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BMC Cancer. 2010; 10: 265.
Published online 2010 June 8. doi:  10.1186/1471-2407-10-265
PMCID: PMC2891639

Methylthioadenosine (MTA) inhibits melanoma cell proliferation and in vivo tumor growth

Abstract

Background

Melanoma is the most deadly form of skin cancer without effective treatment. Methylthioadenosine (MTA) is a naturally occurring nucleoside with differential effects on normal and transformed cells. MTA has been widely demonstrated to promote anti-proliferative and pro-apoptotic responses in different cell types. In this study we have assessed the therapeutic potential of MTA in melanoma treatment.

Methods

To investigate the therapeutic potential of MTA we performed in vitro proliferation and viability assays using six different mouse and human melanoma cell lines wild type for RAS and BRAF or harboring different mutations in RAS pathway. We also have tested its therapeutic capabilities in vivo in a xenograft mouse melanoma model and using variety of molecular techniques and tissue culture we investigated its anti-proliferative and pro-apoptotic properties.

Results

In vitro experiments showed that MTA treatment inhibited melanoma cell proliferation and viability in a dose dependent manner, where BRAF mutant melanoma cell lines appear to be more sensitive. Importantly, MTA was effective inhibiting in vivo tumor growth. The molecular analysis of tumor samples and in vitro experiments indicated that MTA induces cytostatic rather than pro-apoptotic effects inhibiting the phosphorylation of Akt and S6 ribosomal protein and inducing the down-regulation of cyclin D1.

Conclusions

MTA inhibits melanoma cell proliferation and in vivo tumor growth particularly in BRAF mutant melanoma cells. These data reveal a naturally occurring drug potentially useful for melanoma treatment.

Background

Melanoma is a common skin cancer resulting in high morbidity and mortality. The development of effective therapeutics designed to target melanoma has become the recent focus of research to improve the melanoma patient's prognosis.

In mammalian cells, 5'-Methylthio-5'deoxyadenosine (MTA) is formed from decarboxylated S-adenosylmethionine in the biosynthesis of spermidine and spermine, and is cleaved by MTA phosphorylase (MTAP) into adenine and 5'-methylthio-5'deoxyribose-1-phospate, which are used for the salvage of ATP and methionine respectively [1].

The MTAP gene lies on 9p21, close to the gene CDKN2A that encodes the tumor suppressor proteins p16INK4A and p14ARF being widely expressed in normal cells and tissues [2]. The INK4A-ARF locus on chromosome 9p21, (encoding p16INK4a and p14ARF), is often deleted in human melanoma [3]. Interestingly, MTAP and CDKN2A are frequently homozygously co-deleted otherwise, inactivated in tumor cells including melanoma [4], resulting in higher intra and extracellular MTA levels [5]. A wide variety of biological responses to MTA have been reported both in vivo and in cell culture. While physiological intracellular concentrations of MTA in the nM range might have a tumor-supporting role in MTAP deficient-melanoma cells [5], the administration of higher concentrations of MTA (μM) interfere with cell proliferation, lymphocyte activation, tumor development, invasiveness and the regulation of apoptosis [6-10]. Moreover, it has been shown that MTA has a differential effect in normal and transformed cells. While hepatocarcinoma cells undergo apoptosis when treated with MTA, normal hepatocytes and normal human fibroblast remain viable and are protected from okadaic acid-induced programmed cell death [5,10,11]. Importantly, MTA has been tested in mice and rats and found to be non-toxic at high doses even when given over extended periods [12,13].

Although the mechanisms of action of MTA are not fully understood, it has been postulated that the inhibition of polyamine synthesis could be responsible for the cytostatic effects of MTA. Moreover, MTA has been shown to interfere with key cell signaling pathways, being able to inhibit growth-factor induced protein tyrosine phosphorylation and to increase intracellular cAMP levels through the inhibition of cAMP phos-phodiesterase [14,15]. Additionally, MTA inhibits protein methylation, modulating cell signaling and protein expression [9,16,17].

A number of studies have demonstrated the effects of MTA in tumoral cell lines. However, in vivo studies have been restricted to gastroenterological tumor models or brain autoimmune disease. Besides the continuous efforts from the scientific community, there are not effective therapeutic approaches for melanoma treatment. In this study we explored the therapeutic properties of MTA in melanoma treatment. We used several human and mouse melanoma cell lines having different mutational status respect RAS, and BRAF proteins, and investigated the inhibition capabilities of MTA in vitro. We also performed in vivo studies using a mouse melanoma xenograft model showing the effectiveness of MTA in melanoma treatment.

