) located between the −1300 and −770 positions. Interestingly, STAT3 was required not only for RhoU cytokine-mediated induction, but also for its basal expression, and both functionally tested STAT3-binding sites, site 3 and site 5, displayed a low level of constitutive binding following ChIP analysis. Constitutive STAT3 binding to a subset of sites was recently shown in NIH 3T3 cells , although a correlation with basal gene expression was not tested. Low amounts of Tyr705-phosphorylated STAT3 are present in growing MEFs and could be responsible for the constitutive binding observed. Alternatively, non-canonical mechanisms recruiting unphosphorylated STAT3 may be involved. At any rate, the ability of STAT3 to transcriptionally regulate a subset of target genes under basal conditions may have important implications for its physiology.
Both Wnt ligands and STAT3 play surprisingly similar multiple roles in tumorigenesis. In particular, both are involved in stimulating cell movements in tumoral cells as well as during development [8,39]. RhoU is known to act on the actin cytoskeleton, triggering filopodia formation and stress fibre dissolution. In addition, RhoU was recently shown to localize to focal adhesions and podosomes and to regulate their genesis promoting cell migration [15,16]. Intriguingly, STAT3 was also shown to localize to focal adhesions in HeLa and ovary tumour cells, where it interacted with activated focal adhesion kinase and paxillin and promoted cell migration , and we could demonstrate that activation of the gp130/STAT3 pathway induces both RhoU mRNA and promoter activity also in HeLa cells. Thus RhoU transcriptional induction might be one of the mechanisms through which STAT3 acts on focal adhesions and ultimately triggers cell migration. Indeed, the same Stat3−/− MEFs used in the present study, exhibiting low basal RhoU levels, also display impaired migration in a wound healing assay . In addition, the convergence of both Wnt-1 and STAT3 signalling on RhoU may represent a point of cross-talk between the two pathways, which has been proposed in several systems. STAT3 expression was induced by the Wnt/β-catenin pathway in oesophageal cancer cells and in embryonic stem cells [42,43]. STAT3 was also proposed to act upstream of the Wnt/PCP pathway during the gastrulation of zebrafish embryos  and to promote β-catenin nuclear accumulation in colorectal cancer cells . Moreover, the negative regulator of Wnt signalling Duplin was recently shown to also suppress STAT3 activity . Indeed, both STAT3 and Wnt-1 aberrant activation are often observed in the same tumours, suggesting that the two pathways can co-operate in different ways depending on the cellular context.
However, the convergence of the Wnt-1 and gp130/STAT3 pathways on RhoU induction must involve distinct mechanisms, since STAT3 is not required for Wnt-1-mediated induction of the RhoU promoter. In addition, as previously suggested by Tao et al. , RhoU induction by Wnt-1 appears also to occur independently of β-catenin, suggesting the involvement of one of the non-canonical pathways. We could identify this pathway with the Wnt/PCP pathway, since Wnt-1-mediated RhoU induction is abolished by an inhibitor of JNK activity. Interestingly, RhoU itself was reported to be able to activate JNK , suggesting the existence of a positive-feedback loop whereby activated RhoU may enhance its own transcription by means of JNK activation. Supporting and extending this observation, we could demonstrate that both constitutively active Rac1 and Cdc42, which lie upstream of JNK in the Wnt/PCP pathway, can activate RhoU transcription in a JNK-dependent way. Thus, at least in MEFs, Wnt-1 can activate both the canonical β-catenin pathway and the non-canonical PCP pathway at the same time, as shown by the simultaneous activation of RhoU and TOPFlash in our system. It is intriguing that the Wnt/PCP pathway is best characterized as regulating cell movements during embryonic gastrulation and that the main functions of RhoU appear to be linked to focal adhesions and cell migration.
