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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Biochem J. Author manuscript; available in PMC 2010 September 1.
Published in final edited form as:
PMCID: PMC2906378

The 5′ terminal uracil of let-7a is critical for the recruitment of mRNA to Argonaute2


Small RNAs modulate gene expression by forming a ribonucleoprotein complex with Argonaute proteins and directing them to specific complementary sites in target nucleic acids. However, the interactions required for the recruitment of the target nucleic acid to the ribonucleoprotein complex are poorly understood. In the present manuscript we have investigated this question by using let-7a, Argonaute2 and a fully complementary mRNA target. Importantly, we have found that recombinant Argonaute2 is sufficient to direct let-7a guided cleavage of mRNA. Thus this model system has allowed us to investigate the mechanistic basis of silencing in vitro and in vivo. Current models suggest that Argonaute proteins bind to both the 5′ and 3′ termini of the guide RNA. We have found that the termini of the let-7a microRNA are indeed critical, since circular let-7a does not support mRNA cleavage. However, the 5′ end is the key determinant, since its deletion abrogates activity. Surprisingly, we have found that alteration of the 5′ terminal uracil compromises mRNA cleavage. Importantly, we have found that substitution of this base has little effect upon the formation of the binary let-7a–Argonaute2 complex, but inhibits the formation of the ternary let-7a–Argonaute2–mRNA complex. Thus we conclude that the interaction of the 5′ uracil base with Argonaute2 plays a critical and novel role in the recruitment of mRNA.

Keywords: Argonaute, microRNA, let-7, mRNA cleavage


It is now well appreciated that small RNAs can modulate gene expression through the formation of a ribonucleoprotein complex that interacts with complementary elements in nucleic acid targets [18]. The interaction of these small RNAs to their target nucleic acids results in a plethora of silencing events, including DNA methylation, mRNA cleavage, mRNA deadenylation and repression of translation [915]. The primary protein component of these ribonucleoprotein complexes is typically a member of the Argonaute family [1623]. This family of proteins was first discovered in the identification of Arabidopsis mutants that developed an aberrant leaf structure that resembles squid tentacles [24,25]. Subsequently, other mutant Argonaute alleles were found in a screen to identify genes involved in plant post-transcriptional gene silencing [26]. A direct role in RNA-directed silencing was later provided by the observation that an Argonaute homologue was necessary and sufficient for the siRNA (small interfering RNA)-mediated cleavage of mRNA [20,27].

At present, Argonaute proteins are understood to contain three functional domains, theMIDdomain that binds to the 5′ phosphate of the small RNA, the PIWI domain that in some cases catalyses cleavage of the mRNA, and the PAZ domain, which is thought to bind to the 3′ end of the guide RNA [2735]. However, most of our current understanding arises from systems that employ a model siRNA of somewhat arbitrary sequence. Importantly, cellular small RNAs are extraordinarily conserved in sequence from worm to man [3638]. Moreover, there is a large family of closely related, but functionally distinct, Argonaute proteins in most organisms [3947]. Therefore we anticipated that there may be sequence-specific interactions between small RNAs and their Argonaute cofactors. Thus, we elected to study the human let7amicroRNA, its Argonaute effector and a fully complimentary target mRNA. We anticipated that this model system would allow us to uncover any sequence-specific interactions in vitro and in vivo. We have found that the 5′ terminal nucleotide of let-7a is involved in a sequence-specific interaction with Argonuate2 which is critical for silencing activity.


Synthetic RNAs were obtained from Dharmacon Research or the University of Calgary UCDNA services (Calgary, Alberta, Canada). All wild-type and mutant microRNAs were synthesized with a 5′ phosphate terminus. Synthetic siRNAs and antagomirs were obtained from Dharmacon Research. The GST (glutathione transferase)–Argonaute2 and GST–Argonaute2 active site mutants were a gift from Professor Leemor Joshua-Tor (HHMI/W.M. Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, U.S.A.). Anti-Argonaute2 antibody was obtained from Upstate Biochemicals and monoclonal antibodies against GAPD (glyceraldehyde-3-phosphate dehydrogenase) and vimentin were obtained from Abcam.

