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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurosci. Author manuscript; available in PMC 2010 December 9.
Published in final edited form as:
PMCID: PMC2900154
NIHMSID: NIHMS212069

Afferent-specific AMPA receptor subunit composition and regulation of synaptic plasticity in midbrain dopamine neurons by abused drugs

Abstract

Ventral tegmental area (VTA) dopamine (DA) neurons play a pivotal role in processing reward-related information and are involved in drug addiction and mental illness in humans. Information is conveyed to the VTA in large part by glutamatergic afferents that arise in various brain nuclei, including the pedunculopontine nucleus (PPN). Using a unique rat brain slice preparation, we found that PPN stimulation activates afferents targeting GluR2-containing AMPA receptors (AMPAR) on VTA DA neurons, and these afferents did not exhibit long-term depression (LTD). In contrast, activation of glutamate afferents onto the same DA neurons via stimulation within the VTA evoked EPSCs mediated by GluR2-lacking AMPARs that demonstrated LTD or EPSCs mediated by GluR2-containing AMPA receptors that did not express LTD. Twenty four hours after single cocaine injections to rats, GluR2-lacking AMPARs were increased at both PPN and local VTA projections, and this permitted LTD expression in both pathways. Single injections with the main psychoactive ingredient of marijuana, delta-9-tetrahydrocannabinol (Δ9-THC), increased GluR2-lacking AMPA receptors and permitted LTD in only the PPN pathway, and these effects were prevented by in vivo pretreatment with the cannabinoid CB1 receptor antagonist AM251. These results demonstrate that cocaine more globally increases GluR2-lacking AMPA receptors at all glutamate synapses on VTA dopamine neurons, whereas Δ9-THC selectively increased GluR2-lacking AMPA receptors at subcortical PPN synapses. This suggests that different abused drugs may exert influence over distinct sets of glutamatergic afferents to VTA DA neurons which may be associated with different reinforcing or addictive properties of these drugs.

Keywords: cocaine, marijuana, Δ9-THC, cannabinoid, synaptic plasticity, LTD

VTA DA neurons play a pivotal role in processing reward-relevant information and are involved in addiction and mental illness in humans (Wise, 2005). Environmental stimuli can cause DA neurons to shift firing patterns from pacemaker to high frequency bursts of activity, leading to large increases in DA release in target areas (Gonon, 1988; Schultz, 1998; Hyland et al., 2002). Glutamate activation of ionotropic AMPARs on DA neurons appears to play an important role in this shift to bursting (Overton and Clark, 1997; Kitai et al., 1999; Georges and Aston-Jones, 2002; Floresco et al., 2003). Acute and long-term exposure to abused drugs can alter VTA glutamatergic afferents and produce enduring changes in DA neuron activity (Wolf et al., 2004). Thus, these VTA afferents exhibit changes, such as long-term potentiation (LTP) or LTD after exposure to abused drugs in vivo, and following local electrical stimulation in vitro (Bonci and Malenka, 1999; Jones et al., 2000; Mansvelder and McGehee, 2000; Ungless et al., 2001; Saal et al., 2003; Bellone and Luscher, 2005; Kauer and Malenka, 2007).

Although synaptic plasticity represents an intriguing substrate to explain lasting effects of abused drugs on reward circuits, there remain unresolved issues relating to this phenomenon. The VTA receives glutamatergic inputs from diverse brain nuclei (Geisler et al., 2007), and the origin of the afferents undergoing plasticity in vitro remain largely unknown. This is because most nuclei sending afferents to the VTA are not present within conventional brain slice preparations, and because many afferents are severed during the slicing procedure. Although cortical afferents provide input to the VTA, major glutamatergic afferents originating in subcortical nuclei also provide substantial direct input to VTA DA neurons (Omelchenko and Sesack, 2007). The PPN is one subcortical nucleus providing ascending glutamatergic, GABAergic, and cholinergic input to VTA DA neurons (Cornwall et al., 1990; Oakman et al., 1995; Geisler and Zahm, 2005; Wang and Morales, 2009). Consistent with its projections to the VTA, PPN stimulation increases DA neuron activity and DA concentrations in projection areas (Klitenick and Kalivas, 1994; Floresco et al., 2003), suggesting that it influences reward and motivational functions of the VTA. This is supported by studies showing that PPN lesions disrupt learned associations between primary rewards and neutral stimuli (Inglis et al., 2000), conditioned-place preference to opiates and psychostimulants (Bechara and van der Kooy, 1989; Olmstead and Franklin, 1993), amphetamine-induced sensitization (Nelson et al., 2007), and self-administration of heroin (Olmstead et al., 1998).

Despite its potential importance in mediating the effects of abused drugs, the PPN inputs to the VTA are poorly characterized. To address this, we developed a brain slice preparation that preserves PPN neurons and projections to VTA DA cells, and demonstrated that PPN-activated glutamate synapses could be studied independently of those activated by intra-VTA stimulation (Good and Lupica, 2009). We utilized this preparation in the present study to examine distinct glutamatergic inputs to VTA DA neurons, determined their susceptibility to LTD, and whether these properties are altered by cocaine and the psychoactive constituent of marijuana, Δ9-THC.

Materials and Methods

All animal procedures were conducted under National Institutes Health Guidelines using the NIH Guide for Animal Care and Use, which is based upon the United States Animal Welfare Act. The protocols were approved by the Institutional Animal Care and Use Committee (National Institute on Drug Abuse, Intramural Research Program, Baltimore, MD), which is accredited by the International Association for Assessment and Accreditation of Laboratory Animal Care.

