Search tips
Search criteria 


Logo of lungindiaHomeCurrent issueInstructionsSubmit article
Lung India. 2010 Apr-Jun; 27(2): 63–65.
PMCID: PMC2893427

FEV6 as screening tool in spirometric diagnosis of obstructive airway disease



The use of spirometry is currently limited to the diagnosis of obstructive airway disease for tertiary centers mainly because of the unmet need for technical expertise and funding. Use in primary care asks for a simpler and cost-effective screening tool for obstructive airway disease.


To estimate the efficacy of FEV6 against the current standard of FVC in the spirometric diagnosis of obstructive airway disease.

Setting and Design:

The Pulmonary Function Laboratory of a tertiary care hospital in Coastal South India. It was a descriptive study.

Materials and Methods:

We analyzed 150 serial patients on ATS standardized spirometers. The patients were classified into normal subjects and those with airway obstruction, further categorized as mild, moderate and severe and those with mixed defect. Those with obstruction were also classified as having reversible and irreversible defects.

Statistical Analysis:

Data was analyzed using SPSS Software (v.11.5), statistical test ANOVA and Pearson correlation was done and P less than 0.05 considered statistically significant.


FVC and FEV6 showed a linear correlation in all subjects. The difference in means was statistically significant in all subjects. The sensitivity and specificity of FEV1/FEV6 in comparison to FEV1/FVC were both found to be 100%.


FEV6 is an excellent screening tool in the diagnosis of airway obstruction but, there is a necessity for further research to confirm our findings. There is also a need for reference values in an Indian setting to find out the efficiency of this new parameter. Our sample size is relatively small and comprises of a very high proportion (70%) of subjects with airway obstruction and so our results may not be applicable for use in general population.

Keywords: Forced vital capacity, forced expiratory maneuver to six-second duration, obstructive airway disease, spirometry


The acceptability criteria for forced vital capacity (FVC) maneuver during PFT have been previously described by American Thoracic Society (ATS): Duration of exhalation should be at least six seconds during which a minimum one second plateau could be reached.[1]

The FVC also has the problem of being dependent on expiratory time in individuals with airway obstruction and in older persons.[2] Studies have suggested that reducing the forced expiratory maneuver to six-second duration (FEV6 ) could replace the FVC maneuver in the diagnosis of airway obstruction.[3,5]

Our aim was to assess the correlation of FVC with FEV6 and the sensitivity and specificity of FEV1/FEV6 as compared to that of FEV1/FVC in the evaluation of airway obstruction.


The study, carried out in the Pulmonary Function Laboratory of a tertiary care hospital in south India, began after approval of institutional ethics committee and informed consent from all the subjects. Data from 150 serial patients referred to the lab from the Chest clinic of the same hospital was analyzed. Spirometry was carried out on a Collins Eagle Flow based spirometer. The system was incorporated with Collins Plus-SQL2000 software for spirometry. The software had the provision to calculate FEV6 in addition to FVC in the same manoeuvre. Patients were asked to withhold any rescue medication (short-acting bronchodilators, long-acting bronchodilators or caffeine derivatives) 12 hours prior to the spirometry. Subjects were tested while seated and all the procedures were carried out in accordance with ATS criteria.[6] Height was measured to the nearest centimeter and weight was rounded off to the nearest kilogram.

Patients were categorized as having "airway obstruction" or "no airway obstruction" by using FEV1/FVC as the gold standard for diagnosis. Those with airway obstruction were further categorized as having a purely obstructive defect or mixed defect (obstruction with restriction). Those with spirometry showing pure restriction were excluded from the study as the correlation between FVC and FEV6 in those with restriction is yet to be validated and beyond the scope of our study. The severity of airway obstruction was graded into three categories: Mild (FEV1 80-60% predicted), Moderate (FEV1 60-40% predicted), Severe (FEV1 less than 40% predicted).[7] Patients were also categorized as having reversible or irreversible airway obstruction with reversibility defined as a 12% and second 200 ml improvement after administration of a short acting bronchodilator (Salbutamol 100 microgram via Metered Dose Inhaler).

The data, thus collected, was fed into Microsoft Excel. It was analysed using Statistical Package for Social Sciences 11.5 for Windows. Pearson correlation and ANOVA were used for statistical analysis. Data were reported as means plus/minus standard deviation.


