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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Angew Chem Int Ed Engl. Author manuscript; available in PMC 2010 June 25.
Published in final edited form as:
PMCID: PMC2892041
NIHMSID: NIHMS203216

Photothermal Effects of Supramolecularly Assembled Gold Nanoparticles for the Targeted Treatment of Cancer Cells**

Noble-metal nanostructures with unique photophysical properties have been considered as prime candidate agents for the photothermal treatment of cancer.[14] Typically, the photothermal properties of these nanostructures can be controlled by manipulating their sizes and shapes.[4,5] Over the past decade, significant endeavors have been devoted to the production of a variety of gold nanostructures, such as nanoparticles,[6,7] nanoshells,[810] nanorods,[11,12] and nanocages,[5,13,14] which are able to overcome limitations of organic-dye-based photothermal agents,[7] such as low light absorption and undesired photobleaching. For sufficient energy to be harvested/generated to damage tumor cells, the size of these nanostructure-based agents are required in the range of tens to hundreds nm.[15] However, the relatively “large” size of the agents often leads to poor bioclearance (i.e., accumulation in the liver, spleen, and kidneys), which is a major obstacle to their in vivo application.[1618] Alternatively, the photophysical properties of noble-metal nanostructures can be altered systematically by the formation of aggregates through self-assembly.[1930] The antibody-assisted aggregation of Au nanoparticles on cell membranes or in intracellular environments led to the enhancement of photothermal performance[31] as a result of the collective effects[32,33] associated with the assembled structures. Therefore, the self-assembly of small noble-metal building blocks, that is, noble-metal colloids with diameters of less than 8 nm[1618] (compatible with renal clearance) would be a promising approach toward a new class of noble-metal photothermal agents.

Recently, we demonstrated a convenient, flexible, and modular self-assembly approach for the preparation of supramolecular nanoparticles (SNPs) of controlled size through multivalent molecular recognition based on β-cyclodextrin (CD) and adamantane (Ad) motifs.[34] Size-controlled SNPs were prepared by mixing three molecular building blocks: 1) an Ad-grafted polyamidoamine dendrimer with a diameter of approximately 1.9 nm, 2) CD-grafted branched polyethylenimine (CD-PEI), and 3) Ad-grafted polyethylene glycol (Ad-PEG). We hypothesized that such a supramolecular synthetic approach could be further explored to assemble inorganic building blocks (i.e., 2 nm Au colloids) into a collection of Au supramolecular nanoparticles (Au-SNPs) with defined sizes. We anticipated that the resulting Au-SNPs might exhibit enhanced photothermal effects[19] and could thus be promising candidate agents for photothermal cancer treatment. The use of a supramolecular approach enables[34,35] the convenient incorporation of targeting ligands to provide target-specific Au-SNPs.

In this study, we adopted a supramolecular approach to prepare size-controlled Au-SNPs for use as a new type of photothermal agent from three building blocks: Ad-grafted 2 nm Au colloids, CD-PEI, and Ad-PEG (Figure 1). We used transmission electron microscopy (TEM) and zeta-potential measurements to characterize the size/morphology and surface charge densities, respectively, of the resulting Au-SNPs. We carried out further studies to unveil the unique physical properties of the Au-SNPs, including 1) their stability at different temperatures and pH values, 2) their size-dependent photophysical properties, and 3) their thermally induced disassembly. Moreover, laser-induced microbubble-generation experiments were performed with the 118 nm Au-SNPs to demonstrate their significantly enhanced photothermal effects relative to those of the 2 nm Au colloids. We used arginine–glycine–aspartic acid (RGD) peptide as a targeting ligand and αvβ3-positive/negative cells as the corresponding biological system to test the specificity and selectivity of RGD-Au-SNPs, which were generated from Au-SNPs by dynamic in situ ligand exchange,[35] and observed selective damage of the αvβ3-positive cells (no damage of neighboring αvβ3-negative cells).

Figure 1
Supramolecular synthetic approach for the preparation of size-controlled gold supramolecular nanoparticles (Au-SNPs). A molecular-recognition system based on adamantane (Ad) and β-cyclodextrin (CD) was employed to assemble three building blocks: ...

