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Oecologia
 
Oecologia. 2010 August; 163(4): 1033–1042.
Published online 2010 March 28. doi:  10.1007/s00442-010-1604-6
PMCID: PMC2903711

Changes in herbivore control in arable fields by detrital subsidies depend on predator species and vary in space

Abstract

Prey from the decomposer subsystem may help sustain predator populations in arable fields. Adding organic residues to agricultural systems may therefore enhance pest control. We investigated whether resource addition (maize mulch) strengthens aboveground trophic cascades in winter wheat fields. Evaluating the flux of the maize-borne carbon into the food web after 9 months via stable isotope analysis allowed differentiating between prey in predator diets originating from the above- and belowground subsystems. Furthermore, we recorded aphid populations in predator-reduced and control plots of no-mulch and mulch addition treatments. All analyzed soil dwelling species incorporated maize-borne carbon. In contrast, only 2 out of 13 aboveground predator species incorporated maize carbon, suggesting that these 2 predators forage on prey from the above- and belowground systems. Supporting this conclusion, densities of these two predator species were increased in the mulch addition fields. Nitrogen isotope signatures suggested that these generalist predators in part fed on Collembola thereby benefiting indirectly from detrital resources. Increased density of these two predator species was associated by increased aphid control but the identity of predators responsible for aphid control varied in space. One of the three wheat fields studied even lacked aphid control despite of mulch-mediated increased density of generalist predators. The results suggest that detrital subsidies quickly enter belowground food webs but only a few aboveground predator species include prey out of the decomposer system into their diet. Variation in the identity of predator species benefiting from detrital resources between sites suggest that, depending on locality, different predator species are subsidised by prey out of the decomposer system and that these predators contribute to aphid control. Therefore, by engineering the decomposer subsystem via detrital subsidies, biological control by generalist predators may be strengthened.

Keywords: Food web, Energy subsidy, Pest control, Aphids, Stable isotopes

Introduction

Generalist predators, including carabids, staphylinids and spiders, are among the most important predators in terrestrial arthropod systems. They effectively decrease prey populations including agricultural pest species (Wise 1993; Lövei and Sunderland 1996; Symondson et al. 2002). Due to their catholic feeding, they may lack attributes of the ideal biological control agent such as prey specialisation. However, including non-pest prey in their diet may sustain generalist predator populations in fields when pest prey is scarce (Symondson et al. 2002), an important feature lacking in specialists. Periodic feeding by generalist predators on alternative prey from the detrital subsystem may strengthen herbivore control through apparent competition (Polis 1994; Polis and Strong 1996; Scheu 2001; Bell et al. 2008). Decomposer organisms generally appear to be limited by the availability of dead organic matter (Hairston et al. 1960; Hairston 1989; Scheu and Schaefer 1998), and therefore the detrital food web may be manipulated through application of allochthonous resources.

There is evidence that fostering the decomposer subsystem through detrital subsidies may increase densities of generalist predators in rice (Settle et al.1996) and vegetable garden systems (Halaj and Wise 2002). However, increased densities of generalist predators may also enhance intraguild predation and cannibalism. Both are common in terrestrial food webs (Polis et al. 1989; 1991) and have been suggested to dampen biological control (Snyder and Wise 2001; Lang 2003; Wise 2006). Further, generalist predators may not switch to pest species if alternative prey is in ample supply (Birkhofer et al. 2008). Especially aphids are of low food quality or even toxic for generalist predators (Toft 2005; Oelbermann and Scheu 2002), possibly preventing generalist predators from including these pest species in their diet. For a better understanding of the factors affecting pest suppression, information on trophic links and food web structure above- as well as belowground of arable systems is needed.

