MCD1 simplex cells are more sensitive than the WT to IR as G2/M but not G1 (stationary) phase cells.
(A) Nocodazole-arrested WT RAD50
simplex cells (G2
/M) or (B) WT and MCD1
cells from 3 day stationary cultures (G1
) were irradiated with the indicated doses. Cells were spread on YPDA (described in Materials and Methods
under “Nocodazole arrest, gamma irradiation, and post irradiation incubation”) or complete synthetic media plates (there was no difference in survival between the two types of media). Survival was determined after 2–3 days. Survival was determined from at least 6 cultures for each genotype; error bars correspond to the standard error (SEM).
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