Materials and methods

Cell lines

37-31E mouse melanoma cells were described previously [18-20]. UACC903 cells were a gift from J. Trent (P. Pollock, Tgen, Phoenix, AR, USA). SkMel147 and SKMel103 cells were obtained from M. Soengas (CNIO Madrid, Spain) and MeWo and SKMel28 cells were purchased from the ATCC. 37-31E, SkMel147, SKMel103 and MeWo cells were maintained in DMEM with 10% FBS, penicillin/streptomycin. 37-31E cells were supplemented with EGF (50 ng/ml) (Invitrogen, Carlsbad, CA, USA) and Insulin (4 μg/ml) (Invitrogen, Carlsbad, CA, USA) and grown at 37°C and 5% CO2 conditions. UACC903 were maintained in RPMI medium with 10% FBS, penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA).

Antibodies and Western Blot analysis

Cells were lysed in RIPA buffer containing phosphatase and protease inhibitors (Sigma-Aldrich, Saint Louis, MO, USA). Liquid nitrogen frozen tumor samples were homogenized in RIPA buffer. 50 μg of total protein lysates were separated by SDS-PAGE and transferred to a membrane. After blocking, membranes were blotted against different primary antibodies and developed using horseradish peroxidase linked secondary antibodies and ECL (GE Healthcare, Barcelona, Spain). Cyclin D1 antibody was from Santa Cruz; phospho-Erk1/2 (Thr202/Tyr204), cleaved-caspase-3, p-S6 (Ser235/236), and phospho-Akt (Thr308) antibodies were from Cell Signaling (Danvers, MA USA); p-Bad was from Genscript, Piscataway, NJ, USA, Ki67 was from Master diagnostica, (Granada, Spain) and GAPDH was from Trevigen (Gaitherburg, MD, USA). CD31 antibody was from DAKO (Spremberg, Denmark). The anti-MTAP antibody was the generous gift of Dr. D.A. Carson (University of California San Diego, CA, USA), and was used at a 1:1.000 dilution.

Proliferation assays

Cells were seeded one day before treatment (50,000 cells per well (37-31E, MeWo, SkMel103, SkMel147, UACC903,) or 75,000 cells per well (Colo829)). Time point treatments were done in triplicates. Number of viable cells at different time points was analyzed by using Guava-Viacount reagent (Guava Technologies, Hayward, CA, USA), in a cell counter (Viacount; Guava Technologies, Hayward, CA, USA).

Cell cycle analysis

Cells were grown in complete media and treated for 48 h with 10 μM of MTA. Time point treatments were done in triplicates. Then, medium and cells were recollected and after centrifugation, cells were fixed and stained with the Cell Cycle Analysis Guava-Viacount reagent (Guava Technologies, Hayward, CA, USA). Samples were analyzed with the Guava cytometer PCA (Guava Technologies Hayward, CA, USA).

In vivo studies

Five to six month old male FVB/N mice were injected subcutaneously with one million cells in PBS. When tumors reached between 50-100 mm3, mice were treated with either DMSO or MTA (96 μmol/kg body weight), a dose selected for its previously shown efficacy and lack of toxicity in other in vivo models [13,21]. MTA was prepared from S-adenosylmethionine (Europharma, Madrid, Spain) as described elsewhere [22]. Treatments were done by IP injection daily. Control mice were treated with the same volume of DMSO (100 μl). Tumor size and mice weight was monitored every two days. Tumor volume was calculated with the equation (d2*D)*(π/6) (d = small-diameter; D = big-diameter). When mice were sacrificed, tumors were dissected and processed. All the animal procedures have been approved and supervised by the animal care and ethical committee of the Vall d'Hebron Research Institute.

Immunohistochemistry, Immunofluorescence, TUNEL assay and microvessel density quantification

Paraffin-embedded tumor samples were subjected to immunocytochemistry according to the manufacturer's antibody protocol. Samples were developed either by using secondary antibodies linked to horseradish peroxidase (HRP) and diaminobenzidine (DAB) as a substrate or by immunofluorescence. Tumor samples were used to perform a TUNEL assay as described previously [23]. Apoptosis and proliferating cells were quantified by calculating the average of positive cells in ten fields (10×). For microvessel quantification, tumor samples were stained for CD31. Then, the integrated density of CD31 fluorescence per field (20× magnification = 587590 μm2) was measured using ImageJ software (NIH). Ten fields per sample were quantified for a total of 5 DMSO treated tumor samples and 5 MTA treated tumor samples.

Colony formation assays

Three hundred cells were seeded. Treatments were added next day. Plates were incubated at 37°C and 5% CO2 until differences between the treatment conditions were noticeable. Media was changed every 2 days. Plates were washed with PBS, fixed with 4% formaldehyde (Sigma-Aldrich, Saint Louis, MO, USA) in PBS for 10 minutes, and stained with crystal violet. Finally, representative pictures were taken and the number of clones was quantified. At least two biological replicates with three technical replicates each were performed for every cell line.

