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Epidermal growth factor receptor (EGFR) is a ubiquitously expressed receptor tyrosine kinase involved in the etiology of several human cancers. Cetuximab is an EGFR blocking-antibody that has been approved for the treatment of patients with cancers of the head and neck (HNSCC) and metastatic colorectal cancer (mCRC). Previous reports have shown that EGFR translocation to the nucleus is associated with cell proliferation. Here we investigated mechanisms of acquired resistance to cetuximab using a model derived from the non-small cell lung cancer line H226. We demonstrated that cetuximab-resistant cells overexpress HER family ligands including epidermal growth factor (EGF), amphiregulin (AR), heparin-binding EGF (HB-EGF) and β-cellulin. Overexpression of these ligands is associated with the nuclear translocation of the EGFR and this process was mediated by the Src family kinases (SFK). Treatment of cetuximab-resistant cells with the SFK inhibitor, dasatinib, resulted in loss of nuclear EGFR, increased membrane expression of the EGFR and re-sensitization to cetuximab. In addition, expression of a nuclear localization sequence tagged EGFR in cetuximab-sensitive cells increased resistance to cetuximab both in vitro and in mouse xenografts. Collectively, these data suggest that nuclear expression of EGFR may be an important molecular determinant of resistance to cetuximab therapy and provides a rationale for investigating nuclear EGFR as a biomarker for cetuximab response. Further, these data suggest a rationale for the design of clinical trials that examine the value of treating patients with cetuximab-resistant tumors with inhibitors of SFKs in combination with cetuximab.
The epidermal growth factor receptor (EGFR) is a ubiquitously expressed receptor tyrosine kinase (RTK) that is important in oncogenesis. Ligand activation of the EGFR leads to downstream signaling of several pro-survival cascades, such as the RAS/RAF/MEK/ERK/MAPK and PI(3)K/Akt pathways. Activation of the EGFR ultimately promotes cell proliferation, angiogenesis, invasion, metastasis, and survival making it one of the most promising molecular targets in cancer therapy (Arteaga, 2003; Bianco et al., 2005; Camp et al., 2005; Gupta et al., 2002; Kobayashi et al., 2005; Pao et al., 2005; Viloria-Petit and Kerbel, 2004; Yarden and Sliwkowski, 2001).
EGFR has been an actively investigated target over the last decade with five FDA agents approved in oncology since 2003. Two distinct molecular approaches to EGFR inhibition have emerged: 1) monoclonal antibodies (mAbs) directed against the ligand-binding site of the EGFR and 2) tyrosine kinase inhibitors directed against the intracellular tyrosine kinase domain (TKD). Although both strategies of inhibition have demonstrated promising anti-tumor activity in patients with metastatic colorectal cancer (mCRC), non-small cell lung cancer (NSCLC), breast cancer, and head and neck squamous cell carcinoma (HNSCC), only ~10 to 20% of patients manifest major clinical responses. Response to these agents appears to be limited by intrinsic as well as acquired resistance (Arteaga, 2003; Bianco et al., 2005; Camp et al., 2005; Gupta et al., 2002; Kobayashi et al., 2005; Pao et al., 2005; Viloria-Petit and Kerbel, 2004). Several mechanisms of resistance to cetuximab have emerged including deregulated ubiquitination, amplified HER3 signals, increased transforming growth factor alpha (TGFα), and altered angiogenesis (Lu et al., 2007; Rajput et al., 2007; Viloria-Petit et al., 2001; Wheeler et al., 2008). Although each of these studies suggests rational mechanisms of intrinsic and/or acquired resistance, our detailed knowledge of EGFR inhibitor resistance remains relatively ill defined (Mukohara et al., 2005).