Baseline characteristics of the study population are given in Table 1. The sex distribution of the study subjects were 51.3% males. In all, 74.03% of the males and 67.12% of females presented with abnormal spirograms, either with obstruction or a mixed defect.

Table 1
Baseline characteristics

The difference between the FVC and the FEV6 values was found to be 13.20 plus/minus 19.71 ml with a range of 0-150 ml. Only in a solitary case, the difference was greater than 100 ml. The distribution of all subjects according to FVC-FEV6 is given in Table 2.

Table 2
Distribution of all subjects according to FVC-FEV6(n = 150)

When those with no airway obstruction were considered separately, it was found that the difference between FVC and FEV6 was 7.73 plus/minus 7.43 ml with a range of 0-40 ml. However, when those with airway obstruction were considered, the difference increased to 15.47 plus/minus 22.60 ml with a wider range of 0-150 ml. This difference in means between those with and without airway obstruction was found to be statistically significant. Among those with airway obstruction, the difference was found to increase with increasing severity Table 3. When ANOVA was carried out with FVC and FEV6, the difference in means was found to be significant in all the subjects irrespective of the obstruction.

Table 3
Variation of FVC-FEV6 according to severity

FVC and FEV6 showed a linear correlation in all the subjects including those with reversible and irreversible obstruction and those with a mixed defect [Table 4].

Table 4
Correlation between FVC and FEV6

Upon comparison of the unadjusted values of FEV1/FEV6 to FEV1/FVC for diagnosing airway obstruction, both sensitivity and specificity of FEV1/FEV6 were 100%. The positive predictive value as well as negative predictive value were 100% as well.


Guidelines state that airway obstruction is defined by a low FEV1/FVC. Therefore, a fault in the measurement of either of these values will lead to misclassification. To avoid errors in measurement of the FVC, the 1994 ATS recommendation stated that, to be considered acceptable, each maneuver should last until a plateau is achieved on the volume–time graph.[6] Patients with airway obstruction often fail to meet the end of test (EOT) criterion defined by a less than-20 ml change in the final two seconds of the maneuver.[8]

Failure to attain acceptable EOT plateaus is relatively common in clinical practice. This may be due to a variety of reasons like, lack of proper technical training, poor motivation of subjects to keep blowing, a faulty spirometer, subjects with severe disease etc. These in turn lead to an under-estimation of the FVC value leading to misclassification. The closer the FEV1/FVC value to Lower limit of normal, greater is the likelihood of missing early obstructive disease. We also found that, the difference in FVC and FEV6 increased with increasing severity. This factor could prove to be a drawback when it is attempted to replace FVC with FEV6 as it can lead to further misclassification.

With an accuracy of 100%, FEV1/FEV6 is an excellent alternative to FEV1/FVC in the diagnosis of airway obstruction. Comparing our results with those of Swanney et al. we obtained higher levels of sensitivity and specificity (100% and 100% in ours, vs. 95.0% and 97.4% in theirs).[2] This was also the case when compared to Vandevoorde et al. who obtained a sensitivity of 94% and specificity of 93.1%.[4] This may probably be because we used absolute values of FEV1/FEV6 in the diagnosis of obstruction without replacing them with reference values in the diagnosis of obstruction unlike the aforementioned studies.

The ATS recommends caution in the diagnosis of cases with values closer to the LLN because both results and estimation of thresholds can shift over to the other side very easily.[9] The interpretation of such results should include clinical information also. This difficulty in making the diagnosis was not evident in our study. This was probably due to the small sample size when compared to other studies[4,5,10] and the high prevalence of airway obstruction among our subjects. Further, many of our subjects were already diagnosed of airway obstruction and were on treatment and there were very few naïve patients.

Both FVC and FEV6 showed an excellent correlation further compounding the hypothesis that FEV6 is an excellent substitute in the diagnosis of airway obstruction. The disparities that could have arisen due to results being closer to normal limits can be avoided by the use of appropriate reference values for FEV1/FEV6. These have already been suggested by Hankinson et al.[11] for an American population and by Garcia-Rio F et al. for a European population.[12] However, reference values are currently unavailable for an Indian population and could limit the usage of FEV6 in clinical practice.