Motility assays

For motility assays 5 × 104 cells were seeded in a 24 multi-well plate trans-wells (Corning). Following treatment cells were washed with 1× PBS and fixed with 1% glutaraldehyde in PBS. Cells were then stained with an aqueous solution of 0.1% crystal violet. After destaining in water, non-migrating cells in the top of the trans-well were removed, and stained migrating cells in the bottom of the trans-well were destaining with PBS containing 0.2% Triton X-100. The O.D. was then measured at 590 nm[24].

mRNA samples and qRT-PCR

Fresh tumor tissues were disrupted using a rotor-stator homogenizer. mRNAs from tumors and cell lines were purified using RNeasy Kit (Quiagen). Amount and quality of RNA was assessed by spectrometrical measurements. Two hundred ng of RNA per sample were used to obtain cDNA using SuperScript™ III First-Strand Synthesis System for RT-PCR following the manufacturer's recommendations (Invitrogen, Carlsbad, CA USA). qRT-PCR was performed using validated Taqman Probes (VEGF: Mm 01281447_m1, and 18 S RNA: Hs 03003631_g1); (Applied Biosystems, Foster City, CA USA). qRT-PCR was performed according to manufacturers recommendations in a SDS 7900HT System. 18 S RNA was used as an internal control. Results were calculated using ΔΔCt method.

Statistics

Comparisons protein expression and tumor size among cell lines or treatment groups were done by two-sided t test (Microsoft Excel, Microsoft, Redmond, WA, USA). Clonogenic assays were analyzed using Wilcoxon Signed-Rank Test [25](Vassar Stats, Poughkeepsie, NY USA).

Results

Methylthioadenosine (MTA) inhibits melanoma cell proliferation

To investigate the inhibition capabilities of methylthioadenosine (MTA) on melanoma cells, we performed cell proliferation assays. Melanoma cell lines harboring wild type NRAS and BRAF (37-31E and MeWo), NRASQ61L mutation (SKMel 103 and SKMel 147) or BRAFV600E mutation (UACC903, Colo 829) were grown in complete medium and in the presence of increasing concentrations of MTA. Wild type NRAS and BRAF melanoma cells (37-31E, MeWo) (Fig (Fig1A),1A), and melanoma cells harboring NRASQ61L (SKMel 147 and SKMel 103) (Fig (Fig1B)1B) showed a 50% inhibition of cell proliferation at 9.8 ± 0.4 μM, 18.9 ± 1.2 μM, 10.01 ± 0.2 μM and 21.2 ± 0.2 μM respectively, while the GI50 for BRAFV600E mutant cell lines was 6.1 ± 0.4 for UACC903 cells and 8.2 ± 0.3 for Colo 829 cells (Fig (Fig1C).1C). BRAFV600E mutant cells showed a significant lower proliferation rate (p < 0.05) at 96 h using 10 μM MTA compared with wild type or NRASQ61L mutant cells (Fig (Fig1D).1D). All melanoma cell lines were significantly (p < 0.01) more sensitive than normal MEFs (GI50>600 μM) (additional file 1, panel A), reaching the maximum inhibition of proliferation between 10 μM and 600 μM concentration of MTA depending on the cell line (Fig (Fig1).1). Moreover, cells harboring deletions in p16INK4a showed lower levels of MTAP, and 3 out of 4 of these cell lines appeared to be more sensitive to MTA treatment (Fig (Fig1E1E).

Figure 1
Methylthioadenosine inhibits melanoma cell growth. Proliferation assays of (A) 37-31E and MeWo (wild type RAS BRAF), (B) SKMel147 and SKMel103 (NRASQ61L mutated) and (C) UACC903 and Colo829 (BRAFV600E mutated) melanoma cells in complete medium with increasing ...

These results indicate that MTA is effective inhibiting in vitro melanoma cells proliferation. Interestingly, the results also suggest that BRAF mutant cells and probably cells showing low levels of MTAP would be more sensitive to the MTA treatment.

MTA reduces melanoma cells survival

We next investigated whether MTA interferes with melanoma cells survival capabilities. To that end, we performed clonogenic assays in several melanoma cell lines adding increasing concentrations of MTA (1 μM to 1 mM). Interestingly, 1 μM concentration of MTA promoted an increase in the number of colonies in 37-31E cells, with no detectable effects in the other of cell lines tested except for the BRAFV600E mutant cell line Colo829 where 1 μM of MTA totally suppressed the colony formation. At 10 μM of MTA BRAFV600E mutant cell lines were significantly more sensitive (p < 0.02) than RASQ61L mutant cell lines. The addition of 100 μM of MTA, or higher concentrations, was very effective inhibiting melanoma cell viability, independently of the RAS pathway mutational status (Fig (Fig2).2). Altogether, the results showed that at 100 μM or higher concentrations of MTA there was not any difference in viability in response to MTA treatment among cell types harboring wild type or mutant RAS pathway. However, at lower concentrations (10 μM) BRAFV600E mutant cells were slightly more sensitive than wild type or RASQ61L mutant cell lines.

Figure 2
Methylthioadenosine (MTA) decreases melanoma cell viability. Clonogenic assays of 37-31E, MeWo, SKMel147, SKMel103, UACC903 and Colo 829 melanoma cells in complete medium with increasing concentrations of Methylthioadenosine (MTA). Three hundred cells ...