Luciferase reporter assay of let-7a activity

Target elements were subcloned into the SacI and BsteII sites of the pSENSOR dual luciferase reporter plasmid by the ligation of the appropriate DNA duplexes. pSENSOR is a derivative of psiCHECK-2 (Promega) in which 5′-TCGAGGAGCTCTATACGCGTCTCAAGCTTACTGGTTACCGTTCTAGAGTCGGGCCCGGGAATTCGTTTCAGCCTAGGC-3′ was inserted into the Xho1/Not1 sites within the multiple cloning site of psiCHECK-2, creating the SacI and BsteII sites used for cloning. The reporter plasmids, siRNA duplexes, antagomirs and microRNA duplexes were transfected into HeLa human cervical carcinoma cells using Lipofectamine™ 2000 (Invitrogen), according to the manufacturer’s instructions. After 36 h, dual luciferase activities were determined by assaying the cell lysates according to the manufacturer’s protocol (Promega). Renilla luciferase activity was determined by quantitative titration and normalized for transfection efficiency to firefly luciferase activity.

Purification of GST–Argonaute2 protein

An overnight culture of Escherichia coli XL1Blue, transformed with full-length human Argonaute2 cDNA tagged with GST, was diluted 1:50 in LB (Luria–Bertani) medium and grown at 37°C. At a D600 of 0.4, the culture was induced with isopropyl β-d-thiogalactoside (1 mM). After 16 h of further growth at 25°C, cells were spun down and resuspended in 5 ml of Buffer A (50 mM Tris, pH 8.0, and 1 mM EDTA). The cells were lysed by adding lysozyme and Triton X-100 to a final concentration of 0.2 mg/ml and 1% respectively [48]. The lysate was centrifuged at 12000 g for 30 min. The resultant supernatant was incubated with glutathione–agarose (20 mg of protein/ml of resin) for 2.5 h at 4°C prior to addition to the column. After washing the column with Buffer B (50 mM Tris, pH 8.0, 200 mM NaCl, 1 mM EDTA and 0.1% Triton X-100), GST–Argonaute2 was eluted with 50 mM Tris, pH 8.0, and 5 mM glutathione. Active protein was determined by let-7-directed mRNA cleavage activity, pooled and stored at −80°C.

Preparation of labelled RNA

RNAs were labelled using T4 polynucleotide kinase and [γ - 32P]ATP (Amersham Bioscience) to a typical specific activity of 106 c.p.m./pmol. After phenol/chloroform extraction, the labelled RNA was gel purified followed by chloroform extraction and ethanol precipitation [49].

mRNA cleavage

Reaction mixtures (20 µl) contained 50 mMTris, pH 7.5, 50 mM KCl, 1 mM MgCl2, let-7a microRNA and protein as indicated. Mixtures were preincubated at 37°C for 30 min. Following preincubation, 32P-end-labelled target RNA (106 c.p.m./pmol) was added to a final concentration of 1 nM. Mixtures were then incubated at 37°C for 15 min. Following incubation, 80 µl of a dye mixture (98% formamide, 10 mM EDTA, 1 mg/ml Bromophenol Blue, 1 mg/ml Xylene Cyanole) was added. Samples were incubated at 60°C for 2 min and 4%of the reaction mixture was analysed on a 12%(20:1) denaturing polyacylamide gel in TBE buffer. The gel was fixed in 10%acetic acid, dried on DE81 chromatography paper (Whatman) with a backing of gel drying paper and exposed to BioMax MS film.

Identification of the let-7a–Argonaute2 and let-7a–Argonaute2–mRNA complexes by native gel electrophoresis

Reaction mixtures (20 µl) contained 50 mMTris, pH 7.5, 50 mM KCl, 1 mMMgCl2, 0.005%Nonidet P40, 0.2 µg of tRNA, GST–Argonaute2 D597A (active site mutant deficient for cleavage activity) as indicated and 0.1 nM 5′ 32P-end-labelled let-7a RNA (106 c.p.m./pmol). Mixtures were preincubated at 37°C for 30 min. Following preincubation, target RNA was added to the reactions. Mixtures were then incubated at 37°C for 15 min. Then, 4 µl of native loading buffer (50% glycerol, 0.1 M Tris, pH 8.0, 0.1%Bromophenol Blue and 0.1%Xylene Cyanole) was added. Next, 50% of the reaction was analysed by 1% agarose gel in TAE buffer. The gel was dried on DE81 chromatography paper (Whatman) with a backing of gel drying paper and exposed to BioMax MS film.


