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Logo of jcinvestThe Journal of Clinical InvestigationCurrent IssueArchiveSubscriptionAbout the Journal
J Clin Invest. 2010 July 1; 120(7): 2249.
Published online 2010 July 1. doi:  10.1172/JCI43731
PMCID: PMC2898618

In This Issue

Cancer in a PINCH(1)

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One of the main obstacles to complete elimination of a cancer is tumor cell resistance to ionizing radiation therapy and chemotherapy. One factor contributing to this is tumor cell adhesion to extracellular matrix mediated by macromolecular complexes known as focal adhesions. In this issue (2516–2527), Eke and colleagues find that particularly interesting new cysteine-histidine-rich 1 (PINCH1) functions downstream of focal adhesions to promote tumor cell radioresistance. Initial analysis indicated that PINCH1 expression was elevated in many different human tumors. The potential clinical importance of this was highlighted by the observation that mouse cells lacking PINCH1 showed enhanced sensitivity to ionizing radiation and a chemotherapeutic in vitro and to ionizing radiation in vivo. Further in vitro analysis uncovered the molecular mechanism of the prosurvival effect of PINCH1: it directly binds to protein phosphatase 1α (PP1α), which is an Akt1-regulating protein, and inhibits PP1α activity, resulting in increased Akt1 phosphorylation and enhanced resistance to ionizing radiation. The authors therefore suggest that targeting molecules such as PINCH1 that function downstream of focal adhesions might provide new therapeutic approaches to overcoming tumor cell resistance to ionizing radiation therapy and chemotherapy.

The p38 MAPK axis of bone homeostasis

MAPK signaling pathways are triggered by many of the extracellular ligands that regulate bone homeostasis. Despite this, the relative contribution of specific MAPKs has not been clearly determined. To study the role of the p38 MAPK pathway in osteoblast biology, Greenblatt and colleagues analyzed mice in which different components of the pathway were genetically disrupted (2457–2473). Initial analysis indicated that mice with osteoblast-specific deletion of TGF-β–activated kinase 1 (Tak1) exhibited bone defects similar to those observed in humans haploinsufficient for runt-related transcription factor 2 (Runx2), the master regulator of osteoblast development and bone formation. Further analysis indicated that TAK1 was the crucial upstream activator of p38 MAPKs, working via MAPK kinase 3 (MKK3) and MKK6. In turn, the MKK3/6-activated p38 MAPKs phosphorylated Runx2, promoting its regulation of osteoblast genetic programs. Among the four p38 MAPK isoforms, p38α and p38β were both found to contribute to in vivo bone formation. Collectively, these data indicate that the p38 MAPK pathway is required for normal skeletogenesis in mice and suggest that selective p38β agonists may prevent the bone loss associated with osteoporosis and aging.

Reducing the toxicity of lithium

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Lithium is the most effective treatment for bipolar disorder. However, its use is limited because of neurological side effects and a risk of overdose-induced toxicity. Many of the beneficial effects of lithium are thought to be mediated by its inhibition of glycogen synthase kinase-3 (GSK-3), but whether this is the mechanism underlying its negative effects has not been determined. To investigate this, Raquel Gómez-Sintes and José J. Lucas studied the effects of chronic administration of therapeutic doses of lithium to wild-type mice (2432–2445). This treatment regimen resulted in decreased GSK-3 activity in brain, gait abnormalities, and neuronal apoptosis in several regions of the brain. Further analysis showed increased nuclear localization of nuclear factor of activated T cells c3/4 (NFATc3/4) transcription factors and increased levels of Fas ligand in regions of the brain affected by neuronal apoptosis. Consistent with this, lithium-induced neuronal apoptosis and gait abnormalities were absent when nuclear translocation of NFAT was prevented by cyclosporin A administration and in Fas-deficient mice. The authors hope that their delineation of a molecular pathway by which lithium induces neuronal apoptosis and gait abnormalities in mice might provide new ideas for combination therapies that diminish the toxicities of lithium.

Periostin promotes heart valve degeneration

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Valvular heart disease (VHD) is any disease process involving one or more of the heart valves. There are many causes of VHD, including atherosclerosis and rheumatic fever, but no therapies to prevent progressive valvular degeneration. Greater insight into the mechanisms underlying VHD pathogenesis is therefore needed. To address this issue, Hakuno and colleagues analyzed degenerated human cardiac valves from patients with either atherosclerotic or rheumatic VHD and found aberrant expression of periostin (2292–2306), a protein that plays critical roles in cardiac valve development. Specifically, periostin levels were markedly increased in infiltrated inflammatory cells and myofibroblasts in areas of angiogenesis. Consistent with these data having pathophysiologic relevance, the increased aortic valve defects observed in wild-type mice fed a high-fat diet were attenuated in mice lacking periostin. Further in vitro analysis indicated that periostin acts on endothelial cells to promote angiogenesis and on several cell types in the heart to promote secretion of MMPs, enzymes implicated in the progression of various degenerative diseases. These data provide new insight into the molecular mechanisms underlying VHD and suggest that periostin may be a therapeutic target to prevent progressive valvular degeneration in atherosclerotic and rheumatic VHD.

Articles from The Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation