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Logo of annbotAboutAuthor GuidelinesEditorial BoardAnnals of Botany
 
Ann Bot. Jun 2010; 105(7): 1119–1128.
Published online Mar 17, 2010. doi:  10.1093/aob/mcq049
PMCID: PMC2887069
Transcriptomic analysis reveals differential gene expression in response to aluminium in common bean (Phaseolus vulgaris) genotypes
Dejene Eticha,1 Marc Zahn,1 Melanie Bremer,1 Zhongbao Yang,1 Andrés F. Rangel,2 Idupulapati M. Rao,3 and Walter J. Horst1*
1Institute of Plant Nutrition, Leibniz University Hannover, Herrenhaeuser Str. 2, 30419 Hannover, Germany
2Yara International ASA, Research Centre Hanninghof, Hanninghof 35, 48249 Dülmen, Germany
3CIAT–International Center for Tropical Agriculture, Apartado Aéreo 6713, Cali, Colombia
*For correspondence. E-mail horst/at/pflern.uni-hannover.de
Received December 22, 2009; Revised January 12, 2010; Accepted January 19, 2010.
Abstract
Background and Aims
Aluminium (Al) resistance in common bean is known to be due to exudation of citrate from the root after a lag phase, indicating the induction of gene transcription and protein synthesis. The aims of this study were to identify Al-induced differentially expressed genes and to analyse the expression of candidate genes conferring Al resistance in bean.
Methods
The suppression subtractive hybridization (SSH) method was used to identify differentially expressed genes in an Al-resistant bean genotype (‘Quimbaya’) during the induction period. Using quantitative real-time PCR the expression patterns of selected genes were compared between an Al-resistant and an Al-sensitive genotype (‘VAX 1’) treated with Al for up to 24 h.
Key Results
Short-term Al treatment resulted in up-regulation of stress-induced genes and down-regulation of genes involved in metabolism. However, the expressions of genes encoding enzymes involved in citrate metabolism were not significantly affected by Al. Al treatment dramatically increased the expression of common bean expressed sequence tags belonging to the citrate transporter gene family MATE (multidrug and toxin extrusion family protein) in both the Al-resistant and -sensitive genotype in close agreement with Al-induced citrate exudation.
Conclusions
The expression of a citrate transporter MATE gene is crucial for citrate exudation in common bean. However, although the expression of the citrate transporter is a prerequisite for citrate exudation, genotypic Al resistance in common bean particularly depends on the capacity to sustain the synthesis of citrate for maintaining the cytosolic citrate pool that enables exudation.
Keywords: Aluminium resistance, aluminum, citrate exudation, common bean, MATE, Phaseolus vulgaris, transcriptomic analysis, differential gene expression
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