It has been reported that EGFR family members can be shuttled from the plasma membrane to the nucleus (Giri et al., 2005; Liao and Carpenter, 2007; Lin et al., 2001; Lo et al., 2005a; Lo et al., 2006b; Lo and Hung, 2006; Massie and Mills, 2006; Ni et al., 2001; Offterdinger et al., 2002; Wang et al., 2004; Wang et al., 2006; Xie and Hung, 1994). Nuclear EGFR (nEGFR) has been demonstrated in highly proliferative tissues and linked with poor clinical outcome in breast, oropharyngeal squamous cell carcinoma, and ovarian cancer (Lo and Hung, 2006; Psyrri et al., 2005; Xia et al., 2008). Nuclear EGFR has two identified functions in the nucleus, 1) as a transcription factor and 2) in the direct phosphorylation of proliferating cell nuclear antigen (PCNA). As a transcription factor, EGFR has been shown to interact with STAT3 and E2F1 to mediate transcription of cyclin D1, iNOS, B-Myb and Aurora Kinase A (Cao et al., 1995; Hanada et al., 2006; Hung et al., 2008; Lin et al., 2001; Lo et al., 2005a; Lo et al., 2006b; Lo and Hung, 2006; Marti et al., 1991). In addition to transcription-regulated events, the EGFR has been shown to phosphorylate PCNA on tyrosine 211, thus stabilizing chromatin bound PCNA. This phosphorylation event correlates with pronounced cell proliferation (Wang et al., 2006).
In this study we investigated the role of nuclear EGFR in the resistance to cetuximab therapy. We showed that cells with acquired resistance to cetuximab have increased Src Family Kinase (SFK) activity, and this is linked to the translocation of the EGFR to the nucleus. Collectively, our findings reported herein suggest that nuclear expression of EGFR may be a critical determinant for resistance to cetuximab therapy and provides a rationale for designing clinical trials to examine the value of treating patients with resistance to cetuximab with inhibitors of SFKs in combination with cetuximab.
We have previously described the development and characterization of NCI-H226 NSCLC lines with acquired resistance to cetuximab (Wheeler et al., 2008). The development of this model resulted in six stable cetuximab-resistant clones for the H226 NSCLC line: HC1, HC4, HC5, HC6, HC7 and HC8. The sensitive parental line was designated HP. Three of these clones (HC1, HC4 and HC8) were tested for their sensitivity to cetuximab relative to the parental controls (Figure 1A). All clones displayed a robust cetuximab-resistant phenotype when challenged with 100 nM of cetuximab as compared to parental controls.
Nuclear expression of EGFR has been inversely correlated with survival in breast, oropharyngeal and ovarian cancer (Lo et al., 2005b; Psyrri et al., 2005). To build upon these published results, we investigated nEGFR expression in cetuximab-resistant cells relative to the parental controls to determine if nEGFR can function as a mechanism of escape to cetuximab therapy. We analyzed the cytoplasmic and nuclear distribution of EGFR in cetuximab-resistant cells. Figure 1B shows all three cetuximab-resistant clones had a dramatic increase in nuclear expression of the EGFR relative to the parental controls. We next utilized confocal microscopy to confirm nEGFR localization in cetuximab-resistant clones. These findings showed robust EGFR expression in all three clones in the nucleus, perinuclear membranes and cytosol (Figure 1C). Quantification of the cytoplasmic and nuclear fractions of each cetuximab-resistant clone, relative to parental controls, is represented in Figure 1D and indicated a ~1.5-fold increase in cytosolic expression and a ~2 to 2.5-fold increase in nuclear expression.
Nuclear EGFR has been shown to be associated with the transcription of cell cycle genes (cyclin D1, B-myb and Aurora kinase A), DNA repair, and DNA synthesis activity (PCNA phosphorylation and stability) (Bandyopadhyay et al., 1998; Dittmann et al., 2005; Friedmann et al., 2006; Hung et al., 2008; Lin et al., 2001; Lo et al., 2005a; Lo et al., 2006b; Wang et al., 2006). To confirm that the nEGFR observed in the cetuximab-resistant clones had nuclear function, we investigated the level of expression of these proteins in cetuximab-resistant clones relative to the parental controls. These experiments revealed that Cyclin D1, B-myb and PCNA were increased in their expression (Figure 1E). Collectively, these data indicate that cells with acquired resistance to cetuximab have increased expression of nEGFR, Cyclin D1, B-myb and PCNA.