At present, the transcription factors mediating the effects of the PCP pathway are unknown, and our system may provide a relatively straightforward way to identify at least some of them. However, bioinformatic analysis of the RhoU promoter region involved in Wnt-1 responsiveness did not disclose any obvious candidate, and co-transfection experiments with transcription factors potentially activated by JNK such as c-fos, c-jun and CREB (cAMP-response-element-binding protein) did not modify promoter activity (D. Schiavone and V. Poli, unpublished work). More detailed promoter analysis will therefore be required in order to identify the PCP-activated transcription factors. The identification of RhoU as a common transcriptional target of both STAT3 and Wnt-1, together with the known roles of both pathways in stimulating cell migration in normal and tumoral cells, suggests the intriguing idea that RhoU may represent a common mediator of STAT3 and Wnt effects on cell motility under both physiological and pathological conditions, being potentially a common therapeutic target. Interestingly, we have observed a positive correlation between STAT3 phosphorylation and RhoU expression levels in all tested tumour-derived cell lines. Indeed, phosphorylated STAT3 was undetectable in T47D cells, the only ones displaying very low RHOU mRNA levels. The metastatic prostate cancer cell line PC3 in contrast, also lacking detectable STAT3 activation, displayed high RHOU mRNA expression. In these cells Wnt-1 expression was reported to be extremely elevated, and could thus play a dominant role in RhoU induction bypassing the need for STAT3 activity . A direct correlation between STAT3 activity and RhoU transcription in tumour cell lines is also supported by our observation that specific STAT3 inhibition triggered a dramatic reduction of RHOU mRNA in DU145 and SKBR3 cells. Accordingly, acute STAT3 activation by IL-6 treatment could efficiently up-regulate RHOU mRNA in the non-transformed mammary epithelial cell line MCF10A. Taken together, these results strengthen the hypothesis that RhoU may represent a common transcriptional and functional target of both the Wnt-1 and STAT3 pathways in physiological, as well as pathological, contexts. Further studies addressing the role played by RhoU in mediating specific features of STAT3 and Wnt-mediated cell transformation will be required to address this issue.
SUPPLEMENTARY ONLINE DATA
The RhoU/Wrch1 Rho GTPase gene is a common transcriptional target of both the gp130/STAT3 and Wnt-1 pathways
Davide SCHIAVONE*, Sarah DEWILDE*1, Francesco VALLANIA*2, James TURKSON†, Ferdinando DI CUNTO* and Valeria POLI*3
*Molecular Biotechnology Center and Department of Genetics, Biology and Biochemistry, University of Turin, Via Nizza 52, 10126 Turin, Italy, and
†BioMolecular Science Center and Department of Molecular Biology and Microbiology, University of Central Florida, Orlando, FL 32826, U.S.A.
Figure S1 RhoU induction by OSM and Wnt-1 in HeLa cells
(a) RhoU mRNA was measured by qRT-PCR in HeLa cells treated with OSM for different lengths of time. (b and c) Transient transfection assays. The RhoU −770 and Socs3 (b)or TOPFlash/FOPFlash promoter constructs (c) were co-transfected with−a pSEAP vector in HeLa cells. At 24 h later, cells were either stimulated with OSM (b) or co-cultured with wild-type (NT) or Wnt-1-expressing (Wnt1) HEK-293 cells (c) for 24 h. Results shown are means ± S.E.M. of normalized luciferase activity from at least two independent experiments performed in duplicate.
Received 13 January 2009/1 April 2009; accepted 28 April 2009 Published as BJ Immediate Publication 28 April 2009, doi:10.1042/BJ20090061
1 Present address: BioIndustry Park del Canavese, 10010 Colleretto Giacosa, Italy.
2 Present address: Center for Genome Sciences, Department of Genetics, Washington University in St Louis School of Medicine, 4444 Forest Parkway, St Louis, MO 63108, U.S.A.
3 To whom correspondence should be addressed (email@example.com).
© The Authors Journal compilation © 2009 Biochemical Society
We wish to thank Dr I. Barbieri for help with the MCF10 cells, Dr C. J. Auernhammer for kindly providing the SOCS3 mouse promoter construct, Professor M. Orlandini for the mutant β-catenin (S33A) and the Wnt-1 constructs, Professor S. Rose-John and Professor C. Toniatti for the gift of recombinant plasmids and Professor G. Merlo, Professor E. Calautti and Professor D. Taverna for critically reading the manuscript prior to submission.
This work was supported by the Italian Ministry of University and Research (MIUR COFIN and FIRB) and by the Italian Cancer Research Association (AIRC).