The three molecular building blocks, Ad-grafted 2 nm Au colloids (Figure 2a), CD-PEI, and Ad-PEG, were prepared and characterized as shown in the Supporting Information. We then prepared size-controlled Au-SNPs (Figure 2b) by slowly adding phosphate-buffered saline (PBS, pH 7.2) with Ad-grafted 2 nm Au colloids (0.0213 mgmL−1) to PBS solutions with variable amounts of CD-PEI (0.0725–5.73 mgmL−1) and Ad-PEG (4.2 mgmL−1), followed by incubation at room temperature overnight. By simply tuning the ratio between the Ad-grafted 2 nm Au colloids and CD-PEI, we were able to obtain a collection of Au-SNPs with variable sizes ranging between 40 and 118 nm (Figure 2c). The formation mechanism originally proposed for SNPs[34] can be used to explain the size controllability observed for Au-SNPs. In short, the mixing ratios of the three building blocks altered the equilibrium between the propagation/aggregation of the Au colloid/CD-PEI hydrogel network and the Ad-PEG-induced capping/solvation of the hydrogel network, and changes in this equilibrium led to changes in the size of the Au-SNPs.

Figure 2
Analysis by transmission electron microscopy (TEM). a) Ad-grafted 2 nm Au colloids: the inorganic building blocks of Au-SNPs. b) A single 118 nm Au-SNP obtained by the supramolecular synthetic approach. c) Titration plot showing the relationship between ...

TEM images indicated that the Au-SNPs had spherical shapes with a narrow size distribution (Figure 2b; see also the Supporting Information). Figure 2b shows a highly magnified typical TEM image of a single Au-SNP; individual 2 nm Au colloids are clearly visible. Zeta-potential measurements (Zetasizer Nano, Malvern Instruments) revealed that the Au-SNPs carried surface charge densities covering the range of 11–20 mV (see Figure S1 in the Supporting Information) as a result of the incorporation of positively charged CD-PEI. Since the Au-SNPs were prepared by supramolecular assembly, we characterized their dynamic stability under different environmental conditions. We monitored the size variation of 118 nm Au-SNPs in PBS by TEM[36] at temperatures ranging from 7 to 100°C (Figure 2d) and at pH values from 3 to 10 (Figure 2e). The results indicated that 118 nm Au-SNPs are stable in PBS at 7–40°C and pH 5–10. Also, Au-SNPs can maintain their size and morphology in PBS either with or without 10% serum, most likely because the “stealth” effect imparted by PEG grafted on the outside of the Au-SNPs could lower the further agglomeration of particles.[37] These results suggest that Au-SNPs can be used under physiological conditions. At higher temperatures (> 50°C, Figure 2d), they dissociated into small fragments, and a broader size distribution of the Au-SNPs was observed by TEM. Complete disassembly of the 118 nm Au-SNPs into 2 nm Au colloids was observed when the temperature was increased to 100°C. The thermal disassembly of 118 nm Au-SNPs can be attributed to the weakened Ad/CD supramolecular interactions at elevated temperatures.[38]

To test the feasibility of the application of Au-SNPs as photothermal agents, we chose 118 nm Au-SNPs as the model system.[39] For comparison, 2 nm Au colloids were employed as a control. First, we investigated the photophysical properties of 118 nm Au-SNPs and 2 nm Au colloids by UV/Vis spectroscopy (Figure 3a). Given the characteristic surface-plasmon-resonance absorption of Au-SNPs and Au colloids (between 500 and 530 nm),[19] we chose a 532 nm green pulsed laser to test their photothermal effects and performed laser-induced microbubble-generation studies to monitor the locally accumulated heat of individual Au-SNPs. We tested a broad range of energy densities (3–265 mJcm−2) of a 532 nm pulsed laser with a 6 ns pulse duration. Au-SNPs and Au-colloid suspensions in PBS (with a normalized Au concentration of 4.67 mgmL−1) were irradiated with the laser at different energies. For the 118 nm Au-SNPs, a laser threshold of 32 mJcm−2 was sufficient for the generation of microbubbles upon laser irradiation (Figure 3b). In contrast, no microbubbles were observed for 2 nm Au colloids even at the maximum laser energy tested (265 mJcm−2; Figure 3c). The significant enhancement of the photothermal effects in 118 nm Au-SNPs can be attributed to the collective heating effect[33] in Au-SNPs. Interestingly, we also found that the efficiency of the collective heating effect was size-dependent: 40 nm Au-SNPs exhibited a threshold of 61 mJcm−2.