Brain Slice Preparation

Parasagittal brain slices containing the PPN and the VTA were prepared as previously described (Good and Lupica, 2009). Briefly, juvenile (14–19 day old) rats were removed from a shared home cage no more than 15 minutes prior to decapitation. Animals were decapitated using a guillotine and the brain subsequently removed from the cranium and immediately transferred to a beaker containing oxygenated (95% O2/5% CO2), ice-cold (2–4° C), artificial cerebral spinal fluid (aCSF) of the following composition (mM): sucrose, 194; NaCl, 30; KCl, 4.5; MgCl2, 1; NaH2PO4, 1.2; glucose, 10; NaHCO3, 26; ascorbic acid, 0.4. The brains were then blocked for slicing as described previously (Good and Lupica, 2009). The remaining block of tissue was glued onto the cutting stage of a vibrating tissue slicer (Leica, VT1000) and submersed in ice-cold, oxygenated aCSF. The brain was positioned with the right hemisphere down and the thalamus facing the cutting blade. Two slices (280 μm) containing both the VTA and PPN were obtained from each rat using this procedure. The slices were transferred to an oxygenated holding chamber (31° C) and allowed to recover for at least 1 hour before recording.

Electrophysiology

One brain slice was transferred to a heated recording chamber (31 – 33°C) and superfused with aCSF (2 ml/min) containing (in mM): NaCl, 126; KCl, 3; MgCl2, 1.5; CaCl2, 2.4; NaH2PO4, 1.2; glucose, 11; NaHCO3, 26. Visualization of VTA neurons was performed with an upright microscope (Zeiss Axioscope, Germany), modified to provide a gradient-contrast image utilizing infrared illumination. Recording electrodes (3–5MΩ) contained (in mM): CsCl, 130; NaCl, 4; MgCl2, 2; EGTA, 1.1; HEPES, 5; Na2-ATP, 2; Na2-creatine-phosphate, 5; Na3-GTP, 0.6; spermine, 0.1; bioctytin, 0.125%; pH = 7.2 using KOH (osmolarity = 270–274 mOsm). Whole-cell voltage clamp recordings were performed using an Axopatch 200B (Axon Instruments, Burlingame, CA). Voltage steps and stimulation protocols were delivered using the Strathclyde electrophysiology software package (WCP, courtesy of Dr. John Dempster, Strathclyde University, Glasgow, UK; http://spider.science.strath.ac.uk/sipbs/software_ses.htm) and an A/D board (ITC-18, Instrutech Corp., Bellmore, NY, USA) residing in a personal computer.

Neurons were first identified visually, and then selected on the basis of a large (> 200 pA) hyperpolarization-activated inward current (Ih), as originally described by Johnson and North (1992). Although more recent evidence demonstrates that the presence of Ih is not an unequivocal marker of VTA DA neurons (Margolis et al., 2006), additional studies show that approximately 90% of tyrosine hydroxylase (TH) positive DA neurons in the VTA demonstrate large Ih, and only 13% of the Ih positive cells did not express TH (Cameron et al., 1997). Therefore, the absolute number of non-DAergic cells demonstrating large Ih in the VTA is likely low (Grace and Onn, 1989; Johnson and North, 1992). To further address this, dopamine transporter (DAT) immunohistochemistry was performed in most biocytin-filled VTA neurons to confirm the co-localization of large Ih magnitude with DAT immunohistochemistry (Fig. 1a). To measure Ih, neurons were voltage clamped at −60 mV and voltage steps from −70 to −110 mV were used.

Figure 1
Joro spider toxin (JTx) inhibition of EPSCs evoked via intra-VTA, but not PPN stimulation, indicates the absence or presence of the GluR2 AMPAR subunit, respectively, at these synapses on VTA DA neurons. (a) Photomicrograph of a biocytin-filled (left) ...

As previously described, synaptic currents were evoked using two bipolar tungsten electrodes (Frederick Haer, Bowdoinham, ME) with a tip separation of 300 μm; one placed on the posterior PPN and the other anterior to the VTA (Good and Lupica, 2009). After placing each of the stimulators, single pulses (0.2 msec duration) were alternated between the intra-VTA and PPN stimulators, allowing 45 seconds between each alternating stimulus, and 90 seconds between stimuli applied to the same pathway. In our previous study it was found that at interpulse intervals shorter than 90 sec there was significant rundown of the PPN-evoked synaptic current (Good and Lupica, 2009).

To calculate the rectification index (RI), full I/V curves relating EPSC amplitude to membrane voltage were generated at holding potentials between −80mV and +40mV, in 20 mV increments, with 3 responses arising from each pathway averaged at each holding potential. The peak mean EPSC amplitude obtained at −80 mV was then divided by the mean EPSC amplitude obtained at +40 mV. In figures in which the polyamines JTx or NASPM were used, or when LTD was measured, each point represents the mean +/− SEM of EPSCs recorded at each time point.

All LTD experiments were performed in the presence of picrotoxin, AP-5 and atropine to block GABAA, NMDA and muscarinic acetylcholine receptors, respectively. The synaptic currents remaining in AP-5 and picrotoxin were fully blocked by DNQX, indicating the involvement of AMPA receptors in these responses (data not shown).

Following 10 minutes of baseline recording, LTD was initiated by 5 trains of 5 pulses at 66Hz, delivered at 10 second intervals through one of the stimulating electrodes. If LTD was not observed after the first train of 66Hz stimuli, at least one more set of trains was initiated to ensure that mGluRs were sufficiently activated. LTD was then assessed by returning to the alternating stimulation of the PPN and intra-VTA pathways at 45 second intervals.