Human Argonaute2 can utilize let-7a to silence gene expression

We first designed a model element to measure the suppressive activity of Argonaute in human cells. The residues of the 42 nucleotide element from Lin-41 mRNA [50] were made fully complementary to let-7a (Figure 1A). To test whether this element can silence gene expression in human cells, we subcloned it into the 3′-UTR (3′-untranslated region) of Renilla luciferase mRNA using a dual luciferase reporter (pSENSOR) in which transfection efficiency can be normalized by the simultaneous measurement of firefly luciferase. This resulted in a significant (15-fold) decrease of Renilla luciferase expression compared to the parental Renilla luciferase mRNA (Figure 1B). Such silencing might have been exerted by protein factors, so we generated a mutant element in which the putative interaction with the seed sequence of let-7a would be disrupted. The insertion of this seed mutant element into Renilla luciferase mRNA provoked remarkably little silencing (Figure 1B) and was comparable with the parental vector. In the subsequent experiments, we have measured let-7a activity as the fold repression between the seed mutant and wild-type sensor reporters.

Figure 1
Let-7a robustly silences mRNA containing a fully complementary target site derived from lin-41 mRNA

Evidence that the silencing was mediated by let-7a microRNA was provided by the observation that the addition of exogenous let-7a microRNA further stimulated endogenous let-7a silencing activity (Figure 1C). Similarly, the addition of antagomirs against let-7a relieved the silencing effect, whilst an antagomir against an irrelevant microRNA (miR-96) had no effect (Figure 1D). Thus we concluded that this model element significantly silenced expression, and that its effects can be attributed to let-7a. Next, we down-regulated Argonaute2 and ascertained its effect upon the silencing activity of let-7a. siRNA-mediated down-regulation of Argonaute2 significantly attenuated let-7a silencing activity (Figure 2A). On the other hand, the down-regulation of GAPD, as shown by Western blot analysis (Figure 2B), had little effect upon let-7a activity. Thus, we concluded that let-7a can use Argonaute2 to silence gene expression in HeLa cells.

Figure 2
Argonaute2 is necessary for let-7a activity

Recombinant Argonaute2 is sufficient to support the let-7a directed cleavage of mRNA

Given that let-7a can use Argonaute2 to silence gene expression, the next key question was whether purified recombinant human Argonaute2 was sufficient to recapitulate silencing activity in vitro. MicroRNAs are thought to silence gene expression by either translational repression, deadenylation of mRNA or mRNA cleavage [3,10,11,14,15,51]. Currently, the accurate cleavage of mRNA is the most robust and unambiguous in vitro measure of microRNA/Argonaute ribonucleoprotein activity. Thus, we have used mRNA cleavage as the principal assay for the formation of an active let-7a–Argonaute2 ribonucleoprotein complex. Accordingly, we affinity purified a human GST–Argonaute2 fusion protein from E. coli [27]. This preparation was preincubated with let-7a microRNA and then incubated with an end radiolabelled mRNA corresponding to the target element (Figure 3A). Impressively, even after a short incubation (15 min), the mRNA was efficiently cleaved at a position consistent with the scissile phosphate opposite the tenth and eleventh nucleotides from the 5′ end of the let-7a microRNA(Figure 3A). Preincubation of an irrelevant protein (GST) with let-7a did not result in mRNA cleavage. Importantly, cleavage of a smaller target RNA (21 nucleotides in length) resulted in the formation of a smaller cleavage product also corresponding to a position between the nucleotides complementary to the tenth and eleventh nucleotides from the 5′ end of the let-7a microRNA (Figure 3B). The current belief is that the guide RNA–Argonaute complex forms first and then recruits the target mRNA. To test this directly, we performed an order of addition experiment. Figure 3(C) shows that the incubation of Argonaute2 with let-7a followed by the addition of target mRNA leads to a much greater reaction than in the scenario where the mRNA is added first, followed by the addition of let-7a microRNA. Thus, we conclude that the formation of a let-7a–Argonaute2 complex is indeed the obligate first step in the silencing reaction.

Figure 3
Recombinant Argonaute2 is sufficient to support the let-7a-directed cleavage of mRNA

Cleavage required a divalent cation, since no activity was evident in the absence of magnesium or in the presence of EDTA (Figure 4A). Studies on the cleavage reaction directed by the guide strand of an siRNA have indicated that it is probably catalysed by the Argonaute2 DDH (aspartate-aspartate-histidine) catalytic triad in the PIWI domain [27]. Thus we examined whether these residues were also critical for let-7a microRNA-directed cleavage of mRNA. Indeed, we observed that the alteration of any one of these residues to alanine completely abrogated cleavage activity (Figure 4B). Previous studies have drawn attention to the structural similarities between Argonaute2 and ribonuclease H, a DNA-directed RNA endonuclease [27,30]. Similarly, many of the existing structural models for the Argonaute protein employ proteins from archea bacteria that are DNA-directed endonucleases [3032,35]. To test whether recombinant Argonaute2 is an RNA-directed endonuclease, we provided Argonaute2 with DNA corresponding to the let-7a sequence (Figure 4C). We observed that DNA is unable to support cleavage of the target mRNA. Thus, we concluded that Argonaute2 is indeed an RNA-dependent endonuclease. It is important to note that in this experiment and indeed in most of our assays, there is a very minor band that migrates close to, but is distinguishable, from the cleaved mRNA. This minor band probably arises from a contaminant activity, as it is present on incubation with mutant let-7a or catalytically inactive Argonaute2.

