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Clin Vaccine Immunol. 2010 July; 17(7): 1055–1065.
Published online 2010 May 12. doi:  10.1128/CVI.00131-10
PMCID: PMC2897268

Correlates of Protection Induced by Vaccination[down-pointing small open triangle]


This paper attempts to summarize current knowledge about immune responses to vaccines that correlate with protection. Although the immune system is redundant, almost all current vaccines work through antibodies in serum or on mucosa that block infection or bacteremia/viremia and thus provide a correlate of protection. The functional characteristics of antibodies, as well as quantity, are important. Antibody may be highly correlated with protection or synergistic with other functions. Immune memory is a critical correlate: effector memory for short-incubation diseases and central memory for long-incubation diseases. Cellular immunity acts to kill or suppress intracellular pathogens and may also synergize with antibody. For some vaccines, we have no true correlates, but only useful surrogates, for an unknown protective response.

The correlates of vaccine-induced immunity are a subject of continued interest for both theoretical and practical reasons. The latter include the need to evaluate the consistency of vaccine production; the susceptibilities of individuals and populations after vaccination; the validation of vaccines for which efficacy trials are not ethical, as when a prior-generation vaccine is already licensed; and the licensure of combination vaccines (38). I have twice previously reviewed knowledge in this area, first in a general overview (138) and second to define the notions of correlates and surrogates of protection (139). This article attempts to survey all examples known to me of immune responses to licensed vaccines that correlate with protection and is an update of the overview published in 2001, including excerpts used by permission of the original journal, Pediatric Infectious Diseases. The reader is also referred to one of the preceding articles for conceptual discussion (139).

For clarity, Table Table11 gives definitions of correlates and surrogates that are used in this article. Although the distinctions may be semantic, the literature is confusing, because some authors use the words correlate and surrogate interchangeably, whereas precision in language is preferable. In essence, I define an immune function that is responsible for protection as a correlate, while an immune response that is simply an easy measurement but not functional in protection is a surrogate.

Definitions of terms used in this article


There are at least six general principles that should be understood before taking up specific examples. They are as follows. (i) Large challenge doses may overwhelm vaccine-induced immunity and confuse the identification of correlates (117, 142). However, the relationship between the dose and failure of protection may not be linear, as demonstrated recently for hepatitis B infection of chimpanzees (7). (ii) The mechanism of protection is not necessarily the mechanism of recovery from infection. The latter are often cellular immune functions, which may be irrelevant to vaccine-induced prevention of infection (124, 133, 134). (iii) Indeed, most vaccines available today act through antibodies, on condition that they are functional (137), as is evident from the frequent efficacy of passive antibodies and transplacental antibodies (203). However, as will be repeatedly emphasized in this article, the immune system has evolved to be redundant, and vaccines, like prior natural infection, may protect through multiple mechanisms. (iv) Memory induced by vaccination may be crucial to protection, particularly in long-incubation diseases, such as hepatitis B (86, 185). Although loss of antibody after vaccination may render vaccinees again susceptible to some infections (90), central memory established by vaccination is sufficient under certain circumstances to confer protection (20). (v) Correlates may vary according to individual characteristics, such as age, gender, and major histocompatibility complex (MHC) group (see the discussion of influenza below). (vi) It is important to define protection against what. Correlates may differ quantitatively and qualitatively, depending on whether the objective is to prevent systemic infection, mucosal infection, disease, or severe disease.


Where protection is based on anticapsular antibody or antitoxin antibody, it has been relatively easy to define correlates. For bacteria that do not have capsules to prevent phagocytosis or toxins that are pathogenic, the situation becomes more complex.