Drug injections

Sprague-Dawley rats (Charles River Laboratories, Raleigh, NC, USA) were injected i.p. with 15 mg/kg cocaine, 10 mg/kg THC, 2 mg/kg AM251 or 0.9% saline with a 28 G insulin syringe. Following these injections, each animal was immediately returned to its home cage. Injection volumes were small (100–200 μL) to minimize stress to the animal. AM251 was injected either 30 minutes prior to THC injection or alone.

Immunohistochemistry

The co-localization of biocytin and the DAT was performed in brain slices containing a single biocytin filled VTA neuron from which a recording was made. These slices were fixed in 4% paraformaldehyde overnight and then transferred to a phosphate buffered solution (PBS) containing 18% sucrose. The slices were then re-sectioned on a cryostat (20 μm), collected on slides, and washed in PBS (2 × 5 min). Sections were then incubated in a blocking buffer comprised of PBS containing 4% bovine serum albumin (BSA) and 0.3% Triton-X-100 for 1 h at room temperature, then transferred for 24 h to a solution (at 4° C) containing the DAT antibody (Millipore, Billerica, MA, USA), diluted 1:100 in blocking buffer. Sections were then washed twice in PBS, and incubated in Alexa Fluor 568-conjugated goat antibody to rat (Invitrogen, Carlsbad, CA, USA) for 2h at room temperature. To detect biocytin, sections were washed twice in PBS and incubated for 1h at room temperature in a streptavidin, Alexa Fluor 488 conjugate (Invitrogen, Carlsbad, CA, USA), diluted 1:800 in blocking buffer.

Data Analysis and Statistics

Data in text and figures are represented by mean ± SEM. Statistical analysis was performed using either a two-tailed Student’s t test or a one way ANOVA for multiple comparisons using GraphPad Prism v5.01 (GraphPad Scientific, San Diego, CA, USA). Z-tests were used to compare proportions of cells that underwent LTD in control and drug treatment conditions. In order to determine whether EPSCs from individual neurons were significantly affected by a particular manipulation (e.g. LTD-inducing stimuli) we calculated the SEM of all EPSCs elicited at each pathway in all DA neurons during the control period, prior to the initiation of any manipulation (equivalent to 10 minutes of baseline data for each neuron). This value was doubled (2 x SEM) and compared to the change in EPSC amplitude measured between 30 and 40 minutes following a particular manipulation. Any cell exhibiting a change in EPSC amplitude exceeding 2 x the control SEM for a given pathway (equivalent to a 10.2 % change for the intra-VTA pathway, and a 15% change for the PPN pathway) was then categorized as a “responder”, whereas EPSCs exhibiting no change, or a change in amplitude < 2 x control SEM was categorized as a “non-responder”. This provided a statistical measure to test the null hypothesis that there was no change in EPSC amplitude following a manipulation. This statistical test is equivalent to establishing a 95% confidence interval based on the mean variance of all cells during the baseline period because 95% of the data should fall within 2 standard errors of the mean, if there is no change in EPSC amplitude. Thus, assuming a normal distribution, any change in EPSC amplitude exceeding 2 x SEM is thought to be significantly different from the mean because the probability of this occurring by chance is ≤ 5%.

Drugs

Atropine, picrotoxin, Joro spider toxin, spermine, biocytin and NASPM were purchased from Sigma-Aldrich (St. Louis, MO, USA). 6,7-Dinitroquinoxaline-2,3-dione (DNQX) and 3,5 dihydroxyphenylglycine (DHPG) was supplied from Tocris (Ballwin, MO, USA). D-(−)-2-Amino-5-phosphonopentanoic acid (D-AP5) was purchased from Ascent Scientific (Princeton, New Jersey, USA). Cocaine and Δ9-THC were obtained from the NIDA drug supply system. JTx, NASPM, DNQX and DHPG were applied through bath superfusion using calibrated syringe pumps (Razel Scientific Instruments, Stamford, CT, USA).

Results

GluR subunit differences in distinct VTA DA neuron afferent synapses

Most AMPARs in the mature mammalian CNS are heteromeric tetramers, containing the subunits GluR1-GluR4 in varying combinations. AMPAR permeability to Ca2+ is governed by the presence or absence of the GluR2 subunit, such that AMPARs lacking GluR2 gate Ca2+, whereas those containing GluR2 are impermeable to Ca2+ (Isaac et al., 2007). The presence of GluR2 subunits in synaptic AMPARs can be inferred from the distinct biophysical properties of synaptic EPSCs and their sensitivity to polyamine channel blockers such as Joro spider toxin (JTx), and its synthetic analog 1-naphthylacetylspermine trihydrochloride (NASPM). Thus, EPSCs mediated by GluR2-containing AMPARs do not inwardly rectify and are insensitive to polyamines, whereas AMPARs lacking GluR2 (i.e. GluR1 homomers or GluR3/GluR1 heteromers) show inward rectification and are blocked by polyamines. The incorporation of GluR2 into AMPARs is regulated by synaptic activity, and the increase in Ca2+ permeability following its removal is involved in both transient and sustained changes in synaptic strength (Bellone and Luscher, 2005; Bellone and Luscher, 2006; Isaac et al., 2007; Mameli et al., 2007).