It has been clearly demonstrated that EGF stimulates translocation of the EGFR to the nucleus (Hsu and Hung, 2007). We therefore, hypothesized that cetuximab-resistant cells may have increased expression of EGFR ligands leading to chronic EGFR translocation to the nucleus. We performed DNA microarray analysis comparing HP parental cells versus the HC4 cetuximab-resistant clone. Upregulation of several HER family ligands including EGF (~20-fold), heparin-binding EGF (HB-EGF, ~12-fold), amphiregulin (AR, ~20-fold) and β-cellulin (~3-fold) were identified in this microarray analysis (data not shown). To extend these findings to all resistant clones we performed real-time quantitative PCR to validate EGF, HB-EGF, AR and β-cellulin gene expression in passage controlled cetuximab resistant clones HC1, HC4 and HC8 relative to parental control (Figure 2A). Figures 2A-2D represent several functional assays to confirm the importance of EGFR ligands in the translocation of EGFR to the nucleus. To determine which of these ligands had the potential to drive the EGFR into the nucleus, we treated HP parental cells (low nEGFR expression) with purified ligands (200 ng/mL) and measured nEGFR. Each ligand (EGF, HB-EGF, AR, β-cellulin) induced nuclear translocation of the EGFR to varying degrees in HP parental cells (Figure 2A).
We expanded on these findings to determine if conditioned media from the cetuximab-resistant clones (HC1, HC4, and HC8) could induce nuclear translocation of the EGFR in HP parental cells (low nEGFR expression). Conditioned media from HP, HC1, HC4 and HC8 were collected after 72 hours and were incubated with HP cells that have been serum starved for 24 hours. Nuclear proteins were isolated 1 hour after the addition of the conditioned media and fractionated by SDS-PAGE. These results showed that conditioned media from the cetuximab-resistant clones, but not from the HP parental cells, could induce EGFR translocation to the nucleus (Figure 2B).
EGFR ligands are released from the cytoplasmic membrane via activation of tumor necrosis factor converting enzyme, TACE (Borrell-Pagès et al., 2003; Sunnarborg et al., 2002). We used the TACE inhibitor, TAPI2, to block the release of EGFR ligands in cetuximab-resistant cells as well as the parental control and measured the effect on nuclear translocation of the EGFR (Figure 2B). The results of these experiments indicated an approximate 3 to 5-fold decrease in nEGFR expression in cetuximab-resistant clones in the TAPI2 treated groups. These data suggest that blockade of TACE and thus release of growth factors results in decreased nuclear translocation of the EGFR.
In Figure 2C HP, HC1, HC4, and HC8 were serum starved for 3, 6, 12, and 24 hours and nEGFR translocation was then assessed. All cetuximab-resistant clones (HC1, HC4 and HC8), but not the parental HP, had increased nuclear translocation of the EGFR as time increased. These results suggest that factors from the cetuximab-resistant cells are released and lead to nuclear translocation of the EGFR over time.
If cetuximab-resistant cells have increased EGFR ligands and these ligands appear to drive nEGFR, we investigated whether or not this process would result in resistance to cetuximab. Growth proliferation assays indicated that the addition of ligand results in robust resistance to cetuximab (Figure 2D). Interestingly, HB-EGF and β-cellulin, which induced the most pronounced nEGFR translocation, resulted in the largest increase in resistance to cetuximab (Figure 2A and 2D).
We have previously shown that cells with acquired resistance to cetuximab have increased SFK activity relative to parental control (Wheeler et al., 2009). We hypothesized that ligand-induced EGFR translocation to the nucleus may be mediated by SFK. Pretreatment of the HP parental cells with the SFK inhibitor, dasatinib for 24 hours prior to ligand stimulation completely blocked EGFR translocation to the nucleus by these ligands (Figure 3A).
Since cetuximab-resistant cells have increased expression of EGFR ligands, SFK activity and nEGFR, we designed experiments to determine if these were linked. We investigated whether or not SFK blockade using dasatinib could reduce nEGFR expression in the cetuximab-resistant clones HC1, HC4 and HC8. Cetuximab-resistant clones were treated with vehicle or dasatinib for 24 hours; the cells were then fractionated for the cEGFR or nEGFR. Dasatinib treatments lead to a 2.7 to 5.5-fold decrease in nEGFR expression in the cetuximab-resistant clones (Figure 3B). The results of this experiment indicated that cetuximab-resistant cells have increased release of EGFR ligands, which then induce the translocation of the EGFR to the nucleus and this process is dependent upon SFK.
