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Figure 1

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Representative results from PCR based Hpall restriction enzyme assay. A 282 bp PCR product is seen following incubation with Hpall in a methylated DTC (B: H), but not in an unmethylated DTC (A: H), as the methylated restriction site (CCGG) is resistant to digestion by the enzyme. All 65 NCE were unmethylated in the FHIT promoter region, while 16 of 65 DTC were methylated. MW: molecular weight ladder; W, water; U, undigested; H, incubated with Hpall; M, incubated with Mspl.

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