We previously demonstrated in parasagittal brain slices containing the PPN and VTA that brief electrical stimulation of these nuclei can elicit AMPAR-mediated EPSCs possessing distinct physiological and pharmacological characteristics in the same VTA DA neurons (Good and Lupica, 2009). However, the subunit composition of the AMPARs at these independent synapses were not determined. Therefore, we first examined the effect of the selective blockers of GluR2-lacking AMPARs, JTx (500 nM) or NASPM (25 μM) on EPSCs evoked via stimulation of the PPN or within the anterior VTA (intra-VTA) in the same VTA DA neurons (Fig. 1b, 1e). Whereas a subpopulation of intra-VTA evoked EPSCs were inhibited by JTx (n = 16/25, 64%), all EPSCs elicited through PPN stimulation were unaffected by this polyamine (n = 19, 0%). Thus, in the same VTA DA neurons, many glutamate synapses activated by intra-VTA stimulation lacked GluR2, whereas most or all PPN-activated glutamate afferents targeted AMPARs containing GluR2.

As a result of voltage-dependent blockade of GluR2-lacking AMPARs by endogenous polyamines, EPSCs mediated by these ion channels also demonstrate inward rectification at positive membrane potentials (Isaac et al., 2007). Thus, EPSCs recorded at +40 mV are smaller than those at −80 mV, and the ratio of these EPSCs, known as the rectification index (RI = mean peak EPSC−80mV/mean peak EPSC+40mV), can be used as a biophysical marker for the presence or absence of GluR2-containing AMPARs. Therefore, to confirm the results obtained with the polyamine blockers, we measured the RI of EPSCs activated through PPN or intra-VTA stimulation in the same VTA DA neurons before and after JTx application. For intra-VTA evoked EPSCs, the baseline RI was significantly larger than that observed after JTx application (control RI = 3.89 +/−0.52, n = 6; JTx RI = 2.08 +/− 0.28, n = 6; P < 0.01, paired t-test), whereas the RI for EPSCs evoked by PPN stimulation were unaffected by JTx (control RI = 3.1 +/− 0.56, n = 3; JTx RI = 2.67 +/− 1.1, n = 3; p = 0.55, paired t-test; Fig. 1c, 1d). No change was seen in RI before or after JTx application in the remaining intra-VTA and PPN-evoked EPSCs (n =5, ns; Fig. 1f). These data confirm that all PPN glutamate synapses contain GluR2 subunits, whereas many AMPAR synapses activated by intra-VTA stimulation lack GluR2, indicating that the subunit composition of AMPARs differs in a pathway-specific manner in VTA DA neurons. This would likely result in differences in synaptic strength between these pathways under baseline conditions.

Pathway-specific LTD in VTA DA neurons

The distinct subunit composition of AMPARs targeted by PPN and intra-VTA afferents might be expected to respond differently to stimuli known to induce long-term changes in synaptic strength. Therefore, we examined the capacity of these pathways for LTD.

LTD of intra-VTA-activated EPSCs can be initiated in DA neurons by promoting glutamate overflow onto non-synaptic type 1 metabotropic glutamate receptors (mGluR1) using short bursts of 66Hz stimulation, or by application of the mGluR1 agonist (RS)-3,5-dihydroxyphenylglycine (DHPG) (Bellone and Luscher, 2005). We utilized both DHPG and 66Hz stimulation to evaluate LTD in the PPN and intra-VTA activated pathways. Following a single 66Hz stimulus train within the anterior VTA, 11 of 25 (44%) VTA DA neurons demonstrated LTD of EPSCs evoked via this same pathway (Fig. 2a, 2b), whereas the remaining 14 neurons did not show long-term changes (Fig. 2a).

Figure 2
Selective LTD expression at intra-VTA activated glutamate synapses. (a) Mean time course showing the effects of 66Hz stimulation of the anterior VTA on AMPAR EPSCs in all VTA DA neurons. Intra-VTA-evoked LTD was observed in 11 of 25 cells (44%, Responders). ...

Since the probability of observing intra-VTA-evoked 66Hz-LTD under these baseline conditions was relatively low, we attempted to induce LTD via the PPN pathway in only those neurons showing robust LTD following intra-VTA stimulation. In these experiments, ten minutes of baseline was recorded by alternating single stimuli between the intra-VTA and PPN electrodes in the same neurons. A single 66Hz stimulus train was then applied through the intra-VTA stimulator, followed by single pulse alternating stimulation of the pathways. If LTD of the intra-VTA evoked EPSC was observed 10 minutes following the 66Hz train, then 66Hz stimulation was applied to the PPN, followed again by alternating single pulse stimulation. In these neurons (n = 5), intra-VTA 66Hz stimulation caused robust LTD (Fig. 2c), and this intra-VTA stimulation did not affect EPSCs evoked by single-pulse stimulation of the PPN (Fig. 2c), further demonstrating the independence of these two pathways (Good and Lupica, 2009). However, in contrast to the robust LTD observed in the intra-VTA-activated pathway, 66Hz stimulation of the PPN did not result in LTD, or otherwise affect PPN-evoked EPSCs in any of these same neurons (Fig. 2c). Furthermore, in these same cells, multiple bouts of 66Hz stimulation delivered to the PPN failed to initiate LTD of the PPN-evoked EPSCs in those cells demonstrating robust LTD of the VTA pathway (Fig. 2c).

These data suggest that, in contrast to AMPAR synapses activated by intra-VTA stimulation, the EPSCs evoked via PPN stimulation were incapable of undergoing LTD in the same neurons. However, the design of these experiments necessitated a 20 minute delay in the application of 66Hz stimulation to the PPN to permit assessment of LTD in the VTA pathway. Since this delay may have been sufficient to washout intracellular molecules necessary to initiate LTD, additional recordings were made in another group of VTA neurons (n = 9) in which 66Hz stimulation was applied only through the PPN stimulator approximately 10 minutes after attaining whole-cell access (i.e. the same time as that used to initiate LTD following intra-VTA stimulation). LTD was not observed in any of these neurons following 66Hz stimulation of the PPN (Fig. 2d). Therefore, under control conditions, LTD was observed only after 66Hz stimulation of the intra-VTA pathway, and not following the activation of glutamate afferents originating in the PPN.