It has been well characterized that membrane-bound EGFR translocates from the membrane to the nucleus (Hsu and Hung, 2007; Lin et al., 2001; Lo et al., 2006a). We postulated that SFK blockade inhibited this process and would result with higher membrane levels in cetuximab-resistant clones after dasatinib treatment. To test this hypothesis, we treated HP, HC1, HC4 and HC8 with 50 nM dasatinib for 24 hours and performed flow cytometric analysis on membrane EGFR expression (Figure 3C). HP showed a decrease in membrane EGFR levels whereas all three cetuximab-resistant clones showed an increase in membrane expression of the EGFR after dasatinib treatment.
With an increase in EGFR membrane expression and a decrease in nuclear expression after dasatinib treatment, we asked the question of whether or not cetuximab-resistant clones would be more sensitive to cetuximab therapy. We performed proliferation analysis assays using DMSO control, 100 nM cetuximab, 25 nM dasatinib or the combination on HP, HC1, HC4 and HC8. The results of these experiments showed that dasatinib induced mild growth inhibition on cetuximab-resistant cells but the combination of the two drugs showed augmentation of growth inhibition (Figure 3D). These results suggest that blockade of SFK can block nuclear translocation of the EGFR, increase membrane EGFR and thus make cells more sensitive to cetuximab therapy.
To isolate the effects of nEGFR from ligand-induced nuclear translocation of EGFR, we designed a retrovirus expressing wild-type human EGFR fused to a nuclear localization sequence tag (NLS), followed by a myc tag for identification of the transgene (EGFR-NLS/Myc, Figure 4A). We infected cetuximab-sensitive parental line, HP, with EGFR-NLS/Myc or vector only. From these experiments we obtained three EGFR-NLS/Myc-expressing clones designated C4, C5, C10 and one vector only clone, V0. Figure 4A shows the characterization of these three clones. The nuclear fractions were purified and immunoprecipitated with a myc antibody. All clones were positive for the myc tag, EGFR and phospho-tyrosine (p-Tyr) of the EGFR, indicating active EGFR by Western blot analysis. These results were followed by immunofluorescence (IF) analysis of EGFR expression in the vector only and three EGFR-NLS/Myc-expressing clones. Each EGFR-NLS/Myc-expressing clone had robust EGFR expression in the nucleus and peri-nuclear membrane as well as cytosolic expression. These results indicated that our transgene was expressed in the nuclear fraction and active as demonstrated by total phosphorylation of tyrosine residues on the EGFR.
It has been reported that EGFR activates expression of cyclin D1 and B-myb (15, 31). To determine if the NLS-tagged EGFR had nuclear function, we measured the levels of cyclin D1 and B-myb. Figure 4B indicates that all EGFR-NLS/Myc-expressing clones have a 1.9 to 2.3-fold increase in expression of B-myb and a 1.4 to 1.6-fold increase in cyclin D1 expression. We utilized these clones with increased nEGFR expression and tested the hypothesis that this would result in increased resistance to cetuximab therapy. In Figure 4C we report that, relative to the parental HP controls, each clone exhibited an approximate 50% increase in resistance to cetuximab treatment. Collectively these data suggest that nuclear expression of the EGFR contributes to resistance to cetuximab therapy.
To examine whether these clones with nEGFR expression would be cetuximab-resistant in vivo we inoculated athymic nude mice with HP cetuximab-sensitive parental cells (HP), vector only (V0), and the three EGFR-NLS/Myc-expressing clones (C4, C5, and C10). When tumor volume reached 120-180 mm3 we began treatment with 0.1 mg IgG or 0.1 mg of cetuximab twice weekly. The results of this experiment indicate two major findings. First, tumor cells that express nEGFR grew approximately twice as fast relative to the HP or V0 controls. This is noted by in Figure 5. It took the HP and V0 cells approximately 27 days to reach 100 mm3, whereas all three EGFR-NLS/Myc-expressing clones reached the same tumor volume at approximately 12-13 days. This work was confirmed on a repeat experiment. Secondly, the three EGFR-NLS/Myc-expressing clones showed no statistical difference in growth to the IgG controls in response to cetuximab. Taken together, these results suggest that expression of an NLS-tagged EGFR can contribute to resistance to cetuximab in vivo.