Figure 4
Argonaute2 is a RNA-dependent endonuclease that requires magnesium and the integrity of the DDH catalytic domain

The 5′ end of let-7a is critical but the 3′ end is dispensable

The current models of the interaction between the guide strand and Argonaute proteins suggest binding pockets for both the 5′ and 3′ termini [2729,3133]. To test the requirement for the ends of the microRNA in the formation of an active ribonucleoprotein complex, we generated circular let-7a microRNA using RNA ligase [52]. Linear let-7a was treated with RNA ligase and the resultant circles were gel purified. Linear let-7a, not treated with RNA ligase, was carried through the same regimen as a comparison control. Importantly, circular let-7a RNA did not direct mRNA cleavage (Figure 5C), even though both linear and circular let-7a were fully capable of annealing to the target mRNA (Figure 5B). Thus we conclude that indeed the termini of the let-7a microRNA are critical for silencing activity.

Figure 5
Circular let-7a does not support Argonaute2-catalysed cleavage of mRNA

To elucidate whether the 5′ and 3′ ends of let-7a are both important, we created let-7a microRNAs harbouring a four base mismatch at either the 5′ or 3′ end. Since the seed sequence of a microRNA is important for target recognition, it was not surprising that a four base mismatch in the seed sequence of let-7a abrogated its ability to direct mRNA cleavage (Figure 6A). Importantly, a similar alteration at the 3′ end had no visible effect (Figure 6A). Using our reporter assay system, we introduced the corresponding alterations into the target element and measured its effect on let-7a activity in vivo. Similar to the results in vitro, we find that complementarity at the 5′ end of let-7a is critical for silencing activity, whereas mutation of the 3′ had no discrete effect (Figure 6B). These observations suggest that the let-7a–Argonaute2 ribonucleoprotein complex can silence a partially complementary mRNA target in vitro and in vivo.

Figure 6
The 5′ end of let-7a is critical but the 3′ end is dispensable for Argonaute2-catalysed cleavage

To further study the interactions of the 5′ and 3′ end of let-7a, we generated let-7a mutants containing deletions at either the 5′ or 3′ end. A five nucleotide deletion at the 3′ end had little effect whereas a more extensive deletion significantly attenuated silencing activity (Figure 6C). Thus this extends our previous observation and asserts that the let-7a–Argonaute complex can silence a partially complementary target mRNA. Strikingly, deletion of the 5′ end of let-7a abrogated its ability to direct mRNA cleavage. Previous studies have shown that deletion of the 5′ end of a guide siRNA did not preclude mRNA cleavage, but resulted in the formation of a new cleavage site [27]. This has been attributed to the ability of the de novo terminus of the siRNA to ‘slide’ into the MID domain phosphate-binding pocket and thereby direct a new cleavage site. Since deletion of the 5′ end of let-7a microRNA abrogated cleavage and no new cleavage site was created, we speculate that let-7a is unable to ‘slide’ in the binding groove of Argonaute2.

The 5′ uracil residue of let-7a is critical to direct mRNA cleavage in vitro and in vivo

Finally, we examined each residue of the let-7a microRNA. Sequential mutation of residues 2–20 had little effect (Figure 7A) on mRNA cleavage. Only the residues which surround the cleavage site (9–12), had any significant effect upon silencing activity. However, to our surprise, the 5′ terminal nucleotide (residue 1) was critical. We substituted the 5′ terminal uracil of let-7a with adenine, guanine or cytosine and found that Argonaute2-directed cleavage required a uracil terminus (Figure 7B). Although a very small amount of activity was seen with an adenine terminus, no activity was apparent with let-7a microRNA containing cytosine or guanine at the terminus. Thus, we concluded that we had probably disrupted a critical interaction between the terminal base of let-7a and amino acids surrounding the phosphate-binding pocket. The 5′ terminal nucleotide is not thought to interact with the target mRNA [34,53]; however, our target mRNA contains an adenine residue that could potentially base pair to the terminal uracil. It was possible that the disruption of this interaction was responsible for the loss of mRNA cleavage activity. To test this, we utilized a shorter mRNA target that lacks this residue. This truncated mRNA was also robustly cleaved by the let-7a/Argonaute2 complex and displayed the same requirement for uracil at the 5′ terminus of let-7a (Figure 7C). Thus we conclude that the uracil base is important for an interaction with Argonaute2, rather than an interaction with mRNA.