The mGluR1 agonist DHPG can also induce LTD of EPSCs in VTA DA neurons activated via intra-VTA stimulation (Bellone and Luscher, 2005). Similar to our result with 66Hz stimulation described above, EPSCs activated via intra-VTA stimulation demonstrated LTD in 20/25 neurons (80%) following 5 min exposure of the slices to DHPG (50 μM), whereas none (0/11, 0%) of the PPN-evoked EPSCs were significantly altered by this drug (Fig. 2e,f).

Since mGluR-induced LTD is thought to occur when higher conductance, calcium permeable, GluR2-lacking AMPARs are removed from activated synapses and replaced with lower conductance GluR2-containing AMPA receptors (Mameli et al., 2007), we sought to determine the presence or absence of GluR2 at these AMPAR synapses using JTx after DHPG-LTD was established in the intra-VTA-activated pathway. Despite the inhibition of intra-VTA-evoked EPSCs by JTx in the majority of neurons under baseline conditions (Fig. 1b), this polyamine did not affect EPSCs evoked via this pathway following DHPG-induced LTD (n = 4; Fig. 2e,f). Furthermore, as expected from experiments shown in Fig. 1, JTx caused no change in the PPN-evoked EPSCs following DHPG exposure. This suggests that mGluR-LTD in the intra-VTA pathway was associated with an increase in synaptic GluR2-containing AMPARs.

These data demonstrate that under basal conditions AMPARs targeted by PPN glutamate afferents contain the GluR2 subunit, and do not exhibit 66Hz or DHPG-induced LTD, whereas some AMPARs activated by intra-VTA stimulation appear to lack GluR2 and exhibit LTD. Thus the subcortical glutamatergic inputs to VTA DA neurons exhibit distinct functional properties compared to those activated via intra-VTA stimulation in the same cells.

A single exposure to cocaine changes AMPAR subunit composition

Exposure to cocaine in vivo can initiate the exchange of GluR2-containing AMPARs for higher conducting GluR2-lacking AMPARs at intra-VTA-activated synapses (Bellone and Luscher, 2006; Mameli et al., 2007), and this results in potentiation of these synapses (Argilli et al., 2008). This effect of cocaine can then be reversed through reinsertion of the lower conducting GluR2-containing AMPA receptors at these synapses following activation of mGluR1s, via either 66Hz stimulation or application of DHPG (Bellone and Luscher, 2006; Mameli et al., 2007). Since we found that JTx had no effect on the PPN glutamate synapse onto VTA DA neurons (Fig. 1), and LTD was not observed in this pathway (Fig. 2), we hypothesized that AMPARs containing GluR2 mediated these EPSCs under basal conditions, and that cocaine exposure might cause a switch from GluR2-containing to GluR2-lacking AMPARs at these PPN-activated synapses.

To investigate this, we measured the effects of NASPM (a synthetic analog of JTx) on both PPN and intra-VTA evoked responses in VTA DA neurons obtained from rats receiving a single i.p. injection of cocaine (15 mg/kg) 24 hours prior to brain slice preparation. In contrast to JTx or NASPM, which had no effect on PPN-evoked EPSCs in slices from drug-naïve animals (Fig. 1), NASPM reduced EPSCs that were evoked via both intra-VTA (n =7) or PPN stimulation (n = 6) in 100% of the cells obtained from cocaine-injected animals (Fig. 3a). This suggests that a single exposure to cocaine initiated the insertion of GluR2-lacking AMPARs into both synaptic pathways.

Figure 3
A single cocaine injection changes AMPAR subunit composition, leading to reliable LTD at both intra-VTA and PPN-activated synapses in the same VTA DA neurons. (a) Mean time course of the effect of the JTx analog NASPM (horizontal bar) on EPSCs evoked ...

To determine whether this presumed change in AMPAR subunit composition following cocaine administration might also permit the more robust expression of LTD in these pathways we applied 66Hz stimulation within the VTA or the PPN, or examined the response of these pathways to DHPG after a single injection of cocaine. Twenty four hours after the cocaine injection, DHPG-induced LTD was observed in both the intra-VTA and PPN-activated glutamatergic pathways in all of the VTA DA neurons (6/6, Fig. 3b,c). We next examined the effect of 66Hz stimulation on the intra-VTA pathway, followed 10 minutes later by 66Hz stimulation applied to the PPN pathway (8 neurons; Fig. 3d). In contrast to our initial experiments where 66Hz-LTD was never seen in the PPN-activated pathway (Fig. 2a, 2c, 2d), LTD was now observed in both pathways in brain slices from animals receiving single cocaine injections, and this was accompanied by significant changes in the RI (intra-VTA stimulation, control RI = 3.90 +/− 0.37, after LTD RI = 2.41 +/− 0.19, n = 8, P < 0.005, paired t-test; PPN pathway, control RI = 3.05 +/− 0.24, after LTD RI = 2.04 +/− 0.19, n = 8, P < 0.01, paired t-test; Fig. 3f). This suggests that GluR-2 lacking AMPARs were inserted at intra-VTA- and PPN-activated synapses following a single exposure to cocaine, and that these higher conducting, Ca2+ permeable receptors were subsequently removed from these synapses during LTD, thus leading to a significantly smaller RI.