H226 lines represent a NSCLC model of acquired resistance to cetuximab. However, intrinsic resistance to EGFR-targeted therapy is a significant clinical problem. We were interested to determine if other tumor cell lines with known responses to cetuximab in vitro could be correlated with nEGFR expression. To test this hypothesis, we utilized nine established HNSCC lines: SCC1, SCC4Y, SCC6, SCC11A, SCC14A, SCC17B, SCC22b, SCC37A and SCC1483. Cytoplasmic and nuclear protein from these cell lines were fractionated and blotted for EGFR (Figure 6A). Cells were treated with vehicle or 5nM of cetuximab for 72 hours and MTT assays performed (Figure 6B). The results of this experiment indicated that cells that have intrinsic resistance to cetuximab therapy, in vitro, have increased nEGFR (SCC4Y, SCC6, SCC11A, and SCC14A) and those with low nEGFR have a higher sensitivity to cetuximab treatment (SCC1, SCC17B, SCC37A and SCC1483). SCC22B was used as an EGFR negative expressing line, and thus was not responsive to cetuximab therapy.
Cetuximab has shown great promise in clinical oncology, but is limited by intrinsic as well as acquired resistance. These resistance issues present a clinical obstacle for the optimal advancement of this promising molecular targeting agent. In this study we investigated mechanisms of acquired resistance to cetuximab using previously established cetuximab-resistant tumor clones following long-term exposure to cetuximab (Wheeler et al., 2008). Sub-cellular analysis of EGFR localization in cetuximab-resistant clones indicated a marked increase in nuclear expression of the EGFR (Figure 1B-D). Emerging evidence over the last decade has indicated that nEGFR has been detected in highly proliferative tissues and linked with poor clinical outcome in breast, oropharyngeal SCC, and ovarian cancer (Lo and Hung, 2006; Psyrri et al., 2005; Xia et al., 2008). Furthermore, nEGFR functions as a transcription factor for cyclin D1, iNOS, B-myb and Aurora Kinase A (Cao et al., 1995; Hanada et al., 2006; Hung et al., 2008; Lin et al., 2001; Lo et al., 2005a; Lo et al., 2006b; Lo and Hung, 2006; Marti et al., 1991) as well as phosphorylating and stabilizing PCNA (Wang et al., 2006). These data suggest that nEGFR provides a “second compartment” of proliferation mediated by the EGFR, the first compartment being the classical membrane-bound EGFR signaling through the RAS/RAF/MEK/MAPK pathway. Analysis of cetuximab-resistant clones indicated a marked upregulation of cyclin D1, B-myb and PCNA demonstrating that nEGFR was functional in cetuximab-resistant clones. Together, these observations suggest a potential model of resistance to cetuximab therapy (Figure 7).
It has been reported that EGF stimulation can induce the accumulation of EGFR in the nucleus (Hsu and Hung, 2007). This finding led us to investigate the levels of ligand expression in cetuximab-resistant cells (Figure 2A). In addition to EGF, we found several EGFR ligands to be increased. Furthermore, we found that all ligands that were upregulated had the potential to induce translocation of the EGFR to the nucleus with varying degrees (Figure 2A). These data suggest that deregulation of EGFR ligands have the potential to drive EGFR to the nucleus. Furthermore, blockade of TACE, the enzyme necessary for release of EGFR ligands from the membrane (Borrell-Pagès et al., 2003; Sunnarborg, 2002; Sunnarborg et al., 2002), dramatically decreased nEGFR in all cetuximab-resistant clones, further strengthening a role for ligands in this process (Figure 2B). If EGFR ligands can drive nEGFR, then ligand alone should induce moderate levels of resistance to cetuximab. In Figure 2D we tested this hypothesis by adding individual ligands or ligand plus cetuximab and measured the resistance. Each ligand resulted in a varying degree of resistance but appeared that HB-EGF and β-cellulin, which induced the more accumulation of EGFR in the nucleus (Figure 2A), correlated with the most robust resistance (Figure 2D). Taken together these findings indicate that EGFR ligands may be a critical determinant of inducing resistance to cetuximab and may be a viable screening tool for therapeutic response to cetuximab. Similar findings have been reported for additional HER family ligands in the resistance to HER family inhibitor therapy (Rajput et al., 2007; Ritter et al., 2007). Collectively our results and these reports suggest that surveillance of HER family ligands levels may provide an indicator of therapeutic response to cetuximab and other HER family inhibitors.