The analysis of natural variations in stable isotope ratios is a promising tool to investigate trophic interrelationships, especially in food webs where generalist predators dominate (Ponsard and Arditi 2000; Scheu and Falca 2000; Scheu 2002). The concentration of 15N in consumers increases at higher trophic levels and this allows determining the trophic position of species in food webs (DeNiro and Epstein 1981; Minagawa and Wada 1984; Post 2002). In contrast to 15N, carbon isotopes are little fractionated in consumers (Peterson and Fry 1987; Post 2002) and allow the determining of food resources of consumers. Differences in carbon isotope ratios of resources have been used to trace carbon fluxes and determine the fractions of different food sources in animal diets (Fry et al. 1978; Martin et al. 1992; Pollierer et al. 2007). C4 and C3 plants markedly differ in their 13C/12C ratio and maize (C4 plant) as a food resource for the decomposer subsystem has been used before to trace carbon fluxes into the soil food web of arable systems (Albers et al. 2006). They found the maize carbon to be rapidly incorporated into the decomposer system; however, further carbon fluxes into the aboveground subsystem were not evaluated.

In the present study, we investigated the effect of detrital subsidies (maize mulch) on the decomposer subsystem and its feedbacks on the aboveground generalist predator–herbivore system in winter wheat. To consider variations in predator–prey relationships in space three wheat fields embedded in different landscapes were investigated. We hypothesised that (1) an allochthonous resource (maize mulch) boosts decomposer densities with effects propagating into the next growing season; (2) generalist predators feed on prey from the decomposer subsystem, resulting in increased predator densities in mulched fields and as a consequence, (3) to increased suppression of herbivore populations.

Materials and methods

Study site

The experiment was conducted between September 2003 and August 2004 in three winter wheat fields managed by the Reinshof research farm of the University of Göttingen (Lower Saxony, Germany). Two of the three investigated fields were located near the River Leine at 150 m above sea level. Field 1 was surrounded by cereal and root crop fields; field 2 was close to the city of Göttingen, adjacent to a garden colony. At these sites, loamy flood-plain soils established on clayey silt predominate. Field 3 was located in the north of Göttingen at 320 m above sea level; the soil at this site was shallow (Rendzina) and formed on shell limestone. It was surrounded by hedgerows and groves, embedded in a diverse landscape of a mixture of forests, hedgerows, and pastures. The mean annual temperature in Göttingen is 8.7°C, and the mean annual precipitation is 645 mm. Mean temperature was higher than average during both years of the study, mean rainfall was lower in 2003 and higher in 2004 than average (9.4°C and 550 mm in 2003, 9.1°C and 718 mm in 2004).

Experimental setup and sampling

In each field, two randomly chosen areas of 1 ha each received 15 t (fresh weight) of maize chaff in September 2003. The maize chaff was equally distributed within the 1 ha areas and homogenised with the upper soil layer by grubbing. Subsequently, all three fields including the mulched areas were sown with winter wheat. In May 2004, experimental treatments were established in a 2 × 2 × 2 factorial design with the factors ‘mulch’ (with and without), ‘soil dwelling predators’ (SP, reduced and open control) and ‘flying predators’ (FP, reduced and open control) with two replicates per field. To reduce soil dwelling predators, plastic barriers were installed reaching 10 cm into the soil and 40 cm above the ground enclosing a circular area of 2 m2. Four ‘live’ pitfall traps (without trapping liquid) situated at the inner edge of the barriers were established in a cross design. Pitfalls were cleared daily over a period of 19 days throughout June. All predators, i.e. carabid and staphylinid beetles, and lycosid spiders, were visually identified, counted and released outside the plots. All other animals were returned to the plots. To reduce flying aphid predators and parasitoids, wire cages (mesh size 8 mm) were set over the plots at the end of June. Cages were sprayed with non-toxic glue (Soveurode Aerosol, Witasek, Austria) to capture flying arthropods.

In each plot, one pot (diameter 25 cm, height 25 cm) planted with wheat of the same variety as in the fields was buried with the edging of the pot at ground level enabling access by surface-active arthropods. The pots were established to test for indirect mulch effects on aphid population development via changing predator control; direct effects due to mulch-mediated changes in nutrient availability were excluded by filling the pots uniformly with soil from the respective non-mulch fields. Aphids were counted visually on 25 wheat shoots inside and outside the pot at milk ripening in mid-July.