Collectively, these data demonstrate that a single exposure to cocaine can alter the AMPAR subunit composition of both subcortical PPN-activated, and locally-activated synapses onto VTA DA neurons, resulting in more efficacious excitatory input that is then subject to modification by LTD.

Δ9-THC induces a pathway- specific change in AMPA subunit composition

Although prior studies have shown that GluR2-lacking AMPARs are inserted into intra-VTA-activated synapses on DA neurons after exposure to cocaine (Bellone and Luscher, 2006), no studies have determined whether exposure to the psychoactive constituent of marijuana, Δ9-THC, affects these synapses, nor have comparisons among glutamatergic pathways been made. Therefore, rats were given single injections of Δ9-THC (i.p., 10 mg/kg) 24 hours prior to preparation of brain slices, and the properties of intra-VTA and PPN-activated glutamatergic synapses were assessed in the same VTA DA neurons.

Unlike the data obtained following a single injection of cocaine, in which PPN and intra-VTA activated glutamatergic synapses were similarly affected (Fig. 3), Δ9-THC exposure selectively altered the subcortical PPN afferent pathway. Specifically, following Δ9-THC exposure, DHPG-LTD was observed in only a portion (7/12 cells, 58%) of the intra-VTA-activated synapses (Fig. 4a,c), but was seen in the PPN-activated pathway in every VTA DA neuron studied (13/13, 100%; Fig. 4a,c). Since the proportion of intra-VTA-activated synapses demonstrating DHPG-induced LTD was not significantly different than that observed in control rats that were not injected with Δ9-THC (no Δ9-THC = 20/25 vs. Δ9-THC injected = 7/12; Z score = 0.99, ns, p = 0.16), we conclude that the drug selectively enabled LTD of PPN glutamate synapses, without altering the expression of LTD in glutamate synapses activated via intra-VTA stimulation.

Figure 4
Afferent-specific LTD at PPN-glutamate synapses following a single exposure to Δ9-THC. (a) Mean time course showing that DHPG initiates LTD of PPN-evoked EPSCs (n = 16/16), but not of intra-VTA-evoked EPSCs, in the same subpopulation of VTA DA ...

To determine whether this pathway-specific expression of LTD was also present with high frequency stimulation, we first applied the 66Hz stimulus the intra-VTA pathway, followed 10 min later by the PPN pathway. Consistent with the results observed with DHPG, 66Hz stimulation applied to the intra-VTA pathway initiated LTD in only a portion of the cells (11/20 cells, 55%), whereas the EPSCs elicited via PPN stimulation demonstrated LTD in all neurons (16/16; Fig. 4b,d). Also similar to the DHPG application was the observation that the proportion of cells demonstrating 66Hz-LTD, evoked via intra-VTA stimulation after Δ9-THC exposure (11/20), did not significantly differ from that observed in rats that had not been given Δ9-THC (16/30; Z = −0.174, p = 0.57), further suggesting that the PPN glutamatergic synapses on VTA DA neurons were selectively altered by Δ9-THC.

To determine whether the Δ9-THC-induced increase in LTD susceptibility of the PPN glutamate pathway was mediated by the incorporation of GluR2-lacking AMPARs into these synapses, we measured the RI. In support of this mechanism, the RI of PPN-evoked EPSCs was significantly larger after Δ9-THC treatment than in control neurons (control RI = 3.1 +/− 0.56, n = 3, after Δ9-THC RI = 4.22 ± 0.55; n = 7, P < 0.05, t-test), and this value was significantly reduced in neurons from Δ9-THC-treated rats following 66Hz-LTD (post LTD RI = 2.62 ± 0.40; P < 0.01, paired t-test, Fig. 5c). Additionally, as expected, the baseline RI (n = 10, 3.97 ± 0.18) in the neurons demonstrating intra-VTA pathway LTD (VTA Responders) was significantly larger (P < 0.0003, paired t-test) than that measured after 66Hz-LTD induction (n = 9, 2.86 ± 0.16; Fig. 5c), whereas in the cells that did not demonstrate LTD (VTA Non-Responders) this value was not significantly affected (baseline RI = 3.45 +/− 0.25; post Δ9-THC RI = 3.41 ± 0.38, n = 7, p > 0.05, paired t-test Fig. 5a).

Figure 5
A single injection of Δ9-THC leads to a pathway specific change in the subunit composition of synaptic AMPAR. (a) The RI of intra-VTA-activated EPSCS is not altered by 66Hz stimulation following Δ9-THC administration, in a subset of VTA ...

To determine whether the change in AMPAR subunit composition caused by Δ9-THC was mediated by cannabinoid receptors, another group of rats was injected with the cannabinoid CB1 receptor (CB1R) antagonist AM251 [N-(piperidin-1-yl)-5-(4-iodophonyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide] (i.p., 2mg/kg), 30 minutes prior to the single Δ9-THC injection. LTD was not observed in any of the VTA DA neurons from animals given both AM251 and Δ9-THC injections following 66Hz stimulation of the PPN pathway (n = 5, Fig. 4e), confirming that the AMPAR subunit change initiated by Δ9-THC occurred as a result of CB1R activation by this drug. Furthermore, 66Hz-LTD was observed in a proportion of the VTA DA neurons using intra-VTA stimulation 24 hours after the AM251 and Δ9-THC injections (3/9, 33%), and this proportion was not significantly different than that observed from control animals (16/30, 53%; Z = 0.67, ns, p = 0.25). Additionally, AM251 alone had no effect on AMPAR subunit composition. Thus, in neurons from rats given a single injection with this drug, LTD was observed in only a portion of the intra-VTA activated synapses (6/14, 43%), which did not differ from that observed in neurons from rats not receiving AM251 (16/30, 53%; Z = 0.32, p = 0.37), and was never observed in the PPN-activated glutamate synapses in the same cells (0/9 cells; Fig. 4f, Fig. 6).