FEV1/FEV6 would be a very effective tool in the primary care scenario for screening and early detection of COPD among high risk patients, i.e, smokers over 45 yrs of age. The use of FEV6 instead of FVC has many distinct advantages: (a) it is easier for the technician and the patient, especially older patients with severe obstructive disease;[13] (b) there is a more discreet and precise end- of- test definition;[13] (c) there is some evidence that FEV6 is more reproducible than FVC;[2] (d) shorter maneuvers reduce the risk of syncope;[13] (e) it reduces the overall time taken to perform a spirometry;[13] (f) it reduces the need for spirometers with very accurate flow detection sensors as required at the end of the FVC maneuver.[2,13] All these advantages can make spirometry a relatively cost-effective and both patient/technician-friendly test to perform.

Most of the spirometers in manufacture, until now, are capable of measuring only FVC and not FEV6. This is a major handicap and probably a reason why such studies haven't been carried out in an Indian setting before.

Time was the major constraint we faced in this study as it had to be completed within a stipulated period of two months. This led to a limitation both in the number of subjects and the gamut of cases we might have obtained. We feel there were not enough cases near the LLN to compare sensitivity and specificity of FEV6 with respect to FVC.


Source of Support: Nil

Conflict of Interest: None declared.


1. American Thoracic Society. Standardization of spirometry: 1994 update. Am J Respir Crit Care Med. 1995;152:1107–36. [PubMed]
2. Swanney MP, Jensen RL, Crichton DA, Beckert LE, Cardno LA, Crapo RL. FEV6 is an acceptable surrogate for FVC in the spirometric diagnosis of airway obstruction and restriction. Am J Respir Crit Care Med. 2000;162:917–9. [PubMed]
3. Demir T, Ikitimur HD, Koc N, Yildirim N. The role of FEV6 in detection of airway obstruction. Respir Med. 2005;99:103–6. [PubMed]
4. Vandevoorde J, Verbanck S, Schuermans D, Kartounian J, Vincken W. FEV1/FEV6 and FEV6 as alternative for FEV1/FVC and FVC in the spirometric detection of irway obstruction and restriction. Chest. 2005;127:1560–4. [PubMed]
5. Akpinar-Elci M, Fedan KB, Enright PL. FEV6 as a surrogate for FVC in detecting airways obstruction and restriction in workplace. Eur Respir J. 2006;27:374–7. [PubMed]
6. Aaron SD, Dales RE, Cardinal P. How accurate is spirometry at predicting restrictive pulmonary impairment? Chest. 1999;115:869–73. [PubMed]
7. Williams EM, Madgwick RG, Morris MJ. Tidal expired airflow patterns in adults with airway obstruction. Eur Respir J. 1998;12:1118–23. [PubMed]
8. Stoller JK, Buist AS, Burrows B, Crystal RG, Fallat RJ, McCarthy K, et al. Quality control of spirometry testing in the registry for patients with severe alpha1-antitrypsin deficiency. Chest. 1997;111:899–909. [PubMed]
9. Medical Section of the American Lung Association. Lung function testing: Selection of reference values and interpretative strategies. Am Rev Respir Dis. 1991;144:1202–18. [PubMed]
10. Rosa FW, Perez-Padilla R, Camelier A, Nascimento OA, Menezes AM, Jardim JR. Efficacy of the FEV1/FEV6 ratio compared to the FEV1/FVC ratio for the diagnosis of airway obstruction in subjects aged 40 years or over. Braz J Med Biol Res. 2007;40:1615–21. [PubMed]
11. Hankinson JL, Odencrantz JR, Fedan KB. Spirometric reference values from a sample of the U.S. general population. Am J Respir Crit Care Med. 1999;159:179–87. [PubMed]
12. Garcia-Rio F, Pino JM, Dorgham A, Alonso A, Villamor J. Spirometric reference equations for European females and males aged 65-85 yrs. Eur Respir J. 2004;24:397–405. [PubMed]
13. Ferguson GT, Enright PL, Buist S, Higgins MW. Office spirometry for lung health in adults. A consensus statement from the National Lung Health Education Program. Chest. 2000;117:1146–61. [PubMed]

Articles from Lung India : Official Organ of Indian Chest Society are provided here courtesy of Medknow Publications