Recent experiments have shown that EGF can induce nuclear translocation of the EGFR. In Figure 3A, we expanded on this finding and showed that EGF, HB-EGF, AR, and β-cellulin drive EGFR to the nucleus. Furthermore, we showed that dasatinib could block this translocation. These findings suggest that EGFR movement to the nucleus is dependent, in part, on SFK, and this may be an early signal in translocation. Dasatinib treatment of cetuximab-resistant clones led to a dramatic decrease in the nEGFR (Figure 3B). Taken together these findings suggest that overexpression of EGFR ligands initiate movement of the EGFR to the nucleus, mediated by SFK. Blockade of SFK may represent a unique therapeutic target to block nEGFR in patients.
Our results also imply that blockade of SFK led to increased EGFR on the plasma membrane and not the nucleus (Figure 3C). This is most likely due to the fact that SFK's are an initial signal for movement of the EGFR from the membrane to the nucleus. We postulated that increased membrane EGFR and loss of the “second compartment” of EGFR signaling in the nucleus after SFK blockade may restore sensitivity to cetuximab. This was seen in Figure 3D where the combinatorial therapy of dasatinib and cetuximab had greater impact than either agent alone. The overall findings from this series of experimentations indicate that targeting both the EGFR and SFK may provide a valuable clinical strategy to explore. Investigations into which Src family member may regulate EGFR translocation to the nucleus remain to be elucidated.
It is difficult to determine if nEGFR itself can drive resistance since the presence of ligand appears to be necessary. We took steps to distinguish these two processes by overexpressing EGFR tagged to a nuclear localization sequence (EGFR-NLS) in the cetuximab-sensitive HP parental lines in vitro and in vivo (Figure 4 and and5).5). Three different EGFR-NLS/Myc-expressing clones, was localized to the nucleus, was tyrosine phosphorylated and resulted in the upregulation of Cyclin D1 and B-myb (Figure 4A and B). Each EGFR-NLS/Myc-expressing clone had increases in resistance to cetuximab in vitro (Figure 4C). In addition, when these tumor cells were utilized in mouse xenograft models and challenged repeatedly with cetuximab, all three clones showed no significant difference in growth relative to the IgG control, whereas the HP and the vector only controls had statistically significant tumor growth control with cetuximab (Figure 5). Collectively these data support the hypothesis that nEGFR, in part, can play a role in resistance to cetuximab therapy.
Nuclear EGFR has been strongly correlated with poor overall survival in several tumor types (Lo et al., 2005b; Psyrri et al., 2008; Xia et al., 2008). In summary, this is the first report indicating that nEGFR may play a functional role in the response to molecular therapeutics agents. The findings reported herein have clinical implications for the design of optimal therapeutic strategies. In this model, we demonstrate that HER family ligands are upregulated in the cells with acquired-resistance to cetuximab. This in turn enhances the translocation of the EGFR to the nucleus. This process appears to be dependent on SFK. Once in the nucleus, EGFR performs several functions that mediate proliferation of tumor cells. In patients where this mechanism may be prominent, SFK inhibitor, dasatinib represents an approach inhibit nuclear translocation, leading to removal of nEGFR and its functions with re-sensitization to cetuximab therapy. Furthermore, these findings suggest that nEGFR may prove a viable molecular target. This may be undertaken by targeting nEGFR-expressing tumors with EGFR TKI (erlotinib and gefitinib) as they can diffuse across the membrane and abrogate nEGFR kinase function such as phosphorylation of PCNA. Strategies along these lines have already been suggested (Wheeler et al., 2008). In addition, targeting with dasatinib and cetuximab either concomitantly or sequentially may be a viable clinical approach for individuals with nuclear expression of EGFR.
The human NSCLC line NCI-H226 was purchased from ATCC (Manassas, VA) and maintained in 10% fetal bovine serum in RPMI1640 (Mediatech Inc., Manassas, VA) with 1% penicillin and streptomycin. The development of cells with acquired resistance has been previously described (Wheeler et al., 2008). UM-SCC1, UM-SCC4Y, UM-SCC6, UM-SCC11A, UM-SCC14A, UM-SCC17B, UM-SCC-22B, and UM-SCC1483 cells were obtained from University of Michigan and University of Pittsburgh School of Medicine provided PCI-37a (SCC37A) cells. The cells were maintained in 10% fetal bovine serum (FBS, Invitrogen, Carlsbad, CA) in Dulbecco's modified Eagle's medium (DMEM, Mediatech Inc.) supplemented with 1% hydrocortisone.