To determine the densities of soil dwelling arthropods, including generalist predators and surface active Collembola, plastic barriers as described above were installed in each of the three fields in both the mulch and no-mulch treatments and replicated four times. Four pitfall traps containing an oversaturated saltwater solution were established in a cross design at the inner edge of the barriers. Placing pitfall traps inside the fenced plots allowed taking the catches as density measures as the specimens were caught from a fixed area. However, the catches also reflect the activity of the species caught and more active species therefore may be over-represented. The pitfall traps operated for 2 weeks on three dates, respectively (May 26–June 9, June 23–July 7, July 21–August 4). When pitfall traps were not operating they were closed and the plastic barriers were lifted 15 cm above ground to enable recolonisation by arthropods. Additionally, one soil core (diameter 21 cm) adjacent to each of the plots was taken at the same dates pitfall traps were opened. Soil animals were extracted from the upper 10 cm of the soil cores by heat (Kempson et al. 1963). Invertebrates from the pitfall traps as well as from the soil cores were determined to genus or species level, counted, and stored in oversaturated saltwater solution at −10°C until they were processed for stable isotope analysis.

13C and 15N analysis

Wheat plants from the experimental plots as well as maize chaff were dried at 60°C for 2 days, ground and dried again at 60°C for 1 day. Samples of ~2.6 mg dry mass were prepared for 13C and 15N analysis. Animals were washed in distilled water and dried at 60°C for 6 days. Either homogenised animal tissue or whole animals (80–1,660 μg) were used for stable isotope analysis. In mesofauna species, two or more specimens were bulked per sample to reach appropriate sample weight. Samples were kept in a desiccator until mass spectrometer analysis.

Samples were analysed by a coupled system consisting of an elemental analyser (NA 1500; Carlo Erba, Milan, Italy) and a gas isotope mass spectrometer (MAT 251; Finnigan, Bremen, Germany). The system is computer controlled allowing online measurement of 13C and 15N (Reineking et al. 1993). As primary standards for the isotope values of carbon and nitrogen, Pee Dee Belemnite (PDB) limestone and atmospheric air were used, respectively. Acetanilide (Merck, Darmstadt, Germany) was used for internal calibration. Isotope natural ratios were expressed using the delta notation with δ13C or δ15N (‰) = (Rsample − Rstandard)/(Rstandard × 1,000), where Rsample and Rstandard represent the 13C-to-12C or 15N-to-14N ratio in samples and standard, respectively. Incorporated maize-borne carbon in animals tissue (xm) was calculated by a two-source mixing model with xm (%) = (δam − δaw)/(δpm − δpw) × 100; δam and δaw refer to the δ13C signature of an animal species in the plots with and without maize-mulch, respectively, and δpm and δpw to the δ13C signature of the maize-mulch and the wheat plants, respectively.

Statistical analysis

Data on densities of soil dwelling predator and Collembola species were analyzed by repeated measures (RM) ANOVA with the fixed factors ‘field’ (1, 2, 3) and ‘mulch’ (with, without), and the three consecutive sampling dates as repeated factor. Univariate analyses were performed to inspect interactions if significant in RM-MANOVA and because RM-MANOVA is less powerful than the univariate counterpart for small sample size (Cole and Grizzle 1966; Potvin et al. 1990). Within-subject probabilities were Huynh–Feldt corrected as this adjustment performs well in terms of type I error and power (Stiger et al. 1998) and is recommended by Potvin et al. (1990). In case of significant interactions between one or both of the fixed factors and sampling date, two-factor ANOVA was used to analyse the effects of ‘field’ and/or ‘mulch’ at separate sampling dates. Where appropriate, Bonferroni-corrected post hoc tests were performed to identify differences between treatments.