MTA treatment inhibits melanoma in vivo tumor growth

In view of to the inhibitory capabilities of MTA in vitro, we tested whether or not MTA was effective inhibiting in vivo tumor growth. To answer this question we performed an in vivo experiment in a melanoma xenograft mouse model. The FVB/N syngenic 37-31E melanoma cell line was subcutaneously injected into FVB/N mice. Then, mice were divided in two groups and treated with DMSO (control) and MTA respectively. When tumors reached between 50-100 mm3 the drug was administrated daily via intraperitoneal letting the tumors grow for a total of thirty days. Mice treated with MTA showed a significant decrease (47%) in the tumor volume (p < 0.001) compared with the controls (DMSO) (Fig (Fig3A)3A) with no evident toxic effects according to the mice weight (data not shown) and liver function tests (ALT(iu/L): control: 19 ± 2; MTA: 18 ± 3; AST(iu/L): control: 45 ± 4; MTA: 54 ± 3).

Figure 3
Methylthioadenosine (MTA) inhibits in vivo tumor growth and decreases the activation of RAS and PI3K tumor signaling pathways. (A) 37-31E melanoma cell line was subcutaneously injected into immunocompetent FVB/N mice. The animal groups were treated with ...

We investigated the status of p-Erk1/2, p-Akt and p-S6 by immunohistochemistry using paraffin-embedded tumor samples. Tumor samples treated with MTA showed a diminished activity of the PI3K and mTOR pathways according to the p-Erk1/2, p-Akt and p-S6 levels (Fig (Fig3B3B).

Altogether, these results indicate that MTA treatment is effective blocking melanoma in vivo tumor growth interfering with the activation of PI3K and mTOR pathways.

MTA promotes cytostatic effects rather than pro-apoptotic responses

Several publications have demonstrated both the cytostatic and pro-apoptotic effects of MTA in mammalian cells [10,26-28]. In our system, the proliferation curves and in vivo results suggested that MTA might be inhibiting cell proliferation and in vivo tumor growth through cytostatic rather than pro-apoptotic mechanisms. To further confirm this hypothesis we analyzed proliferation markers and the apoptosis levels of the tumor samples and performed in vitro experiments to investigate the effects of MTA in promoting apoptosis.

Immunostaining of the tumor tissues showed that samples from MTA treated mice had lower levels of cyclin D1 expression that closely correlated with a lower tumor proliferation index according to the Ki67 immunostaining (Fig (Fig4A).4A). Apoptosis within the tumor was measured by TUNEL assay and quantification of cleaved-caspase-3 positive tumor cells in paraffin sections. Our results showed that MTA-treated samples had slightly higher levels of apoptotic cells compared with control tumors (Fig (Fig4B).4B). In addition to this, it is known that angiogenesis is directly related to tumor growth. Interestingly, the intra-tumoral expression of VEGF, as well as the basal expression of VEGF in 37-31E cells was down regulated by MTA treatment (Fig (Fig4C).4C). Furthermore, these data correlated with a significant lower vessel density of MTA-treated tumor samples according to CD31 staining (Fig (Fig4C4C).

Figure 4
Methylthioadenosine (MTA) promotes cytostatic effects rather than pro-apoptotic effects in melanoma tumors and cells. (A) Tumor sections from mice treated either with DMSO or MTA were stained with cyclin D1 and Ki67 antibodies. Representative pictures ...

We further analyzed the effect of MTA in vitro. 37-31E cells were treated with 10 μM MTA for different periods of time up to 48 h. At the molecular level the results showed that p-Bad and cleaved-caspase-3 levels did not change significantly over time, indicating the low levels of apoptosis (Fig (Fig4D).4D). However, MTA treatment promoted the de-phosphorylation of the mTOR downstream target ribosomal protein S6 and the down-regulation of the cyclin D1 protein levels, supporting the cytostatic effects of MTA (Fig (Fig4D).4D). Additionally, motility of 37-31E cells was also significantly reduced upon MTA treatment (additional file 1, panel B). Importantly, cell cycle analysis of melanoma cells harboring wild type RAS and BRAF (37-31E), NRASQ61L mutation (SKMel147) and BRAFV600E mutation (UACC903) treated with 10 μM MTA for 48 h, showed an increased number of cells in G1 phase compared with untreated cells (6.6% ± 0.8% (p < 0.05), 8.9% ± 0.5% (p < 0.05) and 16.4% ± 0.7% (p < 0.01) respectively) (Fig (Fig4E).4E). Interestingly BRAFV600E mutant cells UACC903 showed the highest proportion of cells in G1 phase, indicating a slowed down cell cycle

Altogether, these results show evidences indicating that cytostatic effects on tumor cells mediate the reduction in melanoma tumor growth upon MTA treatment.