For the three main bacterial pathogens that cause bacteremic disease—Haemophilus influenzae type b (Hib), pneumococci, and meningococci—the correlates are opsonophagocytic or bactericidal antibodies, although binding antibodies are useful as surrogates. In the case of H. influenzae, binding antibodies are routinely used to define protection, as indicated in Table Table2.2. A correlate of immunity is best known for the Hib polysaccharide, which was formerly used as a vaccine (74). In Finland, before the use of the vaccine, the age-specific incidence of Hib disease declined significantly at the age when most of the population showed antibody concentrations of 0.15 μg/ml or more, and in the efficacy study of the polysaccharide (94), age-specific vaccine efficacy was correlated with the induction of 1.0 μg/ml of antibody postvaccination. Consequently, an antibody level of 0.15 μg/ml is probably protective against bacteremia and is likely to remain present for some period in vaccinees who respond initially with 1 μg/ml of antibody.

Quantitative correlates and surrogates of protection after vaccination

Even more direct information is available from studies of gamma globulin prophylaxis and of the decline of maternal antibodies in relation to disease incidence (94, 151, 159). These studies support the idea that concentrations between 0.03 and 0.1 μg/ml are protective, and thus, the commonly accepted 0.15-μg/ml cutoff seems reasonable (73). However, more recently, three important points have emerged with regard to protection against Hib: (i) that anamnestic responses are insufficient and that the antibody must be present at the time of exposure (6, 90, 147, 184), (ii) that the antibodies must be of high avidity in order to protect (82), and (iii) that with the T-cell-dependent response reduced by protein conjugates, a 1ower concentration may be protective (73).

Prevention of nasopharyngeal carriage is achieved through diffusion of H. influenzae antibodies from serum and is correlated with postimmunization levels of >5 μg/ml (44).

Pneumococcal antibodies are also often measured by enzyme-linked immunosorbent assay (ELISA), but in the very young and in elderly adults, these antibodies tend not to be opsonophagocytic, which accounts for the relatively poor efficacy of unconjugated polysaccharides that elicit only binding antibodies in the aged (153). The protective level of antibody as measured by ELISA has been variously calculated, but there is a reasonable consensus that it lies between 0.18 and 0.35 μg/ml (68, 81, 127, 167). The critical issue is the relationship between ELISA values and functional opsonophagocytic antibodies, which may vary with serotype (81). Goldblatt et al. found that at 0.2 μg/ml antibody by ELISA, most vaccinees were positive for opsonophagocytic antibodies (54). A bactericidal titer of 1/8 for those antibodies may correlate with protection (42). However, a recent analysis of a study done in Africa, where the efficacy of the vaccine was lower, showed a correlate of 2.3 μg/ml (156). The more compressed vaccine schedule used in Africa or a higher challenge dose of pneumococci may account for this difference. Thus, the protective concentration appears to vary, depending on the population, the serotype, and the clinical end point.

Prevention of nasopharyngeal carriage of pneumococci is important to individual and herd immunity. Diffusion of IgG antibodies from serum is thought to correlate with protection against carriage (31). However, the situation may be more complex, as there is evidence in mice that prevention of pneumococcal carriage correlates with a Th17 cellular response (202). Moreover, antibody response to the PspA surface protein of pneumococci may also correlate with prevention of carriage (101).

Humoral responses to meningococci also may be measured by ELISA, but only bactericidal tests correlate with protection, as is demonstrable in children, who develop the former but not the latter after unconjugated polysaccharide immunization. The level of bactericidal antibody necessary for protection depends on the complement used in the test, but with human serum, a level of >1/8 or even >1/4 is usually considered sufficient for all serogroups, including outer membrane vesicle vaccines against group B (17). In adults, this correlates with an ELISA antibody measurement of 2 μg/ml (96, 132).


Correlates of protection are particularly clear for the class of toxin-producing bacteria (Table (Table2).2). Tetanus and diphtheria have been well studied, and the levels of antitoxin after vaccination that correspond to protection were established years ago. For both pathogens, a level of 0.01 μg/ml provides considerable protection, whereas a level of 0.1 μg/ml corresponds to virtually complete protection against the respective diseases (56, 65, 89, 100, 113), although more antibody may be required for diphtheria (92). Exceptional cases of diphtheria and tetanus occur despite high concentrations of antibodies, perhaps because of poor diffusion into sites of toxin production, but the illnesses are usually mild (13, 14, 34, 37, 65). Measurement of antitoxin in animals is preferable to in vitro methods, as the latter may also detect nonneutralizing antibodies (36).