Data on aphid densities were analysed by analysis of variance (ANOVA) with the dependent variable ‘aphids’ (numbers per shoot) and the independent variables ‘pot’ (yes, no), ‘field’ (1, 2, 3), ‘mulch’ (with, without), ‘soil dwelling predators’ (SP, reduced and control) and ‘flying predators’ (FP, reduced and control). Data were square root transformed prior to the analyses.

Data on δ15N and δ13C signatures were analysed by single factor ANOVAs with the independent variable ‘mulch’ using the GLM procedure to account for unequal cell sizes. Statistical analyses were performed using Statistica 7.1 (StatSoft, Tulsa, USA).

Results

Soil animals

From soil cores, the following species were extracted: Pergamasus sp. Berlese (Mesostigmata); Blaniulus guttulatus F. (1798), Brachyiulus pusillus Leach (1815), Polydesmus inconstans Latzel (1884) (Diplopoda); Lithobius microps Meinert (1868), Necrophloeophagus longicornis Leach (1858) (Chilopoda); Symphyla; Onychiurus spp. Gervais (1841) (Collembola); elaterid larvae (Coleoptera); sciarid larvae and tipulid larvae (Diptera). Densities of all these taxa were too low for statistical analysis, therefore individuals from the different sampling dates were pooled and used for stable isotope analysis.

Predators

With the ‘live’ pitfall traps, 728 staphylinids, 376 carabids and 87 lycosids were removed from the reduced soil-dwelling predator and the reduced flying- and soil-dwelling predator treatments during 19 days in June. On average, ratios between predator numbers removed from mulch and no-mulch plots were 64:36 for rove beetles, 60:40 for carabid beetles and 31:69 for lycosids.

With the saltwater-filled pitfall traps, 1,094 carabids (34 species), 180 carabid larvae, 509 staphylinids (12 genera) and 353 staphylinid larvae (5 subfamilies) were captured. As staphylinids were affected by sampling date, field and mulch (RM-ANOVA; F4,36 = 3.34, P = 0.019 for date × field × mulch) and carabids by sampling date and field (RM-ANOVA; F4,36 = 11.09, P < 0.001 for date × field), RM-ANOVAs were calculated for the most abundant staphylinid and carabid species.

In staphylinids densities of Philonthus spp. (mainly P. fuscipennis) were significantly higher in the mulch treatment in field 1 at all three sampling dates (RM-ANOVA; F2,18 = 7.20, P = 0.005 for field × mulch; Fig. 1a). Densities of Oxytelus inustus were significantly higher in the mulch treatment in fields 2 and 3 at the first sampling date and in the mulch treatment in field 3 at the second sampling date (RM-ANOVA; F2,36 = 5.47, P = 0.008 for date × mulch; Fig. 1b). Densities of staphylinid larvae were significantly increased in the mulch treatment in each of the fields at the second sampling date (RM-ANOVA; F4,36 = 7.36, P < 0.001 for date × field; Fig. 1c).

Fig. 1
Mean population densities [(numbers + 1) ± SE per 2 m2; note log-scale] of soil surface-dwelling generalist predators (af) and epigeic Collembola captured by pitfall traps (gi) in no-mulch ...

In carabids, densities of Trechus quadristriatus were significantly higher in the mulch treatments in all fields at the first and second sampling date (RM-ANOVA; F4,36 = 2.73, P = 0.044 for date × field × mulch; Fig. 1d); at the third sampling date, densities were still significantly higher in the mulch treatment but only in field 2. Densities of Notiophilus biguttatus, Notiophilus palustris, Loricera pilicornis (Carabidae) and Stenus sp. (Staphylinidae) were pooled as ‘Collembola feeders’ (Weinreich 1968; Thiele 1977). These were enhanced significantly in the mulch treatment in field 3 (RM-ANOVA; F2,36 = 3.61, P = 0.037 for date × mulch; Fig 1e). Abundances of carabid larvae were significantly higher in mulch plots in all three fields at the first sampling date (RM-ANOVA; F4,36 = 2.69, P = 0.046 for date × field; Fig. 1f).

Collembola

With pitfall traps, 29,447 Lepidocyrtus cyaneus, 25,011 Isotoma viridis and 2,516 Entomobrya lanuginosa were captured in all three fields during the three consecutive trapping periods. The abundance of L. cyaneus was lower in mulch plots in field 2 at the first, second and third sampling date (RM-ANOVA; F2,36 = 6.32, P = 0.004 for date × mulch); however, only the latter was significant (Fig. 1g). Abundances of I. viridis were significantly higher in mulch plots in field 1 at the first sampling date but significantly lower in mulch plots in field 2 at the second and third sampling dates (RM-ANOVA; F4,36 = 4.89, P = 0.003 for date × field × mulch; Fig. 1h). Abundances of E. lanuginosa were significantly higher in field 1 but lower in field 2 at the first sampling date (RM-ANOVA; F4,36 = 7.86, P < 0.001 for date × field; Fig. 1i).

13C and 15N analysis

The δ15N signatures of the 13 soil-dwelling species captured by heat extraction spanned over two trophic levels in the mulch treatment (Fig. 2). The first trophic level comprised decomposers such as the diplopods P. inconstans, B. pusillus and B. guttulatus, as well as the Collembola Onychiurus spp. and sciarid larvae. δ15N signatures of decomposers formed a continuum from 1.0 to 3.1‰. The carabid species T. quadristriatus was also placed in the decomposer trophic level, with similar δ15N signatures to the diplopod B. guttulatus. The upper trophic level consisted of predators such as the centipedes L. microps and N. longicornis and the gamasid mite Pergamasus sp., the Symphyla and the carabid O. inustus. Also, δ15N signatures of elaterid larvae were similar to those of predators. In contrast, the sampled elaterid larvae in the no-mulch treatment had a δ15N signature of only 2.9 ‰.

Fig. 2
Mean δ15N values and fraction of maize-borne carbon in the body tissue (% of total, black pie) of soil dwelling taxa captured by heat extraction which incorporated maize carbon (means with SD given below taxon name). The lower dashed line represents ...

The incorporated maize-borne carbon ranged between 15% in the Symphyla and 65% in the diplopod B. pusillus (Fig. 2). The range of percentages of incorporated maize-borne carbon in decomposer species (20–65%) was similar to that in predator species (15–56%).

The δ15N signatures of soil surface species captured by pitfall traps spanned over 6.7 and 7.2 ‰ in the mulch and no-mulch treatment, respectively (Fig. 3). Assuming enrichment in 15N of about 3‰ per trophic level (Minagawa and Wada 1984; Post 2002), the 18 species studied in the no-mulch and the mulch treatments spanned over three trophic levels. In the no-mulch treatment, the two Collembola species E. lanuginosa and I. viridis had similar δ15N signatures to the carabids T. quadristriatus and Bembidion obtusum. In the mulch treatment, δ15N signatures of the Collembola E. lanuginosa and I. viridis were 1.1‰ (F1,3 = 4.82, P = 0.11) and 1.4‰ (F1,4 = 9.03, P = 0.040) lower compared to the no-mulch treatment, thereby building a distinct group with L. cyaneus between the two aphid species and the predators. Generally, δ15N signatures of predators were similar in mulch and no-mulch treatments.

Fig. 3
Mean δ15N values and SD of soil surface dwelling species captured by pitfall traps in the no-mulch (a) and mulch (b) treatments

Only 2 of the 18 species of the pitfall catches significantly differed in their δ13C signatures between the mulch and the no-mulch treatments. The body of the carabid T. quadristriatus (F1,4 = 26.08, P = 0.0070) and the staphylinid O. inustus (F1,4 = 33.00, P = 0.0046) contained 50 and 49% of maize-borne carbon, respectively. Interestingly, only these two species were found in both the pitfall traps and the soil cores.

