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From:
Published online 2010 May 28. doi: 10.1186/1471-2105-11-287

Figure 6

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Colonyzer corrects spatial lighting gradients. Horizontal and vertical intensity slices from a 384 well plate image with growing S. cerevisiae spots captured on an S&P robotics [4] BM3-SC. Local intensity peaks represent cultures (at low cell density) or plate edges. Blue curves show the lighting-induced intensity gradient before correction. Red curves show a flat intensity gradient after correction, which maintains culture information.

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