Bacillus anthracis acts through toxin production, although its capsule is also a virulence factor. For obvious reasons, there are no data on human challenge, but two methods are generally used to measure resistance against an aerosol challenge in animals: an ELISA binding the “protective-antigen” (PA) part of the toxin, and toxin neutralization (TN). It appears that PA antibodies at more than 100 units and TN antibodies at more than 1/1,000 correspond to good protection (87, 136, 149, 170, 191).

The case of pertussis is more complex, as in addition to the pertussis toxin, the vaccines usually contain one or more attachment factors, which also may be protective. Therefore, protection correlates with antibodies not only against pertussis toxin (PT), but also against pertactin and fimbrial hemagglutinins. The exact level of each of these antibodies that is protective is controversial, and in any case, there is no absolute threshold, but 5 to 10 units of ELISA antibodies to PT have been proposed as an important benchmark (25, 173, 174, 180).


The only bacterial infection that is transmitted by insects for which a vaccine has been developed is Lyme borreliosis. Although no longer licensed, the vaccine acted by inducing antibodies against the outer surface protein A of the organism, which neutralized Borrelia burgdorferi in the intestine of the tick vector at the time of the blood meal. Retrospective analysis of the efficacy trial allowed the determination of a protective level of antibodies equivalent to 1,100 enzyme immunoassay (EIA) units/ml after immunization and 400 EIA units/ml at the time of the tick bite (128).


The causative agent of tularemia is partially susceptible to the action of passive antibody. However, induction of cellular immunity in addition to antibody is necessary for maximum protection (161, 165).

On the other hand, protection against the plague bacillus appears to be mediated by antibodies, particularly those directed against the LcrV virulence antigen, but also those directed against the F1 capsular antigen. The ability to neutralize in vitro more than 20% of cytotoxicity by the organism in macrophages correlates with protection (197, 201).

It is generally believed that cellular immunity induced by Mycobacterium bovis BCG is the protective function against tuberculosis, although this is largely, though not exclusively, an extrapolation from animal studies of infection (59, 179). Immunity correlates best with CD4+ cells that produce cytokines, particularly interleukin 23 (IL-23), which stimulates IL-17-producing CD4+ cells, which in turn stimulate gamma interferon at the time of challenge, and it is the presence of the last in the lungs that is protective (46, 75, 106). However, CD8+ cells contribute to protection (119), and it has been argued that antibodies play a role in enhancing uptake of mycobacteria by macrophages (1). No firm conclusion can yet be drawn.


Vaccines against viruses injected into the bloodstream appear to be a straightforward case for antibodies, but as we shall see, the situation is more complex.

To be sure, it was established many years ago that neutralizing antibodies induced by yellow fever vaccine correlate well with protection (97), although early after immunization, innate immune responses play an important role (146, 166). However, in general, a level of 0.7 neutralization units, equivalent to a 1/5 titer, is considered protective (97).

Japanese encephalitis vaccines are also based on induction of antibodies, and a neutralization titer of 1/10 is considered protective (55, 63). Although no licensed vaccine yet exists for West Nile virus, it is interesting that, whereas antibodies are protective in mice, once infection of the brain takes place, CD4+ cells are required for clearance from the central nervous system (168).

The case for tick-borne encephalitis seems reasonably clear: antibodies are protective, and a level of 125 ELISA units is considered sufficient (77).

Dengue vaccines are in advanced clinical trials, and therefore, the definition of a correlate of protection has become important. Whereas neutralizing antibody clearly protects against a homologous serotype (157, 175), controversy has centered on what function gives heterologous serotype protection in the face of the enhanced disease that occurs in the presence of antibody-dependent enhancement (ADE) (57). It appears from studies in a mouse model that heterotypic neutralizing antibodies do give heterotypic protection if they are present in concentrations sufficient to protect but insufficient to cause ADE (8, 78, 186).