Aphids

The dominant aphid species in all three fields was Sitobion avenae, representing 91.1% of total aphid numbers, followed by Rhopalosiphum padi (6.3%) and Metopolophium dirhodum (2.6%). Aphid infestations averaged 19.8 individuals per shoot, being markedly above the threshold level of economic damage (5 aphids per shoot; Giller et al. 1995). Aphid numbers on wheat plants growing in the pots and wheat plants growing in the field did not differ, nor were there any significant interactions between the factor ‘pot’ and any of the other factors; the factor ‘pot’ therefore was eliminated from the statistical model (Table 1).

Table 1
ANOVA table of F values for the effect of field (1, 2, 3), mulch (yes/no), soil dwelling predators (SP; reduced, control) and flying predators (FP; reduced, control) on numbers of aphids per shoot in mid July

Total aphid numbers differed significantly between the three fields, with 6- and 22-fold higher aphid numbers in field 1 compared to field 2 and field 3 (Table 1, Fig. 4a). However, predator effects differed significantly between fields. In field 1, aphid numbers were only decreased in plots with both soil-dwelling and flying predators (−34%). In field 3, soil-dwelling and flying predators significantly decreased aphid numbers by 80 and 85%, respectively, whereas in field 2 there were no effects on aphid populations in any of the predator treatments. Further, the effect of soil-dwelling and flying predators differed significantly between the mulch and the no-mulch treatments, with the reduction in aphid numbers being restricted to the mulch plots (Table 1, Fig. 4b). In these plots, presence of each soil-dwelling and flying predators decreased aphid numbers with the effect being most pronounced in the combined treatment (−54%).

Fig. 4
Aphid populations (given as aphid numbers per shoot) as affected by flying (FP) and soil dwelling predators (SP) in the three different fields (a) and in the no-mulch and mulch treatments (b); 0 = SP+SF removal, FP = SP ...

Discussion

Soil food web—maize-borne carbon incorporation

About 9 months after the application of the maize chaff, maize-borne carbon could be detected in all taxa of the sampled soil fauna, indicating ubiquitous utilisation of the mulch material. The percentages of incorporated maize carbon in soil fauna species varied between 15 and 65%, and differed between predators and decomposers. In decomposers, the maize-borne carbon contributed on average 43% to animal tissue carbon, whereas predators on average contained 34% maize-borne carbon. This difference in incorporation of maize carbon between trophic groups is similar to that found previously (Albers et al. 2006), and presumably reflects the time lag in carbon incorporation in higher trophic levels.

Assuming a trophic level shift in δ15N signatures of 3‰ (Minagawa and Wada 1984; Post 2002), the soil food web consisted of three trophic levels. Soil food webs in agricultural fields investigated by Moore (1994) varied between 2.3 and 4.2 trophic levels, and Albers et al. (2006) also found three trophic levels in their agricultural soil food web. The δ15N signatures of the species analysed formed a gradient rather than discrete trophic levels. However, the δ15N signatures of the predators spanned only 1.5 δ15N units and did not differ between large and small predators, suggesting that predator species consisted of a single trophic level with little evidence for intraguild predation and cannibalism. In contrast, δ15N signatures of decomposers spanned 4.5 δ15N units, suggesting that decomposers consist of two trophic levels, i.e. primary and secondary decomposers as indicated earlier (Scheu and Falca 2000; Albers et al. 2006).

Combining the δ15N and δ13C signatures may allow identification of trophic links. With similar contents of maize carbon and about 2.5 δ15N units higher than the Collembola Onychiurus spp., Symphyla, L. microps and Pergamasus sp. presumably fed on this species; similarly, Albers et al. (2006) assumed Symphyla and Gamasina to prey on Onychiurus spp. As the δ15N signature of elaterid larvae was similar to that of the predatory mite Pergamasus sp. and the centipede N. longicornis, they presumably live as predators; recent studies suggest that this is widespread in elaterid larvae (Traugott et al. 2007). Interestingly, δ15N signatures of the diplopod B. guttulatus exceeded those of the other two diplopod species, being similar to the δ15N values of the centipede L. microps. As suggested previously, B. guttulatus presumably at least partly lives on an animal diet, e.g. by feeding on carcasses (Hoffman and Payne 1969).