Figure 7
The 5′ uracil residue of let-7a is critical to direct mRNA cleavage in vitro and in vivo

To confirm these observations at the cellular level, we generated let-7a microRNA duplexes in which the 5′ terminus of the microRNA had been similarly altered. These microRNA duplexes were transfected into HeLa cells and their silencing activity was measured by their ability to stimulate endogenous let-7a activity as measured by the reporter assay. As observed in vitro, only the let-7a duplexes with a uracil at the 5′ end of the microRNA were capable of efficiently silencing expression in HeLa cells (Figure 7D). It is important to note these cellular experiments could not be conducted with single-stranded microRNA. Thus, there is a possibility that the 5′ terminal alterations may affect the loading of let-7a into Argonaute. However, in the case of the uracil to cytosine alteration the 5′ end of the guide strand remains in an open configuration. Thus, we attribute the effects of the mutants to the reduced cleavage activity of the let-7a–Argonaute2 complex.

The 5′ uracil residue of let-7a is critical for the recruitment of mRNA to the Argonaute2 silencing complex

Next we investigated whether the 5′ terminal uracil was critical for the formation of the let7a–Argonaute complex itself or for the subsequent step of the recruitment of mRNA. Although small RNA–Argonaute complexes have previously been identified by crosslinking analysis [27,54], it has not yet been possible to resolve the postulated binary microRNA–Argonaute complex from the ternary microRNA–Argonaute–mRNA complex. Thus, we investigated whether the binary and ternary complexes could be resolved by native gel electrophoresis. To more readily visualize these reaction intermediates, we used the Argonaute2 catalytic mutant (D597A), which we anticipated may trap the let-7a–Argonaute2–mRNA complex. Incubation of radiolabelled let-7a with recombinant human Argonaute2 led to the appearance of a slow migrating species indicative of the let-7a–Argonaute2 complex (Figure 8A). Such a complex was not apparent on incubation with an irrelevant protein (GST). Importantly, this putative let7a–Argonaute2 complex could be shifted to a slower migrating species with the addition of the target mRNA in a concentration-dependent fashion (Figure 8B). This complex was not identified on incubation with GST or upon addition of an irrelevant target mRNA. To investigate whether the mRNA was annealed to let-7a in the complex, we treated the reaction with SDS and looked to see whether the let-7a–mRNA duplex was released. As predicted, SDS dissolved complex formation (Figure 8B) and indeed led to an increased amount of a let-7a–mRNA duplex on analysis by polyacrylamide gel electrophoresis (Figure 8C). The relatively small amount of let-7a–mRNA duplex observed in the absence of SDS may arise from the turnover of Argonaute2 complexes or the direct annealing of let-7a to mRNA. Thus we conclude that this approach can resolve the let-7a–Argonaute2 and let-7a–Argonaute2–mRNA complexes and may be utilized to study the interactions required for the recruitment of mRNA to the Argonaute silencing complex.

Figure 8
The 5′ uracil residue of let-7a is critical for the recruitment of mRNA to the Argonaute2 silencing complex

Next we sought to establish whether the 5′ terminal uracil was important for the formation of the let-7a–Argonaute2 complex itself, or for a subsequent step in the silencing reaction. Alteration of the uracil base did not affect the formation of the let-7a–Argonaute2 complex (Figure 8D). However, only the microRNA that contained uracil at the terminus was capable of efficiently forming the let-7a–Argonaute2–mRNA complex. This observation suggested that an appropriate occupancy of the 5′ binding pocket by let-7a is necessary to recruit mRNA and direct mRNA cleavage.


It is well established that small-RNA-directed silencing plays a critical role in the regulation of gene expression [36]. However, the fundamental steps in this pathway, the formation of the microRNA ribonucleoprotein complex and its recruitment of mRNA, have remained poorly understood. In the present study, we have chosen to study the mechanism of action of let-7a and Argonaute2 using a fully complementary model target mRNA. Importantly, we show that recombinant human Argonaute2 is sufficient to direct mRNA silencing. Similar to studies with siRNA [20,27,55], let-7a-directed cleavage of mRNA requires the divalent cation, magnesium, and the integrity of the Argonaute2 DDH catalytic domain.