The largest category of agents for which we have vaccines is viruses that infect the mucosae and then cause viremia, which includes viruses that initially replicate in the nasopharynx and intestine. The classical example is smallpox, the disease for which the first vaccine was developed. The situation for smallpox is clear: antibody provides the best correlate of immunity to infection after vaccination, but cellular responses, particularly CD8+ cells, influence the severity of disease if infection occurs despite antibodies. The evidence for this comes from animal experiments and also from human data showing persistence of antibodies and protection against death virtually forever after vaccination but susceptibility to mild disease once CD8+-mediated cytotoxic T lymphocytes (CTL) have faded away (5, 58, 125, 126). The protective titer of neutralizing antibody appears to be about 1/20 (93, 160) and may be directed against one or several smallpox proteins with neutralizing epitopes (12).

Interestingly, whereas the above is the case for smallpox acquired naturally, cellular immunity plays a more important role in the prevention of poxvirus growth in the skin after an initial introduction by scarification, illustrating the importance of organ-specific factors (88).

The two vaccines against poliomyelitis, inactivated (IPV) and oral (OPV), work in both parallel and different ways. Each vaccine elicits serum antibodies that prevent viremia, and neutralization at titers of 1/8 (or even 1/4) is protective (143, 193).

Live, attenuated polio vaccines are well known to give local immunity to subsequent superinfection (176), although this immunity may be abrogated if the challenge is sufficiently large. IPV has been reputed not to provide the same immunity, but as previously reviewed (41, 109), poliovirus replication in the nasopharynx is reduced after both IPV and OPV vaccination (95), However, the resistance induced by IPV in the intestines, although substantial, is clearly less than that afforded by live vaccine (95, 117), Intestinal resistance is proportional to the serum antibody titer (95), which suggests that passage of IgG into the lumen of the intestine is partly responsible for the local immunity. On the other hand, Ogra and Karzon (115) found that intestinal resistance after OPV was induced only where the vaccine virus had previously replicated and induced a secretory-antibody response. Interestingly, elderly people who had received OPV many years previously and who still had serum and salivary IgA antibodies were resistant to reinfection with OPV, whereas those who lacked those antibodies could be reinfected (19).

With regard to the four live vaccines commonly given in infancy, measles, mumps, rubella, and varicella, antibodies are certainly relevant to protection, but there are important qualifications to take into account. The role of antibodies in protection against measles is indisputable. Gamma globulin has been effective in preventing infection and disease, and maternal antibodies are well demonstrated to protect. The plaque neutralization test shows good correlation with protection (3, 24, 118). Microneutralization titers of ≥120 mIU/ml give protection against disease, whereas titers of ≥1,000 mIU protect against both infection and disease. Cases of secondary vaccine failure may be related to loss of antibodies (98).

However, measles vaccine also induces cellular responses (10, 52), and the importance of cellular immunity is evident when immunosuppressed subjects are vaccinated, for they develop transient protection (76, 155). On the other hand, suppression of cellular responses does not seem to render subjects again susceptible as long as they maintain antibodies (2, 204). Immunosuppression may lead to severe measles in unvaccinated subjects, but that is related to poor control of established replication.