Figure 3
a) UV/Vis absorption spectra of 2 nm Au colloids and 118 nm Au-SNPs. b,c) Time-resolved bright-field micrographs of suspensions of 118 nm Au-SNPs (b) and Ad-grafted 2 nm Au colloids (c) during the scanning of a pulsed laser (6 ns, 532 nm; 32 mJcm−2 ...

The formation of explosive vapor bubbles on individual Au-SNPs requires an elevated local temperature higher than the critical temperature of the liquid medium (374°C for water).[40] We hypothesize that upon the formation of microbubbles, the localized accumulated heat could facilitate the thermal disassembly of Au-SNPs into smaller fragments, in a process similar to that observed for 118 nm Au-SNPs when the temperature was above 100°C (Figure 2d). To monitor laser-induced Au-SNP disassembly, we used a pulsed laser (with a 6 ns pulse duration) to irradiate Au-SNPs at a repetition rate of 1 Hz. Microbubble formation was captured by the time-resolved imaging setup 70 ns after the arrival of the laser pulse. We observed a dramatic decrease in the number of laser-induced microbubbles after irradiation with several laser pulses. Thus, most of the Au-SNPs in the solution appeared to have thermally disassembled into smaller fragments, which attenuates their photothermal characteristics.[7,19] In the following studies on photothermal treatment, a fixed laser power of 120 mJcm−2 was used to ensure microbubble formation on the 118 nm Au-SNPs.

Through the incorporation of targeting ligands, photothermal agents based on Au nanostructures can be utilized for the targeted photothermal treatment of certain types of cancer cells.[6,12,41,42] In our study, RGD-Au-SNPs that could recognize tumor cells with membrane αvβ3 integrin receptors were produced by dynamic ligand exchange (Ad-PEG-RGD (0.21 mg) was added to a solution (1.0 mL) of 118 nm Au-SNPs (4.67 mgmL−1); see the Supporting Information).[43] The 118 nm RGD-Au-SNPs were used along with the controls (RGD-grafted 2 nm Au colloids and nontargeting 118 nm Au-SNPs) for targeted photothermal treatment in four-well chamber slides containing both αvβ3-positive U87 glioblastoma cells and αvβ3-negative MCF7 breast cancer cells. To make it easier to visually distinguish the two different types of cells, we labeled the U87 and MCF7 cells with green and red fluorescent dyes (DiO and DiD cell-labeling solution, Invitrogen), respectively. After incubation for 20 min with the three agents and subsequent replacement of the culture media (to remove the free agents), the cells in the culture chambers were exposed to pulsed laser irradiation (6 ns, 120 mJcm−2) with a beam diameter of 1 mm (a photomask was used; see the Supporting Information). The irradiated cells were kept in an incubator (5% CO2, 37°C) for 2 h, during which time the cells damaged by microbubble formation could detach from the substrates. An inverted fluorescence microscope (Nikon TE2000) was employed to examine the cells within the irradiated regions. Cell detachment was observed for the U87 cells treated with RGD-Au-SNPs (Figure 4a). In contrast, negligible cell detachment was observed for MCF7 cells treated with RGD-Au-SNPs (Figure 4b) as well as for both types of cells treated with non-targeted Au-SNPs. These results suggest that 1) RGD peptide confers target specificity to the Au-SNPs to enable the photothermal treatment of ανβ3-positive U87 cells, and 2) nontargeting Au-SNPs have no significant effect on cancer cells, as their surface-grafted PEG chains[44] are capable of reducing nonspecific binding to cells. Furthermore, no cell detachment was detected for the U87 cells treated with Au colloids (Figure 4c); this result validated our previous observation that 2 nm Au colloids exhibit minute photothermal effects at the given pulsed laser irradiation.

Figure 4
a–c) Fluorescence micrographs of U87 cells (αvβ3 +, labeled green) treated with 118 nm RGD-Au-SNPs (a), MCF7 cells (αvβ3-, labeled red) treated with 118 nm RGD-Au-SNPs (b), and U87 cells treated with RGD-grafted ...