Discussion

Melanoma is the most serious form of skin cancer. If it is not recognized and treated early, the cancer can advance and spread to other parts of the body, where it becomes hard to treat and can be fatal. Recent studies have provided a much-improved understanding of melanoma biology, however, this knowledge has yet to be translated into effective treatment strategies. In this study, we investigated the therapeutic capabilities of MTA in melanoma treatment, a natural occurring nucleoside that has been shown to be effective in other tumor types [6,10,16,29,30]. Our results show that MTA inhibits in vitro cell proliferation, and viability in a dose dependent manner in a variety of human and mouse melanoma cell lines. Importantly, MTA treatment was also effective inhibiting in vivo tumor growth in a mouse melanoma xenograft model. Furthermore, the molecular analysis of the tumor samples and experiments performed with the cell lines indicated that in our model MTA has mostly cytostatic rather that pro-apoptotic effects.

Besides the inhibitory effects on the polyamine biosynthesis, MTA has been shown to exert other potent and specific pharmacological effects on cellular functions such as proliferation, apoptosis and modulation of the immune system [10,31,32]. Our current findings are in agreement with previous publications showing the inhibitory effects of MTA on proliferation and invasion (Fig (Fig11 and additional file 1) of different types of tumor cells lines [33-35]. It is known that genetic mutations within tumor cells condition the drug response and this could be taken as an advantage in the design of more effective therapeutic approaches. Interestingly, BRAF mutant melanoma cell lines (UACC903 and Colo829) showed the highest sensitivity to MTA treatment, where concentrations of 10 μM of MTA reduced proliferation up to 70% (Fig (Fig1D).1D). Why BRAF mutant cells are more sensitive to MTA treatment is unknown and is an area of our current investigation. Nevertheless, the inhibition of cyclin D1 and proliferation by MTA might have a more pronounced effect in cells addicted to oncogenes with potent mitogenic effects. Additionally, the low levels of MTAP protein expressed in both cell lines might be contributing to the observed response.

In agreement with the in vitro results, MTA treatment reduced in vivo tumor growth by 45%. The molecular analysis of the tumors indicated that MTA treated tumors had lower rates of proliferation according to the Ki67 and cyclin D1 levels, that correlated with lower levels in the PI3K and mTOR pathway activation and VEGF expression. While several studies have shown pro-apoptotic effects of MTA in tumor treatment [10,31], we observed a small increase of apoptosis in our tumor samples.

Recently, it has been described that MTA was able to induce the expression of growth factors and matrix metalloproteases in melanoma cells as well as enhance invasion and vasculogenic mimicry [5]. It is widely known that MTA inhibits methyltransferase enzymatic reactions and interferes with cellular pathways modulating cell signaling and protein expression [5,14,16,17]. In the mentioned study, the obtained data was generated at early time points of MTA treatment. Furthermore, the biological outcome in a long-term treatment, such as melanoma cell proliferation and the in vivo melanoma tumor growth, were not assessed. Our data show that the inhibitory effects of MTA on melanoma cell proliferation occur mostly after 48 h treatment. We believe that the biological response to MTA is dose dependent and cell type dependent. Indeed, several publications have shown that fibroblasts and normal hepatocytes have contrary responses to MTA compared to tumor cells. Moreover, in our system low concentrations of MTA (1 μM) promoted a slight increase in proliferation and viability, supporting a possible cell-type specific differential response to low concentrations of MTA. In mice, after intraperitoneal administration at 75 mg/kg, serum levels of MTA rapidly reached a peak of 28 μM rapidly and, at 30 minutes MTA was still at 10 μM [12]. In our hands a preliminary study of the bioavailability of MTA showed that plasma concentrations 20 min after i.p administration of this compound at 96 μmol/kg (equivalent to 30 mg/kg) were in the micromolar range (20-30 μM) (unpublished observations). In view of the efficacy of MTA reducing in vivo tumor growth, we speculate that the concentration reached within the tumor should be higher than 1 μM.

Previous studies have described a cytostatic effect of MTA on Mewo-LC1, Raji and R1.1 H cells [26-28]. According to our current observations in the xenograft model, MTA promoted cytostatic rather than pro-apoptotic effects. This result was confirmed by the in vitro experiments using the same cell line, where we found that MTA treatment induced the dephosphorylation of the downstream mTOR target S6 ribosomal protein, and the decrease of cyclin D1 protein levels. Importantly, we did not observe any molecular indication of apoptosis. Supporting these results, treatment of melanoma cells with MTA induced a cell cycle slow down in G1 phase. Interestingly, in agreement with the proliferation and viability results, UACC903 BRAF mutant melanoma cells showed the higher accumulation of cells in G1 phase.

MTA is a well-tolerated drug, devoid of the unwanted effects of other methyltransferase inhibitors. It has been administered previously in both acute and chronic experimental models of liver injury and systemic inflammation, showing efficacy and a safe profile [32] with an ID50 of 2.9-0.4 gm/kg (intramuscular) in rats [13]. In humans, MTA is also well tolerated [34,35]. Thus, MTA or any of its synthesized analogs would be good candidates for melanoma treatment in patients

Conclusions

Altogether, here we show the therapeutic potential of the naturally occurring nucleoside MTA in melanoma treatment. Importantly, our results demonstrate that MTA inhibits melanoma cell proliferation and in vivo tumor growth, supporting the antitumoral potential of MTA shown by others in other type of tumors. The data also suggest that BRAFV600E mutant cells appeared to be more sensitive to the cytostatic effects mediated by MTA. This study outlines an efficacious and well-tolerated therapeutic candidate for melanoma intervention.