An interesting study was conducted in rural Senegal in which antibodies were measured early after exposure to measles (158). Both unvaccinated and vaccinated children with plaque neutralization titers of <40 mIU/ml were highly susceptible to clinical measles. Unimmunized children with trace amounts of antibody (40 to 125 mIU/ml) still had a high risk of measles, whereas immunized children with such titers were usually protected. Both immunized and unimmunized children with titers of >125 mIU/ml showed a high degree of protection. One interpretation of the results is that unimmunized children were not protected by trace amounts of maternal antibody whereas immunized children had cellular immunity in addition to the trace amounts of antibody (130, 144, 145, 148). Antibodies against the measles virus hemagglutinin are most important, as shown in experiments with DNA-based vaccines (130), but protective neutralizing antibodies against either hemagglutinin or fusion protein can protect (144). However, if infection does take place, T cells must be functional to close off replication, which is why T-cell-deficient children may suffer complications from vaccination (124, 133, 134). The complexity of T-cell action is illustrated by results showing that whereas CD8+ T cells are mainly responsible for viral clearance in the lungs, only CD4+ T cells are necessary for control of virus in the central nervous system (145, 148).

In the case of mumps vaccine, many attenuated strains have been widely used, including Jeryl Lynn, Leningrad, Urabe, and Rubini. Field studies done after vaccination with the Rubini strain showed low efficacy, although the strain appeared to elicit an antibody response. Closer study revealed that whereas the Jeryl Lynn and Rubini strains both induced neutralizing and indirect fluorescent antibodies in the majority of vaccinees, the latter strain induced low titers of ELISA antibodies (22, 164). This surprising result is unexplained but may suggest that antigens other than surface antigens of the virus are important to protection. In this regard, it is interesting that passive immunization against mumps has not been demonstrated to be effective, which again suggests that other factors are important. Differences in neutralizing specificities between vaccine viruses and circulating viruses have been advanced as a reason for vaccine failure, but this remains controversial (33, 62, 71, 114, 154). Titers of 1/2 by neutralization or 1/8 by hemagglutination inhibition (HAI) have been proposed as protective levels (40, 189), but no certain serologic correlate of protection is accepted. T-cell responses to mumps vaccine have been demonstrated, but their protective effect is unknown. The need to define a correlate of immunity to mumps has become acute owing to the recent outbreaks in previously vaccinated young adults who had apparently lost prior immunity (9).

During the early development of rubella vaccine, several live, attenuated strains were proposed and tested clinically. Eventually, the RA 27/3 strain was widely accepted as the standard vaccine strain. Among the reasons for this choice was the fact that, although hemagglutination-inhibiting antibodies were induced by both the RA 27/3 and HPV-77 vaccine strains and by natural infection, only natural infection and RA 27/3 vaccine induced high levels of neutralizing antibodies, which apparently correlated better with protection (141). Other advantages of RA 27/3 included the elicitation of secretory antibody in the nasopharnyx and resistance of vaccinees to challenge with intranasally administered rubella virus (116). In an intranasal challenge study in subjects who had low or absent levels of serum antibodies, seronegative controls were regularly infected and subjects seropositive after natural infection or after RA 27/3 vaccination were resistant, whereas subjects vaccinated with other strains were sometimes infected (121).

In recent years, radioimmunoassay has become the predominant test for immunity to rubella, and 10 IU/ml has been proposed as the correlate of immunity (99, 169). This corresponds to a neutralization titer of 1/8. Although there is a cellular immune response to the vaccine (4), attempts to define a cellular correlate to immunity after rubella vaccination have thus far failed (182).

Clinicians are well aware of the crucial role of intact cellular immune responses in recovery from primary infection by varicella-zoster virus (VZV) (43). In addition, the recurrent disease known as zoster occurs because T-cell immunity flags with age, whereas antibodies persist (60).

Nevertheless, antibodies also play a key role in protection against varicella. Passively administered varicella-zoster immunoglobulin can prevent infection, and there is good correlation between the strength of the antibody response after varicella vaccine and the protection seen after later exposure (27, 69, 195). Titers of >1/64 by fluorescent antibody to membrane antigen (FAMA) give the best indication of protection against disease (79, 80). Healthy vaccinees who do not mount a titer of at least 1/8 do not seem to be protected against infection (107). A VZV glycoprotein (gp) antibody ELISA has also been used as a correlate of protection, and evidence of seroconversion by that assay is related to long-term protection (85), although the gp ELISA can produce false-positive reactions in varicella-susceptible children (107). In one study, CD4+ cell responses were found to be more persistent than antibodies (91).