Figure 6
Compilation of the effects of all manipulations on EPSC amplitudes for all neurons included in this study. The numbers in parentheses represent the total number of VTA DA neurons in which observations were made during each manipulation. Gray circles indicate ...

These data demonstrate that CB1Rs were activated by Δ9-THC, and this selectively caused the insertion of higher conducting GluR2-lacking AMPARs into PPN glutamatergic synapses. This change in AMPAR subunit composition was then permissive to LTD expression only in the PPN pathway.

Discussion

The present study demonstrates that single VTA DA neurons are innervated by distinct glutamatergic afferents that activate either AMPARs containing the GluR2 subunit or AMPARs lacking this subunit. Thus, under baseline conditions, subcortical PPN glutamatergic afferents formed synapses on Ca2+-impermeable GluR2-containing AMPARs, whereas the afferents activated by conventional intra-VTA stimuli targeted Ca2+-permeable AMPARs that predominantly lacked this subunit. This demonstrates that pathway-specific differences in AMPAR subunit composition exist, and because prior results demonstrate that AMPARs lacking GluR2 subunits exhibit higher single channel conductances and greater permeability to Ca2+ (Swanson et al., 1997; Isaac et al., 2007), our data indicate that PPN inputs are likely weaker than those of other glutamate afferents to VTA DA neurons.

Pathway-specific AMPAR subunit composition results in differential expression of LTD in VTA DA neurons

Previous studies demonstrated that mGluR-dependent LTD at intra-VTA-activated synapses on DA neurons is distinct from that observed in other brain regions, such as cerebellum and hippocampus (for review, Luscher and Huber, 2010). In these regions LTD results from the endocytosis of AMPARs (Argilli et al., 2008; Luscher and Huber, 2010), whereas in VTA DA neurons LTD requires the exchange of GluR2-lacking AMPARs for those containing this subunit, following mGluR1 activation (Luscher and Huber, 2010). Our study confirmed prior observations by showing that mGluR-LTD at the intra-VTA-evoked pathway was only observed when the AMPARs underlying these responses lacked the GluR2 subunit. Our data also extend these findings by demonstrating that the baseline capacity for LTD was input-specific, since glutamate synapses activated by subcortical PPN stimulation never exhibited LTD in the same neurons in which intra-VTA-activated LTD was observed. Based upon the prior observation that mGluR-dependent LTD in VTA DA neurons required synthesis and insertion of lower-conductance GluR2-containing AMPARs (Mameli et al., 2007), we hypothesize that our observed resistance of the PPN-activated pathway to LTD resulted from the presence of GluR2-containing AMPARs at these synapses under basal conditions. This was confirmed by the absence of polyamine sensitivity and lack of EPSC rectification. The presence of Ca2+-impermeable, lower-conductance, GluR2-containing AMPARs at PPN-activated synapses suggests that these afferents will have smaller effects on VTA DA neuron activity under basal conditions than other glutamate synapses lacking this subunit.

A single exposure to cocaine decreases GluR2-containing AMPARs at PPN- and intra-VTA-activated glutamate synapses on the same VTA DA neurons

Under baseline conditions PPN-activated afferents targeted polyamine insensitive GluR2-containing AMPARs on VTA DA neurons. However, 24 hours following a single cocaine injection, EPSCs evoked via this pathway were inhibited by NASPM, demonstrating a loss of GluR2-containing AMPARs. This was correlated with changes in rectification of PPN-activated EPSCs. Based on other studies using only intra-VTA stimulation, this switch from polyamine-insensitivity to full sensitivity in the PPN pathway likely resulted from a cocaine-induced exchange of GluR2-containing AMPARs for those lacking this subunit (Bellone and Luscher, 2005; Mameli et al., 2007). The insertion of GluR2-lacking AMPARs at intra-VTA activated synapses following cocaine treatment caused a marked increase in the incidence of both 66Hz-, and DHPG-induced LTD, such that all cells demonstrated this form of plasticity. However, the appearance of LTD at PPN activated synapses was particularly notable because it was completely absent using 66Hz or DHPG induction paradigms in control DA neurons, but was seen in all neurons 24 hours after the single in vivo cocaine injection.

The observation that independent glutamatergic afferents underwent similar changes following cocaine exposure suggests that this drug broadly triggers the insertion of GluR2-lacking AMPARs at all glutamate synapses on VTA DA neurons, thereby priming these synapses for subsequent long-lived changes in synaptic strength upon activation by the appropriate afferent signal. This switch in AMPAR subunit composition resulting in increased EPSC amplitude has been likened to LTP, since cocaine exposure increases glutamatergic transmission, and occludes spike-timing-dependent LTP (Argilli et al., 2008). Consistent with this, we showed that cocaine potentiated glutamate synapses activated by intra-VTA and PPN stimulation. Since under basal conditions PPN inputs to VTA DA neurons targeted only low-conductance GluR2-containing AMPARs, the cocaine-induced potentiation of these PPN synapses would have a proportionally greater effect on DA neuron activity than intra-VTA-activated inputs. This is because there is a larger proportional change in the AMPARs underlying excitatory transmission in the PPN projection, and the strength of the PPN influence on VTA DA neurons is predicted to shift abruptly from weak to strong following cocaine exposure, whereas the intra-VTA activated pathway would exhibit a smaller relative change.