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

PAP did most of the experimental work and data analysis. AP was involved in the animal work. JG She was involved in the experimental work. JHL, RG and TM performed the pathological and immunohistochemical analysis. JC was involved in the analysis and discussion of the data. MAA contributed with some reagents, analyzed and discussed the data. JAR conceived and designed the study, coordinated the work, anlayzed the data and wrote the paper. All the authors approved and read the final manuscript.

Pre-publication history

The pre-publication history for this paper can be accessed here:

http://www.biomedcentral.com/1471-2407/10/265/prepub

Supplementary Material

Additional file 1:

Figure S1. A) MTA does not inhibit proliferation and viability of normal cells (MEFs). Cell proliferation of mouse embryo fibroblasts (MEFs) in response to increasing concentration of MTA. Medium was changed adding fresh MTA every 48 h. Proliferation assays were performed by counting the number of viable cells using Guava-Viacount reagent (Guava Technologies) in a cell counter (Viacount) at the time points indicated. Clonogenic assays using MEFs in complete medium with increasing concentrations of Methylthioadenosine (MTA). Three hundred cells were seeded. Clones were visualized by crystal violet staining and counted. Fresh media was changed every 48 h. Graphs represent the percentage reduction in the number of clones upon different treatments. p-value was calculated performing a Wilcoxon-signed rank test (Vassar Stats). (B) MTA inhibits motility of 37-31E melanoma cells: 37-31 melanoma cells were seeded in a 24 multi-well plate trans-wells (Corning). Following treatment (either DMSO or MTA 100 μM for the times indicated) cells were washed with PBS and fixed with 1% glutaraldehyde in PBS. Cells were then stained with an aqueous solution of 0.1% crystal violet. After destaining in water, non-migrating cells in the top of the trans-well were removed, and stained migrating cells in the bottom of the trans-well were destaining with PBS containing 0.2% Triton X-100. The O.D. was then measured at 590 nm (Gillies et al., 1986). All the experiments were done in triplicates. p-values were calculated performing a t-student test (*) = p < 0.01.

Acknowledgements

We want to thank Stephan Tenbaum for discussion and reading the manuscript. This work was supported by: Instituto Carlos III, Fondo de Investigaciones Sanitarias projects PI-050227, PI080653 and Fundación Mutua Madrileña (FMM) (from JAR). MAA is supported by the agreement between FIMA and the "UTE project CIMA", Red Temática de Investigación Cooperativa en Cáncer RD06 00200061, and grant FIS PI070392 from Ministerio de Sanidad y Consumo. PAP fellowship was sponsored by DURSI.