In contrast, children often resist infection after vaccine-induced antibodies are undetectable, suggesting a protective function for T cells, and agammaglobulinemic children nevertheless become immune after natural infection (53). Thus, antibody measurements correlate with protection against varicella, but it is possible that they are mainly a surrogate for cellular immunity.


Gamma globulin from naturally infected subjects has been used for many years to prevent hepatitis A (172). This circumstance permits one to compute a protective correlate from the concentration of antibodies induced by the dose of IgG shown to be protective in clinical studies. That concentration is ~10 mIU/ml if maintained over a 2-month period, although some individuals may be protected at much lower concentrations (29). The inactivated hepatitis A virus vaccines generate an average concentration 1,000-fold higher than the protective concentrations and also induce immunologic memory (29, 45, 110, 163, 172, 192).

Antibody tests often become negative years after hepatitis B vaccination, yet protection appears to be solid and long-lasting, and no booster vaccination is recommended (30, 185, 188, 193). Postvaccination titers of 10 mIU/ml or greater correlate with the induction of the T helper cell responses that mediate the memory of B cells. Thus, when the vaccinee is exposed to hepatitis B virus, there is an anamnestic response that prevents disease, and often infection as well (23, 30, 48, 64, 108, 178, 185, 187). In a study of children vaccinated 5 to 7 years previously, 46% had antibody titers below 10 mIU/ml. Nevertheless when given a booster dose, 90% showed evidence of an anamnestic response (86). The long incubation period of the disease allows the anamnestic response to be highly protective.


The literature on correlates of protection against influenza is rich, and there is a consensus that in the case of inactivated vaccine, a serum HAI antibody level of about 1/40 against the hemagglutininogen (HA) is protective. However, that level is protective only at 50 to 70%, which makes it a relative rather than an absolute correlate of protection. Evidence that other influenza virus antigens add to the protection afforded by the HA is scant (112). However, one must distinguish between the young and the old with regard to influenza vaccine. In adults up to about age 50 years, serum IgG antibodies correlate well with protection (32, 39, 183). However, inasmuch as influenza virus infection is not normally invasive, the question arises as to how serum antibodies exert an effect. Diffusion of IgG antibodies into the nasopharynx and lungs, as well as some degree of local IgA generation, offer possible explanations (28).

Nevertheless, the importance of cellular immune responses in protection by influenza vaccine has long been moot, based primarily on studies in mice (181). More recently, compelling evidence in humans has been adduced that in the elderly, CTL responses associated with granzyme B production correlate better with protection than does antibody (55a, 102, 103, 150).

The picture with regard to live, attenuated influenza vaccine is clearer. The intranasal vaccine elicits both serum IgG and secretory IgA antibodies, and each class is protective, with synergy when both are present (11). However, cellular immunity induced by replicating vaccine virus may also contribute to protection against clinical symptoms (47).

Arguably, rotavirus vaccination provides the most complex and controversial puzzle with respect to definition of correlates of immunity in current vaccinology. Neutralizing antibodies, nonneutralizing antibodies, secretory antibodies, and cellular immune responses have all been proposed as correlates, and indeed, it may be that all of these play a role, depending on the situation. Differences between data obtained in mice, pigs, or humans contribute to the confusion. The subject has been well reviewed by Franco et al. (49). The bases of neutralizing antibodies are epitopes on two surface proteins of the virus, vp4 and vp7. Soon after initial vaccination, these antibodies, of both the IgG and IgA classes, can be detected by neutralization or by ELISA, and titers of 1/200 or 1/800, respectively, offered protection against rotavirus gastroenteritis (120). Moreover, parenteral immunization with rotavirus virus-like particles (67a, 120) and passive administration to monkeys of IgG can protect (67, 194), clearly implicating antibodies produced in the intestine or diffused into the intestine as effectors. Experiments in mice and pigs suggested that intestinal antibody was more important to protection than serum antibody (16, 104, 129, 198, 199). To add to the confusion, an efficacy study of the pentavalent vaccine showed a correlation between neutralizing antibody against serotype G1, but not against other serotypes, and protection, and in that study, fecal and serum IgA did not correlate with efficacy (26).