Aboveground food web—maize-borne carbon incorporation

Only two of the sampled species in our study, the carabid T. quadristriatus and the staphylinid O. inustus, were abundant below- and aboveground as indicated by trapped individuals in both the soil cores and the pitfall traps. Interestingly, only these 2 species incorporated maize-borne carbon, whereas in none of the other 16 species studied, including 13 predators and 3 Collembola species, could maize-borne carbon be detected. This suggests that most predators but also major decomposer taxa such as Collembola did not benefit from increased supply of litter resources. C4 plants, such as maize, represent low quality food for herbivores and detritivores due to low nitrogen and high fibre content (Caswell and Reed 1976; Boutton et al. 1978; Ehleringer et al. 2001). Omnivory is probably the prevailing feeding strategy in Collembola (Filser 2002); depending on the resources available they ingest bacteria, fungi, algae, plant litter, or other soil animals, such as protozoa, nematodes, rotifers, and enchytraeids (Parkinson 1988; Scheu 2002; Chahartaghi et al. 2005). Potentially, the three epigeic Collembola predominantly fed on algae; at least, they were not linked trophically to the maize carbon pool.

Consistent with the fact that maize-borne carbon did not enter the majority of species of the aboveground food web, the maize mulch affected their δ15N signatures only a little. However, δ15N signatures of the two Collembola species, I. viridis and E. lanuginosa, decreased in the mulch plots, thereby placing them between the two aphid herbivores and the predators. Presumably, the Collembola species increased feeding on more basal food sources, potentially algae with low δ15N signatures. Some predator taxa showed similar decreases in their δ15N signatures, but not significantly; still, this suggests that these predators mainly feed on Collembola. Further, δ15N signatures of all predator species in both the no-mulch (except for the Tachyporinae larvae) and the mulch treatments more than 3‰ higher than those of the two aphid species is consistent with a mixed diet consisting of Collembola and aphids. Including Collembola and aphids in mixed diets improves development, survival and egg production of generalist predators (Toft 1995; Borg and Toft 1999; Bilde et al. 2000; Oelbermann and Scheu 2002), and complementary predation on aphids and Collembola was previously shown in a field experiment in winter wheat (von Berg et al. 2009).

Effects of mulch on predator densities and feedbacks on aphid suppression

Of the 34 carabid beetle species identified, only 1 species (T. quadristriatus) was significantly increased in density in the mulch treatment. Furthermore, out of the 12 sampled staphylinid genera, the density of only 2 species (P. fuscipennis and O. inustus) were significantly increased in the mulch treatment. Except P. fuscipennis, these species incorporated maize-borne carbon, suggesting that they in fact benefited from the maize resource, presumably through feeding on decomposer prey, most importantly Collembola. Additionally, the densities of carabid and staphylinid larvae, and ‘Collembola feeders’, were increased in the mulch treatment. However, the effect of mulch on these taxa as well as on the staphylinid P. fuscipennis likely was indirect rather than through predation on decomposer prey as no maize-borne carbon was detected in these taxa. Thomas et al. (2002) suggested that mobile species are able to respond rapidly to changing environmental conditions and food availability, and different soil textures likely influence oviposition, larval development and survival. Furthermore, enhanced prey availability triggers predator aggregation (Niemela et al. 1986; Kielty et al. 1996; Bohan et al. 2000), and our mulch application presumably increased prey species, as maize carbon was used as additional resource by decomposer soil invertebrates. Therefore, enhanced densities in larvae of staphylinid and carabid beetles in our mulch treatment indicate that these taxa had been attracted to these sites and may have contributed to the observed reduction in aphid populations. Addition of mulch may therefore also enhance the control of pest species by attracting predators; once attracted, they may contribute to pest control without benefiting from prey out of the decomposer system. In fact, generalist predators in arable fields such as carabid beetles are highly mobile and shift habitats from one generation to the next (Thomas et al. 1998).