Cetuximab (C225, Erbitux™) was provided by ImClone Systems Inc., (New York, NY). Dasatinib (BMS-354825, Sprycel™) was provided by Bristol-Meyers Squibb (New York, NY). EGF, HB-EGF, human amphiregulin (AR) and β-cellulin were purchased from R&D Systems (Minneapolis, MN). TAPI2 was purchased from Peptide International (Louisville, KY). All other chemicals were purchased from Sigma (St. Louis, MO).
Wild-type human EGFR cDNA was subcloned into the NcoI/NotI restriction sites of the pCMV/myc/nuc vector to create the EGFR-NLS/myc construct (Invitrogen). This was then amplified via the polymerase chain reaction and subcloned into the pQCXIP retroviral vector (Clontech, Mountain View, CA). Virus was generated according to the manufacturer's recommendations. Infected cells were grown in RPMI1640 medium containing puromycin (2 μg/mL). Stable clones were selected and maintained in RPMI1640 with 0.5 μg/mL of puromycin.
Exponentially grown cells were seeded in 6-well plates. Following treatment, monolayers were washed with PBS and fixed/stained with 0.5% crystal violet. Plates were air dried overnight and dye was eluted with 0.1 M sodium citrate (pH 4.2) in ethanol (1:1). Elution was transferred to 96-well plates, and the absorbance was read at 540 nm to determine cell growth. The percentage cell growth was calculated by comparison of the A540 reading from treated versus control cells. The anti-proliferative effect of cetuximab (Figure 6B) was evaluated using MTT assay (12, 34(Wheeler et al., 2009)
Whole cell protein lysate was obtained with lysis buffer (Wheeler et al., 2008). Cellular fractionation was performed as described previously (Hsu and Hung, 2007). Protein was quantitated using the Bradford method (Bio-Rad Laboratories, Hercules, CA). Western blotting was performed as described previously (Wheeler et al., 2008). The NIH ImageJ program was used to measure densitometry of the Western bands. The antibodies used in this study were as follows: EGFR, p-EGFR (Tyr1173), CyclinD1, B-Myb, HRP-conjugated goat-anti-rabbit IgG and goat-anti-mouse IgG were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). PCNA, PARP and histone H3 were obtained from Cell Signaling Technology (Beverly, MA). Phospho-tyrosine (4G10) was purchased from Millipore (Billerica, MA), alpha-tubulin from Calbiochem (San Diego, CA), and c-Myc from Invitrogen.
Nuclear lysates containing 0.2 mg of protein were incubated overnight with 2 μg of anti-Myc antibody. After adding 30 μl of protein A/G agarose beads, nuclear lysates were incubated for another 2 hours at 4°C. The immunoprecipitates were pelleted by centrifugation and washed three times with lysis buffer and three times with PBS. The captured immunocomplexes were then eluted by boiling the beads in 2× SDS sample buffer for 5 minutes and subjected to immunoblot analysis.
Cells were washed in PBS and harvested with trypsin-EDTA (0.05% solution, Gibco, Carlsbad, CA). A cell suspension containing 1 × 106 cells in 100 μL staining buffer (SB-PBS containing 1% BSA) was incubated with 2 μg of either control IgG or anti-EGFR FITC-conjugated antibodies (Santa Cruz Biotechnology Inc.) for 1h at 4°C. Cells were washed and resuspended in 300μl of SB. Propidium iodide (PI) at final concentration of 5 μg/ml was added to each sample just prior to analysis on the cytometer. Samples were analyzed on a FACSCalibur flow cytometer (BD biosciences, San Jose, CA) and a minimum of 20,000 events per sample was acquired. Histogram analysis was performed with FlowJo software (Tree Star, Inc., Ashland, OR). Data was restricted to live events based on PI exclusion.