References

  • Savarese TM, Ghoda LY, Dexter DL, Parks RE Jr. Conversion of 5'-deoxy-5'-methylthioadenosine and 5'-deoxy-5'-methylthioinosine to methionine in cultured human leukemic cells. Cancer Res. 1983;43(10):4699–4702. [PubMed]
  • Olopade OI, Pomykala HM, Hagos F, Sveen LW, Espinosa R, Dreyling MH, Gursky S, Stadler WM, Le Beau MM, Bohlander SK. Construction of a 2.8-megabase yeast artificial chromosome contig and cloning of the human methylthioadenosine phosphorylase gene from the tumor suppressor region on 9p21. Proc Natl Acad Sci USA. 1995;92(14):6489–6493. doi: 10.1073/pnas.92.14.6489. [PubMed] [Cross Ref]
  • Chin L, Garraway LA, Fisher DE. Malignant melanoma: genetics and therapeutics in the genomic era. Genes Dev. 2006;20(16):2149–2182. doi: 10.1101/gad.1437206. [PubMed] [Cross Ref]
  • Bishop DT, Demenais F, Iles MM, Harland M, Taylor JC, Corda E, Randerson-Moor J, Aitken JF, Avril MF, Azizi E. Genome-wide association study identifies three loci associated with melanoma risk. Nat Genet. 2009;41(8):920–925. doi: 10.1038/ng.411. [PMC free article] [PubMed] [Cross Ref]
  • Stevens AP, Spangler B, Wallner S, Kreutz M, Dettmer K, Oefner PJ, Bosserhoff AK. Direct and tumor microenvironment mediated influences of 5'-deoxy-5'-(methylthio)adenosine on tumor progression of malignant melanoma. J Cell Biochem. 2009;106(2):210–219. doi: 10.1002/jcb.21984. [PubMed] [Cross Ref]
  • Chen H, Xia M, Lin M, Yang H, Kuhlenkamp J, Li T, Sodir NM, Chen YH, Josef-Lenz H, Laird PW. Role of methionine adenosyltransferase 2A and S-adenosylmethionine in mitogen-induced growth of human colon cancer cells. Gastroenterology. 2007;133(1):207–218. doi: 10.1053/j.gastro.2007.03.114. [PubMed] [Cross Ref]
  • Pascale RM, Simile MM, De Miglio MR, Feo F. Chemoprevention of hepatocarcinogenesis: S-adenosyl-L-methionine. Alcohol. 2002;27(3):193–198. doi: 10.1016/S0741-8329(02)00227-6. [PubMed] [Cross Ref]
  • Mato JM, Corrales FJ, Lu SC, Avila MA. S-Adenosylmethionine: a control switch that regulates liver function. FASEB J. 2002;16(1):15–26. doi: 10.1096/fj.01-0401rev. [PubMed] [Cross Ref]
  • Law RE, Stimmel JB, Damore MA, Carter C, Clarke S, Wall R. Lipopolysaccharide-induced NF-kappa B activation in mouse 70Z/3 pre-B lymphocytes is inhibited by mevinolin and 5'-methylthioadenosine: roles of protein isoprenylation and carboxyl methylation reactions. Mol Cell Biol. 1992;12(1):103–111. [PMC free article] [PubMed]
  • Ansorena E, Garcia-Trevijano ER, Martinez-Chantar ML, Huang ZZ, Chen L, Mato JM, Iraburu M, Lu SC, Avila MA. S-adenosylmethionine and methylthioadenosine are antiapoptotic in cultured rat hepatocytes but proapoptotic in human hepatoma cells. Hepatology. 2002;35(2):274–280. doi: 10.1053/jhep.2002.30419. [PubMed] [Cross Ref]
  • Lubin M, Lubin A. Selective killing of tumors deficient in methylthioadenosine phosphorylase: a novel strategy. PLoS One. 2009;4(5):e5735. doi: 10.1371/journal.pone.0005735. [PMC free article] [PubMed] [Cross Ref]
  • Wolford RW, Riscoe MK, Johnson L, Ferro AJ, Fitchen JH. Effect of 5'-methylthioadenosine (a naturally occurring nucleoside) on murine hematopoiesis. Exp Hematol. 1984;12(11):867–871. [PubMed]
  • Simile MM, Banni S, Angioni E, Carta G, De Miglio MR, Muroni MR, Calvisi DF, Carru A, Pascale RM, Feo F. 5'-Methylthioadenosine administration prevents lipid peroxidation and fibrogenesis induced in rat liver by carbon-tetrachloride intoxication. J Hepatol. 2001;34(3):386–394. doi: 10.1016/S0168-8278(00)00078-7. [PubMed] [Cross Ref]
  • Maher PA. Inhibition of the tyrosine kinase activity of the fibroblast growth factor receptor by the methyltransferase inhibitor 5'-methylthioadenosine. J Biol Chem. 1993;268(6):4244–4249. [PubMed]
  • Riscoe MK, Ferro AJ. 5-Methylthioribose. Its effects and function in mammalian cells. J Biol Chem. 1984;259(9):5465–5471. [PubMed]
  • Lee SH, Cho YD. Induction of apoptosis in leukemia U937 cells by 5'-deoxy-5'-methylthioadenosine, a potent inhibitor of protein carboxylmethyltransferase. Exp Cell Res. 1998;240(2):282–292. doi: 10.1006/excr.1998.4000. [PubMed] [Cross Ref]
  • Mowen KA, Tang J, Zhu W, Schurter BT, Shuai K, Herschman HR, David M. Arginine methylation of STAT1 modulates IFNalpha/beta-induced transcription. Cell. 2001;104(5):731–741. doi: 10.1016/S0092-8674(01)00269-0. [PubMed] [Cross Ref]
  • Recio JA, Merlino G. Hepatocyte growth factor/scatter factor activates proliferation in melanoma cells through p38 MAPK, ATF-2 and cyclin D1. Oncogene. 2002;21(7):1000–1008. doi: 10.1038/sj.onc.1205150. [PubMed] [Cross Ref]