Despite the apparently minor effect of maize mulch on predator densities, aphid numbers were significantly reduced by generalist predators in the mulch treatment. Reducing aphid numbers may have been due to generalist predators climbing the plants and also by feeding on aphids dislocated by rain onto the soil surface (von Berg et al. 2009). As only a few predator species were affected by maize mulch, control of aphid populations were likely due to these predators, i.e. P. fuscipennis, O. inustus and T. quadristriatus, as well as to carabid and staphylinid larvae. Interestingly, effects of maize mulch on densities of these predators differed significantly between fields. Moreover, only in two (field 1 and 3) out of the three investigated fields, aphid populations were significantly decreased by generalist predators. Lack of aphid control in field 2 may have been due to less pronounced colonisation of this field by P. fuscipennis from adjacent fields as field 2 was closer to the city of Göttingen with fewer fields in its surroundings as compared to fields 1 and 3.

By combining the data of field-specific effects of mulch on predator densities and predator effects on aphid populations, single predator species can be identified as most effective control agents. With significant predator effects on aphid populations in mulch plots in field 1 and simultaneously increased densities of the staphylinid P. fuscipennis exclusively in mulch plots in this field, this species most likely contributed to the significant aphid suppression in this field. Using molecular gut content analyses, P. fuscipennis is known to consume aphids at high rates (K. von Berg, unpublished data). Furthermore, this species may effectively control aphid populations as indicated in previous field studies (Sopp and Wratten 1986; Dennis and Wratten 1991). In the other field (field 3) where soil dwelling predators effectively suppressed aphid populations in mulch plots (−80%), densities of P. fuscipennis were negligible. In this field, densities of T. quadristriatus and O. inustus likely contributed to aphid control, as their densities were significantly increased in the mulch plots. T. quadristriatus preys on aphids (Sunderland et al. 1987; Mundy et al. 2000), and O. inustus, a species which is very common in agricultural fields (Krooss and Schaefer 1998; Markgraf and Basedow 2002), is carnivorous (Eghtedar 1970). Interestingly, densities of these two species were also increased in field 2; however, no effects on aphid populations were observed. Failure in aphid suppression has been recorded in some field studies (Holland et al. 1996; Holland and Thomas 1997; Collins et al.2002). Generally, aphid control is assumed to be most effective early in the season (Edwards et al. 1979; Chiverton 1986) and densities of T. quadristriatus in field 2 were most increased at the second and third sampling dates when aphid populations may have escaped predator control. The effect of carabid and staphylinid larvae is difficult to predict as they were not determined to species level and likely constitute of a number of species. Several carabid and staphylinid larvae have been documented to prey on aphids (Sunderland et al. 1987; Dennis et al. 1991; Kollat-Palenga and Basedow 2000; Kyneb and Toft 2004) and, therefore, the increased density of these larvae likely contributed to aphid suppression in our study.

Conclusions

The present study demonstrated the incorporation of detrital food resources (maize chaff) into an agricultural soil food web. Certain predator species of the aboveground system incorporated maize-borne carbon via prey out of the decomposer system thereby significantly increasing in density in the mulch treatment. Effects of the added detritus propagated via generalist predators into the herbivore system, as predators significantly decreased aphid populations in mulch fields. Predator and herbivore densities as well as effects of mulch addition varied strongly between fields emphasising the necessity to investigate multitrophic interactions in a landscape context. Further, the results suggest that single generalist predator species can significantly contribute to herbivore suppression in agricultural systems, and these effects may be fostered by residue management practices. Knowledge on the factors driving the population dynamics of these species therefore allows development of management practices which improve conservation biological control.

Acknowledgments

We are indebted to R. Langel (University of Göttingen) for stable isotope measurements. This study was supported by the German Science Foundation (DFG).

Open Access This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

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