On the other hand, the vp6 internal protein of rotaviruses induces a CD4+-mediated lymphocyte proliferation response with production of gamma interferon that correlates with long-term protection after vaccination of mice and pigs (15, 72, 105, 200). Thus, the interim conclusions seem to be that neutralizing antibodies diffused into the intestine or locally produced there are responsible for homotypic short-term protection but that broadening of either antibody or cellular responses in the intestine mediates heterotypic and long-term protection. Despite this complex situation, measurement of serum IgA responses after oral vaccination provides a surrogate for stimulation of intestinal immune responses, and thus protection (49).

Vaccines against human papillomaviruses are based on virus-like particles of the L1 protein that stimulate both type-specific antibodies and cellular responses (135). However, in animal models of vaccination, passive antibody is sufficient for protection (18, 50, 171, 177), and the high level of vaccine efficacy against infection in humans suggests that antibody is the effector mechanism (51, 123). Antibody to papillomavirus can be measured by a pseudovirus-based neutralization assay, as well as by ELISA (131). Serum antibody does transudate into the cervix (111), and as papillomaviruses infect through minor abrasions in the skin and mucosa, it is likely that antibody prevents cell entry at those sites. Efficacy has been maintained for 6 to 7 years postvaccination (152), but although the level of antibody needed for protection and the role of B-cell memory if antibody wanes have not been established (70), studies in animals suggest that very low levels are protective (171).


Rabies is a viral agent introduced into subcutaneous tissue that is transported to the brain and spinal cord after attachment to peripheral nerve axons. However, the virus has a phase of slow replication and persistence in subcutaneous tissues during which prevention of attachment to neurons by antibody is possible (35). Both preexposure and postexposure vaccinations seek to elicit the presence of antibody to the rabies glycoprotein, which is responsible for viral attachment to cellular receptors. The correlate of immunity has been redundantly established as 0.5 IU/ml (a neutralization titer of about 1/5) in experimental animals and verified by absence of failures in humans possessing that level (196).


Zoster consists of a reactivation of varicella virus from latency in dorsal route ganglia owing to loss of cellular immunity due to age or immunosuppression. The vaccine, although composed of live varicella virus, acts through restimulation of flagging cellular responses (84, 122). Accordingly, it is not surprising that CD4+ proliferative responses correlate with protection, although no specific protective value has been established (190). Nevertheless, vaccinees have had reduced incidence of zoster over a period of at least 7 years (162), during which, on average, they have maintained their renewed CD4+ responses to varicella antigen.


This review has shown that after the administration of nearly all vaccines, with the exceptions of BCG and zoster, prevention of infection correlates with the induction of specific antibodies. However, the situation is far from simple: antibodies must be present at the site of replication on the mucosae or in specific organs and must have sufficient breadth to affect heterologous serotypes, if they exist. Moreover, CD4+ responses, key to B-cell help and cytokine production, are sometimes better correlates of protection than antibody titers. Although I have sought to identify single correlates, for many of the vaccines considered above, multiple immune responses interact to protect. B-cell memory is crucial to prolonged protection after vaccination and is dependent on the magnitude of the innate immune response that enhances adaptive cellular responses (21). Nevertheless, the generalization holds that antibodies prevent infection whereas cellular responses control infection once replication has been established. It is likely that vaccines of the future, such as those for HIV, will obey the same paradigm (61, 83, 140).


I thank the following colleagues who reviewed parts of this paper: Ann Gershon, Diane Griffin, Scott Halstead, Keith Klugman, Paul Offit, and Mark Slifka.


[down-pointing small open triangle]Published ahead of print on 12 May 2010.


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