Exposure to Δ9-THC selectively alters subcortical PPN glutamate inputs to VTA DA neurons

Although the effects of several abused drugs on VTA DA neuron AMPARs have been examined (Faleiro et al., 2004; Bellone and Luscher, 2006; Lane et al., 2008), the effects of Δ9-THC on AMPAR subunit composition has not been studied. As with cocaine, a single injection of Δ9-THC altered AMPAR subunit composition in VTA DA neurons. However, unlike the similar effects of cocaine on PPN and intra-VTA-activated synapses, Δ9-THC caused a switch to GluR2-lacking AMPARs in only the PPN-activated pathway, and this was associated with the appearance of DHPG-LTD. Thus, although PPN-activated EPSCs never demonstrated LTD in control neurons, it was observed in all PPN-activated DA neurons following Δ9-THC. LTD was also specific to PPN-activated synapses, since the proportion of intra-VTA-activated EPSCs demonstrating LTD did not change following Δ9-THC injection.

The permissive effect of Δ9-THC on PPN pathway LTD was mediated by CB1Rs since the antagonist AM251 prevented both LTD and changes in EPSC rectification. Although CB1Rs were implicated in the Δ9-THC effect, we did not determine whether these CB1Rs were located in the VTA, or in brain circuits that engage this nucleus. Previously, the potentiating effect of cocaine on intra-VTA-activated synapses was seen in as little as 3 hours after cocaine exposure, either via a single injection, or through direct in vitro application (Argilli et al., 2008). Thus, cocaine could affect AMPARs through direct actions within the VTA, independently of interaction with circuits impinging upon the DA neurons (Argilli et al., 2008). This direct cocaine effect may explain the lack of selectivity with regard to PPN versus intra-VTA activated afferents in the present study, and further suggests that the more selective effect of Δ9-THC on the PPN projection may result either from altered output of this nucleus, or perhaps to a more direct action within the VTA that involves only PPN synapses. Previously, our laboratory and others have shown that CB1R agonists inhibit glutamatergic neurotransmission (Melis et al., 2004; Riegel and Lupica, 2004). However, the origin of the intra-VTA-activated glutamate afferents were not identified. In addition, CB1R activation inhibits GABA inputs to GABAB (Riegel and Lupica, 2004), and GABAA receptors on VTA DA neurons (Szabo et al., 2002), and anatomical evidence has confirmed the presence of CB1Rs in the VTA (Matyas et al., 2008). Therefore, although there is evidence that cocaine can alter AMPAR subunit composition via direct interaction in the VTA, it is unclear whether Δ9-THC effects on subcortically-activated AMPARs occur locally, or via effects on VTA projecting nuclei. The present results suggest that a determination of CB1R actions on the PPN pathway is also warranted.

Functional consequences of drug-induced AMPAR subunit changes

An increase in the involvement of GluR2-lacking AMPARs at synapses on VTA DA neurons following exposure to abused drugs is expected to have important functional consequences for this central component of the brain reward circuitry. Thus, following single exposure to cocaine (Argilli et al., 2008) or Δ9-THC (present study), a switch to GluR2-lacking AMPARs will strengthen glutamatergic synaptic transmission. Furthermore, since GluR2-lacking receptors gate Ca2+, they may provide an additional mechanism through which intracellular Ca2+ levels can be increased to affect biochemical signaling processes (Carlezon and Nestler, 2002; Isaac et al., 2007). Our experiments, and others, demonstrate that brief exposure to abused drugs can alter AMPARs, which can then serve as a substrate for additional changes in synaptic efficacy through processes such as LTD. This suggests that altered AMPAR subunit composition represents a dynamic mechanism to mediate synaptic changes resulting from experience, including those occurring through passive exposure to, or self administration of abused drugs, and that this can lead to rapid adaptation of neuronal circuits (Carlezon and Nestler, 2002; Bellone and Luscher, 2006; Kauer and Malenka, 2007; Mameli et al., 2007; Conrad et al., 2008). Since AMPAR subunit rearrangements in the VTA occur before those in other reward-relevant brain nuclei, it is suggested that this may represent an early adaptation prior to recruitment of downstream targets, and the behavioral progression from casual drug use to addiction (Carlezon and Nestler, 2002; Mameli et al., 2007). We show that the involvement of GluR2-lacking AMPARs can be selectively increased at subcortical PPN glutamate synapses following exposure to Δ9-THC, and that this can provide a substrate to permit long-term changes in synaptic plasticity at these inputs. This, and our observation that cocaine can more broadly induce such changes at intra-VTA- and PPN-activated glutamate synapses, suggests that some abused drugs may recruit distinct glutamatergic circuits, and thereby selectively alter the degree of influence that certain brain nuclei have over VTA DA neuron function. Based on the pattern of AMPAR subunit changes following Δ9-THC exposure, we predict that unlike psychostimulants, subcortical glutamate inputs to VTA DA neurons will be more strongly and selectively affected by Δ9-THC, and we suggest that this may represent a neural code that permits the reward signal provided by Δ9-THC to be distinguished from other abused drugs.

Acknowledgments

This work was supported by The U. S. Department of Health and Human Services, the National Institutes of Health, and the National Institute on Drug Abuse Intramural Research Program. We would like to thank Drs. Cristina Bäckman and YaJun Zhang for performing DAT immunohistochemistry and biocytin reconstructions of VTA neurons, and Drs. Alex Hoffman, Eisuke Koya, and Bruce Hope for helpful comments on the manuscript.

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