To demonstrate the selectivity of RGD-Au-SNPs for target-specific photothermal treatment, we investigated the targeted depletion of αvβ3-positive cells in a cell mixture containing both αvβ3-positive U87 and αvβ3-negative MCF7 cells. We treated a 1:1 cell mixture (Figure 4d, middle) containing U87 cells (green) and MCF7 cells (red) with RGD-Au-SNPs (4.67 mgmL−1). After the removal of free RGD-Au-SNPs, the cell mixture was irradiated with a pulsed laser. In the irradiated region, U87 cells (green) were depleted, and the remaining MCF7 cells (red) were able to be continuously cultured on the substrates (Figure 4d, right). In the region outside the laser footprint, both the positive and the negative cells remained. These results suggest that the photothermal treatment of RGD-Au-SNPs is highly selective for targeted cells. Besides the specific targeting effects of RGD-Au-SNPs, the use of a pulsed laser led to effective photothermal effects in a defined location within a nanosecond time frame and thus enabled localized cell damage in a spatially confined fashion. In contrast, the previous use of continuous-wave radiation often led to a large area of cell damage as a result of heat diffusion from the targeted cells to the surrounding medium over the relatively long period of light irradiation.[45]

The cell-damage mechanism is based on the mechanical destruction associated with the formation of explosive microbubbles. It is similar to those proposed for photothermal agents based on Au nanoparticles[6,46,47] or carbon nanotubes,[48] but is dramatically different from the heating-damage mechanism observed for other photothermal agents.[7,8,41,49] To visualize the microbubble-induced mechanical destruction[46] of the targeted cell, we used time-resolved imaging to monitor how an RGD-Au-SNP-grafted cell responded to microbubble formation right after laser irradiation. To ensure that a small number of RGD-Au-SNPs were grafted on the cells, we treated the αvβ3-positive U87 cells with 118 nm RGD-Au-SNPs at low concentration (0.93 mgmL−1). Figure 4e shows the time-dependant response of a U87 cell treated with RGD-Au-SNPs to irradiation with the 6 ns pulsed laser (120 mJcm−2). After irradiation, a fast contraction of the cellular protrusion was observed as a result of the localized mechanical destruction caused by a microbubble.

In conclusion, we have successfully demonstrated the synthesis of size-controlled Au-SNPs from 2 nm Au colloids by a supramolecular self-assembly approach. The resulting Au-SNPs exhibited significantly enhanced photothermal effects and were used to demonstrate the targeted photothermal treatment of a subpopulation of cancer cells after the incorporation of target-specific ligands. We envision that 1) such a supramolecular assembly approach could be used to assemble other “small” inorganic nanoparticles (e.g., superparamagnetic oxide nanoparticles[50]) for broader application in materials science and biomedicine; 2) diverse functional building blocks and therapeutic loads (e.g., DNA, proteins, drugs) could be packaged into Au-SNPs, the laser-induced disassembly of which could be used as a controlled release mechanism; and 3) a two-photon laser[51] could be employed to overcome the tissue-penetration limitation.

Supplementary Material

Experimental

Footnotes

**This research was supported by NIH-NCI NanoSystems Biology Cancer Center (U54CA119347).

Supporting information for this article is available on the WWW under http://dx.doi.org/10.1002/anie.201000062.

Contributor Information

Dr. Shutao Wang, Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California, Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770, (USA), Fax: (+1)310-206-8975.

Kuan-Ju Chen, Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California, Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1)310-206-8975.

Ting-Hsiang Wu, Department of Electrical Engineering, University of California, Los Angeles, 420 Westwood Plaza, EIV 37-138, Los Angeles, CA 90095 (USA)

Dr. Hao Wang, Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California, Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1)310-206-8975.

Dr. Wei-Yu Lin, Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California, Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1)310-206-8975.

Minori Ohash, Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California, Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1)310-206-8975.

Pei-Yu Chiou, Department of Mechanical and Aerospace Engineering, University of California, Los Angeles, 420 Westwood Plaza, EIV 37-138, Los Angeles, CA 90095 (USA)

Hsian-Rong Tseng, Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), Institute for Molecular Medicine (IMED), University of California, Los Angeles, 570 Westwood Plaza, Building 114, Los Angeles, CA 90095-1770 (USA), Fax: (+1)310-206-8975, Homepage: http://labs.pharmacology.ucla.edu/tsenglab/

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