Little attention has been paid to the possibility that human microRNAs may silence via mRNA cleavage. Largely, this is because it has been thought that complete complementarity is required, and contemporary sequence analysis indicates that there are very few human mRNAs that contain elements that are fully complementary to microRNAs [5661]. However, we show here that complete complementarity is not critical and therefore it is quite likely that let-7amay silence some cellular mRNAs. It is also thought that the ability to cleave mRNA is unique to Argonaute2. This has also contributed to the notion that microRNA-directed silencing via mRNA cleavage would be a rare event. However, the experiments to test the cleavage activity of Argonautes 1, 3 and 4 were conducted with a guide RNA that contained a uracil at its 5′ terminus [23,62]. Thus, it is quite possible that these Argonautes have a different specificity and it might be interesting to re-examine their catalytic potential by providing them with guide RNAs that have other nucleotides at their 5′ terminus.

Previous studies have also illuminated the critical role of the 5′ ends of microRNA and the guide strand of siRNA [3,59,61,6366]. However, in those studies, the deletion of the 5′ end of the guide strand of siRNA did not preclude mRNA cleavage, but resulted in a new cleavage site [27]. The most reasonable interpretation of those studies is that the new 5′ terminus of the siRNA can enter the phosphate-binding pocket and thus ‘move’ the cleavage site accordingly. This is also consistent with the observation that the 3′ end of the guide RNA can be deleted without compromising cleavage activity. However, in our experiments, deletion of the 5′ end of let-7a microRNA abrogated cleavage, and no new cleavage site was created. Importantly, the deletion recreated a uracil base at the 5′ end. Despite this, it would appear that the new terminus cannot occupy the 5′ phosphate-binding site and redirect cleavage. This suggests that there are likely to be specific interactions between the other residues of let-7a and Argonaute2, and that these are stronger than those involved in the 5′ pocket binding. Thus the development of dynamic analytical techniques that can distinguish interactions at the 5′ end from those at the body of the let-7a microRNA is a critical future endeavor. In summary, our observations suggest that sequence-specific interactions between the let-7a microRNA and Argonaute2 might be more important than have been suspected.

The major observation here is that the 5′ terminus of the guide RNA plays a unique sequence-specific role in the recruitment of mRNA. Structural studies on Archaeoglobus fulgidus and Thermus thermophilus PIWI complexes have shown that the 5′ phosphate of the guide molecule is complexed to amino acid residues and Mg2+ [27,32,35]. Interestingly, these studies show that the terminal base (in this case thymine) can interact with the side chain of an arginine residue. It is likely that substitution of the terminal base will weaken this interaction and perhaps preclude the engagement of the 5′ end with the binding pocket. Alternatively, this substitution may result in an inappropriate engagement that alters the accessibility of the guide RNA to mRNA. At present, we cannot distinguish these possibilities. It is important to point out that there are many functional microRNAs and siRNAs that do not have a uracil residue at the 5′ terminus. From our studies in the present manuscript, it would appear unlikely that these RNAs efficiently utilize the Argonaute2 family member. Indeed, one suspects that, as is the case in plant cells, each Argonaute family member will associate with particular classes of small RNAs directed by the nature of the 5′ terminus [67,68].

Finally, our observations suggest an additional consideration to the models that seek to explain the selective loading of the let-7a guide strand of the precursor microRNA duplex into Argonaute2 [69]. Given that the two strands of microRNA precursor duplexes usually have different bases at the 5′ termini, it is now plausible that Argonaute2 itself exerts some specificity in strand uptake. Indeed, we have recently shown that let-7*, which contains a cytosine residue at its 5′ terminus, is remarkably inefficient in supporting mRNA cleavage (D. W. Salzman, K. M. Felice and H. M. Furneaux, unpublished work).



This work was supported by the National Institutes of Health [grant numbers R03 DA022226, P01HL70694].

Abbreviations used

DDH catalytic triad
aspartate-aspartate-histidine catalytic triad
glyceraldehyde-3-phosphate dehydrogenase
glutathione transferase
small interfering RNA
3′-untranslated region



Kristin Felice performed research, designed experiments, analysed data and wrote the manuscript. David Salzman performed research, designed experiments and analysed data. Jonathan Shubert-Coleman performed research. Kevin Jensen performed research. Henry Furneax wrote the manuscript, designed experiments and analysed data.


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