  • Esteve-Puig R, Canals F, Colome N, Merlino G, Recio JA. Uncoupling of the LKB1-AMPKalpha energy sensor pathway by growth factors and oncogenic BRAF. PLoS One. 2009;4(3):e47711. doi: 10.1371/journal.pone.0004771. [PMC free article] [PubMed] [Cross Ref]
  • Lopez-Fauqued M, Gil R, Grueso J, Hernandez J, Pujol A, Moline T, Recio JA. The dual PI3K/mTOR inhibitor (PI-103) promotes immunosupression, in vivo tumor growth and increases survival of sorafenib treated melanoma cells. Int J Cancer. 2009. [PubMed]
  • Moreno B, Hevia H, Santamaria M, Sepulcre J, Munoz J, Garcia-Trevijano ER, Berasain C, Corrales FJ, Avila MA, Villoslada P. Methylthioadenosine reverses brain autoimmune disease. Ann Neurol. 2006;60(3):323–334. doi: 10.1002/ana.20895. [PubMed] [Cross Ref]
  • Schlenk F, Ehninger DJ. Observations on the Metabolism of 5'-Methylthioadenosine. Arch Biochem Biophys. 1964;106:95–100. doi: 10.1016/0003-9861(64)90161-4. [PubMed] [Cross Ref]
  • Kingsley-Kallesen M, Mukhopadhyay SS, Wyszomierski SL, Schanler S, Schutz G, Rosen JM. The mineralocorticoid receptor may compensate for the loss of the glucocorticoid receptor at specific stages of mammary gland development. Mol Endocrinol. 2002;16(9):2008–2018. doi: 10.1210/me.2002-0103. [PubMed] [Cross Ref]
  • Gillies RJ, Didier N, Denton M. Determination of cell number in monolayer cultures. Anal Biochem. 1986;159(1):109–113. doi: 10.1016/0003-2697(86)90314-3. [PubMed] [Cross Ref]
  • Wilcoxon F. Individual Comparisons by Ranking Methods. Biometrics Bulletin. 1945;1(6):80–83. doi: 10.2307/3001968. [Cross Ref]
  • Yamanaka H, Kubota M, Carson DA. Synergistic inhibition of polyamine synthesis and growth by difluoromethylornithine plus methylthioadenosine in methylthioadenosine phosphorylase-deficient murine lymphoma cells. Cancer Res. 1987;47(7):1771–1774. [PubMed]
  • Nishikawa S, Ueno A, Inoue H, Takeda Y. Effect of 5'-difluoromethylthioadenosine, an inhibitor of methylthioadenosine phosphorylase, on proliferation of cultured cells. J Cell Physiol. 1987;133(2):372–376. doi: 10.1002/jcp.1041330223. [PubMed] [Cross Ref]
  • Liteplo RG, Jurewicz TJ. Serum has a differential effect on DNA replication in a human melanoma cell line cultured in methionine or 5'-deoxy-5'-methylthioadenosine. Biochim Biophys Acta. 1991;1088(3):365–372. [PubMed]
  • Basu I, Cordovano G, Das I, Belbin TJ, Guha C, Schramm VL. A transition state analogue of 5'-methylthioadenosine phosphorylase induces apoptosis in head and neck cancers. J Biol Chem. 2007;282(29):21477–21486. doi: 10.1074/jbc.M702287200. [PubMed] [Cross Ref]
  • Li TW, Zhang Q, Oh P, Xia M, Chen H, Bemanian S, Lastra N, Circ M, Moyer MP, Mato JM. S-Adenosylmethionine and methylthioadenosine inhibit cellular FLICE inhibitory protein expression and induce apoptosis in colon cancer cells. Mol Pharmacol. 2009;76(1):192–200. doi: 10.1124/mol.108.054411. [PubMed] [Cross Ref]
  • Avila MA, Garcia-Trevijano ER, Lu SC, Corrales FJ, Mato JM. Methylthioadenosine. Int J Biochem Cell Biol. 2004;36(11):2125–2130. doi: 10.1016/j.biocel.2003.11.016. [PubMed] [Cross Ref]
  • Hevia H, Varela-Rey M, Corrales FJ, Berasain C, Martinez-Chantar ML, Latasa MU, Lu SC, Mato JM, Garcia-Trevijano ER, Avila MA. 5'-methylthioadenosine modulates the inflammatory response to endotoxin in mice and in rat hepatocytes. Hepatology. 2004;39(4):1088–1098. doi: 10.1002/hep.20154. [PubMed] [Cross Ref]
  • Shafman TD, Sherman ML, Kufe DW. Effect of 5'-methylthioadenosine on induction of murine erythroleukemia cell differentiation. Biochem Biophys Res Commun. 1984;124(1):172–177. doi: 10.1016/0006-291X(84)90932-X. [PubMed] [Cross Ref]
  • de Ferra F, Baglioni C. Correlation between growth inhibition and presence of 5'-methylthioadenosine in cells treated with interferon. Cancer Res. 1984;44(6):2297–2301. [PubMed]
  • Riscoe MK, Schwamborn J, Ferro AJ, Olson KD, Fitchen JH. Inhibition of growth but not differentiation of normal and leukemic myeloid cells by methylthioadenosine. Cancer Res. 1987;47(14):3830–3834. [PubMed]
  • Stramentinoli G, Gennari F. Adenosine derivatives of anti- inflammatory and analgesic activity, and therapeutic composi- tions which contain them as their active principle. Stra- mentinoli patent 4,454,122. Filed: Aug 6, 1982; Issued: Jun 12, 1984.
  • Moratti E. Pharmaceutical compositions containing 5_-deoxy- 5_-methylthioadenosine s-adenosylmethionine and their salts for reducing seborrhea. Moratti patent 5,753,213. Filed Mar 13, 1990; Issued May 19, 1998.

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