Approximately 2 × 103 cells were seeded on a four-well glass chamber slide (Nalgene Nunc, Naperville, IL) overnight. Forty-eight hours later, cells were washed 3 times with PBS and fixed with 2% formaldehyde for 15 minutes at room temperature. Cells were incubated in ice-cold 100% methanol for 10 minutes at -20°C and blocked with 5% normal serum in PBS with 0.3% Triton X100 solution for 1 hour at room temperature and incubated with anti-EGFR antibody overnight at 4°C. Next, cells were incubated with FITC-conjugated appropriate secondary antibody in PBS containing 0.3% Triton X100 and 1% BSA for 2 hours. Slides were mounted using ProLong gold with DAPI (Invitrogen). Fluorescence microscopy, photography and measurement intensity were carried out using BD Pathway 855 (BD Biosciences).
Total RNA was isolated using the TRIzol reagent (Life Technologies, Gaithersburg, MD). cDNAs from total RNA of HP, HC1, HC4 and HC8 were synthesized using Superscript III First-Strand Synthesis System (Invitrogen Corp, Carlsbad, CA). Real-time PCR analysis was performed using a Bio-Rad iQ5 Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA) using the iQ Supermix as recommended by manufacturer. All reactions were performed in triplicate. The sequences of the primer sets used for this analysis are as follows: EGF (Fw) 5′-AGCTAACCCATTATGGCAACA-3′, (Rv) 5′-AGTTTTCACTGAGTCAGCTCCAT-3′, and (Probe) 5′-FAM-AGGGCCCTGGACCCACCAC-TAMRA-p-3′; amphiregulin (AR) (Fw) 5′-ATATCACATTGGAGTCACTGCCCA-3′, (Rv) 5′-GGGTCCATTGTCTTATGATCCAC-3′, and (Probe) 5′-FAM-AGCCATAAATGATGAGTCGGTCCTCTTTCC-TAMIRA-p-3′; heparin-binding EGF (HB-EGF) (Fw) 5′-GAAAGACTTCCATCTAGTCACAAAGA-3′, (Rv) 5′-GGGAGGCCCAATCCTAGA-3′, and (Probe) 5′-FAM-TCCTTCGTCCCCAGTTGCCG-TAMRA-p-3′; beta-cellulin (BTC) (Fw) 5′-TGCCCCAAGCAATACAAGC-3′, (Rv) 5′-CGTCTGCTCGGCCACC-3′, and (Probe) 5′-FAM-AAGCGGCATCTCCCTTTGATGCAGTAA-TAMRA-p-3′. Fold increases or decreases in gene expression were determined by quantitation of cDNA from target samples (HC1, HC4 and HC8) relative to a calibrator sample (HP). Human beta-actin gene was used as the endogenous control for normalization of initial RNA levels. To determine this normalized value, 2-ΔΔCT values were compared between target and calibrator samples, where the change in crossing threshold (ΔCt)= Ct target gene − Ct GAPDH and ΔΔCt = ΔCt HP − ΔCt HC1, HC4 or HC8.
Athymic nude mice (4 to 6-week-old males) were obtained from the Harlan laboratories (Indianapolis, IN). All animal procedures and maintenance were conducted in accordance with the institutional guidelines of the University of Wisconsin. Cetuximab-sensitive parental cells (HP) vector only control, or CMV-EGFR-NLS/Myc clones (C4, C5 and C10) were injected bilaterally in the dorsal flanks of the mice at day 0 (2 × 106 cells). Once tumors reached volumes 120-180 mm3 eight mice per group were randomized to IgG or cetuximab. Mice received 0.1 mg intraperitoneal cetuximab or IgG injections twice per week. Tumor volume measurements were evaluated by digital calipers and calculated by the formula (π)/6 × (large diameter) × (small diameter)2.
Student t-test was used to evaluate the significance of change in percent growth and EGFR cell surface expression. Statistical analysis to compare tumor sizes in xenograft-bearing mice was also done with a t-test. Differences between clones were considered statistically significant if P≤0.05.
We would like to thank Shyhmin Huang, Eric A. Armstrong, and Sergio Benavente for their initial work in the establishment of cetuximab-resistant H226 cell lines. Cetuximab and dasatinib were kindly provided by ImClone and Bristol Myers Squibb, respectively.
Conflict of interest: The authors declare no conflict of interest.